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Team:Newcastle/Arginine test
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* Plate containing ''Bacillus subtilis'' 168 colonies. | * Plate containing ''Bacillus subtilis'' 168 colonies. | ||
* Flame (streaking) loop | * Flame (streaking) loop | ||
| - | * LB media consisting of arginine | + | * LB media consisting of arginine |
* Auto pipette | * Auto pipette | ||
* Bursen Burner | * Bursen Burner | ||
* Universal Tube | * Universal Tube | ||
| - | * pH | + | * pH indicator paper |
==Procedures== | ==Procedures== | ||
| Line 22: | Line 22: | ||
#Test (2) - LB media with 10 mM of arginine plus ''B. subtilis'' 168 | #Test (2) - LB media with 10 mM of arginine plus ''B. subtilis'' 168 | ||
* Incubate the culture at 37° C with shaking. | * Incubate the culture at 37° C with shaking. | ||
| - | * Record the pH at every 30 min interval.Use 20 ul of the culture and measure the pH using the pH indicator | + | * Record the pH at every 30 min interval.Use 20 ul of the culture and measure the pH using the pH indicator paper. |
Latest revision as of 20:58, 27 October 2010
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Arginine Test
Materials Required
- Plate containing Bacillus subtilis 168 colonies.
- Flame (streaking) loop
- LB media consisting of arginine
- Auto pipette
- Bursen Burner
- Universal Tube
- pH indicator paper
Procedures
- Perform the experiment using aseptic technique.
- Transfer B. subtilis 168 colonies into universal tubes containing 5 ml of LB media and allowed to grow overnight at 37° C.
- Transfer 1 ml of the overnight culture to another universal tube containing 4 ml of the following media:
- Control (1) - LB media
- Control (2) - LB media with 10 mM of arginine
- Control (3) - LB media plus B. subtilis 168
- Test (1) - LB media with 10 mM of arginine plus B. subtilis 168
- Test (2) - LB media with 10 mM of arginine plus B. subtilis 168
- Incubate the culture at 37° C with shaking.
- Record the pH at every 30 min interval.Use 20 ul of the culture and measure the pH using the pH indicator paper.
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