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Team:Newcastle/Meetings/26 March 2010
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* Chair: Jannetta, Minutes: Rachel, Computer: Harsh | * Chair: Jannetta, Minutes: Rachel, Computer: Harsh | ||
*Present: Jen, Janetta, Neil, Steven,Younus, Da, Alan, Richard, Harsh | *Present: Jen, Janetta, Neil, Steven,Younus, Da, Alan, Richard, Harsh | ||
| - | *Apologies: Phil ( | + | *Apologies: Phil (holiday), Jem and Matt |
*Approval of agenda and minutes - Approved | *Approval of agenda and minutes - Approved | ||
| + | *[[Team:Newcastle/Agendas/26_March_2010| Agenda]] | ||
====Research Feedback==== | ====Research Feedback==== | ||
| Line 12: | Line 13: | ||
#*High compressive strength low tensile strength | #*High compressive strength low tensile strength | ||
#*Steal added good for temp change | #*Steal added good for temp change | ||
| - | #*CaCO<sub>3</sub> – similar to concrete; expand same as concrete | + | #*CaCO<sub>3</sub> – similar to concrete; expand same as concrete - might crack like concrete |
| - | #*Spider silk like steal- reduce possibility of cracking | + | #*Spider silk like steal - reduce possibility of cracking |
| - | #*Glue- no many papers- expansion may not be a problem? | + | #*Glue - no many papers - expansion may not be a problem? |
#*Silk alkali resistant | #*Silk alkali resistant | ||
#*Capillary properties of concrete change over time- in project we consider small rapid | #*Capillary properties of concrete change over time- in project we consider small rapid | ||
| Line 22: | Line 23: | ||
#*Simple issue | #*Simple issue | ||
#*Not correct term | #*Not correct term | ||
| - | #*Swimming Bacillus | + | #*Swimming ''Bacillus'' |
#*Oxygen gradient big selling points | #*Oxygen gradient big selling points | ||
#'''Genetics Homologous recombination plasmid chromosome''' (Harsh and Rachel) | #'''Genetics Homologous recombination plasmid chromosome''' (Harsh and Rachel) | ||
#*Test in plasmids- Find good plasmid | #*Test in plasmids- Find good plasmid | ||
| - | #*Integrate into genome | + | #*Integrate into genome |
#*Recombination site... Find!!! | #*Recombination site... Find!!! | ||
#*amyE site amylase with insert can’t break down amylase | #*amyE site amylase with insert can’t break down amylase | ||
| - | |||
#'''Nutrients''' (Harsh and Rachel) | #'''Nutrients''' (Harsh and Rachel) | ||
#*Bacteria algae growth living in concrete | #*Bacteria algae growth living in concrete | ||
| Line 36: | Line 36: | ||
#*Biofilms exclude other not algae | #*Biofilms exclude other not algae | ||
#*Antifungals, antialgaes, antibiotic | #*Antifungals, antialgaes, antibiotic | ||
| - | |||
#'''Sporulation, germination and secretion control''' (Alan and Da) | #'''Sporulation, germination and secretion control''' (Alan and Da) | ||
#*SpoA – last years team sporulation control, maybe turn off completely | #*SpoA – last years team sporulation control, maybe turn off completely | ||
| Line 43: | Line 42: | ||
#*Inducible promoter | #*Inducible promoter | ||
#*Glue out of bug | #*Glue out of bug | ||
| - | + | #'''Quorum sensing''' (Younus and Phil) | |
| - | '''Quorum sensing''' (Younus and Phil) | + | #*CompX and cff |
| - | *CompX and cff | + | #*Two-component system comP |
| - | *Two-component system comP | + | #'''Kill switch''' (Richard and Jannetta) |
| - | + | #*Why ? Don’t want?- not die after job fill new cracks | |
| - | '''Kill switch''' (Richard and Jannetta) | + | #*Nuclease |
| - | *Why ? Don’t want?- not die after job fill new cracks | + | #*Phil mazEF |
| - | *Nuclease | + | #*Skf SbB |
| - | *Phil | + | #*If linear DNA works |
| - | *Skf SbB | + | #'''Glue, CaCo3 and spider silk''' (Zoltan and Jannetta) |
| - | *If linear DNA works | + | #*Bacteria that produce CaCo3 from urea |
| - | + | #*How secrete spider silk long complex proteins – how can we make it happen in bacillus | |
| - | + | #*Secretion systems | |
| - | '''Glue, CaCo3 and spider silk''' (Zoltan and Jannetta) | + | #'''Other points''' |
| - | *Bacteria that produce CaCo3 from urea | + | #*Linear DNA – Richard |
| - | *How secrete spider silk long complex proteins – how can we make it happen in bacillus | + | #*Justifications of Linear |
| - | *Secretion systems | + | #*Build it up the use |
