Team:Stanford/NotebookPage/6 July 2010
From 2010.igem.org
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Alex's References
- Self-activation of Serine/Threonine Kinase AfsK on Autophosphorylation at Threonine-168
- afsS is a target of AfsR, a transcriptional factor with ATPase activity that globally controls secondary metabolism in Streptomyces coelicolor A3(2).
Chris's references
- EmbR, a regulatory protein with ATPase activity, is a substrate of multiple serine/threonine kinases and phosphatase in Mycobacterium tuberculosis
- The critical role of embC in Mycobacterium tuberculosis.
- Transcriptional Control of the Mycobacterial embCAB Operon by PknH through a Regulatory Protein, EmbR, In Vivo
- An FHA Phosphoprotein Recognition Domain Mediates Protein EmbR Phosphorylation by PknH, a Ser/Thr Protein Kinase from Mycobacterium tuberculosis
Greg's Lab Notebook
- During weekly meeting got up to speed with what the team’s been doing while I was away
- Used heat-shock transformation protocol for DH5alpha to transform with the following genetic material, left to culture overnight:
Function | Part # | Distribution Location | Resistance |
---|---|---|---|
ribosome binding site | B0032 | P1 2I | Amp |
forward double terminator | B0015 | P1 23L | Amp + Kan |
bidirectional double terminator | B0014 | P2 24C | Amp + Kan |
medium constitutive promoter | J23107 | P1 20A | Amp |
strong constitutive promoter | J23100 | P1 18C | Amp |
plasmid backbone | pSB1K3 | P1 5A | Kan |