"
Team:UPO-Sevilla/Notebook/08 09
From 2010.igem.org
| (2 intermediate revisions not shown) | |||
| Line 20: | Line 20: | ||
<h2>Assembly Team</h2> | <h2>Assembly Team</h2> | ||
| - | <p>We analyzed plaques spread the day before; it looked like if the bacteria could not grow. There were only three red colonies (negatives) in a plaque. Those devices had promoter and were assembled in high copy vectors. This is why we thought that the high expression of the devices could be harmful for bacteria. We analyzed the digestions in agarose gel to verify it. Almost all the digestions were well made and we only had to repeat two. In order to avoid that fact again, we assembled devices including promoters in low copy vectors. Like in before cases, we used different vectors depending | + | <p>We analyzed plaques spread the day before; it looked like if the bacteria could not grow. There were only three red colonies (negatives) in a plaque. Those devices had promoter and were assembled in high copy vectors. This is why we thought that the high expression of the devices could be harmful for bacteria. We analyzed the digestions in agarose gel to verify it. Almost all the digestions were well made and we only had to repeat two. In order to avoid that fact again, we assembled devices including promoters in low copy vectors. Like in before cases, we used different vectors depending on the resistance of the origin vectors we need. Below it is summed up:</p> |
<ul> | <ul> | ||
| Line 32: | Line 32: | ||
</ul> | </ul> | ||
| - | <p> | + | <p>The necessary digestions were made and the ligations were left over night at 13ºC on average.</p> |
| - | <p> | + | <p>In other hand, digestion: 2+5+3 with BamI-PstI. We started the following devices (3A method): 7+2+28; 2+6+2+5+3; 2+18; 28+3; 2+4+2+6; 2+28; 18+3: digestion, ligation and transformation.</p> |
<h2>DryLab Team</h2> | <h2>DryLab Team</h2> | ||
Latest revision as of 19:59, 27 October 2010
August, 9th
Assembly Team
We analyzed plaques spread the day before; it looked like if the bacteria could not grow. There were only three red colonies (negatives) in a plaque. Those devices had promoter and were assembled in high copy vectors. This is why we thought that the high expression of the devices could be harmful for bacteria. We analyzed the digestions in agarose gel to verify it. Almost all the digestions were well made and we only had to repeat two. In order to avoid that fact again, we assembled devices including promoters in low copy vectors. Like in before cases, we used different vectors depending on the resistance of the origin vectors we need. Below it is summed up:
- pSB4K5: (1+2) + (4)
- pSB4C5: (1+2) + (7+2)
- pSB3T5: (11) + (19) // (12) + (19)
- pSB3C5: (11+2) + (13+3) // (12+2) + (13+3) // (11+2) + (16+3) // (12+2) + (16+3) // (1+2) + (16+3)
The necessary digestions were made and the ligations were left over night at 13ºC on average.
In other hand, digestion: 2+5+3 with BamI-PstI. We started the following devices (3A method): 7+2+28; 2+6+2+5+3; 2+18; 28+3; 2+4+2+6; 2+28; 18+3: digestion, ligation and transformation.
DryLab Team
Modeling:
Simulation issues concerning concentrations' stability fixed. Concentrations behave as expected.
Beginning of E-coli movement modeling, basing on tumbling and straight swim.
First implementation of a random movement is successful.
Simulation of bacterial movement in an environment with Chemoattractants started. Bacteria move towards the concentration gradient, but they tend to move futher away once they reach the highest concentration point. Besides, when bacteria approach a side of the recipient they are in, they usually experience troubles coming back.
By the end of the day, the aforementioned issues are all fixed.
Go to 6th of August Go to 10th of August Return to Notebook
Sponsors
