Team:Lethbridge/Parts/Characterized Existing Parts
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+ | =<font color="white">Catechol Degradation= | ||
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+ | The focus of our <html><a href="https://2010.igem.org/Team:Lethbridge/Project"><font color="#00DC00">project</font></a></html> is to decrease the toxicity of tailing pond water through bioremediation. We are specifically interested in the <html><a href="https://2010.igem.org/Team:Lethbridge/Project/Catechol_Degradation"><font color="#00DC00">degradation of the toxic molecule catechol</font></a></html> into 2-hydroxymuconate semialdyhyde (2-HMS); a bright yellow substrate that can be metabolized by the cell. This conversion is accomplished by catechol 2,3-dioxygenase (xylE). | ||
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+ | ==<font color="white">Characterized Parts</font>== | ||
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+ | You can view <html><a href="http://partsregistry.org/Part:BBa_K118021" target="new"><font color="#00DC00">BBa_K118021</font></a></html> on the registry and find the experimental data on our <html><a href="https://2010.igem.org/Team:Lethbridge/Results#Placement_of_Oligoarginine_Tail_on_Proteins"><font color="#00DC00">Results</font></a></html> page. | ||
+ | <br><br> | ||
=<font color="white">Compartmentalization Parts= | =<font color="white">Compartmentalization Parts= | ||
- | One of the sub-projects for the bioremediation of the tailings ponds is to create synthetic <html><a href="https://2010.igem.org/Team:Lethbridge/Project/Compartamentalization"><font color="#00DC00"> microcompartments</font></a></html> that we can then use to isolate various | + | One of the sub-projects for the bioremediation of the tailings ponds is to create synthetic <html><a href="https://2010.igem.org/Team:Lethbridge/Project/Compartamentalization"><font color="#00DC00"> microcompartments</font></a></html> that we can then use to isolate various pathways within an <i>Escherichia coli</i> cell. To do this we need to have a microcompartment as well as a means to characterize the compartment so the system can be optimized. These parts we have received directly from the Registry and were further characterized for future use in characterization of the microcompartment system. |
==<font color="white">N-terminal Arginine Fusion Vector== | ==<font color="white">N-terminal Arginine Fusion Vector== | ||
- | You can view <html><a href="http://partsregistry.org/wiki/index.php/Part:BBa_K249004" target="new"><font color="#00DC00">BBa_K249004</font></a></html> on the registry and find the experimental details on our <html><a href="https://2010.igem.org/Team:Lethbridge/Results"><font color="#00DC00">Results</font></a></html> page. | + | You can view <html><a href="http://partsregistry.org/wiki/index.php/Part:BBa_K249004" target="new"><font color="#00DC00">BBa_K249004</font></a></html> on the registry and find the experimental details on our <html><a href="https://2010.igem.org/Team:Lethbridge/Results#Placement_of_Oligoarginine_Tail_on_Proteins"><font color="#00DC00">Results</font></a></html> page. |
==<font color="white">C-terminal Arginine Fusion Vector== | ==<font color="white">C-terminal Arginine Fusion Vector== | ||
- | You can view <html><a href="http://partsregistry.org/wiki/index.php/Part:BBa_K249005"target="new"><font color="#00DC00">BBa_K249005</font></a></html> on the registry and find the experimental details on our <html><a href="https://2010.igem.org/Team:Lethbridge/Results"><font color="#00DC00">Results</font></a></html> page. | + | You can view <html><a href="http://partsregistry.org/wiki/index.php/Part:BBa_K249005"target="new"><font color="#00DC00">BBa_K249005</font></a></html> on the registry and find the experimental details on our <html><a href="https://2010.igem.org/Team:Lethbridge/Results#Placement_of_Oligoarginine_Tail_on_Proteins"><font color="#00DC00">Results</font></a></html> page. |
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+ | =<font color="white">Magnetic Nanoparticles Parts= | ||
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+ | One of the sub-projects for the <html><a href="https://2010.igem.org/Team:Lethbridge/Project" target="new"><font color="#00DC00"> bioremediation of the tailings ponds</font></a></html> is to reduce heavy metals to create <html><a href="https://2010.igem.org/Team:Lethbridge/Project/Magnetic_Nanoparticles" target="new"><font color="#00DC00"> magnetic nanoparticles</font></a></html> that can then be removed from the pond. To do this we need to be able to produce Mms6 (the iron reducing protein) and show that it can successfully produce the nanoparticles within the <i>Escherichia coli</i> cell. He is the work we have accomplished so far to characterize Mms6. | ||
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+ | ==<font color="white">Mms6 (Nanoparticles)== | ||
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+ | You can view <html><a href="http://partsregistry.org/wiki/index.php/Part:BBa_K249019" target="new"><font color="#00DC00">BBa_K249019</font></a></html> on the registry and find the experimental details on our <html><a href="https://2010.igem.org/Team:Lethbridge/Results#Characterization_of_Mms6"><font color="#00DC00">Results</font></a></html> page. | ||
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