Team:Calgary/28 June 2010
From 2010.igem.org
(2 intermediate revisions not shown) | |||
Line 1: | Line 1: | ||
{{CalgaryNotebookTemplate| | {{CalgaryNotebookTemplate| | ||
- | + | Monday June 28, 2010| | |
- | + | ||
<u>Jeremy</u> | <u>Jeremy</u> | ||
Line 15: | Line 14: | ||
<u>Patrick</u> | <u>Patrick</u> | ||
- | I ran gels of the ''cpxP'' promoters and the R0040-E0430/R0040-E0420 constructs in 1.5% agarose | + | I ran gels of the ''cpxP'' promoters and the R0040-E0430/R0040-E0420 constructs in 1.5% agarose. |
}} | }} |
Latest revision as of 03:20, 23 August 2010
Notebook
|
||||||||||||||||||||||||||||||||||||||||||||||||||||
|
||||||||||||||||||||||||||||||||||||||||||||||||||||
|
||||||||||||||||||||||||||||||||||||||||||||||||||||
|
||||||||||||||||||||||||||||||||||||||||||||||||||||
|
||||||||||||||||||||||||||||||||||||||||||||||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||
Monday June 28, 2010
Jeremy
Discussion with Thane Kubik. Several changes made to the original project idea including replacing lock key with a more direct approach. No wet lab today.
Chris
Today, we had a discussion with Thane Kubik in which he went over some biological theory with us as well as going through our project with us. He also went through our lab protocols and attempted to troubleshoot as we have been having trouble with our transformations of DNA into competent cells. He gave us advice on changes that should be made to our project circuits as well as going through our basic modelling idea.
Patrick
I ran gels of the cpxP promoters and the R0040-E0430/R0040-E0420 constructs in 1.5% agarose.