Team:Caltech/Protocols

(Difference between revisions)

Latest revision as of 23:20, 24 October 2010

iGEM 2010

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Project Details

Protocols

Completed Systems

Notebook

Biosafety

Human Impact

References

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-* Making V1012 competent
+{{Caltech_iGEM_10|
-* Making stock solutions
+Content=
-* Freezer (glycerol) stocks
+__NOTOC__
-* Plating antibiotics
+* [[Team:Caltech/Competent Cells|Competent Cells]]
-* Sequencing primers
+* [[Team:Caltech/Electroporation|Transformation by Electroporation]]
-* Making synthase casette
+* [[Team:Caltech/Glycerol Stocks|Freezer (glycerol) stocks]]
-** Sources of sequences
+* [[Team:Caltech/PlatingAntibiotics|Plating antibiotics]]
-** Optimizing & checking genes
+* [[Team:Caltech/SeqPrimers|Sequencing primers]]
-* Chemical/Crosslinking reactions
+<!--* [[Team:Caltech/MakingPlastic|Growing cells with PHA (and substrate oil)]]-->
-** Digesting substrate (hydrolysis) + epoxidation (MCPBA)
+* [[Team:Caltech/Colony PCR|Colony PCR]]
-** crosslinking (AIBN3) + UV initiation
+* [[Team:Caltech/Cloning|Two-day Cloning]]
-* Inducing lysis via 632nm light initiation
+}}
-* Making cell/glycerol/agar mixture
-** Applying this to plates