Team:IvyTech-South Bend/Notebook

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<a href="https://2010.igem.org/Team:IvyTech-South_Bend"> <img src="https://static.igem.org/mediawiki/2010/f/f3/Home1.png" width=12%></a>   
<a href="https://2010.igem.org/Team:IvyTech-South_Bend"> <img src="https://static.igem.org/mediawiki/2010/f/f3/Home1.png" width=12%></a>   
   
   
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<a href="https://2010.igem.org/Team:IvyTech-South_Bend/Project"> <img src="https://static.igem.org/mediawiki/2010/9/92/Project.png" width=11%></a>     
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<a href="https://2010.igem.org/Team:IvyTech-South_Bend/Project"> <img src="https://static.igem.org/mediawiki/2010/5/58/Image-_SBProject.PNG" width=11%></a>     
<width=80%><a href="https://2010.igem.org/Team:IvyTech-South_Bend/Parts"> <img src="https://static.igem.org/mediawiki/2010/7/79/Parts.png" width=15%></a>     
<width=80%><a href="https://2010.igem.org/Team:IvyTech-South_Bend/Parts"> <img src="https://static.igem.org/mediawiki/2010/7/79/Parts.png" width=15%></a>     
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<a href="https://2010.igem.org/Team:IvyTech-South_Bend/Modeling"> <img src="https://static.igem.org/mediawiki/2010/3/3c/Modeling.png" width=11%></a>
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<a href="https://2010.igem.org/Team:IvyTech-South_Bend/Modeling"> <img src="https://static.igem.org/mediawiki/2010/0/0b/Image-SBModeling.PNG" width=11%></a>
<a href="https://2010.igem.org/Team:IvyTech-South_Bend/Notebook"> <img src="https://static.igem.org/mediawiki/2010/9/95/Notebook.png" width=11%></a>
<a href="https://2010.igem.org/Team:IvyTech-South_Bend/Notebook"> <img src="https://static.igem.org/mediawiki/2010/9/95/Notebook.png" width=11%></a>
<a href="https://2010.igem.org/Team:IvyTech-South_Bend/Safety"> <img src="https://static.igem.org/mediawiki/2010/8/84/Safety.png" width=11%></a>
<a href="https://2010.igem.org/Team:IvyTech-South_Bend/Safety"> <img src="https://static.igem.org/mediawiki/2010/8/84/Safety.png" width=11%></a>
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{|align="justify"
{|align="justify"
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|You can write a background of your team here. Give us a background of your team, the members, etc. Or tell us more about something of your choosing.
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|[[Image:IvyTech-South_Bend_logo.png|200px|right|frame]]
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==Notebook==
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<html>
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<style type="text/css">
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''Tell us more about your project. Give us background. Use this is the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
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table.calendar          { margin: 0; padding: 10px; }
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table.calendar td      { margin: 0; padding: 2px; vertical-align: top; }
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table.month .heading td { padding:2px; background-color:#0f6666; color:#00ff00; text-align:center; font-size:120%; font-weight:bold; }
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table.month .dow td    { color:#00ff00; text-align:center; font-size:110%; }
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table.month td.today    { background-color:#ddd;}
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table.month td {
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    border: none;
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    margin: 0;
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    padding: 1.5pt 2pt;
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    font-weight: bold;
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    font-size: 10pt;
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    text-align: center;
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    background-color: #000000;
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    }
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#bodyContent table.month a { background:none; padding:0 }
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.day-active { color:#00FF00 }
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.day-empty  { color:#006666 }
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</style>
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</html>
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{| cellpadding="20" align="center"
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|{{ #calendar: title=Talk:Team:IvyTech-South_Bend |year=2010 | month=06 }}
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|{{ #calendar: title=Talk:Team:IvyTech-South_Bend |year=2010 | month=07 }}
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|{{ #calendar: title=Talk:Team:IvyTech-South_Bend |year=2010 | month=08 }}
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|{{ #calendar: title=Talk:Team:IvyTech-South_Bend |year=2010 | month=09 }}
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|{{ #calendar: title=Talk:Team:IvyTech-South_Bend |year=2010 | month=10 }}
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|{{ #calendar: title=Talk:Team:IvyTech-South_Bend |year=2010 | month=11 }}
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|}
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===IGEM Part #s and Uses===
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BBa_T9002 – Producer Controler Device–Plate2 Well 9A
 +
 
