Team:Newcastle/3 September 2010

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{{Team:Newcastle/mainbanner}}
{{Team:Newcastle/mainbanner}}
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=''yneA''=
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=Levan glue=
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==Aims==
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We started working on levan. For this we used agar plates containing 10% sucrose and ''Bacillus subtilis'' 168 was grown onto that medium. The sucrose cultures and controls were compared. The cultures grown on sucrose should be considerably thicker.
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The aim of the experiment is to test for the correct integration of ''yne''A in pGFPrrnb plasmid.
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The overnight cultures of the different strains were also grown on solid agar plates with and without sucrose.
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For more information levan, please refer to: [[Team:Newcastle/glue|levan]].
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==Materials and protocols==
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[[Image:DSC01022.JPG|500px|''B. subtilis producing levan]]
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#Please refer to: [[Team:Newcastle/Qiagen_Minipreps#Plasmid_extraction| Plasmid extraction]].
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'''Image 1''': Image showing ''B. subtilis'' producing levan on agar plate containing 10% sucrose.
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#Please refer to:[[Team:Newcastle/Restriction_digests|Restriction digest]]. We used Nhe1 and Spe1 to remove the ''yneA'' from pGFP-rrnB.
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#Please refer to:[[Team:Newcastle/Gel_electrophoresis| Gel electrophoresis]] for running all the digested plasmid fragments.
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==Results==
 
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[[Image:03.09.10.png#file|400px]]
 
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'''Figure 1''':
 
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* '''Lane 1''': 1kb DNA ladder
 
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* '''Lane 2''': Plamid pMutin4 containing ''lacI''
 
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* '''Lane 3''': Plamid pMutin4 containing Pspac_oid promoter
 
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* '''Lane 4''': 100bp DNA ladder
 
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* '''Lane 5''':
 
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* '''Lane 6''':
 
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* '''Lane 7''':
 
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* '''Lane 8''':
 
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* '''Lane 9''':
 
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* '''Lane 10''':
 
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* '''Lane 11''':
 
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* '''Lane 12''':
 
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* '''Lane 13''':
 
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* '''Lane 14''':
 
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==Conclusion==
 
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The results show that the digest works, there is a band at approximately 541bp corresponding to ''yneA'' and a band at approximately 8.4kbp corresponding to pGFPrrnb in lanes 2,3,4,6,7,8,9, 11 and 12.
 
{{Team:Newcastle/footer}}
{{Team:Newcastle/footer}}

Latest revision as of 02:08, 28 October 2010

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Levan glue

We started working on levan. For this we used agar plates containing 10% sucrose and Bacillus subtilis 168 was grown onto that medium. The sucrose cultures and controls were compared. The cultures grown on sucrose should be considerably thicker.

The overnight cultures of the different strains were also grown on solid agar plates with and without sucrose. For more information levan, please refer to: levan.

B. subtilis producing levan

Image 1: Image showing B. subtilis producing levan on agar plate containing 10% sucrose.


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