Latest revision as of 17:19, 25 October 2010

Research

Today we started off our research on filamentous cells which will act as reinforcing fibres in the crack.

Dajkovic A., Mukherjee A. and Lutkenhaus J. (2008). "Investigation of Regulation of FtsZ Assembly by SulA and Development of a model for FtsZ Polymerization". Journal of Bacteriology". 190 (7). 2513- 2526.
Kawai Y. and Ogasawara N. (2006). "Bacillus subtilis ErzA and FtsL synergistically regulate FtsZ ring dynamics during cell division". Microbiology". 152. 1129- 1141.

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-=LacI BioBrick Construction=
+=Research=
-==Aims==
+Today we started off our research on filamentous cells which will act as reinforcing fibres in the crack.
-*To use PCR to extract ''lacI'' (promoter, ribosome-binding site (RBS) & coding sequence (CDS)) from plasmid pMutin4 and ligate into vector pSB1AT3 in front of red fluorescent protein (''rfp'').
-==Materials==
+#Dajkovic A., Mukherjee A. and Lutkenhaus J. (2008). "Investigation of Regulation of FtsZ Assembly by SulA and Development of a model for FtsZ Polymerization". ''Journal of Bacteriology". 190 (7). 2513- 2526.
-*Miniprep of pMutin4.
+#Kawai Y. and Ogasawara N. (2006). "''Bacillus subtilis'' ErzA and FtsL synergistically regulate FtsZ ring dynamics during cell division". ''Microbiology". 152. 1129- 1141.
-*Miniprep of pSB1AT3.
-==Protocol==
-* Set up a [[Team:Newcastle/Growing_an_overnight_cultures|overnight culture]] of colonies transformed with pMutin4 and pSB1AT3 to prepare stocks.
-==Inference==
-*Transformed ''E. coli'' DH5α grown up overnight to create a large stock of plasmid ready for extraction.
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