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Revision as of 15:23, 25 October 2010 (view source) Neima (Talk | contribs) ← Older edit		Latest revision as of 15:42, 25 October 2010 (view source) Neima (Talk | contribs) m
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-	'''Concentrations for antibiotics '''	+	'''Antibiotics'''
-	'''Antibiotic'''''' Stock''' '''  E.coli '''    '''  B.subtilis'''	+	[[Ampicillin]]
-	*[[Ampicillin]] (Amp) (in H2O)     50 mg/ml (4 °C)     100(μg/ml) (:500)	+	100 mg/ml Ampicillin (1000x) Stock
		+	*1 g of Ampicillin sodium salt in 10 mL of demiwater (or 50% EtOH)
		+	*Add NaOH or KOH to allow the Ampicillin to dissolve
		+	*Filter sterilize 0.2 μm filter and aliquot
		+	*Store -20 °C
-	*[[Kanamycin]] (Km)             10 mg/ml (4 °C)     20 (μg/ml) (:500)                    10 (μg/ml) (:100)	+	[[Chloramphenicol]]
-	*[[Chloramphenicol]](Cm) (50% EtOH)    5 mg/ml (-18 °C)     10-15 (μg/ml) (:500/333)         5(μg/ml) (:1000)	+	35 mg/ml Chloramphenicol (1000x) Stock
		+	*0.35 g in 10 mL 100% EtOH
		+	*Filter sterilize 0.2 μm filter and aliquot
		+	*Store -20 °C
		+
		+	[[Kanamycin]]
		+
		+	50 mg/ml Kanamycin (1000x) Stock
		+	*500 mg in 10 mL demi water
		+	*Filter sterilize 0.2 μm filter and aliquot
		+	*Store -20 °C

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Latest revision as of 15:42, 25 October 2010

Antibiotics

Ampicillin

100 mg/ml Ampicillin (1000x) Stock

• 1 g of Ampicillin sodium salt in 10 mL of demiwater (or 50% EtOH)
• Add NaOH or KOH to allow the Ampicillin to dissolve
• Filter sterilize 0.2 μm filter and aliquot
• Store -20 °C

Chloramphenicol

35 mg/ml Chloramphenicol (1000x) Stock

• 0.35 g in 10 mL 100% EtOH
• Filter sterilize 0.2 μm filter and aliquot
• Store -20 °C

Kanamycin

50 mg/ml Kanamycin (1000x) Stock

• 500 mg in 10 mL demi water
• Filter sterilize 0.2 μm filter and aliquot
• Store -20 °C