Team:Harvard/flavor/parts

From 2010.igem.org

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<h2>Primers</h2>
<h2>Primers</h2>
<table cellspacing="0">
<table cellspacing="0">
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<tr><td><b>Name</b></td><td><b>Description</b></td></tr>
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<tr><td><b>Name</b></td><td><b>Description</b></td><td><b>Sequence</b></td></tr>
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<tr><td>J45004_F</td><td>Wintergreen pathway part. Encodes SAM benzoic acid/salicylic acid carboxyl methyltransferase I. Forward primer</td></tr>
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<tr><td>J45004_F</td><td>Wintergreen pathway part. Encodes SAM benzoic acid/salicylic acid carboxyl methyltransferase I. Forward primer</td><td><a href="https://2010.igem.org/Team:Harvard/J45004_F">link</a></td></tr>
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<tr><td>J45004_R</td><td>Wintergreen pathway part. Encodes SAM benzoic acid/salicylic acid carboxyl methyltransferase I. Reverse primer</td></tr>
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<tr><td>J45004_R</td><td>Wintergreen pathway part. Encodes SAM benzoic acid/salicylic acid carboxyl methyltransferase I. Reverse primer</td><td><a href="https://2010.igem.org/Team:Harvard/J45004_R">link</a></td></tr>
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<tr><td>J45017_F</td><td>Wintergreen pathway part. Encodes an isochorismate pyruvate-lyase and an isochorismate synthase. Forward primer</td></tr>
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<tr><td>J45017_F</td><td>Wintergreen pathway part. Encodes an isochorismate pyruvate-lyase and an isochorismate synthase. Forward primer</td><td><a href="https://2010.igem.org/Team:Harvard/J45017_F">link</a></td></tr>
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<tr><td>J45017_R</td><td>Wintergreen pathway part. Encodes an isochorismate pyruvate-lyase and an isochorismate synthase. Reverse primer</td></tr>
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<tr><td>J45017_R</td><td>Wintergreen pathway part. Encodes an isochorismate pyruvate-lyase and an isochorismate synthase. Reverse primer</td><td><a href="https://2010.igem.org/Team:Harvard/J45017_R">link</a></td></tr>
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<tr><td>NOSterm_BB_F</td><td>Plant specific terminator forward primer</td></tr>
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<tr><td>NOSterm_BB_F</td><td>Plant specific terminator forward primer</td><td><a href="https://2010.igem.org/Team:Harvard/NOSterm_BB_F">link</a></td></tr>
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<tr><td>NOSterm_BB_R</td><td>Plant specific terminator reverse primer</td></tr>
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<tr><td>NOSterm_BB_R</td><td>Plant specific terminator reverse primer</td><td><a href="https://2010.igem.org/Team:Harvard/NOSterm_BB_R">link</a></td></tr>
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<tr><td>Stop_NOSterm_BB_F</td><td>NOSt plant specific terminator with stop codon on end</td></tr>
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<tr><td>Stop_NOSterm_BB_F</td><td>NOSt plant specific terminator with stop codon on end</td><td><a href="https://2010.igem.org/Team:Harvard/Stop_NOSterm_BB_F">link</a></td></tr>
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<tr><td>pENTCUP2_BB_F</td><td>Plant specific promoter forward primer</td></tr>
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<tr><td>pENTCUP2_BB_F</td><td>Plant specific promoter forward primer</td><td><a href="https://2010.igem.org/Team:Harvard/pENTCUP2_BB_F">link</a></td></tr>
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<tr><td>pENTCUP2_BB_R</td><td>Plant specific promoter reverse primer</td></tr>
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<tr><td>pENTCUP2_BB_R</td><td>Plant specific promoter reverse primer</td><td><a href="https://2010.igem.org/Team:Harvard/pENTCUP2_BB_R">link</a></td></tr>
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<h2>Parts</h2>
<h2>Parts</h2>
<table cellspacing="0">
<table cellspacing="0">
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<tr><td><b>Name</b></td><td><b>Description</b></td></tr>
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<tr><td><b>Name</b></td><td><b>Description</b></td><td><b>Length</b></td><td><b>Sequence</b></td></tr>
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<tr><td>pENTCUP2 promoter</td><td>Plant specific promoter. Used to drive expression of our flavor constructs in plants. </td></tr>
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<tr><td>BBa_K382020</td><td><i>Arabidopsis</i> optimized Brazzein</td><td>195</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382020">link</a></td></tr>
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<tr><td>NOSt terminator</td><td>Plant specific terminator. Terminates transcription but no stop codon on end.</td></tr>
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<tr><td>BBa_K382021</td><td><i>Arabidopsis</i> optimized Miraculin</td><td>660</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382021">link</a></td></tr>
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<tr><td>NOSt terminator + stop</td><td>Plant specific terminator. Terminates transcription with stop codon on end.</td></tr>
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<tr><td>BBa_K382022</td><td>pENTCUP2 plant specific promoter</td><td>503</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382022">link</a></td></tr>
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<tr><td>Brazzein</td><td>Sweet-tasting protein. 54 amino acid sequence. </td></tr>
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<tr><td>BBa_K382023</td><td>NosT plant specific terminator</td><td>253</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382023">link</a></td></tr>
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<tr><td>Miraculin</td><td>Taste-inverting protein turns sour to sweet. 191 amino acid sequence</td></tr>
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<tr><td>BBa_K382024</td><td>NosT plant specific terminator with STOP codon</td><td>256</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382024">link</a></td></tr>
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<tr><td>Brazzein YFP2x C-terminus</td><td>Brazzein with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expression</td></tr>
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<tr><td>BBa_K382025</td><td><i>Arabidopsis</i> optimized Miraculin with StrepII N-terminus tag</td><td>696</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382025">link</a></td></tr>
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<tr><td>Miraculin YFP2x C-terminus</td><td>Miraculin with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expression</td></tr>
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<tr><td>BBa_K382026</td><td><i>Arabidopsis</i> optimized Brazzein with StrepII N-terminus tag</td><td>201</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382026">link</a></td></tr>
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<tr><td>Brazzein StrepII C-terminus</td><td>Brazzein with StrepII tag on C-terminus for Western Blot.</td></tr>
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<tr><td>BBa_K382027</td><td><i>Arabidopsis</i> optimized Miraculin with StrepII C-terminus tag</td><td>696</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382027">link</a></td></tr>
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<tr><td>Miraculin StrepII C-terminus</td><td>Miraculin with StrepII tag on C-terminus for Western Blot.</td></tr>
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<tr><td>BBa_K382028</td><td><i>Arabidopsis</i> optimized Brazzein with StrepII C-terminus tag</td><td>201</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382028">link</a></td></tr>

