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Team:Queens-Canada/18 June 2010
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| - | + | {{:Team:Queens-Canada/head}} | |
| + | <h2>June 18, 2010</h2> | ||
| + | <h3>[[Team:Queens-Canada/protocols#PCR|PCR]]</h3> | ||
| + | Hao and Yuli | ||
| + | |||
| + | '''pSip-1''', '''pHsp-3''', '''pUnc-47''', '''pRab-7''', '''pMec-7''' with '''BBa_I714891''' | ||
| + | |||
| + | Program run using HIFI with an extension time of 3 min and annealing temperature of 57 °C without primers for 5 cycles. | ||
| + | Primers were then added and program was run with annealing temperature of 1 min 15 s. | ||
| + | |||
| + | <h3>[[Team:Queens-Canada/protocols#Gel Electrophoresis|Gel Electrophoresis]]</h3> | ||
| + | Hao and Yuli | ||
| + | |||
| + | '''pSip-1'''::'''eGFP''', '''pHsp-3'''::'''eGFP''', '''pMec-7'''::'''eGFP''', '''pUnc-47'''::'''eGFP''', '''pRab-7'''::'''eGFP''' | ||
| + | |||
| + | Used 0.7 g agarose in 100 mL TBE. Gel run for 45 mins at 100 V. | ||
| + | |||
| + | <h3>[[Team:Queens-Canada/transformation|Microinjection]]</h3> | ||
| + | Hao and Yuli | ||
| + | |||
| + | Practiced injections with water | ||
| + | |||
| + | {{:Team:Queens-Canada/foot}} | ||
Latest revision as of 01:06, 28 October 2010
June 18, 2010
PCR
Hao and Yuli
pSip-1, pHsp-3, pUnc-47, pRab-7, pMec-7 with BBa_I714891
Program run using HIFI with an extension time of 3 min and annealing temperature of 57 °C without primers for 5 cycles. Primers were then added and program was run with annealing temperature of 1 min 15 s.
Gel Electrophoresis
Hao and Yuli
pSip-1::eGFP, pHsp-3::eGFP, pMec-7::eGFP, pUnc-47::eGFP, pRab-7::eGFP
Used 0.7 g agarose in 100 mL TBE. Gel run for 45 mins at 100 V.
Microinjection
Hao and Yuli
Practiced injections with water
