Team:UNIPV-Pavia/Freezer

From 2010.igem.org

(Difference between revisions)
m
 
(277 intermediate revisions not shown)
Line 1: Line 1:
-
<table width="100%" border="0" >
+
<table width="100%" border="0">
<tr>
<tr>
     <td colspan="2">{{UNIPV-Pavia/header}}</td>
     <td colspan="2">{{UNIPV-Pavia/header}}</td>
</tr>
</tr>
-
<tr><td align="left" valign="top" width="15%">{{UNIPV-Pavia/menu}}</td><td valign=top>
+
<tr>
-
 
+
    <td colspan="2">{{UNIPV-Pavia/menuAlto}}</td>
 +
</tr>
 +
<tr><td valign=top>
<!-- Contenuti -->
<!-- Contenuti -->
{{UNIPV-Pavia/Style}}
{{UNIPV-Pavia/Style}}
<table border="0" align="center" width="100%"><tr><td align="justify" valign="top" style="padding:20px">
<table border="0" align="center" width="100%"><tr><td align="justify" valign="top" style="padding:20px">
<html><p align="center"><font size="4"><b>FREEZER MANAGEMENT</b></font></p></html><hr><br>
<html><p align="center"><font size="4"><b>FREEZER MANAGEMENT</b></font></p></html><hr><br>
 +
<div align="center">
 +
For a detailed correspondence between our BioBrick "wiki names" and submitted BioBrick codes take a look [https://2010.igem.org/Team:UNIPV-Pavia/Calendar/October/settimana3#October.2C_18th here].
 +
</div>
<table class="cont" border=0>
<table class="cont" border=0>
-
<tr><th>Biobrick or<br>ligation name</th><th>Description</th><th>Creation date</th><th>Strain</th><th>Vector</th><th>Quality control</th><th>Length</th><th>Store</th> </tr>
+
<tr>
-
 
+
<th>Biobrick name</th>
-
<!-- <tr><td><partinfo>Partname</partinfo></td><td>prova2</td><td>prova3</td><td>prova4</td><td>prova5</td><td>prova6</td> </tr> -->
+
<th>Description</th>
-
 
+
<th>Creation date</th>
-
 
+
<th>Strain</th>
 +
<th>Vector</th>
 +
<th>Quality control</th>
 +
<th>Length</th>
 +
<th>Store</th>
 +
</tr>
 +
<!-- Parti e BB richiesti per l'iGEM - BioBrick 2010-->
<tr>
<tr>
<td>
<td>
Line 27: Line 38:
<partinfo>pSB2K3</partinfo>
<partinfo>pSB2K3</partinfo>
</td><td>
</td><td>
-
Seq ok(MIT); orange pellet when contained in <partinfo>pSB2K3</partinfo>
+
Seq ok (MIT); orange pellet when contained in <partinfo>pSB2K3</partinfo>
</td>  
</td>  
<td>744 bp</td><td>BioBricks 2010</td>
<td>744 bp</td><td>BioBricks 2010</td>
Line 44: Line 55:
<partinfo>J63009</partinfo> (Amp)
<partinfo>J63009</partinfo> (Amp)
</td><td>
</td><td>
-
Seq partially confirmed(MIT) because it lacks a T at the 3', but the ADH1 terminator (i.e. <partinfo>BBa_K165010</partinfo>) is ok. The correct ApE file is here.
+
Seq partially confirmed (MIT) because it lacks a T at the 3', but the ADH1 terminator (i.e. <partinfo>BBa_K165010</partinfo>) is ok. The correct ApE file is here.
</td>  
</td>  
<td>253 bp</td><td>BioBricks 2010</td>
<td>253 bp</td><td>BioBricks 2010</td>
</tr>
</tr>
-
 
Line 63: Line 73:
<partinfo>pSB1AK3</partinfo>
<partinfo>pSB1AK3</partinfo>
</td><td>
</td><td>
-
Seq ok(MIT)
+
Seq ok (MIT).
</td>  
</td>  
<td>403 bp</td><td>BioBricks 2010</td>
<td>403 bp</td><td>BioBricks 2010</td>
Line 81: Line 91:
<partinfo>pSB1A1</partinfo>
<partinfo>pSB1A1</partinfo>
</td><td>
</td><td>
-
Seq ok(MIT 2009); length ok
+
Seq ok (MIT 2009); length ok.
</td>  
</td>  
<td>769 bp</td><td>BioBricks 2010</td>
<td>769 bp</td><td>BioBricks 2010</td>
Line 98: Line 108:
<partinfo>pSB1A2</partinfo>
<partinfo>pSB1A2</partinfo>
</td><td>
</td><td>
-
Seq ok(MIT)
+
Seq ok (MIT).
</td>  
</td>  
<td>406 bp</td><td>BioBricks 2010</td>
<td>406 bp</td><td>BioBricks 2010</td>
Line 116: Line 126:
<partinfo>pSB1A2</partinfo>
<partinfo>pSB1A2</partinfo>
</td><td>
</td><td>
-
Seq ok(MIT); length ok
+
Seq ok (MIT); length ok.
</td>  
</td>  
<td>664 bp</td><td>BioBricks 2010</td>
<td>664 bp</td><td>BioBricks 2010</td>
Line 133: Line 143:
<partinfo>pSB1A2</partinfo>
<partinfo>pSB1A2</partinfo>
</td><td>
</td><td>
-
Seq partially confirmed(MIT) for unknown reasons: the alignment is fully correct. The ApE file with the correct prefix and suffix is here. Length ok.
+
Seq partially confirmed (MIT) for unknown reasons: the alignment is fully correct. The ApE file with the correct prefix and suffix is here. Length ok.
</td>  
</td>  
<td>718 bp</td><td>BioBricks 2010</td>
<td>718 bp</td><td>BioBricks 2010</td>
</tr>
</tr>
 +
<tr>
 +
<td>
 +
<partinfo>BBa_K208001</partinfo>
 +
<br/>
 +
(stab-1)
 +
</td><td>
 +
phasin phaP<br/>(Silver Standard 5' and 3')</td>
 +
<td>
 +
24/06/2010
 +
</td><td>
 +
''E. coli'' ???
 +
</td><td>
 +
<partinfo>pSB3K3</partinfo>
 +
</td><td>
 +
Seq ok (BMR); length ok.
 +
</td>
 +
<td>579 bp</td><td>BioBricks 2010</td>
 +
</tr>
<tr>
<tr>
<td>
<td>
-
<strike style="color:red;">I0-1</strike>
+
<partinfo>BBa_K208001</partinfo>
 +
<br/>
 +
(stab-2)
</td><td>
</td><td>
-
<partinfo>BBa_K125500</partinfo> (E-S)<br>+<br><partinfo>BBa_B0015</partinfo> (E-X)
+
phasin phaP<br/>(Silver Standard 5' and 3')</td>
 +
<td>
 +
24/06/2010
</td><td>
</td><td>
-
11/06/2010
+
''E. coli'' ???
</td><td>
</td><td>
-
''E. coli'' DH5-alpha
+
<partinfo>pSB3K3</partinfo>
</td><td>
</td><td>
-
<partinfo>pSB1AK3</partinfo>
+
Seq ok (BMR); length ok.
 +
</td>
 +
<td>579 bp</td><td>BioBricks 2010</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td>
 +
<partinfo>BBa_J72007</partinfo>
 +
</td><td>
 +
BamHI methyltransferase encoding CRIM plasmid
 +
</td><td>
 +
01/07/2010
 +
</td><td>
 +
''E. coli'' ???
 +
</td><td>
 +
HC Cm resistance
</td><td>
</td><td>
-
Wrong clone
+
No plasmid in the clone.<br/>
 +
For more informations click <html><a href="https://2010.igem.org/Team:UNIPV-Pavia/Calendar/July/settimana3#July.2C_14th" target="_blank">here</a></html>.
</td>  
</td>  
-
<td></td><td>Ligations 2010</td>
+
<td></td><td>BioBricks 2010</td>
</tr>
</tr>
 +
<tr>
 +
<td>
 +
<partinfo>BBa_J72008</partinfo>
 +
</td><td>
 +
phi80 integration helper plasmid pInt80-649
 +
</td><td>
 +
01/07/2010
 +
</td><td>
 +
''E. coli'' ???
 +
</td><td>
 +
Plasmid (Amp 50 ug/ml resistance)
 +
</td><td>
 +
Length ok.
 +
</td>
 +
<td>6335 bp</td><td>BioBricks 2010</td>
 +
</tr>
<tr>
<tr>
<td>
<td>
-
I0-2
+
<partinfo>BBa_J72013</partinfo>
</td><td>
</td><td>
-
<partinfo>BBa_K125500</partinfo> (E-S)<br>+<br><partinfo>BBa_B0015</partinfo> (E-X)
+
BglII methyltransferase encoding CRIM plasmid
</td><td>
</td><td>
-
11/06/2010
+
01/07/2010
 +
</td><td>
 +
''E. coli'' ???
 +
</td><td>
 +
HC Cm resistance
 +
</td><td>
 +
No plasmid in the clone.<br/>
 +
For more informations click <html><a href="https://2010.igem.org/Team:UNIPV-Pavia/Calendar/July/settimana3#July.2C_15th" target="_blank">here</a></html>.
 +
</td>
 +
<td><!--2978 bp--></td>
 +
<td>BioBricks 2010</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td>
 +
<partinfo>BBa_K105012</partinfo>
 +
</td><td>
 +
10 aa flexible protein domain linker
 +
</td><td>
 +
06/08/2010
</td><td>
</td><td>
''E. coli'' DH5-alpha
''E. coli'' DH5-alpha
</td><td>
</td><td>
-
<partinfo>pSB1AK3</partinfo>
+
<partinfo>pSB1A2</partinfo>
</td><td>
</td><td>
-
Seq ok(BMR); length ok
+
-
</td>  
</td>  
-
<td>4036 bp</td><td>Ligations 2010</td>
+
<td>30 bp</td>
 +
<td>BioBricks 2010</td>
</tr>
</tr>
<tr>
<tr>
<td>
<td>
-
I1-1<br/>(not used)
+
<partinfo>BBa_J13002</partinfo>
</td><td>
</td><td>
-
<partinfo>BBa_E2050</partinfo> (E-S)<br>+<br><partinfo>BBa_K165018</partinfo> (E-X)
+
p(tetR)+RBS34
 +
</td>
 +
<td>
 +
16/08/2010
 +
</td>
 +
<td>
 +
''E. coli'' TOP10
 +
</td>
 +
<td>
 +
<partinfo>pSB1A2</partinfo>
 +
</td>
 +
<td>
 +
-
 +
</td>
 +
<td>74 bp</td>
 +
<td>BioBricks 2010</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td>
 +
<partinfo>BBa_I13522</partinfo>
 +
</td><td>
 +
Untagged GFP behind a constitutive promoter
 +
</td>
 +
<td>
 +
16/08/2010
 +
</td>
 +
<td>
 +
''E. coli'' TOP10
 +
</td>
 +
<td>
 +
<partinfo>pSB1A2</partinfo>
 +
</td>
 +
<td>
 +
-
 +
</td>
 +
<td>937 bp</td>
 +
<td>BioBricks 2010</td>
 +
</tr>
 +
 
 +
 
 +
<tr>
 +
<td>
 +
<font class="BBok">pSB1C3_RFP</font></td>
 +
<td>
 +
<partinfo>BBa_J04450</partinfo> <br/> in <partinfo>pSB1C3</partinfo>.
 +
</td>
 +
<td>
 +
??
 +
</td><td>
 +
''E. coli'' DH5alpha
 +
</td><td>
 +
<partinfo>pSB1C3</partinfo>
 +
</td>
 +
<td>
 +
 
 +
</td>
 +
<td>
 +
1069 bp
 +
</td>
 +
<td>2009 Distribution</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td>
 +
<font class="BBok">pSB1C3_ccdB</font></td>
 +
<td>
 +
<partinfo>BBa_P1010</partinfo> <br/> in <partinfo>pSB1C3</partinfo>.
 +
</td>
 +
<td>
 +
??
 +
</td><td>
 +
''E. coli'' DH5alpha
 +
</td><td>
 +
<partinfo>pSB1C3</partinfo>
 +
</td>
 +
<td>
 +
 
 +
</td>
 +
<td>
 +
675 bp
 +
</td>
 +
<td>2009 Distribution</td>
 +
</tr>
 +
 
 +
 
 +
<tr><td colspan="8"> </td></tr>
 +
 
 +
<!--Ligazioni fatte durante l'iGEM2010 -->
 +
<tr>
 +
<th>Name</th>
 +
<th>Ligation</th>
 +
<th>Creation date</th>
 +
<th>Strain</th>
 +
<th>Vector</th>
 +
<th>Quality control</th>
 +
<th>Length</th>
 +
<th>Description</th>
 +
</tr>
 +
 
