Team:Alberta/Kit components

From 2010.igem.org

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! Carotenoid Pathway
! Carotenoid Pathway
! Violacein Pathway
! Violacein Pathway
-
! Repressors
+
! Transcription Factors
 +
! Resistance Marker
|-
|-
-
| row 1, cell 1 hello world
+
| GFP
-
| row 1, cell 2
+
| CrtE
-
| row 1, cell 3
+
| VioA
-
| row 1, cell 4
+
| LacI
 +
| Ampicillin
|-
|-
-
| row 2, cell 1
+
| mRFP
-
| row 2, cell 2
+
| CrtB
-
| row 2, cell 3
+
| VioB
-
| row 2, cell 4
+
| AraC
 +
|Chloramphenicol
 +
|-
 +
| CFP
 +
| CrtI
 +
| VioD
 +
| LambdaCI
 +
| Kanamycin
 +
|-
 +
| YFP
 +
| CrtY
 +
| VioE
 +
| TetR
 +
| Tetramycin
 +
 
|}
|}
 +
[[Image:Alberta box.jpg|right|280px|thumb|GENOMIKON's DNA parts]]
 +
'''Linker Parts'''
 +
*Transcription
 +
*5 Anderson Promoters
 +
*Operon Linker
 +
*Anti-linker as a negative control
 +
*Short null-linker for inversion of ends
 +
*Fusion Protein Link
 +
*Protein Tags
-
'''Linker Parts'''
 
'''Other Parts'''
'''Other Parts'''
 +
*High, medium and low copy number Oris
 +
*Caps
 +
*Anchors
 +
 +
<div style="clear:both"></div>
==Other Components==
==Other Components==
<div id="horiz-line"></div>
<div id="horiz-line"></div>
-
These parts and a microwave or hotplate are all that is needed to start building with GENOMIKON.
+
These parts and a microwave or hotplate are all that is needed to start building with GENOMIKON.  
 +
(All components are pre-sterilized.)
*Precision Droppers to accurately dispense small volumes of liquids
*Precision Droppers to accurately dispense small volumes of liquids
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*Elution Buffer
*Elution Buffer
*Lyophilized Competent Cells
*Lyophilized Competent Cells
-
*Lyophilized LB media
+
*LB media
*Plates
*Plates
*Iron Micro Beads
*Iron Micro Beads
 +
*Thermometer
 +
[[Image:Alberta_Spread.jpg|550px|center|thumb|All the components of GENOMIKON can be seen in this picture.]]
 +
{{Team:Alberta/endMainContent}}
{{Team:Alberta/endMainContent}}

Latest revision as of 02:31, 28 October 2010

TEAM ALBERTA

The DNA Components

Open Reading Frame Parts

Fluorescent Proteins Carotenoid Pathway Violacein Pathway Transcription Factors Resistance Marker
GFP CrtE VioA LacI Ampicillin
mRFP CrtB VioB AraC Chloramphenicol
CFP CrtI VioD LambdaCI Kanamycin
YFP CrtY VioE TetR Tetramycin
GENOMIKON's DNA parts

Linker Parts

  • Transcription
  • 5 Anderson Promoters
  • Operon Linker
  • Anti-linker as a negative control
  • Short null-linker for inversion of ends
  • Fusion Protein Link
  • Protein Tags


Other Parts

  • High, medium and low copy number Oris
  • Caps
  • Anchors

Other Components

These parts and a microwave or hotplate are all that is needed to start building with GENOMIKON. (All components are pre-sterilized.)

  • Precision Droppers to accurately dispense small volumes of liquids
  • Magnetic Racks
  • Eppendorf Tubes
  • Wash Buffer
  • Elution Buffer
  • Lyophilized Competent Cells
  • LB media
  • Plates
  • Iron Micro Beads
  • Thermometer
All the components of GENOMIKON can be seen in this picture.



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