Latest revision as of 00:33, 28 October 2010

Transformation of ligated yneA into pGFPrrnB and pSB1C3

To produce more colonies of the plasmid pGFPrrnB and pSB1C3 containing yneA with pGFPrrnB and pSB1C3 fromyesterday.

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-=Miniprep for ''yneA'', pGFPrrnB and pSB1C3=
-==Aim==
-The aim of this experiment is to produce stocks of ''yneA'', pGFPrrnB and pSB1C3.
-==Materials and Protocol==
-Please refer to [[Team:Newcastle/Qiagen_Minipreps|miniprep]] and [[TeamNewcastleNanoDrop_Spectrophotometer|nanodrop]].
-==Results==
-{|border=1
-|-
-! Sample no.
-! ''yneA''
-! pGFPrrnB
-! pSB1C3
-|-
-!1
-!432.5
-!238.9
-!126.1
-|-
-!2
-!347.6
-!230.7
-!107.6
-|-
-!3
-!380.2
-!390.9
-!121.5
-|-
-!4
-!377.9
-!236.2
-!112.8
-|}
-'''Table 1''': Nanodrop spectrophotometer results. Table represents the amount of plasmid present in µl/ml quantity.
-===Discussion===
-Results from the NanoDrop show concentration values of more than 100 ng/µl, which means we have obtained good concentration of DNA from the miniprep.
-===Conclusion===
-We keep the miniprep products as stocks for future use.
 =Transformation of ligated ''yneA'' into pGFPrrnB and pSB1C3=
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 Please refer to [[Team:Newcastle/Transformation_of_E._coli|transformation of E. coli]].
-==Plating ''Bacillus subtilis'' BFS678==
-Has pMutin4 (and thus ''lacI'') integrated into the chromosome...will be useful for characterisation.. will be transforming this strain with our filamentous cell and arginase BioBricks.
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