 +
 
 +
<tr>
 +
<td>
 +
<font class="BBok">I0</font>
 +
<br/>(it was I0-2)
 +
<br/><partinfo>BBa_k300005</partinfo>
 +
</td><td>
 +
<partinfo>BBa_K125500</partinfo> (E-S)<br>+<br><partinfo>BBa_B0015</partinfo> (E-X)
</td><td>
</td><td>
11/06/2010
11/06/2010
Line 184: Line 375:
''E. coli'' DH5-alpha
''E. coli'' DH5-alpha
</td><td>
</td><td>
-
<partinfo>J63009</partinfo> (Amp)
+
<partinfo>pSB1AK3</partinfo>
</td><td>
</td><td>
-
not sequenced, length ok
+
Seq ok (BMR); length ok.
</td>  
</td>  
-
<td></td><td>Ligations 2010</td>
+
<td>854 bp</td>
 +
<td>GFPs + TT</td>
</tr>
</tr>
Line 194: Line 386:
<tr>
<tr>
<td>
<td>
-
I1-2
+
<font class="BBok">I1</font>
 +
<br/>
 +
(it was I1-2)
 +
<br/><partinfo>BBa_k300006</partinfo>
</td><td>
</td><td>
<partinfo>BBa_E2050</partinfo> (E-S)<br>+<br><partinfo>BBa_K165018</partinfo> (E-X)</td><td>
<partinfo>BBa_E2050</partinfo> (E-S)<br>+<br><partinfo>BBa_K165018</partinfo> (E-X)</td><td>
Line 203: Line 398:
<partinfo>J63009</partinfo> (Amp)
<partinfo>J63009</partinfo> (Amp)
</td><td>
</td><td>
-
Seq ok(BMR), with an additional barcode (25bp) after the stop codon of mOrange; length ok
+
Seq ok (BMR), with an additional barcode (25bp) after the stop codon of mOrange; length ok.
</td>  
</td>  
-
<td></td><td>Ligations 2010</td>
+
<td>1029 bp</td>
 +
<td>mOrange + NSL-ADH1 terminator</td>
</tr>
</tr>
Line 211: Line 407:
<tr>
<tr>
<td>
<td>
-
I2-1
+
<font class="BBok">I2</font>
 +
<br/>
 +
(it was I2-1)
</td><td>
</td><td>
<partinfo>BBa_P1004</partinfo> (E-S)<br>+<br><partinfo>BBa_B0015</partinfo> (E-X)
<partinfo>BBa_P1004</partinfo> (E-S)<br>+<br><partinfo>BBa_B0015</partinfo> (E-X)
Line 222: Line 420:
<partinfo>pSB1AK3</partinfo>
<partinfo>pSB1AK3</partinfo>
</td><td>
</td><td>
-
Seq ok(BMR); Cm resistance ok; length ok.
+
Seq ok (BMR); Cm resistance ok; length ok.
</td>  
</td>  
-
<td></td><td>Ligations 2010</td>
+
<td>906 bp</td>
 +
<td>Cm resistance + TT</td>
</tr>
</tr>
 +
<tr>
<tr>
<td>
<td>
-
I2-2<br/>(not used)
+
<font class="BBok">I3</font>
 +
<br/>
 +
(it was I3-1)
 +
<br/><partinfo>BBa_k300009</partinfo>
</td><td>
</td><td>
-
<partinfo>BBa_P1004</partinfo> (E-S)<br>+<br><partinfo>BBa_B0015</partinfo> (E-X)</td>
+
<partinfo>BBa_K081008</partinfo> (E-S)<br>+<br><partinfo>BBa_B0015</partinfo> (E-X)</td>
<td>
<td>
11/06/2010
11/06/2010
Line 240: Line 443:
<partinfo>pSB1AK3</partinfo>
<partinfo>pSB1AK3</partinfo>
</td><td>
</td><td>
-
not sequenced; Cm resistance ok
+
Seq TWO PLASMIDS IN THIS CLONE!(BMR), two overlapped traces; length ok.
</td>  
</td>  
-
<td></td><td>Ligations 2010</td>
+
<td>801 bp</td>
 +
<td>RBS + luxI + TT</td>
</tr>
</tr>
Line 248: Line 452:
<tr>
<tr>
<td>
<td>
-
I3-1
+
<font class="BBok">I4</font>
 +
<br/>
 +
(it was I4-2)
</td><td>
</td><td>
-
<partinfo>BBa_K081008</partinfo> (E-S)<br>+<br><partinfo>BBa_B0015</partinfo> (E-X)</td>
+
<partinfo>BBa_K165037</partinfo> (S-P)<br/>+<br/>I1 (X-P)</td>
<td>
<td>
-
11/06/2010
+
18/06/2010
</td><td>
</td><td>
''E. coli'' DH5-alpha
''E. coli'' DH5-alpha
Line 258: Line 464:
<partinfo>pSB1AK3</partinfo>
<partinfo>pSB1AK3</partinfo>
</td><td>
</td><td>
-
Seq TWO PLASMIDS IN THIS CLONE!(BMR), two overlapped traces; length ok
+
Seq ok (BMR); length ok.
</td>  
</td>  
-
<td></td><td>Ligations 2010</td>
+
<td>1439 bp</td>
 +
<td>pTEF + mOrange + NLS + terminator</td>
</tr>
</tr>
<tr>
<tr>
<td>
<td>
-
<strike style="color:red;">I3-2</strike>
+
<font class="BBok">I5</font>
 +
<br/>
 +
(it was I5-1)
 +
<br/><partinfo>BBa_k300008</partinfo>
</td><td>
</td><td>
-
<partinfo>BBa_K081008</partinfo> (E-S)<br>+<br><partinfo>BBa_B0015</partinfo> (E-X)</td>
+
I2 (E-S)<br>+<br><partinfo>BBa_J61001</partinfo> (E-X)</td>
<td>
<td>
-
11/06/2010
+
18/06/2010
</td><td>
</td><td>
-
''E. coli'' DH5-alpha
+
''E. coli'' TOP10
</td><td>
</td><td>
-
<partinfo>pSB1AK3</partinfo>
+
<partinfo>pSB1A2</partinfo>
</td><td>
</td><td>
-
Wrong clone
+
Seq ok (BMR); Cm resistance ok; length ok.
</td>  
</td>  
-
<td></td><td>Ligations 2010</td>
+
<td>1320 bp</td>
 +
<td>Cm resistance + TT + R6K ori</td>
</tr>
</tr>
<tr>
<tr>
<td>
<td>
-
I4-1<br/>(not used)
+
<font class="BBok">I6</font>
 +
<br/>
 +
(it was I6-2)
</td><td>
</td><td>
-
<partinfo>BBa_K165037</partinfo> (S-P)<br/>+<br/>I1 (X-P)</td>
+
I3 (E-S)<br>+<br><partinfo>BBa_T9002</partinfo> (E-X)</td>
<td>
<td>
18/06/2010
18/06/2010
Line 290: Line 503:
''E. coli'' DH5-alpha
''E. coli'' DH5-alpha
</td><td>
</td><td>
-
<partinfo>pSB1AK3</partinfo>
+
<partinfo>pSB1A3</partinfo>
</td><td>
</td><td>
-
not sequenced; length ok
+
Seq LONG PART(BMR), but the ligation worked and the sequenced portions are ok; length ok.
</td>  
</td>  
-
<td></td><td>Ligations 2010</td>
+
<td>2754 bp</td>
 +
<td>RBS + luxI + TT + HSL sensor</td>
</tr>
</tr>
 +
<tr>
<tr>
<td>
<td>
-
I4-2
+
<font class="BBok">I7</font>
 +
<br/>
 +
(it was I7-3)
 +
<br/><partinfo>BBa_k300024</partinfo>
</td><td>
</td><td>
-
<partinfo>BBa_K165037</partinfo> (S-P)<br/>+<br/>I1 (X-P)</td>
+
<partinfo>BBa_J23118</partinfo> (S-P)<br/>+<br/>I6 (X-P)</td>
<td>
<td>
-
18/06/2010
+
25/06/2010
 +
</td><td>
 +
''E. coli'' TOP10
 +
</td><td>
 +
<partinfo>BBa_J61002</partinfo><br/>
 +
(Amp)
 +
</td><td>
 +
Length ok.
 +
</td>
 +
<td>2795 bp</td>
 +
<td>constitutive promoter + RBS + luxI + TT + HSL sensor</td>
 +
</tr>
 +
 
 +
 
 +
<tr>
 +
<td>
 +
<font class="BBok">I8</font>
 +
<br/>
 +
(it was I8-5)
 +
<br/><partinfo>BBa_K300021</partinfo>
 +
</td><td>
 +
<partinfo>BBa_J23110</partinfo> (S-P)<br/>+<br/>I6 (X-P)</td>
 +
<td>
 +
25/06/2010
 +
</td><td>
 +
''E. coli'' TOP10
 +
</td><td>
 +
<partinfo>BBa_J61002</partinfo><br/>
 +
(Amp)
 +
</td><td>
 +
Length ok.
 +
</td>
 +
<td>2795 bp</td>
 +
<td>constitutive promoter + RBS + luxI + TT + HSL sensor</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td>
 +
<font class="BBok">I9</font>
 +
<br/>
 +
(it was I9-1)
 +
<br/><partinfo>BBa_K300022</partinfo>
 +
</td><td>
 +
<partinfo>BBa_J23114</partinfo> (S-P)<br/>+<br/>I6 (X-P)</td>
 +
<td>
 +
25/06/2010
 +
</td><td>
 +
''E. coli'' TOP10
 +
</td><td>
 +
<partinfo>BBa_J61002</partinfo><br/>
 +
(Amp)
 +
</td><td>
 +
Length ok.
 +
</td>
 +
<td>2795 bp</td>
 +
<td>constitutive promoter + RBS + luxI + TT + HSL sensor</td>
 +
</tr>
 +
 
 +
 
 +
<tr>
 +
<td>
 +
<font class="BBok">I10</font>
 +
<br/>
 +
(it was I10-1)
 +
<br/><partinfo>BBa_K300023</partinfo>
 +
</td><td>
 +
<partinfo>BBa_J23116</partinfo> (S-P)<br/>+<br/>I6 (X-P)</td>
 +
<td>
 +
25/06/2010
 +
</td><td>
 +
''E. coli'' TOP10
 +
</td><td>
 +
<partinfo>BBa_J61002</partinfo><br/>
 +
(Amp)
 +
</td><td>
 +
Length ok.
 +
</td>
 +
<td>2795 bp</td>
 +
<td>constitutive promoter + RBS + luxI + TT + HSL sensor</td>
 +
</tr>
 +
 
 +
 
 +
<tr>
 +
<td>
 +
<font class="BBok">I12</font>
 +
<br/>
 +
(it was I12-2)
 +
<br/><partinfo>BBa_k300019</partinfo>
 +
</td>
 +
<td>
 +
<partinfo>BBa_J23105</partinfo> (S-P)<br/>+<br/>I6 (X-P)
 +
</td>
 +
<td>
 +
16/07/2010
 +
</td><td>
 +
''E. coli'' TOP10
 +
</td><td>
 +
<partinfo>BBa_J61002</partinfo><br/>
 +
(Amp)
 +
</td><td>
 +
Seq ok (BMR), length ok.
 +
</td>
 +
<td>2795 bp</td>
 +
<td>constitutive promoter + RBS + luxI + TT + HSL sensor</td>
 +
</tr>
 +
 