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BBa_I732017 – LacZ with our GFP 1—Plate 2 Well 3K
 +
 
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BBa_F2620– LuxR with terminator– Plate 2 Well 6E
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BBa_I13522 –Tet Prop with GFP—Plate 2 Well 8A
 +
 
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BBa_T13521 Red Flouro tet RFP—Plate 2 Well 6O
 +
 
 +
BBa_pSB1C3—Plasmid—Plate 1 Well 3A
 +
 
 +
BBa_pSB1T3—Tetracycline resistant plasmid—Plate 1 Well 7A
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BBa_pSB1AT3--high copy number plasmid carrying ampicillin and tetracycline resistance--Plate 1 Well 13A
 +
 
 +
== 6/28/10 ==
 +
Preparing SOB
 +
 
 +
== 6/30/10 ==
 +
 
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Richard Lab:Electroporation of E. coli
 +
 
 +
== 7/28/10 ==
 +
 
 +
Usage and extraction
 +
 
 +
== 8/24/10 ==
 +
 
 +
Today we will be pouring new plates for streaking new transformed cells.
 +
 
 +
== 8/27/10 ==
 +
 
 +
 
 +
We will be running a gel to determine if our DNA sample was successfully electroplated.
 +
 
 +
== 8/27/10 ==
 +
 
 +
Today we will be electroplating part KBBa_3131010
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 +
== 8/31/10 ==
 +
 
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Results of transformation - the plates had growth but (no) distinct colony pattern.
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== 8/31/10 ==
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 +
After agar is dissolved, and allowed to cool, I will add Amp in different doses to specific plates. JH
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 +
== 9/1/10 ==
 +
 
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Today the team will be making up the same plates as I made yesterday. Professor T. wants new Amp stock made to document how much is used into the stock.
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 +
== 9/2/10 ==
 +
 
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Results from streaking –
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 +
== 9/2/10 ==
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Protocol For Making LB-Broth/Agar
 +
 
 +
== 9/3/10 ==
 +
 
 +
Today I’m finishing making the LB/Agar from yesterday.
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 +
== 9/9/10 ==
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 +
Due to conflict we will be changing from E Coli to yeast.
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 +
== 9/10/10 ==
 +
 
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Lux Casette Right Promoter BBa_I1051
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 +
== 9/14/10 ==
 +
 
 +
Today we found growth from our Electroporated E – Coli parts
 +
 
 +
== 9/15/10 ==
 +
 
 +
Today we will be extracting DNA from out electroporated cells/T9002
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B-galactosidase Assay
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 +
== 9/16/10 ==
 +
 
 +
Today we will wash
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== 9/17/10 ==
 +
 
 +
Today we will be doing a DNA Extraction from/of part T9002 for electroporation this will determine if we have a stable host for our beast this will also Work!
 +
 
 +
 
 +
== 10/18/10 ==
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cutting enzymes
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== 9/21/10 ==
 +
 
 +
Today we will electroporate Agrobacterium with part T9002
 +
 
 +
Electrophoresis
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 +
== 9/22/10 ==
 +
 
 +
Today we have growth from electroporation done yesterday we are going to test by hitting with (AHL) the 20 mL grew not 5 mL sample
 +
 
 +
== 9/22/10 ==
 +
 
 +
The only one that should glow should be (C)/(3) E – Coli w/transformed part in LB For Supernate of A.Tumafaciens
 +
 
 +
== 9/23/10 ==
 +
 
 +
Today I have been setting up for the trial of our part T9002 inside agrobacteria to see if in the presence of AHL it floureces.
 +
 
 +
==9/24/10 ==
 +
 
 +
Today I’m testing absorbance of our trials @ 395nm
 +
 
 +
== 9/28/10 ==
 +
 
 +
Today we will be picking 2 colonies from the Lacz, LuxR electroporated plates that were streaked on 9/22/10
 +
 
 +
== 9/30/10==
 +
LacZ without GFP also with RBS and terminator
 +
 
 +
 
 +
==10/1/10==
 +
 
 +
electrophoresis
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 +
==10/5/10==
 +
 
