Team:Stanford/NotebookPage/6 July 2010

From 2010.igem.org

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Agenda

Alex's References

  1. Self-activation of Serine/Threonine Kinase AfsK on Autophosphorylation at Threonine-168
  2. afsS is a target of AfsR, a transcriptional factor with ATPase activity that globally controls secondary metabolism in Streptomyces coelicolor A3(2).

Chris's references

  1. EmbR, a regulatory protein with ATPase activity, is a substrate of multiple serine/threonine kinases and phosphatase in Mycobacterium tuberculosis
  2. The critical role of embC in Mycobacterium tuberculosis.
  3. Transcriptional Control of the Mycobacterial embCAB Operon by PknH through a Regulatory Protein, EmbR, In Vivo
  4. An FHA Phosphoprotein Recognition Domain Mediates Protein EmbR Phosphorylation by PknH, a Ser/Thr Protein Kinase from Mycobacterium tuberculosis

Greg's Lab Notebook

  • During weekly meeting got up to speed with what the team’s been doing while I was away
  • Used heat-shock transformation protocol for DH5alpha to transform with the following genetic material, left to culture overnight:


Function Part # Distribution Location Resistance
ribosome binding site B0032 P1 2I Amp
forward double terminator B0015 P1 23L Amp + Kan
bidirectional double terminator B0014 P2 24C Amp + Kan
medium constitutive promoter J23107 P1 20A Amp
strong constitutive promoter J23100 P1 18C Amp
plasmid backbone pSB1K3 P1 5A Kan