 +
 
 +
<tr>
 +
<td>
 +
<font class="BBok">I14</font>
 +
<br/>
 +
(it was I14-1)
 +
<br/><partinfo>BBa_k300030</partinfo>
 +
</td>
 +
<td>
 +
<partinfo>BBa_J23118</partinfo> (S-P)<br/>+<br/>I3 (X-P)
 +
</td>
 +
<td>
 +
16/07/2010
</td><td>
</td><td>
''E. coli'' DH5-alpha
''E. coli'' DH5-alpha
</td><td>
</td><td>
 +
<partinfo>BBa_J61002</partinfo><br/>
 +
(Amp)
 +
</td><td>
 +
Seq ok (BMR), length ok.
 +
</td>
 +
<td>842 bp</td>
 +
<td>constitutive promoter + RBS + luxI + TT</td>
 +
</tr>
 +
 +
 +
 +
<tr>
 +
<td>
 +
<font class="BBok">RING</font>
 +
<br/>
 +
</td>
 +
<td>
 +
I5 (X-S) on itself:<br/>
 +
Cm resistance (<partinfo>BBa_P1004</partinfo>) + TT (<partinfo>BBa_B0015</partinfo>) + R6K ori (<partinfo>BBa_J61001</partinfo>)
 +
</td>
 +
<td>
 +
16/07/2010
 +
</td><td>
 +
''E. coli'' DH5-alpha
 +
</td><td>
 +
Plasmid (Cm resistance, conditional thermosensitive ORI; works in PIR strains)
 +
</td><td>
 +
Tested <html><a href="https://2010.igem.org/Team:UNIPV-Pavia/Calendar/July/settimana4#July.2C_19th" target="_blank">here</a></html>.
 +
</td>
 +
<td>
 +
1328 bp
 +
</td>
 +
<td>Cm resistance + TT + R6K ori</td>
 +
</tr>
 +
 +
<tr>
 +
<td>
 +
<font class="BBok">I15</font>
 +
<br/>
 +
(it was I15-1)
 +
<br/><partinfo>BBa_k300028</partinfo>
 +
</td>
 +
<td>
 +
<partinfo>BBa_J23110</partinfo> (S-P)<br/>+<br/>I3 (X-P)
 +
</td>
 +
<td>
 +
23/07/2010
 +
</td><td>
 +
''E. coli'' DH5-alpha
 +
</td><td>
 +
<partinfo>BBa_J61002</partinfo><br/>
 +
(Amp)
 +
</td><td>
 +
Length ok.
 +
</td>
 +
<td>842 bp</td>
 +
<td>constitutive promoter + RBS + luxI + TT</td>
 +
</tr>
 +
 +
 +
<tr>
 +
<td>
 +
<font class="BBok">I16</font>
 +
<br/>
 +
(it was I16-1)
 +
<br/><partinfo>BBa_k300029</partinfo>
 +
</td>
 +
<td>
 +
<partinfo>BBa_J23116</partinfo> (S-P)<br/>+<br/>I3 (X-P)
 +
</td>
 +
<td>
 +
16/07/2010
 +
</td><td>
 +
''E. coli'' DH5-alpha
 +
</td><td>
 +
<partinfo>BBa_J61002</partinfo><br/>
 +
(Amp)
 +
</td><td>
 +
Seq ok (BMR), length ok.
 +
</td>
 +
<td>842 bp</td>
 +
<td>constitutive promoter + RBS + luxI + TT</td>
 +
</tr>
 +
 +
 +
<tr>
 +
<td>
 +
<font class="BBok">I17</font>
 +
<br/>
 +
(it was I17-1)
 +
<br/><partinfo>BBa_k300025</partinfo>
 +
</td>
 +
<td>
 +
<partinfo>BBa_J23101</partinfo> (S-P)<br/>+<br/>I3 (X-P)
 +
</td>
 +
<td>
 +
16/07/2010
 +
</td><td>
 +
''E. coli'' DH5-alpha
 +
</td><td>
 +
<partinfo>BBa_J61002</partinfo><br/> (Amp)
 +
</td><td>
 +
Seq ok (BMR), length ok.
 +
</td>
 +
<td>842 bp</td>
 +
<td>constitutive promoter + RBS + luxI + TT</td>
 +
</tr>
 +
 +
 +
<tr>
 +
<td>
 +
<font class="BBok">I18</font>
 +
<br/>
 +
(it was I18-1)
 +
<br/><partinfo>BBa_k300026</partinfo>
 +
</td>
 +
<td>
 +
<partinfo>BBa_J23105</partinfo> (S-P)<br/>+<br/>I3 (X-P)
 +
</td>
 +
<td>
 +
16/07/2010
 +
</td><td>
 +
''E. coli'' DH5-alpha
 +
</td><td>
 +
<partinfo>BBa_J61002</partinfo><br/>
 +
(Amp)
 +
</td><td>
 +
Seq ok (BMR), length ok.
 +
</td>
 +
<td>842 bp</td>
 +
<td>constitutive promoter + RBS + luxI + TT</td>
 +
</tr>
 +
 +
 +
<tr>
 +
<td>
 +
<font class="BBok">I19</font>
 +
<br/>
 +
(it was I19-1)
 +
<br/><partinfo>BBa_k300027</partinfo>
 +
</td>
 +
<td>
 +
<partinfo>BBa_J23106</partinfo> (S-P)<br/>+<br/>I3 (X-P)
 +
</td>
 +
<td>
 +
16/07/2010
 +
</td><td>
 +
''E. coli'' DH5-alpha
 +
</td><td>
 +
<partinfo>BBa_J61002</partinfo><br/>
 +
(Amp)
 +
</td><td>
 +
Seq ok (BMR), length ok.
 +
</td>
 +
<td>842 bp</td>
 +
<td>constitutive promoter + RBS + luxI + TT</td>
 +
</tr>
 +
 +
 +
<tr>
 +
<td>
 +
<font class="BBok">I20</font>
 +
<br/>
 +
(it was I20M-1)
 +
<br/><partinfo>BBa_k300002</partinfo>
 +
</td>
 +
<td>
 +
Pha10S-1 (X-S) (infos <html><a href="https://2010.igem.org/Team:UNIPV-Pavia/Calendar/July/settimana5#July.2C_29th" target="_blank">here</a></html>) <br/>+<br/> <partinfo>pSB1AK3</partinfo> (X-S)
 +
</td>
 +
<td>
 +
13/08/2010
 +
</td>
 +
<td>
 +
''E. coli'' DH5-alpha
 +
</td>
 +
<td>
<partinfo>pSB1AK3</partinfo>
<partinfo>pSB1AK3</partinfo>
 +
</td>
 +
<td>
 +
Seq ok (BMR), length ok.
 +
</td>
 +
<td>
 +
576 bp
 +
</td>
 +
<td>Pha10S</td>
 +
</tr>
 +
 +
 +
<tr>
 +
<td>
 +
<font class="BBok">I21</font>
 +
<br/>
 +
(it was I21-4)
 +
<br/><partinfo>BBa_k300003</partinfo>
 +
</td>
 +
<td>
 +
PhaSS-1 (X-S) (infos <html><a href="https://2010.igem.org/Team:UNIPV-Pavia/Calendar/August/settimana2#August.2C_10th" target="_blank">here</a></html>) <br/>+<br/> <partinfo>pSB1A3</partinfo> (X-S)
 +
</td>
 +
<td>
 +
10/08/2010
</td><td>
</td><td>
-
Seq ok(BMR); length ok
+
''E. coli'' DH5-alpha
 +
</td><td>
 +
<partinfo>pSB1A3</partinfo>
 +
</td><td>
 +
Seq ok (BMR), length ok.
</td>  
</td>  
-
<td></td><td>Ligations 2010</td>
+
<td>
 +
576 bp
 +
</td>
 +
<td>PhaSS</td>
</tr>
</tr>
 +
<tr>
<tr>
<td>
<td>
-
I5
+
<font class="BBok">I22</font>
 +
<br/>
 +
(it was I22-1)
 +
<br/><partinfo>BBa_k300031</partinfo>
 +
</td>
 +
<td>
 +
<partinfo>BBa_K081009</partinfo> (E-S) <br/>+<br/> <partinfo>BBa_B0015</partinfo> (E-X)
 +
</td>
 +
<td>
 +
10/08/2010
</td><td>
</td><td>
-
I2 (E-S)<br>+<br><partinfo>BBa_J61001</partinfo> (E-X)</td>
+
''E. coli'' DH5-alpha
 +
</td><td>
 +
<partinfo>pSB1AK3</partinfo>
 +
</td><td>
 +
Length ok.
 +
</td>  
<td>
<td>
-
18/06/2010
+
825 bp
 +
</td>
 +
<td>RBS + lasI + terminator</td>
 +
</tr>
 +
 
 +
 
 +
<tr>
 +
<td>
 +
<font class="BBok">I23</font>
 +
<br/>
 +
(it was I23-1)
 +
</td>
 +
<td>
 +
<partinfo>BBa_R0062</partinfo> (S-P) <br/>+<br/> I3 (X-P)
 +
</td>
 +
<td>
 +
10/08/2010
</td><td>
</td><td>
''E. coli'' DH5-alpha
''E. coli'' DH5-alpha
Line 326: Line 899:
<partinfo>pSB1A2</partinfo>
<partinfo>pSB1A2</partinfo>
</td><td>
</td><td>
-
Seq ok(BMR); Cm resistance ok; length ok
+
Length ok.
</td>  
</td>  
-
<td></td><td>Ligations 2010</td>
+
<td>
 +
725 bp
 +
</td>
 +
<td>plux + RBS + luxI + TT</td>
</tr>
</tr>
 +
<tr>
<tr>
<td>
<td>
-
I6-2
+
<font class="BBok">I24</font>
 +
<br/>
 +
(it was I24-1)
 +
</td>
 +
<td>
 +
<partinfo>BBa_R0062</partinfo> (S-P) <br/>+<br/> I22 (X-P)
 +
</td>
 +
<td>
 +
13/08/2010
</td><td>
</td><td>
-
I3 (E-S)<br>+<br><partinfo>BBa_T9002</partinfo> (E-X)</td>
+
''E. coli'' DH5-alpha
 +
</td>
<td>
<td>
-
18/06/2010
+
<partinfo>pSB1A2</partinfo>
 +
</td>
 +
<td>
 +
Length ok.
 +
</td>
 +
<td>
 +
886 bp
 +
</td>
 +
<td>plux + RBS + lasI + terminator</td>
 +
</tr>
 +
 
 +
 
 +
<tr>
 +
<td>
 +
<font class="BBok">I26</font>
 +
<br/>
 +
(it was I26-2)
 +
<br/><partinfo>BBa_k300081</partinfo>
 +
</td>
 +
<td>
 +
<partinfo>BBa_K105012</partinfo> (S-P) <br/>+<br/> I21 (X-P)
 +
</td>
 +
<td>
 +
13/08/2010
</td><td>
</td><td>
''E. coli'' DH5-alpha
''E. coli'' DH5-alpha
 +
</td>
 +
<td>
 +
<partinfo>pSB1A2</partinfo>
 +
</td>
 +
<td>
 +
Seq ok (BMR), length ok.
 +
</td>
 +
<td>
 +
612 bp
 +
</td>
 +
<td>linker + PhaSS</td>
 +
</tr>
 +
 +
 +
<tr>
 +
<td>
 +
<font class="BBok">I29</font>
 +
<br/>
 +
(it was I29-1)
 +
</td>
 +
<td>
 +
<partinfo>BBa_T9002</partinfo> (S-P) <br/>+<br/> I23 (X-P)
 +
</td>
 +
<td>
 +
31/08/2010
 +
</td>
 +
<td>
 +
''E. coli'' TOP10
 +
</td>
 +
<td>
 +
<partinfo>pSB1A3</partinfo>
 +
</td>
 +
<td>
 +
Length ok.
 +
</td>
 +
<td>
 +
2678 bp
 +
</td>
 +
<td>HLS sensor + plux + RBS + luxI + TT</td>
 +
</tr>
 +
 +
 +
<tr>
 +
<td>
 +
I30
 +
</td>
 +
<td>
 +
<partinfo>BBa_T9002</partinfo> (S-P) <br/>+<br/> I24 (X-P)
 +
</td>
 +
<td>
 +
 +
</td>
 +
<td>
 +
''E. coli'' TOP10
 +
</td>
 +
<td>
 +
<partinfo>pSB1A3</partinfo>
 +
</td>
 +
<td>
 +
 +
</td>
 +
<td>
 +
2839 bp
 +
</td>
 +
<td>HSL sensor + plux + RBS + lasI + terminator</td>
 +
</tr>
 +
 +
 +
<tr>
 +
<td>
 +
<font class="BBok">I31</font>
 +
<br/>
 +
(it was I31-1)
 +
<br/><partinfo>BBa_K300079</partinfo>
 +
</td>
 +
<td>
 +
I20 (E-S) <br/>+<br/> <partinfo>BBa_K105012</partinfo> (E-X)
 +
</td>
 +
<td>
 +
23/08/2010
 +
</td>
 +
<td>
 +
''E. coli'' DH5-alpha
 +
</td>
 +
<td>
 +
<partinfo>pSB1A2</partinfo>
 +
</td>
 +
<td>
 +
Seq ok (BMR), length ok.
 +
</td>
 +
<td>
 +
612 bp
 +
</td>
 +
<td>Pha10S + linker</td>
 +
</tr>
 +
 +
 +
<tr>
 +
<td>
 +
<font class="BBok">I32</font>
 +
<br/>
 +
(it was I32-2)
 +
</td>
 +
<td>
 +
I20 (E-S) <br/>+<br/> I21 (E-X)
 +
</td>
 +
<td>
 +
23/08/2010
</td><td>
</td><td>
 +
''E. coli'' DH5-alpha
 +
</td>
 +
<td>
<partinfo>pSB1A3</partinfo>
<partinfo>pSB1A3</partinfo>
 +
</td>
 +
<td>
 +
Seq ok (BMR), length ok.
 +
</td>
 +
<td>
 +
1158 bp
 +
</td>
 +
<td>Pha10S + PhaSS</td>
 +
</tr>
 +
 +
<tr>
 +
<td>
 +
<font class="BBok">I33</font>
 +
<br/>
 +
(it was I33-1)
 +
</td>
 +
<td>
 +
I20 (E-S) <br/>+<br/> I0 (E-X)
 +
</td>
 +
<td>
 +
23/08/2010
</td><td>
</td><td>
-
Seq LONG PART(BMR), but the ligation worked and the sequenced portions are ok; length ok
+
''E. coli'' DH5-alpha
 +
</td>
 +
<td>
 +
<partinfo>pSB1AK3</partinfo>
 +
</td>
 +
<td>
 +
Seq ok (BMR), length ok.
</td>  
</td>  
-
<td></td><td>Ligations 2010</td>
+
<td>
 +
1436 bp
 +
</td>
 +
<td>Pha10S + GFPs + TT</td>
</tr>
</tr>
 +
<tr>
<tr>
<td>
<td>
-
I6-3<br/>(not used)
+
<font class="BBok">I34</font>
 +
<br/>
 +
(it was I34-1)
 +
</td>
 +
<td>
 +
I21 (E-S) <br/>+<br/> I0 (E-X)
 +
</td>
 +
<td>
 +
23/08/2010
</td><td>
</td><td>
-
I3 (E-S)<br>+<br><partinfo>BBa_T9002</partinfo> (E-X)</td>
+
''E. coli'' DH5-alpha
 +
</td>
<td>
<td>
-
18/06/2010
+
<partinfo>pSB1AK3</partinfo>
 +
</td>
 +
<td>
 +
Seq ok (BMR), length ok.
 +
</td>
 +
<td>
 +
1436 bp
 +
</td>
 +
<td>PhaSS + GFPs + TT</td>
 +
</tr>
 +
 