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pTet GFP
 +
 
 +
==10/7/10==
 +
 
 +
Electroporating 2 parts with E-coli bacteria
 +
 
 +
==10/8/10==
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 +
 
 +
LB-Lennox Broth & Electroporation
 +
 
 +
==10/12/10==
 +
hold until Friday
 +
 
 +
==10/13/10==
 +
Discoveries!
 +
 
 +
==10/15/10==
 +
 
 +
Using openwetware.org protocol
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B-galactosidase Assay
 +
 
 +
BBL Trypticase soy Broth and Agar
 +
 
 +
DPBS
 +
 
 +
== 10/19/10==
 +
Protocol-- Electroporation man for E-Coli
 +
== 10/21/10 ==
 +
High Efficiency Electrotransformation of E.coli
 +
 
 +
== 10/25/10 ==
 +
 
 +
DNA from BlueGuy
 +
 
 +
== 10/26/10 ==
 +
Final steps pack and ship
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 +
 
|[[Image:IvyTech-South_Bend_team.png|right|frame|Your team picture]]
|[[Image:IvyTech-South_Bend_team.png|right|frame|Your team picture]]
|-
|-
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|align="center"|[[Image:IvyTech-South_Bend_logo.png|200px|right|frame]]
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|align="center"|[[Team:IvyTech-South_Bend | Team Example]]
 
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!align="center"|[[Team:IvyTech-South_Bend/Safety|Safety]]
!align="center"|[[Team:IvyTech-South_Bend/Safety|Safety]]
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==Notebook==
 
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You should make use of the calendar feature on the wiki and start a lab notebook.  This may be looked at by the judges to see how your work progressed throughout the summer.  It is a very useful organizational tool as well.
 

