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Team:Calgary/23 October 2010
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| - | <u>Emily<u> | + | <u>Emily</u> |
Today I set up a massive colony PCR with the BBK-CP primers in order to veirfy that we were able to get all of our parts into the psB1C3 vector. Today I also made more LB broth. I also did transformations of a few other parts into the psB1C3 vector as well as the construction of I0500-B0034 with the Lethbridge part. | Today I set up a massive colony PCR with the BBK-CP primers in order to veirfy that we were able to get all of our parts into the psB1C3 vector. Today I also made more LB broth. I also did transformations of a few other parts into the psB1C3 vector as well as the construction of I0500-B0034 with the Lethbridge part. | ||
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| + | <u>Himika</u> | ||
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| + | Today I set subcultured the DegP cells containing MalE and MalE31 into 20 ml broth and allowed it to grow for 6 hours. I induced these cells with 15 different arabinose concentrations. This was left shaking overnight and I will read these cells tomorrow. | ||
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Latest revision as of 01:03, 27 October 2010
Saturday October 23, 2010
Emily
Today I set up a massive colony PCR with the BBK-CP primers in order to veirfy that we were able to get all of our parts into the psB1C3 vector. Today I also made more LB broth. I also did transformations of a few other parts into the psB1C3 vector as well as the construction of I0500-B0034 with the Lethbridge part.
Himika
Today I set subcultured the DegP cells containing MalE and MalE31 into 20 ml broth and allowed it to grow for 6 hours. I induced these cells with 15 different arabinose concentrations. This was left shaking overnight and I will read these cells tomorrow.