 +
 
 +
<tr>
 +
<td>
 +
<font class="BBok">I35</font>
 +
<br/>
 +
(it was I35-1)
 +
<br/><partinfo>BBa_K300080</partinfo>
 +
</td>
 +
<td>
 +
I21 (E-S) <br/>+<br/> <partinfo>BBa_K105012</partinfo> (E-X)
 +
</td>
 +
<td>
 +
23/08/2010
</td><td>
</td><td>
''E. coli'' DH5-alpha
''E. coli'' DH5-alpha
 +
</td>
 +
<td>
 +
<partinfo>pSB1A2</partinfo>
 +
</td>
 +
<td>
 +
Seq ok (BMR), length ok.
 +
</td>
 +
<td>
 +
612 bp
 +
</td>
 +
<td>PhaSS + linker</td>
 +
</tr>
 +
 +
 +
<tr>
 +
<td>
 +
<font class="BBok">I36</font>
 +
<br/>
 +
(it was I36-7)
 +
</td>
 +
<td>
 +
I21 (E-S) <br/>+<br/> I21 (E-X)
 +
</td>
 +
<td>
 +
24/08/2010
</td><td>
</td><td>
 +
''E. coli'' DH5-alpha
 +
</td>
 +
<td>
<partinfo>pSB1A3</partinfo>
<partinfo>pSB1A3</partinfo>
 +
</td>
 +
<td>
 +
Length ok.
 +
</td>
 +
<td>
 +
1158 bp
 +
</td>
 +
<td>PhaSS + PhaSS</td>
 +
</tr>
 +
 +
 +
<tr>
 +
<td>
 +
<font class="BBok">I37</font>
 +
<br/>
 +
(it was I37-1)
 +
</td>
 +
<td>
 +
INTEIN (E-S) <br/>+<br/> I0 (E-X)
 +
</td>
 +
<td>
 +
23/08/2010
</td><td>
</td><td>
-
not sequenced
+
''E. coli'' DH5-alpha
 +
</td>
 +
<td>
 +
<partinfo>pSB1AK3</partinfo>
 +
</td>
 +
<td>
 +
Seq ok (BMR), length ok.
</td>  
</td>  
-
<td></td><td>Ligations 2010</td>
+
<td>
 +
1367 bp
 +
</td>
 +
<td>INTEIN + GFPs + TT</td>
</tr>
</tr>
 +
<tr>
<tr>
<td>
<td>
-
<partinfo>BBa_K208001</partinfo> (stab-1)<br/>
+
<font class="BBok">I38</font>
 +
<br/>
 +
(it was I38-3)
 +
</td>
 +
<td>
 +
<partinfo>BBa_F2620</partinfo> (E-S) <br/>+<br/> <partinfo>BBa_B0034</partinfo> (E-X)
 +
</td>
 +
<td>
 +
23/08/2010
</td><td>
</td><td>
-
phasin phaP</td>
+
''E. coli'' DH5-alpha
 +
</td>
<td>
<td>
-
24/06/2010
+
<partinfo>pSB1A2</partinfo>
 +
</td>
 +
<td>
 +
Seq ok (BMR), length ok.
 +
</td>
 +
<td>
 +
1081 bp
 +
</td>
 +
<td>HSL inducible system</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td>
 +
<font class="BBok">I39</font>
 +
<br/>
 +
(it was I39-5)
 +
</td>
 +
<td>
 +
<partinfo>BBa_J13002</partinfo> (S-P) <br/>+<br/> I20 (X-P)
 +
</td>
 +
<td>
 +
24/08/2010
</td><td>
</td><td>
-
''E. coli'' ???
+
''E. coli'' DH5-alpha
 +
</td>
 +
<td>
 +
<partinfo>pSB1A2</partinfo>
 +
</td>
 +
<td>
 +
Seq ok (BMR), length ok.
 +
</td>
 +
<td>
 +
656 bp
 +
</td>
 +
<td>pTet + Pha10S</td>
 +
</tr>
 +
 
 +
 
 +
<tr>
 +
<td>
 +
<font class="BBok">I40</font>
 +
<br/>
 +
(it was I40-1)
 +
</td>
 +
<td>
 +
I26 (E-S) <br/>+<br/> INTEIN (E-X)
 +
</td>
 +
<td>
 +
25/08/2010
</td><td>
</td><td>
-
<partinfo>pSB3K3</partinfo>
+
''E. coli'' DH5-alpha
 +
</td>
 +
<td>
 +
pMA (HC Amp resistance)
 +
</td>
 +
<td>
 +
Length ok.
 +
</td>
 +
<td>
 +
1125 bp
 +
</td>
 +
<td>linker + PhaSS + INTEIN</td>
 +
</tr>
 +
 
 +
 
 +
<tr>
 +
<td>
 +
<font class="BBok">I41</font>
 +
<br/>
 +
(it was I41-1)
 +
</td>
 +
<td>
 +
I26 (E-S) <br/>+<br/> I0 (E-X)
 +
</td>
 +
<td>
 +
23/08/2010
</td><td>
</td><td>
-
length ok
+
''E. coli'' DH5-alpha
 +
</td>
 +
<td>
 +
<partinfo>pSB1AK3</partinfo>
 +
</td>
 +
<td>
 +
Seq ok (BMR), length ok.
</td>  
</td>  
-
<td>579 bp</td><td>BioBricks 2010</td>
+
<td>
 +
1472 bp
 +
</td>
 +
<td>linker + PhaSS + GFPs + TT</td>
</tr>
</tr>
<tr>
<tr>
<td>
<td>
-
<partinfo>BBa_K208001</partinfo> (stab-2)<br/>
+
<font class="BBok">I42</font><br/>
 +
(it was I42-3)
 +
<br/>
 +
</td>
 +
<td>
 +
CREAM (E-P) <br/>+<br/> <partinfo>BBa_K173001</partinfo> (E-P)
 +
</td>
 +
<td>
 +
23/08/2010
</td><td>
</td><td>
-
phasin phaP</td>
+
''E. coli'' BW23474
 +
</td>
<td>
<td>
-
24/06/2010
+
CREAM
 +
</td>
 +
<td>
 +
Length ok.
 +
</td>
 +
<td>
 +
3068 bp
 +
</td>
 +
<td>CREAM fragment + Cm resistance + TT + R6K ori + constitutive GFP</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td>
 +
<font class="BBok">I43</font><br/><br/>
 +
(it was I43-2)
 +
<br/>
 +
</td>
 +
<td>
 +
CREAM (E-P) <br/>+<br/><partinfo>BBa_J23101</partinfo> (E-P)
 +
</td>
 +
<td>
 +
23/08/2010
</td><td>
</td><td>
-
''E. coli'' ???
+
''E. coli'' BW23474
 +
</td>
 +
<td>
 +
CREAM
 +
</td>
 +
<td>
 +
Length ok.
 +
</td>
 +
<td>
 +
3026 bp
 +
</td>
 +
<td>CREAM fragment + Cm resistance + TT + R6K ori + constitutive promoter</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td>
 +
<font class="BBok">I44</font>
 +
<br/>
 +
(it was I44-2)
 +
<br/>
 +
</td>
 +
<td>
 +
CREAM (E-P) <br/>+<br/><partinfo>BBa_I52002</partinfo> (E-P)
 +
</td>
 +
<td>
 +
15/09/2010
</td><td>
</td><td>
-
<partinfo>pSB3K3</partinfo>
+
''E. coli'' DB3.1
 +
</td>
 +
<td>
 +
CREAM
 +
</td>
 +
<td>
 +
Seq ok (BMR).
 +
</td>
 +
<td>
 +
3239 bp
 +
</td>
 +
<td>CREAM fragment + Cm resistance + TT + R6K ori + marker ccdB</td>
 +
</tr>
 +
 
 +
 
 +
<tr>
 +
<td>
 +
I45<br/>
 +
FAILED
 +
<br/>
 +
</td>
 +
<td>
 +
CREAM (HindIII) on itself
 +
</td>
 +
<td>
 +
23/08/2010
</td><td>
</td><td>
-
length ok
+
''E. coli'' BW23474
 +
</td>
 +
<td>
 +
CREAM
 +
</td>
 +
<td>
 +
 
</td>  
</td>  
-
<td>579 bp</td><td>BioBricks 2010</td>
+
<td>
 +
2149 bp
 +
</td>
 +
<td>CREAM fragment + Cm resistance + TT + R6K ori</td>
</tr>
</tr>
 +
<tr>
<tr>
<td>
<td>
-
I7-1<br/>
+
<font class="BBok">I47</font>
 +
<br/>
 +
(it was I47-13)
 +
<br/><partinfo>BBa_K300086</partinfo>
 +
<br/>
 +
</td>
 +
<td>
 +
<partinfo>BBa_J13002</partinfo> (S-P) + I33 (X-P)
 +
</td>
 +
<td>
 +
02/09/2010
</td><td>
</td><td>
-
<partinfo>BBa_J23118</partinfo> (E-S)<br/>+<br/>I6-2 (X-P)</td>
+
''E. coli'' TOP10
 +
</td>
<td>
<td>
-
25/06/2010
+
<partinfo>pSB1A2</partinfo>
 +
</td>
 +
<td>
 +
Seq ok (BMR), length ok.
 +
</td>
 +
<td>
 +
1516 bp
 +
</td>
 +
<td>pTet + Pha10S + GFPs + TT</td>
 +
</tr>
 +
 
 +
 
 +
<tr>
 +
<td>
 +
<font class="BBok">I48</font>
 +
<br/>
 +
(it was I48-1)
 +
<br/><partinfo>BBa_K300088</partinfo>
 +
<br/>
 +
</td>
 +
<td>
 +
I39 (S-P) + I34 (X-P)
 +
</td>
 +
<td>
 +
31/08/2010
 +
</td><td>
 +
''E. coli'' TOP10
 +
</td>
 +
<td>
 +
<partinfo>pSB1A2</partinfo>
 +
</td>
 +
<td>
 +
Seq ok (BMR), length ok.
 +
</td>
 +
<td>
 +
2098 bp
 +
</td>
 +
<td>pTet + Pha10S + PhaSS + GFPs + TT</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td>
 +
<font class="BBok">I49</font>
 +
<br/>
 +
(it was I49-1)
 +
<br/><partinfo>BBa_K300090</partinfo>
 +
<br/>
 +
</td>
 +
<td>
 +
I39 (S-P) + I41 (X-P)
 +
</td>
 +
<td>
 +
31/08/2010
 +
</td><td>
 +
''E. coli'' TOP10
 +
</td>
 +
<td>
 +
<partinfo>pSB1A2</partinfo>
 +
</td>
 +
<td>
 +
Seq ok (BMR), length ok.
 +
</td>
 +
<td>
 +
2134 bp
 +
</td>
 +
<td>pTet + Pha10S + linker + PhaSS + GFPs + TT</td>
 +
</tr>
 +
 