Latest revision as of 00:44, 28 October 2010

Discussion

Contents

Notebook

June
MTWTFSS
  [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/1_June_2010&action=edit 1] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/2_June_2010&action=edit 2] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/3_June_2010&action=edit 3] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/4_June_2010&action=edit 4] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/5_June_2010&action=edit 5] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/6_June_2010&action=edit 6]
[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/7_June_2010&action=edit 7] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/8_June_2010&action=edit 8] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/9_June_2010&action=edit 9] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/10_June_2010&action=edit 10] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/11_June_2010&action=edit 11] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/12_June_2010&action=edit 12] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/13_June_2010&action=edit 13]
[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/14_June_2010&action=edit 14] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/15_June_2010&action=edit 15] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/16_June_2010&action=edit 16] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/17_June_2010&action=edit 17] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/18_June_2010&action=edit 18] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/19_June_2010&action=edit 19] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/20_June_2010&action=edit 20]
[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/21_June_2010&action=edit 21] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/22_June_2010&action=edit 22] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/23_June_2010&action=edit 23] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/24_June_2010&action=edit 24] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/25_June_2010&action=edit 25] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/26_June_2010&action=edit 26] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/27_June_2010&action=edit 27]
[http://2010.igem.org/Talk:Team:IvyTech-South_Bend/28_June_2010 28] [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/29_June_2010 29] [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/30_June_2010 30]
July
MTWTFSS
      [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/1_July_2010 1] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/2_July_2010&action=edit 2] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/3_July_2010&action=edit 3] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/4_July_2010&action=edit 4]
[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/5_July_2010&action=edit 5] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/6_July_2010&action=edit 6] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/7_July_2010&action=edit 7] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/8_July_2010&action=edit 8] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/9_July_2010&action=edit 9] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/10_July_2010&action=edit 10] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/11_July_2010&action=edit 11]
[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/12_July_2010&action=edit 12] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/13_July_2010&action=edit 13] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/14_July_2010&action=edit 14] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/15_July_2010&action=edit 15] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/16_July_2010&action=edit 16] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/17_July_2010&action=edit 17] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/18_July_2010&action=edit 18]
[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/19_July_2010&action=edit 19] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/20_July_2010&action=edit 20] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/21_July_2010&action=edit 21] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/22_July_2010&action=edit 22] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/23_July_2010&action=edit 23] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/24_July_2010&action=edit 24] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/25_July_2010&action=edit 25]
[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/26_July_2010&action=edit 26] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/27_July_2010&action=edit 27] [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/28_July_2010 28] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/29_July_2010&action=edit 29] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/30_July_2010&action=edit 30] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/31_July_2010&action=edit 31]
August
MTWTFSS
            [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/1_August_2010&action=edit 1]
[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/2_August_2010&action=edit 2] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/3_August_2010&action=edit 3] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/4_August_2010&action=edit 4] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/5_August_2010&action=edit 5] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/6_August_2010&action=edit 6] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/7_August_2010&action=edit 7] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/8_August_2010&action=edit 8]
[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/9_August_2010&action=edit 9] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/10_August_2010&action=edit 10] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/11_August_2010&action=edit 11] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/12_August_2010&action=edit 12] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/13_August_2010&action=edit 13] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/14_August_2010&action=edit 14] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/15_August_2010&action=edit 15]
[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/16_August_2010&action=edit 16] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/17_August_2010&action=edit 17] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/18_August_2010&action=edit 18] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/19_August_2010&action=edit 19] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/20_August_2010&action=edit 20] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/21_August_2010&action=edit 21] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/22_August_2010&action=edit 22]
[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/23_August_2010&action=edit 23] [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/24_August_2010 24] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/25_August_2010&action=edit 25] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/26_August_2010&action=edit 26] [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/27_August_2010 27] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/28_August_2010&action=edit 28] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/29_August_2010&action=edit 29]
[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/30_August_2010&action=edit 30] [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/31_August_2010 31]
September
MTWTFSS
    [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/1_September_2010 1] [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/2_September_2010 2] [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/3_September_2010 3] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/4_September_2010&action=edit 4] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/5_September_2010&action=edit 5]
[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/6_September_2010&action=edit 6] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/7_September_2010&action=edit 7] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/8_September_2010&action=edit 8] [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/9_September_2010 9] [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/10_September_2010 10] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/11_September_2010&action=edit 11] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/12_September_2010&action=edit 12]
[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/13_September_2010&action=edit 13] [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/14_September_2010 14] [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/15_September_2010 15] [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/16_September_2010 16] [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/17_September_2010 17] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/18_September_2010&action=edit 18] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/19_September_2010&action=edit 19]
[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/20_September_2010&action=edit 20] [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/21_September_2010 21] [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/22_September_2010 22] [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/23_September_2010 23] [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/24_September_2010 24] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/25_September_2010&action=edit 25] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/26_September_2010&action=edit 26]
[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/27_September_2010&action=edit 27] [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/28_September_2010 28] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/29_September_2010&action=edit 29] [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/30_September_2010 30]
October
MTWTFSS
        [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/1_October_2010 1] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/2_October_2010&action=edit 2] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/3_October_2010&action=edit 3]
[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/4_October_2010&action=edit 4] [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/5_October_2010 5] [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/6_October_2010 6] [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/7_October_2010 7] [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/8_October_2010 8] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/9_October_2010&action=edit 9] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/10_October_2010&action=edit 10]
[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/11_October_2010&action=edit 11] [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/12_October_2010 12] [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/13_October_2010 13] [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/14_October_2010 14] [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/15_October_2010 15] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/16_October_2010&action=edit 16] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/17_October_2010&action=edit 17]
[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/18_October_2010&action=edit 18] [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/19_October_2010 19] [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/20_October_2010 20] [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/21_October_2010 21] [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/22_October_2010 22] [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/23_October_2010 23] [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/24_October_2010 24]
[http://2010.igem.org/Talk:Team:IvyTech-South_Bend/25_October_2010 25] [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/26_October_2010 26] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/27_October_2010&action=edit 27] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/28_October_2010&action=edit 28] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/29_October_2010&action=edit 29] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/30_October_2010&action=edit 30] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/31_October_2010&action=edit 31]
November
MTWTFSS
[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/1_November_2010&action=edit 1] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/2_November_2010&action=edit 2] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/3_November_2010&action=edit 3] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/4_November_2010&action=edit 4] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/5_November_2010&action=edit 5] [http://2010.igem.org/Talk:Team:IvyTech-South_Bend/6_November_2010 6] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/7_November_2010&action=edit 7]
[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/8_November_2010&action=edit 8] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/9_November_2010&action=edit 9] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/10_November_2010&action=edit 10] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/11_November_2010&action=edit 11] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/12_November_2010&action=edit 12] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/13_November_2010&action=edit 13] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/14_November_2010&action=edit 14]
[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/15_November_2010&action=edit 15] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/16_November_2010&action=edit 16] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/17_November_2010&action=edit 17] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/18_November_2010&action=edit 18] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/19_November_2010&action=edit 19] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/20_November_2010&action=edit 20] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/21_November_2010&action=edit 21]
[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/22_November_2010&action=edit 22] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/23_November_2010&action=edit 23] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/24_November_2010&action=edit 24] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/25_November_2010&action=edit 25] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/26_November_2010&action=edit 26] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/27_November_2010&action=edit 27] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/28_November_2010&action=edit 28]
[http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/29_November_2010&action=edit 29] [http://2010.igem.org/wiki/index.php?title=Talk:Team:IvyTech-South_Bend/30_November_2010&action=edit 30]