| - | + | #*Mutations | |
| - | '''Other points''' | + | #*Not specifically a problem – keep thinking about it though |
| - | *Linear DNA – Richard | + | |
| - | Justifications of Linear | + | |
| - | Build it up the use | + | |
| - | Mutations | + | |
| - | Not specifically a problem – keep thinking about it though | + | |
====Action points==== | ====Action points==== | ||
| - | *All - Read | + | *All - Read Voigt paper |
*All - Next week working off the proper wiki | *All - Next week working off the proper wiki | ||
*Janetta contact details doc in drop box | *Janetta contact details doc in drop box | ||
*Janetta and Richard – Flow chart!!! | *Janetta and Richard – Flow chart!!! | ||
*Reminder to neil and jen not welcome trust funding by 2nd of April? | *Reminder to neil and jen not welcome trust funding by 2nd of April? | ||
| - | *Janetta- T-shirt sponsors and logo – embroidered / print mixed – 5 or 6 sponsors design your own- printing cheaper , quotes dependent on design | + | *Janetta - T-shirt sponsors and logo – embroidered / print mixed – 5 or 6 sponsors design your own- printing cheaper, quotes dependent on design |
| - | *Jannetta- send us links for graphic design software- please | + | *Jannetta - send us links for graphic design software- please |
*Transfer the wiki | *Transfer the wiki | ||
*Individual iGEM applications - Phil hasn’t applied; Richard not accepted; Janetta text Phil | *Individual iGEM applications - Phil hasn’t applied; Richard not accepted; Janetta text Phil | ||
*Jen/Neil - Pay for team!! | *Jen/Neil - Pay for team!! | ||
| - | *Visas/Passport update - Harsh will apply May/June; Richard needs to get a visa waiver | + | *Visas/Passport update - Harsh will apply May/June; Richard needs to get a visa waiver |
| + | |||
====Wiki transfer==== | ====Wiki transfer==== | ||
*Clarify contents of identified pages | *Clarify contents of identified pages | ||
| Line 87: | Line 82: | ||
*Identify other pages required | *Identify other pages required | ||
*Ideas for layout/theme | *Ideas for layout/theme | ||
| - | *Zoltan Tidy it up-Urgent | + | *Zoltan - Tidy it up - Urgent |
*Borrow last years / Look at anything thats nice | *Borrow last years / Look at anything thats nice | ||
| - | *It evolves!!! We can change it | + | *It evolves!!! We can change it |
====Travel plans==== | ====Travel plans==== | ||
*Flights and Accomodation | *Flights and Accomodation | ||
| Line 97: | Line 92: | ||
*Main chemical company – free stuff? | *Main chemical company – free stuff? | ||
====Advisors/additional team members - civil engineer/graphics designer==== | ====Advisors/additional team members - civil engineer/graphics designer==== | ||
| - | *Still looking – Neil,Jen | + | *Still looking – Neil, Jen |
| + | |||
====Tutorials==== | ====Tutorials==== | ||
#Sequentia and Clone manager - basics | #Sequentia and Clone manager - basics | ||
| Line 107: | Line 103: | ||
*Web services for Biosynthetics, Flow chart | *Web services for Biosynthetics, Flow chart | ||
*Taverner | *Taverner | ||
| - | ==== | + | ====Timeline discussion==== |
*Need someone else on time line. | *Need someone else on time line. | ||
*16th of June Lab induction | *16th of June Lab induction | ||
*Deadlines soon | *Deadlines soon | ||
| - | *Change flight deadline – not 31st | + | *Change flight deadline – not 31st |
| - | + | ||
====Other business==== | ====Other business==== | ||
*PDF mark up tools – PDF exchange viewer, foxit, keeps mark-up | *PDF mark up tools – PDF exchange viewer, foxit, keeps mark-up | ||
Latest revision as of 10:24, 27 October 2010
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Contents |
Formal meeting - 26th March 2010
- Room: CBCB; Start: 11 am
- Chair: Jannetta, Minutes: Rachel, Computer: Harsh
- Present: Jen, Janetta, Neil, Steven,Younus, Da, Alan, Richard, Harsh
- Apologies: Phil (holiday), Jem and Matt
- Approval of agenda and minutes - Approved
- Agenda
Research Feedback
- Properties of concrete and surface tension (Zoltan and Steven)
- High compressive strength low tensile strength
- Steal added good for temp change
- CaCO3 – similar to concrete; expand same as concrete - might crack like concrete
- Spider silk like steal - reduce possibility of cracking
- Glue - no many papers - expansion may not be a problem?