 +
 
 +
<tr>
 +
<td>
 +
I50<br/>
 +
FAILED (no trasformants on plate)
 +
<br/>
 +
</td>
 +
<td>
 +
I44 (E-H) on itself
 +
</td>
 +
<td>
 +
28/08/2010
 +
</td><td>
 +
''E. coli'' DB3.1
 +
</td>
 +
<td>
 +
CREAM
 +
</td>
 +
<td>
 +
 
 +
</td>
 +
<td>
 +
3239 bp
 +
</td>
 +
<td>CREAM fragment + Cm resistance + TT + R6K ori + marker ccdB</td>
 +
</tr>
 +
 
 +
 
 +
 
 +
<tr>
 +
<td>
 +
<font class="BBok">I51</font>
 +
<br/>
 +
(it was I51-3)
 +
<br/><partinfo>BBa_I52002</partinfo>
 +
<br/>
 +
</td>
 +
<td>
 +
CREAM (E-P) <br/>+<br/><partinfo>BBa_I52002</partinfo> (E-P)
 +
</td>
 +
<td>
 +
03/09/2010
 +
</td><td>
 +
''E. coli'' DB3.1
 +
</td>
 +
<td>
 +
CREAM
 +
</td>
 +
<td>
 +
 
 +
</td>
 +
<td>
 +
3239 bp
 +
</td>
 +
<td>CREAM fragment + Cm resistance + TT + R6K ori + marker ccdB</td>
 +
</tr>
 +
 
 +
 
 +
<tr>
 +
<td>
 +
<font class="BBok">I52</font>
 +
<br/>
 +
(it was I52-c)
 +
<br/>
 +
</td>
 +
<td>
 +
I35 (S-P)<br/>+<br/>I37 (X-P)
 +
</td>
 +
<td>
 +
17/09/2010
</td><td>
</td><td>
''E. coli'' DH5-alpha
''E. coli'' DH5-alpha
 +
</td>
 +
<td>
 +
<partinfo>pSB1A2</partinfo>
 +
</td>
 +
<td>
 +
Length ok.
 +
</td>
 +
<td>
 +
1985 bp
 +
</td>
 +
<td>PhaSS + linker + INTEIN + GFPs + TT</td>
 +
</tr>
 +
 +
 +
<tr>
 +
<td>
 +
<font class="BBok">I53</font>
 +
<br/>
 +
(it was I53-c)
 +
<br/>
 +
</td>
 +
<td>
 +
I32 (E-S)<br/>+<br/>I37 (E-X)
 +
</td>
 +
<td>
 +
17/09/2010
</td><td>
</td><td>
-
<partinfo>BBa_J61002</partinfo>
+
''E. coli'' DH5-alpha
 +
</td>
 +
<td>
 +
<partinfo>pSB1AK3</partinfo>
 +
</td>
 +
<td>
 +
Length ok.
 +
</td>
 +
<td>
 +
2531 bp
 +
</td>
 +
<td>Pha10S + PhaSS + INTEIN + GFPs + TT</td>
 +
</tr>
 +
 
 +
 
 +
<tr>
 +
<td>
 +
<font class="BBok">I54</font>
 +
<br/>
 +
(it was I54-a)
 +
<br/>
 +
</td>
 +
<td>
 +
I36 (E-S)<br/>+<br/>I37 (E-X)
 +
</td>
 +
<td>
 +
17/09/2010
</td><td>
</td><td>
-
none
+
''E. coli'' DH5-alpha
 +
</td>
 +
<td>
 +
<partinfo>pSB1AK3</partinfo>
 +
</td>
 +
<td>
 +
Length ok.
</td>  
</td>  
-
<td></td><td>Ligations 2010</td>
+
<td>
 +
2531 bp
 +
</td>
 +
<td>PhaSS + PhaSS + INTEIN + GFPs + TT</td>
</tr>
</tr>
 +
<tr>
<tr>
<td>
<td>
-
I7-2<br/>
+
<font class="BBok">I55</font>
 +
<br/>
 +
(it was I55-1)
 +
<br/><partinfo>BBa_K300082</partinfo>
 +
<br/>
 +
</td>
 +
<td>
 +
I38 (E-S)<br/>+<br/>I20 (E-X)
 +
</td>
 +
<td>
 +
02/10/2010
</td><td>
</td><td>
-
<partinfo>BBa_J23118</partinfo> (E-S)<br/>+<br/>I6-2 (X-P)</td>
+
''E. coli'' DH5-alpha
 +
</td>
<td>
<td>
-
25/06/2010
+
<partinfo>pSB1AK3</partinfo>
 +
</td>
 +
<td>
 +
Seq ok (BMR), length ok.
 +
</td>
 +
<td>
 +
1663 bp
 +
</td>
 +
<td>HSL inducible system + Pha10S</td>
 +
</tr>
 +
 
 +
 
 +
 
 +
<tr>
 +
<td>
 +
<font class="BBok">I56</font>
 +
<br/>
 +
(it was I56-a)
 +
<br/>
 +
</td>
 +
<td>
 +
I26 (E-S)<br/>+<br/>I41 (E-X)
 +
</td>
 +
<td>
 +
17/09/2010
</td><td>
</td><td>
''E. coli'' DH5-alpha
''E. coli'' DH5-alpha
 +
</td>
 +
<td>
 +
<partinfo>pSB1AK3</partinfo>
 +
</td>
 +
<td>
 +
Length ok.
 +
</td>
 +
<td>
 +
2090 bp
 +
</td>
 +
<td>linker + PhaSS + linker + PhaSS + GFPs + TT</td>
 +
</tr>
 +
 +
 +
 +
<tr>
 +
<td>
 +
<font class="BBok">I57</font>
 +
<br/>
 +
(it was I57-a)
 +
<br/>
 +
</td>
 +
<td>
 +
I40 (E-S)<br/>+<br/>I0 (E-X)
 +
</td>
 +
<td>
 +
17/09/2010
</td><td>
</td><td>
-
<partinfo>BBa_J61002</partinfo>
+
''E. coli'' DH5-alpha
 +
</td>
 +
<td>
 +
<partinfo>pSB1AK3</partinfo>
 +
</td>
 +
<td>
 +
Length ok.
 +
</td>
 +
<td>
 +
1985 bp
 +
</td>
 +
<td>linker + PhaSS + INTEIN + GFPs + TT</td>
 +
</tr>
 +
 
 +
 
 +
 
 +
<tr>
 +
<td>
 +
<font class="BBok">I58</font>
 +
<br/>
 +
(it was I58-c)
 +
<br/>
 +
</td>
 +
<td>
 +
I38 (E-S)<br/>+<br/>I31 (E-X)
 +
</td>
 +
<td>
 +
17/09/2010
</td><td>
</td><td>
-
none
+
''E. coli'' DH5-alpha
 +
</td>
 +
<td>
 +
<partinfo>pSB1A2</partinfo>
 +
</td>
 +
<td>
 +
Length ok.
</td>  
</td>  
-
<td></td><td>Ligations 2010</td>
+
<td>
 +
1699 bp
 +
</td>
 +
<td>HSL inducible system + Pha10S + linker</td>
</tr>
</tr>
<tr>
<tr>
<td>
<td>
-
I8-1<br/>
+
<font class="BBok">I59</font>
 +
<br/>
 +
(it was I59-b)
 +
<br/>
 +
</td>
 +
<td>
 +
I21 (E-S)<br/> + <br/>I34 (E-X)
 +
</td>
 +
<td>
 +
17/09/2010
</td><td>
</td><td>
-
<partinfo>BBa_J23110</partinfo> (E-S)<br/>+<br/>I6-2 (X-P)</td>
+
''E. coli'' DH5-alpha
 +
</td>
<td>
<td>
-
25/06/2010
+
<partinfo>pSB1AK3</partinfo>
 +
</td>
 +
<td>
 +
Length ok.
 +
</td>
 +
<td>
 +
2018 bp
 +
</td>
 +
<td>PhaSS + PhaSS + GFPs + TT</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td>
 +
<font class="BBok">I60</font>
 +
<br/>
 +
(it was I60-a)
 +
<br/>
 +
</td>
 +
<td>
 +
I20 (S-P)<br/>+<br/>I26 (X-P)
 +
</td>
 +
<td>
 +
17/09/2010
</td><td>
</td><td>
''E. coli'' DH5-alpha
''E. coli'' DH5-alpha
 +
</td>
 +
<td>
 +
<partinfo>pSB1AK3</partinfo>
 +
</td>
 +
<td>
 +
Seq ok (BMR), length ok.
 +
</td>
 +
<td>
 +
1194 bp
 +
</td>
 +
<td>Pha10S + linker + PhaSS</td>
 +
</tr>
 +
 +
<tr>
 +
<td>
 +
<font class="BBok">I61</font>
 +
<br/>
 +
(it was I61-a)
 +
<br/>
 +
</td>
 +
<td>
 +
I31 (E-S)<br/>+<br/>I37 (E-X)
 +
</td>
 +
<td>
 +
17/09/2010
</td><td>
</td><td>
-
<partinfo>BBa_J61002</partinfo>
+
''E. coli'' DH5-alpha
 +
</td>
 +
<td>
 +
<partinfo>pSB1AK3</partinfo>
 +
</td>
 +
<td>
 +
Length ok.
 +
</td>
 +
<td>
 +
1985 bp
 +
</td>
 +
<td>Pha10S + linker + INTEIN + GFPs + TT</td>
 +
</tr>
 +
 
 +
 
 +
<tr>
 +
<td>
 +
<font class="BBok">I62</font>
 +
<br/>
 +
(it was I62-E)
 +
<br/>
 +
</td>
 +
<td>
 +
I39 (S-P)<br/>+<br/>I59 (X-P)
 +
</td>
 +
<td>
 +
27/09/2010
</td><td>
</td><td>
-
none
+
''E. coli'' TOP10
 +
</td>
 +
<td>
 +
<partinfo>pSB1A2</partinfo>
 +
</td>
 +
<td>
 +
Length ok.
</td>  
</td>  
-
<td></td><td>Ligations 2010</td>
+
<td>
 +
2678 bp
 +
</td>
 +
<td>pTet + Pha10S + PhaSS + PhaSS + GFPs + TT</td>
</tr>
</tr>
 +
<tr>
<tr>
<td>
<td>
-
I8-2<br/>
+
<font class="BBok">I63</font>
 +
<br/>
 +
(it was I63-1)
 +
<br/><partinfo>BBa_K300091</partinfo>
 +
<br/>
 +
</td>
 +
<td>
 +
I38 (E-S)<br/>+<br/>I61 (E-X)
 +
</td>
 +
<td>
 +
24/09/2010
</td><td>
</td><td>
-
<partinfo>BBa_J23110</partinfo> (E-S)<br/>+<br/>I6-2 (X-P)</td>
+
''E. coli'' TOP10
 +
</td>
<td>
<td>
-
25/06/2010
+
<partinfo>pSB1AK3</partinfo>
 +
</td>
 +
<td>
 +
Length ok.
 +
</td>
 +
<td>
 +
3072 bp
 +
</td>
 +
<td>HSL inducible system + Pha10S + linker + INTEIN + GFPs + TT</td>
 +
</tr>
 +
 
 +
 
 +
<tr>
 +
<td>
 +
<font class="BBok">I64</font>
 +
<br/>
 +
(it was I64-2)
 +
<br/><partinfo>BBa_K300092</partinfo>
 +
<br/>
 +
</td>
 +
<td>
 +
I58 (E-S)<br/>+<br/>I52 (E-X)
 +
</td>
 +
<td>
 +
24/09/2010
 +
</td><td>
 +
''E. coli'' TOP10
 +
</td>
 +
<td>
 +
<partinfo>pSB1A2</partinfo>
 +
</td>
 +
<td>
 +
Length ok.
 +
</td>
 +
<td>
 +
3690 bp
 +
</td>
 +
<td>HSL inducible system + Pha10S + linker + PhaSS + linker + INTEIN + GFPs + TT</td>
 +
</tr>
 +
 