IGEM Part #s and Uses

BBa_T9002 – Producer Controler Device–Plate2 Well 9A

BBa_I732017 – LacZ with our GFP 1—Plate 2 Well 3K

BBa_F2620– LuxR with terminator– Plate 2 Well 6E

BBa_I13522 –Tet Prop with GFP—Plate 2 Well 8A

BBa_T13521 Red Flouro tet RFP—Plate 2 Well 6O

BBa_pSB1C3—Plasmid—Plate 1 Well 3A

BBa_pSB1T3—Tetracycline resistant plasmid—Plate 1 Well 7A

BBa_pSB1AT3--high copy number plasmid carrying ampicillin and tetracycline resistance--Plate 1 Well 13A

6/28/10

Preparing SOB

6/30/10

Richard Lab:Electroporation of E. coli

7/28/10

Usage and extraction

8/24/10

Today we will be pouring new plates for streaking new transformed cells.

8/27/10

We will be running a gel to determine if our DNA sample was successfully electroplated.

8/27/10

Today we will be electroplating part KBBa_3131010

8/31/10

Results of transformation - the plates had growth but (no) distinct colony pattern.

8/31/10

After agar is dissolved, and allowed to cool, I will add Amp in different doses to specific plates. JH

9/1/10

Today the team will be making up the same plates as I made yesterday. Professor T. wants new Amp stock made to document how much is used into the stock.

9/2/10

Results from streaking –

9/2/10

Protocol For Making LB-Broth/Agar

9/3/10

Today I’m finishing making the LB/Agar from yesterday.

9/9/10

Due to conflict we will be changing from E Coli to yeast.

9/10/10

Lux Casette Right Promoter BBa_I1051

9/14/10

Today we found growth from our Electroporated E – Coli parts

9/15/10

Today we will be extracting DNA from out electroporated cells/T9002 B-galactosidase Assay

9/16/10

Today we will wash

9/17/10

Today we will be doing a DNA Extraction from/of part T9002 for electroporation this will determine if we have a stable host for our beast this will also Work!


10/18/10

cutting enzymes

9/21/10

Today we will electroporate Agrobacterium with part T9002

Electrophoresis

9/22/10

Today we have growth from electroporation done yesterday we are going to test by hitting with (AHL) the 20 mL grew not 5 mL sample

9/22/10

The only one that should glow should be (C)/(3) E – Coli w/transformed part in LB For Supernate of A.Tumafaciens

9/23/10

Today I have been setting up for the trial of our part T9002 inside agrobacteria to see if in the presence of AHL it floureces.

9/24/10

Today I’m testing absorbance of our trials @ 395nm

9/28/10

Today we will be picking 2 colonies from the Lacz, LuxR electroporated plates that were streaked on 9/22/10

9/30/10

LacZ without GFP also with RBS and terminator


10/1/10

electrophoresis

10/5/10

pTet GFP

10/7/10

Electroporating 2 parts with E-coli bacteria

10/8/10

LB-Lennox Broth & Electroporation

10/12/10

hold until Friday

10/13/10

Discoveries!

10/15/10

Using openwetware.org protocol

B-galactosidase Assay

BBL Trypticase soy Broth and Agar

DPBS

10/19/10

Protocol-- Electroporation man for E-Coli

10/21/10

High Efficiency Electrotransformation of E.coli

10/25/10

DNA from BlueGuy

10/26/10

Final steps pack and ship


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