"
Team:Stanford/NotebookPage/6 July 2010
From 2010.igem.org
(Difference between revisions)
Chrisvanlang (Talk | contribs) |
Karpadillo (Talk | contribs) |
||
| (2 intermediate revisions not shown) | |||
| Line 2: | Line 2: | ||
{{Template: Stanford Notebook Calendar}} | {{Template: Stanford Notebook Calendar}} | ||
<div style="width:720px;text-align:justify"> | <div style="width:720px;text-align:justify"> | ||
| + | |||
| + | [https://docs.google.com/document/edit?id=1xnkLfvrwGJuU1Z_9-y3R0topqahNiJUQpdpNOWlK9Ig&hl=en&authkey=CNnS3IEB Agenda] | ||
Alex's References | Alex's References | ||
| Line 13: | Line 15: | ||
#[http://pubs.acs.org/doi/full/10.1021/bi035150b An FHA Phosphoprotein Recognition Domain Mediates Protein EmbR Phosphorylation by PknH, a Ser/Thr Protein Kinase from Mycobacterium tuberculosis] | #[http://pubs.acs.org/doi/full/10.1021/bi035150b An FHA Phosphoprotein Recognition Domain Mediates Protein EmbR Phosphorylation by PknH, a Ser/Thr Protein Kinase from Mycobacterium tuberculosis] | ||
</div> | </div> | ||
| + | |||
| + | <div style=text-align:left justify"> | ||
=Greg's Lab Notebook= | =Greg's Lab Notebook= | ||
| Line 19: | Line 23: | ||
*Used heat-shock transformation protocol for DH5alpha to transform with the following genetic material, left to culture overnight: | *Used heat-shock transformation protocol for DH5alpha to transform with the following genetic material, left to culture overnight: | ||
| - | {| class="wikitable" style="text-align: center | + | |
| + | {| class="wikitable" style="text-align: center;" | ||
|- | |- | ||
! Function !! Part # !! Distribution Location !! Resistance | ! Function !! Part # !! Distribution Location !! Resistance | ||
Latest revision as of 20:27, 12 July 2010
|
|
|
|
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|
|
|
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Alex's References
- [http://www.nature.com/ja/journal/v59/n2/abs/ja200618a.html Self-activation of Serine/Threonine Kinase AfsK on Autophosphorylation at Threonine-168]
- [http://www.ncbi.nlm.nih.gov/pubmed/11952895 afsS is a target of AfsR, a transcriptional factor with ATPase activity that globally controls secondary metabolism in Streptomyces coelicolor A3(2).]
Chris's references
- [http://www3.interscience.wiley.com/journal/118634717/abstract?CRETRY=1&SRETRY=0 EmbR, a regulatory protein with ATPase activity, is a substrate of multiple serine/threonine kinases and phosphatase in Mycobacterium tuberculosis]
- [http://www.ncbi.nlm.nih.gov/pubmed/18424526 The critical role of embC in Mycobacterium tuberculosis.]
- [http://jb.asm.org/cgi/content/abstract/188/8/2936 Transcriptional Control of the Mycobacterial embCAB Operon by PknH through a Regulatory Protein, EmbR, In Vivo]
- [http://pubs.acs.org/doi/full/10.1021/bi035150b An FHA Phosphoprotein Recognition Domain Mediates Protein EmbR Phosphorylation by PknH, a Ser/Thr Protein Kinase from Mycobacterium tuberculosis]
Greg's Lab Notebook
- During weekly meeting got up to speed with what the team’s been doing while I was away
- Used heat-shock transformation protocol for DH5alpha to transform with the following genetic material, left to culture overnight:
| Function | Part # | Distribution Location | Resistance |
|---|---|---|---|
| ribosome binding site | B0032 | P1 2I | Amp |
| forward double terminator | B0015 | P1 23L | Amp + Kan |
| bidirectional double terminator | B0014 | P2 24C | Amp + Kan |
| medium constitutive promoter | J23107 | P1 20A | Amp |
| strong constitutive promoter | J23100 | P1 18C | Amp |
| plasmid backbone | pSB1K3 | P1 5A | Kan |
