Team:Caltech/Protocols

From 2010.igem.org

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__NOTOC__
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* Making V1012 competent
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* [[Team:Caltech/Competent Cells|Competent Cells]]
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* Making stock solutions
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* [[Team:Caltech/Electroporation|Transformation by Electroporation]]
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* Freezer (glycerol) stocks
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* [[Team:Caltech/Glycerol Stocks|Freezer (glycerol) stocks]]
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* Plating antibiotics
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* [[Team:Caltech/PlatingAntibiotics|Plating antibiotics]]
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* Sequencing primers
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* [[Team:Caltech/SeqPrimers|Sequencing primers]]
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* Making synthase casette
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<!--* [[Team:Caltech/MakingPlastic|Growing cells with PHA (and substrate oil)]]-->
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** Sources of sequences
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* [[Team:Caltech/Colony PCR|Colony PCR]]
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** Optimizing & checking genes
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* [[Team:Caltech/Cloning|Two-day Cloning]]
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* Chemical/Crosslinking reactions
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** Digesting substrate (hydrolysis) + epoxidation (MCPBA)
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** crosslinking (AIBN3) + UV initiation
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* Inducing lysis via 632nm light initiation
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* Making cell/glycerol/agar mixture
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** Applying this to plates
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}}
}}

Latest revision as of 23:20, 24 October 2010


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