 +
 
 +
<tr>
 +
<td>
 +
<font class="BBok">I65</font>
 +
<br/>
 +
(it was I65-3)
 +
<br/><partinfo>BBa_K300099</partinfo>
 +
<br/>
 +
</td>
 +
<td>
 +
I39 (S-P)<br/>+<br/>I56 (X-P)
 +
</td>
 +
<td>
 +
24/09/2010
 +
</td><td>
 +
''E. coli'' TOP10
 +
</td>
 +
<td>
 +
<partinfo>pSB1A2</partinfo>
 +
</td>
 +
<td>
 +
Length ok.
 +
</td>
 +
<td>
 +
2752 bp
 +
</td>
 +
<td>pTet + Pha10S + linker + PhaSS + linker + PhaSS + GFPs + TT</td>
 +
</tr>
 +
 
 +
 
 +
<tr>
 +
<td>
 +
<font class="BBok">I66</font>
 +
<br/>
 +
(it was I66-1)
 +
<br/>
 +
</td>
 +
<td>
 +
I60 (E-S)<br/>+<br/>I57 (E-X)
 +
</td>
 +
<td>
 +
24/09/2010
 +
</td><td>
 +
''E. coli'' TOP10
 +
</td>
 +
<td>
 +
<partinfo>pSB1AK3</partinfo>
 +
</td>
 +
<td>
 +
Length ok.
 +
</td>
 +
<td>
 +
3185 bp
 +
</td>
 +
<td>Pha10S + linker + PhaSS + linker + PhaSS + INTEIN + GFPs + TT</td>
 +
</tr>
 +
 
 +
 
 +
<tr>
 +
<td>
 +
<font class="BBok">I70</font>
 +
<br/>
 +
(it was I70-1)
 +
<br/><partinfo>BBa_K300007</partinfo>
 +
<br/>
 +
</td>
 +
<td>
 +
MyYeast3 (E-S)<br/>+<br/>I4 (E-S)
 +
</td>
 +
<td>
 +
01/10/10
 +
</td><td>
 +
''E. coli'' TOP10
 +
</td>
 +
<td>
 +
MyYeast (Amp100 in coli)
 +
</td>
 +
<td>
 +
 
 +
</td>
 +
<td>
 +
5187bp
 +
</td>
 +
<td>MyYeast vector + I4 in cloning site (Ptef1 + mOrange + Tadh1)</td>
 +
</tr>
 +
 
 +
 
 +
 
 +
<tr>
 +
<td>
 +
<font class="BBok">I71</font>
 +
<br/>
 +
(it was I71-1)
 +
</td>
 +
<td>
 +
MyYeast3 (E-S)<br/>+<br/>I1 (E-S)
 +
</td>
 +
<td>
 +
01/10/10
 +
</td><td>
 +
''E. coli'' TOP10
 +
</td>
 +
<td>
 +
MyYeast (Amp100 in coli)
 +
</td>
 +
<td>
 +
 
 +
</td>
 +
<td>
 +
4778bp
 +
</td>
 +
<td>MyYeast vector + I1 in cloning site (mOrange + Tadh1, NO promoter)</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td>
 +
I72<br/>
 +
(it was I70-x)
 +
</td>
 +
<td>
 +
<partinfo>BBa_J04450</partinfo>(E-P)+<partinfo>pSB4C5</partinfo>(E-P)</td>
 +
<td>
 +
 
 +
</td><td>
 +
''E. coli'' TOP10
 +
</td>
 +
<td>
 +
<partinfo>pSB4C5</partinfo>
 +
</td>
 +
<td>
 +
 
 +
</td>
 +
<td>
 +
4290bp
 +
</td>
 +
<td>mRFP under pLac promoter in LC plasmid pSB4C5</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td>
 +
<font class="BBok">I73</font>
 +
<br/>
 +
(it was I73-1)
 +
<br/>
 +
</td>
 +
<td>
 +
I38 (E-S)<br/>+<br/>I53 (E-X)
 +
</td>
 +
<td>
 +
02/10/2010
 +
</td><td>
 +
''E. coli'' TOP10
 +
</td>
 +
<td>
 +
<partinfo>pSB1AK3</partinfo>
 +
</td>
 +
<td>
 +
Length ok.
 +
</td>
 +
<td>
 +
3618 bp
 +
</td>
 +
<td>HSL inducible system + Pha10S + PhaSS + INTEIN + GFPs + TT</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td>
 +
<font class="BBok">I74</font>
 +
<br/>
 +
(it was I74-8)
 +
<br/>
 +
</td>
 +
<td>
 +
I31 (E-S)<br/>+<br/>I35 (E-X)
 +
</td>
 +
<td>
 +
04/10/2010
 +
</td><td>
 +
''E. coli'' TOP10
 +
</td>
 +
<td>
 +
<partinfo>pSB1A2</partinfo>
 +
</td>
 +
<td>
 +
Length ok.
 +
</td>
 +
<td>
 +
1230 bp
 +
</td>
 +
<td>Pha10S + linker + PhaSS + linker</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td>
 +
I75<br/>
 +
(it was I75-x)
 +
</td>
 +
<td>
 +
I55 (E-S)<br/>+<br/>I37 (E-X)
 +
</td>
 +
<td>
 +
Not screened yet.
 +
</td><td>
 +
''E. coli'' TOP10
 +
</td>
 +
<td>
 +
<partinfo>pSB1AK3</partinfo>
 +
</td>
 +
<td>
 +
Not screened yet.
 +
</td>
 +
<td>
 +
3036 bp
 +
</td>
 +
<td>HSL inducible system + Pha10S + INTEIN + GFPs + TT</td>
 +
</tr>
 +
 
 +
 
 +
<tr>
 +
<td>
 +
I76<br/>
 +
(it was I76-x)
 +
</td>
 +
<td>
 +
I55 (E-S)<br/>+<br/>I54 (E-X)
 +
</td>
 +
<td>
 +
Not screened yet.
</td><td>
</td><td>
''E. coli'' DH5-alpha
''E. coli'' DH5-alpha
 +
</td>
 +
<td>
 +
<partinfo>pSB1AK3</partinfo>
 +
</td>
 +
<td>
 +
Not screened yet.
 +
</td>
 +
<td>
 +
4191 bp
 +
</td>
 +
<td>HSL inducible system + Pha10S + PhaSS + PhaSS + INTEIN + GFPs + TT</td>
 +
</tr>
 +
 +
 +
<tr>
 +
<td>
 +
I77<br/>
 +
(it was I77-x)
 +
</td>
 +
<td>
 +
I55 (E-S)<br/>+<br/>I57 (E-X)
 +
</td>
 +
<td>
 +
Not screened yet.
</td><td>
</td><td>
-
<partinfo>BBa_J61002</partinfo>
+
''E. coli'' TOP10
 +
</td>
 +
<td>
 +
<partinfo>pSB1AK3</partinfo>
 +
</td>
 +
<td>
 +
Not screened yet.
 +
</td>
 +
<td>
 +
3654 bp
 +
</td>
 +
<td>HSL inducible system + Pha10S + linker + PhaSS + INTEIN + GFPs + TT</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td>
 +
<font class="BBok">I78</font>
 +
<br/>
 +
(it was I78-3)
 +
<br/><partinfo>BBa_K300084</partinfo>
 +
<br/>
 +
</td>
 +
<td>
 +
I74 (E-S)<br/>+<br/>INTEIN_C3 (E-X)
 +
</td>
 +
<td>
 +
07/10/10
</td><td>
</td><td>
-
none
+
''E. coli'' TOP10
 +
</td>
 +
<td>
 +
<partinfo>pSB1C3</partinfo>
 +
</td>
 +
<td>
 +
Seq ok (BMR), length ok.
</td>  
</td>  
-
<td></td><td>Ligations 2010</td>
+
<td>
 +
1743 bp
 +
</td>
 +
<td>Pha10S + linker + PhaSS + linker + INTEIN</td>
</tr>
</tr>
<tr>
<tr>
<td>
<td>
-
I9-1<br/>
+
<font class="BBok">I79</font>
 +
<br/>
 +
(it was I79-2)
 +
<br/><partinfo>BBa_K300083</partinfo>
 +
<br/>
 +
</td>
 +
<td>
 +
I60 (E-S)<br/>+<br/>INTEIN_C3 (E-X)
 +
</td>
 +
<td>
 +
07/10/10
</td><td>
</td><td>
-
<partinfo>BBa_J23111</partinfo> (E-S)<br/>+<br/>I6-2 (X-P)</td>
+
''E. coli'' STBL3
 +
</td>
<td>
<td>
-
25/06/2010
+
<partinfo>pSB1C3</partinfo>
 +
</td>
 +
<td>
 +
Seq ok (BMR), length ok.
 +
</td>
 +
<td>
 +
1707 bp
 +
</td>
 +
<td>Pha10S + linker + PhaSS + INTEIN</td>
 +
</tr>
 +
 
 +
 
 +
<tr>
 +
<td>
 +
<font class="BBok">I80</font>
 +
<br/>
 +
(it was I80-4)
 +
<br/><partinfo>BBa_K300010</partinfo>
 +
<br/>
 +
</td>
 +
<td>
 +
I3_1C3 (S-P)<br/>+<br/><partinfo>BBa_F2620</partinfo> (X-P)
 +
</td>
 +
<td>
 +
11/10/10
 +
</td><td>
 +
''E. coli'' TOP10
 +
</td>
 +
<td>
 +
<partinfo>pSB1C3</partinfo>
 +
</td>
 +
<td>
 +
Seq ok (BMR), length ok.
 +
</td>
 +
<td>
 +
1707 bp
 +
</td>
 +
<td>
 +
RBS + luxI + TT + <partinfo>BBa_F2620</partinfo>
 +
</td>
 +
</tr>
 +
 
 +
 
 +
<tr>
 +
<td>
 +
<font class="BBok">INTEIN</font>
 +
<br/><partinfo>BBa_K300004</partinfo>
 +
</td>
 +
<td>
 +
Intein
 +
</td>
 +
<td>
 +
13/08/2010
 +
</td><td>
 +
''E. coli'' TOP10
 +
</td>
 +
<td>
 +
pMA <br/> (HC Amp resistance)
 +
</td>
 +
<td>
 +
Mr.Gene DNA synthesis
 +
</td>
 +
<td>
 +
507 bp
 +
</td>
 +
<td>INTEIN</td>
 +
</tr>
 +
 
 +
 
 +
<tr>
 +
<td>
 +
<font class="BBok">INTEIN_C3</font>
 +
</td>
 +
<td>
 +
Intein
 +
</td>
 +
<td>
 +
02/10/2010
 +
</td>
 +
<td>
 +
''E. coli'' TOP10
 +
</td>
 +
<td>
 +
<partinfo>pSB1C3</partinfo>
 +
</td>
 +
<td>
 +
Mr.Gene DNA synthesis
 +
</td>
 +
<td>
 +
507 bp
 +
</td>
 +
<td>
 +
INTEIN in <partinfo>pSB1C3</partinfo>
 +
</td>
 +
</tr>
 +
 
 +
 
 +
 
 +
<tr>
 +
<td>
 +
<font class="BBok">YEAST<br/>MR.G</font>
 +
<br/><partinfo>BBa_K300980</partinfo>
 +
</td>
 +
<td>
 +
YEAST fragment
 +
</td>
 +
<td>
 +
15/09/2010
</td><td>
</td><td>
''E. coli'' DH5-alpha
''E. coli'' DH5-alpha
 +
</td>
 +
<td>
 +
pMA <br/> (HC Amp resistance)
 +
</td>
 +
<td>
 +
Mr.Gene DNA synthesis
 +
</td>
 +
<td>
 +
1695 bp
 +
</td>
 +
<td>YEAST fragment</td>
 +
</tr>
 +
 +
 +
<tr>
 +
<td>
 +
<font class="BBok">CRIM<br/>MR.G</font>
 +
<br/><partinfo>BBa_K300983</partinfo>
 +
</td>
 +
<td>
 +
CREAM fragment
 +
</td>
 +
<td>
 +
13/08/2010
</td><td>
</td><td>
-
<partinfo>BBa_J61002</partinfo>
+
''E. coli'' TOP10
 +
</td>
 +
<td>
 +
pMK-RQ <br/> (HC Kan resistance)
 +
</td>
 +
<td>
 +
Mr.Gene DNA synthesis
 +
</td>
 +
<td>
 +
829 bp
 +
</td>
 +
<td>CREAM fragment</td>
 +
</tr>
 +
 
