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Team:UPO-Sevilla/Notebook/10 01
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<h1>October, 1st</h1> | <h1>October, 1st</h1> | ||
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<p>Different samples were run in a 0.8% or 1.5% agarose gel. UPO13+3, 12+2 and 1+2+16+3 were confirmed.</p> | <p>Different samples were run in a 0.8% or 1.5% agarose gel. UPO13+3, 12+2 and 1+2+16+3 were confirmed.</p> | ||
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<p>We have checked different preparations of 1+2 and 12+2. Different samples of 1+2 were digested using SspI-SpeI and these digestions were run in a 1.5% agarose gel. PCRs of 12+2 (several versions) and 12 were made and were run in a 0.8% polyacrilamide/TBE gel. UPO1+2 was confirmed; but UPO12+2 results were negative. UPO12+2 primers were designed.</p> | <p>We have checked different preparations of 1+2 and 12+2. Different samples of 1+2 were digested using SspI-SpeI and these digestions were run in a 1.5% agarose gel. PCRs of 12+2 (several versions) and 12 were made and were run in a 0.8% polyacrilamide/TBE gel. UPO1+2 was confirmed; but UPO12+2 results were negative. UPO12+2 primers were designed.</p> | ||
| - | <p>Digestion of UPO 7, 7+3, 13+3 and 16+3 (2h, 37ºC) to clone them at pSB4K5. Digestions were run in an agarose gel and positive spots (all of them except 13+3) were isolated and purified using GFX. Ligation and transformation in | + | <p>Digestion of UPO 7, 7+3, 13+3 and 16+3 (2h, 37ºC) to clone them at pSB4K5. Digestions were run in an agarose gel and positive spots (all of them except 13+3) were isolated and purified using GFX. Ligation and transformation in DH5α.</p> |
Latest revision as of 15:12, 27 October 2010
October, 1st
Assembly Team
Different samples were run in a 0.8% or 1.5% agarose gel. UPO13+3, 12+2 and 1+2+16+3 were confirmed.
We have checked different preparations of 1+2 and 12+2. Different samples of 1+2 were digested using SspI-SpeI and these digestions were run in a 1.5% agarose gel. PCRs of 12+2 (several versions) and 12 were made and were run in a 0.8% polyacrilamide/TBE gel. UPO1+2 was confirmed; but UPO12+2 results were negative. UPO12+2 primers were designed.
Digestion of UPO 7, 7+3, 13+3 and 16+3 (2h, 37ºC) to clone them at pSB4K5. Digestions were run in an agarose gel and positive spots (all of them except 13+3) were isolated and purified using GFX. Ligation and transformation in DH5α.
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