Team:Stockholm/13 October 2010
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I hope this will save some time since I am skipping the transformation into Top 10 cells. We don't have much time left of the competition therefore I hope this will work. | I hope this will save some time since I am skipping the transformation into Top 10 cells. We don't have much time left of the competition therefore I hope this will work. | ||
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+ | == Mimmi == | ||
+ | |||
+ | === Home page design === | ||
+ | |||
+ | *Bacteria figures for each link... | ||
+ | **saftey | ||
+ | **Sponsors | ||
+ | **Project | ||
+ | **Planning | ||
+ | **News | ||
+ | **Team | ||
+ | **Modelling | ||
+ | **Notebook | ||
+ | **Protocols | ||
+ | |||
+ | *to do (?): | ||
+ | **Results | ||
+ | **BioBricks | ||
+ | **Assemblies | ||
+ | |||
+ | |||
+ | |||
+ | === SOD - growth curve === | ||
+ | - ON culture | ||
+ | |||
+ | *SOD.his | ||
+ | *LMWP.SOD.his | ||
+ | *TAT.SOD.his | ||
+ | *Tra10.SOD.his | ||
+ | |||
+ | |||
+ | *3ml Amp<sub>100</sub> + toothpick of glycerol stock | ||
+ | |||
+ | {{Stockholm/Footer}} |
Latest revision as of 11:10, 26 October 2010
Contents |
Andreas
Plasmid prep
From 12/10 ON cultures
DNA concentration | ||
---|---|---|
Sample | Conc [ng/μl] | A260/A280 |
pSB1C3.IgGp | 237.9 | 1.89 |
pSB1C3.bFGF | 210.3 | 1.91 |
pSB1C3.ProtA | 173.6 | 1.89 |
pSB1C3.yCCS | 229.2 | 1.91 |
pSB1C3.SOD | 193.0 | 1.87 |
Sequencing
- 15 μl plasmid DNA; 1.5 μl primer (VR)
DNA concentration | ||
---|---|---|
Sample | Label | Sequence code |
pSB1C3.IgGp | pSB1C3.IgGp_VR | ASB0045 776 |
pSB1C3.bFGF | pSB1C3.bFGF_VR | ASB0045 777 |
pSB1C3.ProtA | pSB1C3.ProtA_VR | ASB0045 778 |
pSB1C3.yCCS | pSB1C3.yCCS_VR | ASB0045 779 |
pSB1C3.SOD | pSB1C3.SOD_VR | ASB0045 780 |
Nina
concentration measurement
I measured the concentration of the gel cleaned up samples from yesterday by spectrophotometry.
Ligation
I ligated the gel cleaned samples.
Transformation
I transformed the ligation samples into nine BL21 tubes each with 100 ul cells. I added 6 ul of the ligations to the cells and I thawed the tubes in the first step in 15 min.
I hope this will save some time since I am skipping the transformation into Top 10 cells. We don't have much time left of the competition therefore I hope this will work.
Mimmi
Home page design
- Bacteria figures for each link...
- saftey
- Sponsors
- Project
- Planning
- News
- Team
- Modelling
- Notebook
- Protocols
- to do (?):
- Results
- BioBricks
- Assemblies
SOD - growth curve
- ON culture
- SOD.his
- LMWP.SOD.his
- TAT.SOD.his
- Tra10.SOD.his
- 3ml Amp100 + toothpick of glycerol stock
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