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Team:Queens-Canada/7 October 2010
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(Difference between revisions)
(New page: <h2>October 7, 2010</h2> {{:Team:Queens-Canada/head}} <h3>Digestion-Ligation</h3> Thai and Hao '''eCFP'''::'''unc-54 3'UTR''', '''pOdr...) |
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<h2>October 7, 2010</h2> | <h2>October 7, 2010</h2> | ||
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Backbone treated with CIAP for 30 mins. | Backbone treated with CIAP for 30 mins. | ||
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| + | <h3>[[Team:Queens-Canada/transformation|Microinjection]]</h3> | ||
| + | Hao | ||
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| + | '''pStr-1'''::'''eCFP'''::'''unc-54 3'UTR'''::1A3, '''pHsp-3'''::'''eCFP'''::'''unc-54 3'UTR'''::1A3, '''pFlp-1'''::'''eCFP'''::'''unc-54 3'UTR'''::1A3 | ||
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| + | Co-injected each construct with pRF4 marker. | ||
{{:Team:Queens-Canada/foot}} | {{:Team:Queens-Canada/foot}} | ||
Latest revision as of 03:56, 28 October 2010
October 7, 2010
Digestion-Ligation
Thai and Hao
eCFP::unc-54 3'UTR, pOdr-10::eCFP::unc-54 3'UTR, pGpd-2::eCFP::unc-54 3'UTR, pSra-10::eCFP::unc-54 3'UTR, pFlp-1::eCFP::unc-54 3'UTR, ChR2::eCFP::unc-54 3'UTR, pHsp-3::eCFP::unc-54 3'UTR, pOsm-10::eCFP::unc-54 3'UTR, eYFP::eCFP::unc-54 3'UTR, mCherry::eCFP::unc-54 3'UTR, pStr-220::eCFP::unc-54 3'UTR, pSip-1::eCFP::unc-54 3'UTR, pOdr-1::eCFP::unc-54 3'UTR, pStr-1::eCFP::unc-54 3'UTR, pSB1C3
Backbone treated with CIAP for 30 mins.
Microinjection
Hao
pStr-1::eCFP::unc-54 3'UTR::1A3, pHsp-3::eCFP::unc-54 3'UTR::1A3, pFlp-1::eCFP::unc-54 3'UTR::1A3
Co-injected each construct with pRF4 marker.
