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Team:Newcastle/Meetings/1 April 2010
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| - | + | ==Formal Meeting - 1st April 2010== | |
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| - | + | *Room: CBCB; Start: 4 p.m. | |
| - | + | *Chair: Da, Minutes: Alan, Computer: Younus | |
| - | + | *[[Team:Newcastle/Agendas/1_April_2010|Agenda]] | |
| - | + | ||
| - | | | + | ====Roll call and apologies==== |
| - | + | *Present: Jen, Janetta, Steven, Younus, Da, Alan, Richard, Rachel, Jem, Matt | |
| - | + | *Apologies: Phil, Harsh and Zoltan (Holiday); Neil | |
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| - | + | ====Approval of agenda and minutes==== | |
| - | + | *Approved | |
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| - | + | ====Research Feedback==== | |
| - | + | ||
| - | + | =====Nutrient===== | |
| - | + | *Using a glucose and starch concentration gradient. Uses the catabolic repression system to allow glue production. *Have to test and find suitable starch and chemoattractant!!!!!!!! | |
| - | + | ||
| - | + | =====Sporulation, germination and secretion control===== | |
| - | + | *AbrB gene maybe a suitable gene to repress sporulation by overexpressing it. | |
| - | + | *Can also activate antimicrobial peptides like TasA. | |
| - | + | *Need to find what other genes that are involved in the pathway of AbrB. | |
| - | + | ||
| - | + | =====Quorum Sensing===== | |
| - | Using a glucose and starch concentration gradient. Uses the catabolic repression system to allow glue production. Have to test and find suitable starch and chemoattractant!!!!!!!! | + | *Number of competence cells not an issue. Sin operone to control a lot of pathway. |
| - | + | ||
| - | + | =====Other points===== | |
| - | AbrB gene maybe a suitable gene to repress sporulation by overexpressing it. Can also activate antimicrobial peptides like TasA. | + | #FtsZ knockout to allow filamentous growth, thereby forming a mesh with glue either secreted out or remains in the cells until lysis. |
| - | + | #Is spider silk able to resist the alkali conditions? | |
| - | + | #Is it possible to produce spider silk in ''Bacillus''? | |
| - | Number of competence cells not an issue. Sin operone to control a lot of pathway. | + | |
| - | + | ====Voigt paper feedback==== | |
| - | + | *Salmonella to secrete spider silk contained in SPI through T3SS. SPI to contain ADF1, ADF2 and ADF3 genes. | |
| - | + | *Does ''Bacillus'' have a T3SS? Can we allow proteins to fold in the cells then induce lysis? To check out ''Bacillus'' secretion system. | |
| - | + | ||
| - | + | ====Travel Plans==== | |
| - | + | *Too early to book flights and hotels. May have other members joining. | |
| - | + | *Visa/Passport update: Harsh to apply on May, June period | |
| - | Salmonella to secrete spider silk contained in SPI through T3SS. SPI to contain ADF1, ADF2 and ADF3 genes. Does Bacillus have a T3SS? Can we allow proteins to fold in the cells then induce lysis? To check out Bacillus secretion system. | + | |
| - | + | ====Tutorial==== | |
| - | + | Next tutorial to be on the wiki after | |
| - | + | ||
| - | + | ====Flowchart==== | |
| - | + | *[[Media:Flowchart_overview-1-.ppt|Flow Chart]] | |
| - | Too early to book flights and hotels. May have other members joining. | + | *Need to be more in depth. i.e. The problems, what the bacteria need to do to achieve the goal and the genes that are needed and why. |
| - | + | *Another flow chart to show the different stituation and how the bacteria response to it. | |
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| - | Next tutorial to be on the | + | ====Other business==== |
| - | + | *Foxit not working | |
| - | + | *Sponsors on T-shirt: contact sponsors next week (Jannetta) | |
| - | [[Media:Flowchart_overview-1-.ppt ]] | + | |
| - | + | ==== Action points==== | |
| - | + | # To draft email to Colin and Chris and show it to instructors first(everyone) | |
| - | + | # To continue to develop flow chart(everyone) | |
| - | + | # Tutorial on Wiki | |
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| - | + | ====Next Meeting ==== | |
| - | + | *Informal meeting: 070410 at 11 a.m. in Walton library | |
| - | + | * Formal meeting: 070410 at 3 p.m. in CBCB | |
| - | + | *Chair: Richard, Minutes: Rachel, Computer: Jannetta | |
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Latest revision as of 09:29, 27 October 2010
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Contents |
Formal Meeting - 1st April 2010
- Room: CBCB; Start: 4 p.m.
- Chair: Da, Minutes: Alan, Computer: Younus
- Agenda
Roll call and apologies
- Present: Jen, Janetta, Steven, Younus, Da, Alan, Richard, Rachel, Jem, Matt
- Apologies: Phil, Harsh and Zoltan (Holiday); Neil
Approval of agenda and minutes
- Approved
Research Feedback
Nutrient
- Using a glucose and starch concentration gradient. Uses the catabolic repression system to allow glue production. *Have to test and find suitable starch and chemoattractant!!!!!!!!
Sporulation, germination and secretion control
- AbrB gene maybe a suitable gene to repress sporulation by overexpressing it.
- Can also activate antimicrobial peptides like TasA.
- Need to find what other genes that are involved in the pathway of AbrB.
Quorum Sensing
- Number of competence cells not an issue. Sin operone to control a lot of pathway.
Other points
- FtsZ knockout to allow filamentous growth, thereby forming a mesh with glue either secreted out or remains in the cells until lysis.
- Is spider silk able to resist the alkali conditions?
- Is it possible to produce spider silk in Bacillus?
Voigt paper feedback
- Salmonella to secrete spider silk contained in SPI through T3SS. SPI to contain ADF1, ADF2 and ADF3 genes.
- Does Bacillus have a T3SS? Can we allow proteins to fold in the cells then induce lysis? To check out Bacillus secretion system.
Travel Plans
- Too early to book flights and hotels. May have other members joining.
- Visa/Passport update: Harsh to apply on May, June period
Tutorial
Next tutorial to be on the wiki after
Flowchart
- Flow Chart
- Need to be more in depth. i.e. The problems, what the bacteria need to do to achieve the goal and the genes that are needed and why.
- Another flow chart to show the different stituation and how the bacteria response to it.
Other business
- Foxit not working
- Sponsors on T-shirt: contact sponsors next week (Jannetta)
Action points
- To draft email to Colin and Chris and show it to instructors first(everyone)
- To continue to develop flow chart(everyone)
- Tutorial on Wiki
Next Meeting
- Informal meeting: 070410 at 11 a.m. in Walton library
- Formal meeting: 070410 at 3 p.m. in CBCB
- Chair: Richard, Minutes: Rachel, Computer: Jannetta