 +
 
 +
<tr>
 +
<td>
 +
<font class="BBok">CREAM</font>
 +
<br/>
 +
(it was MyCRIM)
 +
<br/><partinfo>BBa_K300982</partinfo>
 +
<br/>
 +
</td>
 +
<td>
 +
CRIM<br/>MR.G (A-A) <br/>+<br/>I5 (S-X)
 +
</td>
 +
<td>
 +
21/08/2010
</td><td>
</td><td>
-
none
+
''E. coli'' BW23474
 +
</td>
 +
<td>
 +
CREAM
 +
</td>
 +
<td>
 +
Length ok.
</td>  
</td>  
-
<td></td><td>Ligations 2010</td>
+
<td>
 +
2149 bp
 +
</td>
 +
<td>CREAM fragment + Cm resistance + TT + R6K ori</td>
</tr>
</tr>
 +
<tr>
<tr>
<td>
<td>
-
I9-2<br/>
+
<font class="BBok">I7-4C5</font>
 +
<br/>
 +
(it was I7-4C5-2)
 +
</td>
 +
<td>
 +
I7 (E-P)<br/>+<br/> <partinfo>pSB4C5</partinfo>(E-P)
 +
</td>
 +
<td>
 +
16/07/2010
</td><td>
</td><td>
-
<partinfo>BBa_J23111</partinfo> (E-S)<br/>+<br/>I6-2 (X-P)</td>
+
''E. coli'' TOP10
 +
</td><td>
 +
<partinfo>pSB4C5</partinfo>
 +
</td><td>
 +
Seq ok (BMR), length ok.
 +
</td>
 +
<td>2795 bp</td>
 +
<td>constitutive promoter + RBS + luxI + TT + HSL sensor in <partinfo>pSB4C5</partinfo></td>
 +
</tr>
 +
 
 +
 
 +
<tr>
<td>
<td>
-
25/06/2010
+
<font class="BBok">I8-4C5</font>
 +
<br/>
 +
(it was I8-4C5-2)
 +
</td>
 +
<td>
 +
I8 (E-P)<br/>+<br/><partinfo>pSB4C5</partinfo> (E-P)
 +
</td>
 +
<td>
 +
16/07/2010
 +
</td><td>
 +
''E. coli'' TOP10
 +
</td><td>
 +
<partinfo>pSB4C5</partinfo>
 +
</td><td>
 +
Seq ok (BMR), length ok.
 +
</td>
 +
<td>2795 bp</td>
 +
<td>constitutive promoter + RBS + luxI + TT + HSL sensor in <partinfo>pSB4C5</partinfo></td>
 +
</tr>
 +
 
 +
<tr>
 +
<td>
 +
<font class="BBok">I10-4C5</font>
 +
<br/>
 +
(it was I10-4C5-1)
 +
</td>
 +
<td>
 +
I10 (E-P)<br/>+<br/><partinfo>pSB4C5</partinfo> (E-P)
 +
</td>
 +
<td>
 +
16/07/2010
 +
</td><td>
 +
''E. coli'' TOP10
 +
</td><td>
 +
<partinfo>pSB4C5</partinfo>
 +
</td><td>
 +
Length ok.
 +
</td>
 +
<td>
 +
2795 bp
 +
</td>
 +
<td>constitutive promoter + RBS + luxI + TT + HSL sensor in <partinfo>pSB4C5</partinfo></td>
 +
</tr>
 +
 
 +
 
 +
<tr>
 +
<td>
 +
<font class="BBok">I12-4C5</font>
 +
<br/>
 +
(it was I12-4C5-1)
 +
</td>
 +
<td>
 +
I12 (E-P)<br/>+<br/><partinfo>pSB4C5</partinfo> (E-P)
 +
</td>
 +
<td>
 +
25/07/2010
 +
</td><td>
 +
''E. coli'' TOP10
 +
</td><td>
 +
<partinfo>pSB4C5</partinfo>
 +
</td><td>
 +
Length ok.
 +
</td>
 +
<td>
 +
2795 bp
 +
</td>
 +
<td>constitutive promoter + RBS + luxI + TT + HSL sensor in <partinfo>pSB4C5</partinfo></td>
 +
</tr>
 +
 
 +
 
 +
<tr>
 +
<td>
 +
<font class="BBok">I14-4C5</font>
 +
<br/>
 +
(it was I14-4C5-2)
 +
</td>
 +
<td>
 +
I14 (E-P)<br/>+<br/><partinfo>pSB4C5</partinfo> (E-P)
 +
</td>
 +
<td>
 +
23/07/2010
</td><td>
</td><td>
''E. coli'' DH5-alpha
''E. coli'' DH5-alpha
</td><td>
</td><td>
-
<partinfo>BBa_J61002</partinfo>
+
<partinfo>pSB4C5</partinfo>
</td><td>
</td><td>
-
none
+
Length ok.
</td>  
</td>  
-
<td></td><td>Ligations 2010</td>
+
<td>
 +
842 bp
 +
</td>
 +
<td>constitutive promoter + RBS + luxI + TT + HSL sensor in <partinfo>pSB4C5</partinfo></td>
</tr>
</tr>
<tr>
<tr>
<td>
<td>
-
I10-1<br/>
+
<font class="BBok">I15-4C5</font>
 +
<br/>
 +
(it was I15-4C5-2)
 +
</td>
 +
<td>
 +
I15 (E-P)<br/>+<br/><partinfo>pSB4C5</partinfo> (E-P)
 +
</td>
 +
<td>
 +
13/08/2010
</td><td>
</td><td>
-
<partinfo>BBa_J23116</partinfo> (E-S)<br/>+<br/>I6-2 (X-P)</td>
+
''E. coli'' TOP10
 +
</td><td>
 +
<partinfo>pSB4C5</partinfo>
 +
</td><td>
 +
Length ok.
 +
</td>  
<td>
<td>
-
25/06/2010
+
842 bp
 +
</td>
 +
<td>constitutive promoter + RBS + luxI + TT in <partinfo>pSB4C5</partinfo></td>
 +
</tr>
 +
 
 +
 
 +
<tr>
 +
<td>
 +
<font class="BBok">I16-4C5</font>
 +
<br/>
 +
(it was I16-4C5-1)
 +
</td>
 +
<td>
 +
I16 (E-P)<br/>+<br/><partinfo>pSB4C5</partinfo> (E-P)
 +
</td>
 +
<td>
 +
23/07/2010
</td><td>
</td><td>
''E. coli'' DH5-alpha
''E. coli'' DH5-alpha
</td><td>
</td><td>
-
<partinfo>BBa_J61002</partinfo>
+
<partinfo>pSB4C5</partinfo>
</td><td>
</td><td>
-
none
+
Length ok.
</td>  
</td>  
-
<td></td><td>Ligations 2010</td>
+
<td>
 +
842 bp
 +
</td>
 +
<td>constitutive promoter + RBS + luxI + TT in <partinfo>pSB4C5</partinfo></td>
</tr>
</tr>
 +
 +
 +
<tr>
<tr>
<td>
<td>
-
I10-2<br/>
+
<font class="BBok">I17-4C5</font>
 +
<br/>
 +
(it was I17-4C5-1)
 +
</td>
 +
<td>
 +
I17 (E-P)<br/>+<br/><partinfo>pSB4C5</partinfo> (E-P)
 +
</td>
 +
<td>
 +
25/07/2010
</td><td>
</td><td>
-
<partinfo>BBa_J23116</partinfo> (E-S)<br/>+<br/>I6-2 (X-P)</td>
+
''E. coli'' DH5-alpha
 +
</td><td>
 +
<partinfo>pSB4C5</partinfo>
 +
</td><td>
 +
Length ok.
 +
</td>  
<td>
<td>
-
25/06/2010
+
842 bp
 +
</td>
 +
<td>constitutive promoter + RBS + luxI + TT in <partinfo>pSB4C5</partinfo></td>
 +
</tr>
 +
 
