Team:TU Delft/17 June 2010 content

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===More media===
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=More media=
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[[Image:TU_Delft_DSC4845.jpg|300px|thumb|right]]
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Today camera crew #2 came by from Nanopodium, a platform for exchanging thoughts, ideas, opinions and best practices on nanotechnology. They are making a movie about our project for the Nano Video Hunt.  This is a campaign started with Hyves were young people are challenged to post videos about their views on nanotechnology.
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Today camera crew #2 came by from Nanopodium.
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For more information look at the following websites:
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[http://www.nanovideohunt.nl www.nanovideohunt.nl]
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[http://www.nanovideohunt.hyves.nl www.nanovideohunt.hyves.nl]
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[http://www.nanopodium.nl www.nanopodium.nl]
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[[Image:TU_Delft_DSC4845.jpg|325px|thumb|left]]
[[Image:TU_Delft_DSC4848.JPG|325px|thumb|left]]
[[Image:TU_Delft_DSC4848.JPG|325px|thumb|left]]
[[Image:TU_Delft_DSC4850.JPG|325px|thumb|left]]
[[Image:TU_Delft_DSC4850.JPG|325px|thumb|left]]
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<html><div></div></html>
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<html><div></div></html>
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==Lab work==
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By Hugo
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I made some time for doing some transformations from the Biobrick plates, I will give you the list of the biobricks that I tried to transform.
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{| style="color:black; background-color:white;" cellpadding="4" cellspacing="0" border="1"
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|'''#'''
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|'''Code'''
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|'''Resistance marker'''
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|'''Function'''
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|-
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|1
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|J61100
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|Amp
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|RBS Anderson family
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|-
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|2
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|J61101
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|Amp
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|RBS Anderson family
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|-
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|3
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|J61107
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|Amp
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|RBS Anderson family
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|-
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|4
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|J61117
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|Amp
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|RBS Anderson family
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|-
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|5
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|J61127
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|Amp
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|RBS Anderson family
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|-
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|6
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|B0034
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|Amp
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|RBS 
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|-
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|7
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|J23109
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|Amp
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|Promoter Anderson family
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|-
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|8
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|B0032
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|Amp
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|RBS Anderson family
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|-
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|9
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|R0011
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|Amp
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|LacI promoter
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|-
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|10
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|B0015
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|Amp, Kan
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|Double terminator
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|-
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|11
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|R0010
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|Amp
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|LacI
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|-
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|12
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|E0422
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|Amp
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|YFP generator
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|-
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|13
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|E0240
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|Amp
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|GFP generator
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|-
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|14
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|E0040
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|Amp
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|GFP
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|-
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|15
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|I13401
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|Amp
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|GFP + Double terminator
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|}
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Procedure:
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* Add 1 ul of DNA to 33 ul of competent cells
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* Put the tubes on ice for 10 min
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* Warm the tubes in a water bath at 37º C for 5 min.
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* Add 250 ul of LB medium
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* Incubate the cells 1 hour at 37ºC, shake at 160 rpm
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* Plate 200 ul over selective medium

Latest revision as of 16:01, 25 August 2010

More media

Today camera crew #2 came by from Nanopodium, a platform for exchanging thoughts, ideas, opinions and best practices on nanotechnology. They are making a movie about our project for the Nano Video Hunt. This is a campaign started with Hyves were young people are challenged to post videos about their views on nanotechnology.

For more information look at the following websites:

[http://www.nanovideohunt.nl www.nanovideohunt.nl]

[http://www.nanovideohunt.hyves.nl www.nanovideohunt.hyves.nl]

[http://www.nanopodium.nl www.nanopodium.nl]

TU Delft DSC4845.jpg
TU Delft DSC4848.JPG
TU Delft DSC4850.JPG




































Lab work

By Hugo

I made some time for doing some transformations from the Biobrick plates, I will give you the list of the biobricks that I tried to transform.

# Code Resistance marker Function
1 J61100 Amp RBS Anderson family
2 J61101 Amp RBS Anderson family
3 J61107 Amp RBS Anderson family
4 J61117 Amp RBS Anderson family
5 J61127 Amp RBS Anderson family
6 B0034 Amp RBS
7 J23109 Amp Promoter Anderson family
8 B0032 Amp RBS Anderson family
9 R0011 Amp LacI promoter
10 B0015 Amp, Kan Double terminator
11 R0010 Amp LacI
12 E0422 Amp YFP generator
13 E0240 Amp GFP generator
14 E0040 Amp GFP
15 I13401 Amp GFP + Double terminator


Procedure:

  • Add 1 ul of DNA to 33 ul of competent cells
  • Put the tubes on ice for 10 min
  • Warm the tubes in a water bath at 37º C for 5 min.
  • Add 250 ul of LB medium
  • Incubate the cells 1 hour at 37ºC, shake at 160 rpm
  • Plate 200 ul over selective medium
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