Team:Newcastle/Meetings/1 July 2010

From 2010.igem.org

(Difference between revisions)
(Roll call)
 
(17 intermediate revisions not shown)
Line 1: Line 1:
{{Team:Newcastle/mainbanner}}
{{Team:Newcastle/mainbanner}}
-
==Roll call==
+
==Formal Meeting - 1st July 2010==
-
Everyone here except Phil and Younas who are in the lab. Arrived 10mins later.
+
-
==Action points from last week==
+
*[[Team:Newcastle/Agendas/1_July_2010#Formal_Meeting_-_1st_July_2010| Agenda]]
 +
====Roll call====
 +
Everyone here except Phil and Younus who are in the lab. Arrived 10 mins later.
 +
 
 +
====Action points from last week====
* Wiki changes have been made.
* Wiki changes have been made.
-
* Letter to Andrew Young is not - someone needs to be in charge of this.
+
* Letter to Andrew Young is not finished - someone needs to be in charge of this.
* Civil engineer funding is not done yet.
* Civil engineer funding is not done yet.
-
* Flights plan is to get there on Thursday about 4-5pm.
+
* Flights: plan is to get there on Thursday about 4-5 p.m.
-
==Research feedback - end of crack signalling, quorum sensing==
+
====Research feedback - end of crack signalling, quorum sensing====
-
Diagram on board.
+
* Oxygen limitation triggers subtilin production, ''ResD'' and ''E''.
-
Oxygen limitation triggers subtilin production, ''ResD'' and ''E''. Need subtilin immunity brick too ''spaIFEG''. Subtilin receptor was done in 2008 by Newcastle ''spaRK''.
+
* Need subtilin immunity brick too ''spaIFEG''.
-
Want it to be triggered by both oxygen limitation and by subtilin. Oxygen to trigger the production at the end of the crack, subtilin to propagate the signal.
+
* Subtilin receptor was done in 2008 by Newcastle ''spaRK''.
-
Need to feedback subtilin to the production. Then it latches to production. Need to activate production in response to subtilin. Need the promotor at the end of the 2008 brick.
+
* Want it to be triggered by both oxygen limitation and by subtilin. Oxygen to trigger the production at the end of the crack, subtilin to propagate the signal.
-
Need a repressor to switch it off when oxygen is present.
+
* We need to get the strain with these genes. ATCC6633.
-
We need to get the strain with these genes. ATCC6633.
+
-
==Lab feedback==
+
====Lab feedback====
-
Arginine and urease tests - Harsh and Alan. The experiment has been designed, waiting for the materials. In the lab at 4.
+
*Arginine and urease tests - Harsh and Alan. The experiment has been designed, waiting for the materials. In the lab at 4 pm.
-
Urea media, grown overnight, If the urea is used, there will be a colour change.
+
*Urea media, grown overnight, If the urea is used, there will be a colour change.
-
Arginine test. Bug in LB, supplement with arginine. If arginine is used, pH increase.
+
*Arginine test. Bug in LB, supplement with arginine. If arginine is used, pH increase.
-
Phil and Y, transforming ''E. coli'' dh5alpha with the vector that we will be using for lacI. Plasmid extraction tomorrow.
+
*Phil and Younus, transforming ''E. coli'' dh5alpha with the vector that we will be using for lacI. Plasmid extraction tomorrow.
-
==Logo and T-shirts==
+
====Logo and T-shirts====
* Finalised.
* Finalised.
-
* University logo must be correct - take it from the University website, not from anywhere else.
+
* University logo must be correct - take it from the University website, not from anywhere else. Fill in the form to get the high resolution image.
 +
* CBCB logo needs to be added. Ask Jeff or Joanna.
 +
 
 +
====Visas====
 +
* Younus still hasn't filled in the visa-waiver form.
 +
 
 +
====Funding====
 +
* Andrew Young letter needs to be finished and checked tomorrow.
 +
 
 +
====Timeline====
 +
* Could be made more detailed now.
 +
* Might need another brick, for motility or swarming. Definitely do not want biofilm formation.
 +
* Spider silk next? It's on our logo so we should do it.
 +
 
 +
====Action points====
 +
* Phil: talk to Dr Aldridge about glue. How easy would it be to design a glue BioBrick?
 +
* All: Andrew Young letter needs to be finished and checked tomorrow.
 +
====Next meeting====
 +
*Next Wednesday, 3 p.m.
 +
*Chair: Harsh, Minutes: Alan, Computer: Phil
{{Team:Newcastle/footer}}
{{Team:Newcastle/footer}}

Latest revision as of 01:38, 26 October 2010

iGEM Homepage Newcastle University BacillaFilla Homepage Image Map

Contents

Formal Meeting - 1st July 2010

Roll call

Everyone here except Phil and Younus who are in the lab. Arrived 10 mins later.

Action points from last week

  • Wiki changes have been made.
  • Letter to Andrew Young is not finished - someone needs to be in charge of this.
  • Civil engineer funding is not done yet.
  • Flights: plan is to get there on Thursday about 4-5 p.m.

Research feedback - end of crack signalling, quorum sensing

  • Oxygen limitation triggers subtilin production, ResD and E.
  • Need subtilin immunity brick too spaIFEG.
  • Subtilin receptor was done in 2008 by Newcastle spaRK.
  • Want it to be triggered by both oxygen limitation and by subtilin. Oxygen to trigger the production at the end of the crack, subtilin to propagate the signal.
  • We need to get the strain with these genes. ATCC6633.

Lab feedback

  • Arginine and urease tests - Harsh and Alan. The experiment has been designed, waiting for the materials. In the lab at 4 pm.
  • Urea media, grown overnight, If the urea is used, there will be a colour change.
  • Arginine test. Bug in LB, supplement with arginine. If arginine is used, pH increase.
  • Phil and Younus, transforming E. coli dh5alpha with the vector that we will be using for lacI. Plasmid extraction tomorrow.

Logo and T-shirts

  • Finalised.
  • University logo must be correct - take it from the University website, not from anywhere else. Fill in the form to get the high resolution image.
  • CBCB logo needs to be added. Ask Jeff or Joanna.

Visas

  • Younus still hasn't filled in the visa-waiver form.

Funding

  • Andrew Young letter needs to be finished and checked tomorrow.

Timeline

  • Could be made more detailed now.
  • Might need another brick, for motility or swarming. Definitely do not want biofilm formation.
  • Spider silk next? It's on our logo so we should do it.

Action points

  • Phil: talk to Dr Aldridge about glue. How easy would it be to design a glue BioBrick?
  • All: Andrew Young letter needs to be finished and checked tomorrow.

Next meeting

  • Next Wednesday, 3 p.m.
  • Chair: Harsh, Minutes: Alan, Computer: Phil
Newcastle University logo.png    Newcastle cbcb logo.pngNewcastle Biomedicine logo.gif    Team Newcastle CEG logo.gif
Newcastle iww logo.jpg  UNIPV Pavia Logo.gif  Newcastle BBSRC.gif    Newcastle Genevision logo.png Newcastle WelcomeTrust.jpg
FaceBook Icon