Latest revision as of 00:15, 27 October 2010


parts and primers

Primers

NameDescriptionSequence
J45004_FWintergreen pathway part. Encodes SAM benzoic acid/salicylic acid carboxyl methyltransferase I. Forward primerlink
J45004_RWintergreen pathway part. Encodes SAM benzoic acid/salicylic acid carboxyl methyltransferase I. Reverse primerlink
J45017_FWintergreen pathway part. Encodes an isochorismate pyruvate-lyase and an isochorismate synthase. Forward primerlink
J45017_RWintergreen pathway part. Encodes an isochorismate pyruvate-lyase and an isochorismate synthase. Reverse primerlink
NOSterm_BB_FPlant specific terminator forward primerlink
NOSterm_BB_RPlant specific terminator reverse primerlink
Stop_NOSterm_BB_FNOSt plant specific terminator with stop codon on endlink
pENTCUP2_BB_FPlant specific promoter forward primerlink
pENTCUP2_BB_RPlant specific promoter reverse primerlink

Parts

NameDescriptionLengthSequence
BBa_K382020Arabidopsis optimized Brazzein195link
BBa_K382021Arabidopsis optimized Miraculin660link
BBa_K382022pENTCUP2 plant specific promoter503link
BBa_K382023NosT plant specific terminator253link
BBa_K382024NosT plant specific terminator with STOP codon256link
BBa_K382025Arabidopsis optimized Miraculin with StrepII N-terminus tag696link
BBa_K382026Arabidopsis optimized Brazzein with StrepII N-terminus tag201link
BBa_K382027Arabidopsis optimized Miraculin with StrepII C-terminus tag696link
BBa_K382028Arabidopsis optimized Brazzein with StrepII C-terminus tag201link

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