- Silk alkali resistant
- Capillary properties of concrete change over time- in project we consider small rapid
- Cement water ratio and surface area and environment
- Where to look for the cracks? Crack pattern
- Microinjection (Zoltan and Steven)
- Simple issue
- Not correct term
- Swimming Bacillus
- Oxygen gradient big selling points
- Genetics Homologous recombination plasmid chromosome (Harsh and Rachel)
- Test in plasmids- Find good plasmid
- Integrate into genome
- Recombination site... Find!!!
- amyE site amylase with insert can’t break down amylase
- Nutrients (Harsh and Rachel)
- Bacteria algae growth living in concrete
- Only activate feed bacillus
- Two hybrid
- Biofilms exclude other not algae
- Antifungals, antialgaes, antibiotic
- Sporulation, germination and secretion control (Alan and Da)
- SpoA – last years team sporulation control, maybe turn off completely
- Nutrient availability
- KinA
- Inducible promoter
- Glue out of bug
- Quorum sensing (Younus and Phil)
- CompX and cff
- Two-component system comP
- Kill switch (Richard and Jannetta)
- Why ? Don’t want?- not die after job fill new cracks
- Nuclease
- Phil mazEF
- Skf SbB
- If linear DNA works
- Glue, CaCo3 and spider silk (Zoltan and Jannetta)
- Bacteria that produce CaCo3 from urea
- How secrete spider silk long complex proteins – how can we make it happen in bacillus
- Secretion systems
- Other points
- Linear DNA – Richard
- Justifications of Linear
- Build it up the use
- Mutations
- Not specifically a problem – keep thinking about it though
Action points
- All - Read Voigt paper
- All - Next week working off the proper wiki
- Janetta contact details doc in drop box
- Janetta and Richard – Flow chart!!!
- Reminder to neil and jen not welcome trust funding by 2nd of April?
- Janetta - T-shirt sponsors and logo – embroidered / print mixed – 5 or 6 sponsors design your own- printing cheaper, quotes dependent on design
- Jannetta - send us links for graphic design software- please
- Transfer the wiki
- Individual iGEM applications - Phil hasn’t applied; Richard not accepted; Janetta text Phil
- Jen/Neil - Pay for team!!
- Visas/Passport update - Harsh will apply May/June; Richard needs to get a visa waiver
Wiki transfer
- Clarify contents of identified pages
- Ethics page
- Research page
- Safety page
- Identify other pages required
- Ideas for layout/theme
- Zoltan - Tidy it up - Urgent
- Borrow last years / Look at anything thats nice
- It evolves!!! We can change it
Travel plans
- Flights and Accomodation
- Need to sort out who is going
Funding
- Harsh has made draft letter
- Main chemical company – free stuff?
Advisors/additional team members - civil engineer/graphics designer
- Still looking – Neil, Jen
Tutorials
- Sequentia and Clone manager - basics
- Modelling part two
What next?
- Flow chart of what we’re going to do Big picture!!!
- If its coloured life is easier
- Cambridge brick - Colour
- Web services for Biosynthetics, Flow chart
- Taverner
Timeline discussion
- Need someone else on time line.
- 16th of June Lab induction
- Deadlines soon
- Change flight deadline – not 31st
Other business
- PDF mark up tools – PDF exchange viewer, foxit, keeps mark-up
- Making dropbox use better
Items for next agenda
- Need wiki transferred by next meeting
- Review of flow chart if done
Next meeting
- Thursday early as possible – email to organise
- Chair: Da, Minutes: Alan, Computer: Younus