 +
 
 +
<tr>
 +
<td>
 +
<font class="BBok">I18-4C5</font>
 +
<br/>
 +
(it was I18-4C5-1)
 +
</td>
 +
<td>
 +
I18 (E-P)<br/>+<br/><partinfo>pSB4C5</partinfo> (E-P)
 +
</td>
 +
<td>
 +
25/07/2010
</td><td>
</td><td>
''E. coli'' DH5-alpha
''E. coli'' DH5-alpha
</td><td>
</td><td>
-
<partinfo>BBa_J61002</partinfo>
+
<partinfo>pSB4C5</partinfo>
</td><td>
</td><td>
-
none
+
Length ok.
</td>  
</td>  
-
<td></td><td>Ligations 2010</td>
+
<td>
 +
842 bp
 +
</td>
 +
<td>constitutive promoter + RBS + luxI + TT in <partinfo>pSB4C5</partinfo></td>
</tr>
</tr>
 +
 +
 +
<tr>
 +
<td>
 +
<font class="BBok">I19-4C5</font>
 +
<br/>
 +
(it was I19-4C5-1)
 +
</td>
 +
<td>
 +
I19 (E-P)<br/>+<br/><partinfo>pSB4C5</partinfo> (E-P)
 +
</td>
 +
<td>
 +
25/07/2010
 +
</td><td>
 +
''E. coli'' DH5-alpha
 +
</td><td>
 +
<partinfo>pSB4C5</partinfo>
 +
</td><td>
 +
Length ok.
 +
</td>
 +
<td>
 +
842 bp
 +
</td>
 +
<td>constitutive promoter + RBS + luxI + TT in <partinfo>pSB4C5</partinfo></td>
 +
</tr>
 +
 +
 +
 +
<tr>
 +
<td>
 +
I27
 +
<br/>
 +
</td>
 +
<td>
 +
I23 (E-P)<br/>+<br/><partinfo>pSB4C5</partinfo> (E-P)
 +
</td>
 +
<td>
 +
17/08/2010
 +
</td><td>
 +
''E. coli'' TOP10
 +
</td><td>
 +
<partinfo>pSB4C5</partinfo>
 +
</td><td>
 +
 +
</td>
 +
<td>
 +
725 bp
 +
</td>
 +
<td>plux + RBS + luxI + TT in <partinfo>pSB4C5</partinfo></td>
 +
</tr>
 +
 +
 +
<tr>
 +
<td>
 +
I28
 +
</td>
 +
<td>
 +
I24 (E-P)<br/>+<br/><partinfo>pSB4C5</partinfo> (E-P)
 +
</td>
 +
<td>
 +
17/08/2010
 +
</td><td>
 +
''E. coli'' TOP10
 +
</td><td>
 +
<partinfo>pSB4C5</partinfo>
 +
</td><td>
 +
 +
</td>
 +
<td>
 +
886 bp
 +
</td>
 +
<td>plux + RBS + lasI + terminator in <partinfo>pSB4C5</partinfo></td>
 +
</tr>
 +
 +
 +
<tr>
 +
<td>
 +
<font class="BBok">BBa_J23100<br/>_4C5</font>
 +
<br/>
 +
(it was <partinfo>BBa_J23100</partinfo><br/>_4C5-1)
 +
</td>
 +
<td>
 +
<partinfo>BBa_J23100</partinfo> <br/>+<br/> <partinfo>BBa_B0034</partinfo>  <br/>+<br/> <partinfo>BBa_E1010</partinfo>  <br/>+<br/> <partinfo>BBa_B0015</partinfo> in <partinfo>pSB4C5</partinfo>.
 +
</td>
 +
<td>
 +
27/08/2010
 +
</td><td>
 +
''E. coli'' TOP10
 +
</td><td>
 +
<partinfo>pSB4C5</partinfo>
 +
</td><td>
 +
Seq ok (BMR), length ok.
 +
</td>
 +
<td>
 +
904 bp
 +
</td>
 +
<td>constitutive promoter + RBS + mRFP1 + TT in <partinfo>pSB4C5</partinfo></td>
 +
</tr>
 +
 +
<tr>
 +
<td>
 +
<font class="BBok">BBa_J23101<br/>_4C5</font>
 +
<br/>
 +
(it was <partinfo>BBa_J23101</partinfo><br/>_4C5-1)
 +
</td>
 +
<td>
 +
<partinfo>BBa_J23101</partinfo> <br/>+<br/> <partinfo>BBa_B0034</partinfo>  <br/>+<br/> <partinfo>BBa_E1010</partinfo>  <br/>+<br/> <partinfo>BBa_B0015</partinfo> in <partinfo>pSB4C5</partinfo>.
 +
</td>
 +
<td>
 +
27/08/2010
 +
</td><td>
 +
''E. coli'' TOP10
 +
</td><td>
 +
<partinfo>pSB4C5</partinfo>
 +
</td><td>
 +
Seq ok (BMR), length ok.
 +
</td>
 +
<td>
 +
904 bp
 +
</td>
 +
<td>constitutive promoter + RBS + mRFP1 + TT in <partinfo>pSB4C5</partinfo></td>
 +
</tr>
 +
 +
<tr>
 +
<td>
 +
<font class="BBok">BBa_J23105<br/>_4C5</font>
 +
<br/>
 +
(it was <partinfo>BBa_J23105</partinfo><br/>_4C5-1)
 +
</td>
 +
<td>
 +
<partinfo>BBa_J23105</partinfo> <br/>+<br/> <partinfo>BBa_B0034</partinfo>  <br/>+<br/> <partinfo>BBa_E1010</partinfo>  <br/>+<br/> <partinfo>BBa_B0015</partinfo> in <partinfo>pSB4C5</partinfo>.
 +
</td>
 +
<td>
 +
27/08/2010
 +
</td><td>
 +
''E. coli'' TOP10
 +
</td><td>
 +
<partinfo>pSB4C5</partinfo>
 +
</td><td>
 +
Length ok.
 +
</td>
 +
<td>
 +
904 bp
 +
</td>
 +
<td>constitutive promoter + RBS + mRFP1 + TT in <partinfo>pSB4C5</partinfo></td>
 +
</tr>
 +
 +
 +
<tr>
 +
<td>
 +
<font class="BBok">BBa_J23106<br/>_4C5</font>
 +
<br/>
 +
(it was <partinfo>BBa_J23106</partinfo><br/>_4C5-1)
 +
</td>
 +
<td>
 +
<partinfo>BBa_J23106</partinfo> <br/>+<br/> <partinfo>BBa_B0034</partinfo>  <br/>+<br/> <partinfo>BBa_E1010</partinfo>  <br/>+<br/> <partinfo>BBa_B0015</partinfo> in <partinfo>pSB4C5</partinfo>.
 +
</td>
 +
<td>
 +
27/08/2010
 +
</td><td>
 +
''E. coli'' TOP10
 +
</td><td>
 +
<partinfo>pSB4C5</partinfo>
 +
</td><td>
 +
Length ok.
 +
</td>
 +
<td>
 +
904 bp
 +
</td>
 +
<td>constitutive promoter + RBS + mRFP1 + TT in <partinfo>pSB4C5</partinfo></td>
 +
</tr>
 +
 +
<tr>
 +
<td>
 +
<font class="BBok">BBa_J23110<br/>_4C5</font>
 +
<br/>
 +
(it was <partinfo>BBa_J23110</partinfo><br/>_4C5-1)
 +
</td>
 +
<td>
 +
<partinfo>BBa_J23110</partinfo> <br/>+<br/> <partinfo>BBa_B0034</partinfo>  <br/>+<br/> <partinfo>BBa_E1010</partinfo>  <br/>+<br/> <partinfo>BBa_B0015</partinfo> in <partinfo>pSB4C5</partinfo>.
 +
</td>
 +
<td>
 +
27/08/2010
 +
</td><td>
 +
''E. coli'' TOP10
 +
</td><td>
 +
<partinfo>pSB4C5</partinfo>
 +
</td><td>
 +
Length ok.
 +
</td>
 +
<td>
 +
904 bp
 +
</td>
 +
<td>constitutive promoter + RBS + mRFP1 + TT in <partinfo>pSB4C5</partinfo></td>
 +
</tr>
 +
 +
<tr>
 +
<td>
 +
<font class="BBok">BBa_J23114<br/>_4C5</font>
 +
<br/>
 +
(it was <partinfo>BBa_J23114</partinfo><br/>_4C5-1)
 +
</td>
 +
<td>
 +
<partinfo>BBa_J23114</partinfo> <br/>+<br/> <partinfo>BBa_B0034</partinfo>  <br/>+<br/> <partinfo>BBa_E1010</partinfo>  <br/>+<br/> <partinfo>BBa_B0015</partinfo> in <partinfo>pSB4C5</partinfo>.
 +
</td>
 +
<td>
 +
27/08/2010
 +
</td><td>
 +
''E. coli'' TOP10
 +
</td><td>
 +
<partinfo>pSB4C5</partinfo>
 +
</td><td>
 +
Length ok.
 +
</td>
 +
<td>
 +
904 bp
 +
</td>
 +
<td>constitutive promoter + RBS + mRFP1 + TT in <partinfo>pSB4C5</partinfo></td>
 +
</tr>
 +
 +
<tr>
 +
<td>
 +
<font class="BBok">BBa_J23116<br/>_4C5</font>
 +
<br/>
 +
(it was <partinfo>BBa_J23116</partinfo><br/>_4C5-1)
 +
</td>
 +
<td>
 +
<partinfo>BBa_J23116</partinfo> <br/>+<br/> <partinfo>BBa_B0034</partinfo>  <br/>+<br/> <partinfo>BBa_E1010</partinfo>  <br/>+<br/> <partinfo>BBa_B0015</partinfo> in <partinfo>pSB4C5</partinfo>.
 +
</td>
 +
<td>
 +
30/08/2010
 +
</td><td>
 +
''E. coli'' TOP10
 +
</td><td>
 +
<partinfo>pSB4C5</partinfo>
 +
</td><td>
 +
Length ok.
 +
</td>
 +
<td>
 +
904 bp
 +
</td>
 +
<td>constitutive promoter + RBS + mRFP1 + TT in <partinfo>pSB4C5</partinfo></td>
 +
</tr>
 +
 +
 +
 +
<tr>
 +
<td>
 +
<font class="BBok">BBa_J23118<br/>_4C5</font>
 +
<br/>
 +
(it was <partinfo>BBa_J23118</partinfo><br/>_4C5-1)
 +
</td>
 +
<td>
 +
<partinfo>BBa_J23118</partinfo> <br/>+<br/> <partinfo>BBa_B0034</partinfo>  <br/>+<br/> <partinfo>BBa_E1010</partinfo>  <br/>+<br/> <partinfo>BBa_B0015</partinfo> in <partinfo>pSB4C5</partinfo>.
 +
</td>
 +
<td>
 +
30/08/2010
 +
</td><td>
 +
''E. coli'' TOP10
 +
</td><td>
 +
<partinfo>pSB4C5</partinfo>
 +
</td><td>
 +
Seq ok (BMR), length ok.
 +
</td>
 +
<td>
 +
904 bp
 +
</td>
 +
<td>constitutive promoter + RBS + mRFP1 + TT in <partinfo>pSB4C5</partinfo></td>
 +
</tr>
 +
 +
 +
 +
 +
<tr><td colspan="8"> </td></tr>
 +
<!-- Ceppi ordinati -->
 +
<tr>
 +
<th>Strain name</th>
 +
<th>Description</th>
 +
<th>Arrival date</th>
 +
<th>Infos</th>
 +
<th>Resistance</th>
 +
<th>Quality control</th>
 +
<th>Notes</th>
 +
<th>Store</th>
 +
</tr>
 +
 +
<tr>
 +
<td>
 +
MG1655(Seq)<br/>(MG)
 +
</td>
 +
<td>
 +
''E. coli'' wild type strain fully sequenced
 +
</td>
 +
<td>
 +
28/06/2010
 +
</td>
 +
<td>
 +
<html><a href="http://cgsc.biology.yale.edu/Strain.php?ID=68076" target="_blank">link</a></html>
 +
</td>
 +
<td>-</td>
 +
<td></td>
 +
<td>Genome fully sequenced</td>
 +
<td>Strains 2010</td>
 +
</tr>
 +
 +
<tr>
 +
<td>
 +
MC1061<br/>(MC)
 +
</td>
 +
<td>
 +
''E. coli'' strain
 +
</td>
 +
<td>
 +
28/06/2010
 +
</td>
 +
<td>
 +
<html><a href="http://cgsc.biology.yale.edu/Strain.php?ID=11225" target="_blank">link</a></html>
 +
</td>
 +
<td>-</td>
 +
<td></td>
 +
<td>Strain used by JC Anderson et al. for integration</td>
 +
<td>Strains 2010</td>
 +
</tr>
 +
 +
<tr>
 +
<td>
 +
BW25141 <br/>(BW1)
 +
</td>
 +
<td>
 +
''E. coli'' pir+ strain
 +
</td>
 +
<td>
 +
28/06/2010
 +
</td>
 +
<td>
 +
<html><a href="http://cgsc.biology.yale.edu/Strain.php?ID=64690" target="_blank">link</a></html>
 +
</td>
 +
<td>-</td>
 +
<td>Actually replicates the conditional replication origin R6K</td>
 +
<td>LC pir</td>
 +
<td>Strains 2010</td>
 +
</tr>
 +
 +
<tr>
 +
<td>
 +
BW25142 <br/>(BW2)
 +
</td>
 +
<td>
 +
''E. coli'' pir116 strain
 +
</td>
 +
<td>
 +
28/06/2010
 +
</td>
 +
<td>
 +
<html><a href="http://cgsc.biology.yale.edu/Strain.php?ID=70715" target="_blank">link</a></html>
 +
</td>
 +
<td>-</td>
 +
<td>Actually replicates the conditional replication origin R6K</td>
 +
<td>HC pir</td>
 +
<td>Strains 2010</td>
 +
</tr>
 +
 +
<tr>
 +
<td>
 +
BW23473
 +
</td>
 +
<td>
 +
''E. coli'' pir+ strain
 +
</td>
 +
<td>
 +
19/07/2010
 +
</td>
 +
<td>
 +
<html><a href="http://cgsc.biology.yale.edu/Strain.php?ID=70679" target="_blank">link</a></html>
 +
</td>
 +
<td>-</td>
 +
<td></td>
 +
<td>LC pir</td>
 +
<td>Strains 2010</td>
 +
</tr>
 +
 +
<tr>
 +
<td>
 +
BW23474
 +
</td>
 +
<td>
 +
''E. coli'' pir116 strain
 +
</td>
 +
<td>
 +
28/06/2010
 +
</td>
 +
<td>
 +
<html><a href="http://cgsc.biology.yale.edu/Strain.php?ID=70692" target="_blank">link</a></html>
 +
</td>
 +
<td>-</td>
 +
<td>Actually replicates the conditional replication origin R6K</td>
 +
<td>HC pir</td>
 +
<td>Strains 2010</td>
 +
</tr>
 +
 +
<tr>
 +
<td>
 +
BW5328/pAH123
 +
</td>
 +
<td>
 +
''E. coli'' strain
 +
</td>
 +
<td>
 +
28/06/2010
 +
</td>
 +
<td>
 +
<html><a href="http://cgsc.biology.yale.edu/Strain.php?ID=70678" target="_blank">link</a></html>
 +
</td>
 +
<td>Amp 50 ug/ml</td>
 +
<td>Plasmid length ok (after digestion with SpeI).</td>
 +
<td>
 +
Contains pAH123 HELPER plasmid carrying IntPhi80, low copy Amp, ori-ts 30°C
 +
</td>
 +
<td>Strains 2010</td>
 +
</tr>
 +
 +
<tr>
 +
<td>
 +
BT340
 +
</td>
 +
<td>
 +
''E. coli'' strain
 +
</td>
 +
<td>
 +
28/06/2010
 +
</td>
 +
<td>
 +
<html><a href="http://cgsc.biology.yale.edu/Strain.php?ID=64645" target="_blank">link</a></html>
 +
</td>
 +
<td>Amp 100 ug/ml and Cm 34 ug/ml</td>
 +
<td>Plasmid relative molecular weight ok (after gel run of the undigested plasmid)</td>
 +
<td>
 +
Contains pCP20 helper with yeast FRT recombinase, Amp, Cm, ori-ts 30°C
 +
</td>
 +
<td>Strains 2010</td>
 +
</tr>
 +
 +
<tr>
 +
<td>
 +
DSM15372
 +
</td>
 +
<td>
 +
''E. coli'' DMS15372 strain
 +
</td>
 +
<td>
 +
08/07/2010
 +
</td>
 +
<td>
 +
<html><a href="http://www.dsmz.de/microorganisms/html/strains/strain.dsm015372.html" target="_blank">link</a></html>
 +
</td>
 +
<td>Amp 100 ug/ml</td>
 +
<td>Length ok (after digestion with EcoRI)</td>
 +
<td>
 +
Harbours the pBHR68 plasmid (contains an IPTG-inducible phaCAB operon)
 +
</td>
 +
<td>Strains 2010</td>
 +
</tr>
 +
</table>
</table>

Latest revision as of 14:30, 27 October 2010
Home The Project Materials & Methods Parts The Team Notebook Biological Safety Pavia Gallery Sponsors

FREEZER MANAGEMENT


For a detailed correspondence between our BioBrick "wiki names" and submitted BioBrick codes take a look here.

Biobrick name Description Creation date Strain Vector Quality control Length Store

<partinfo>BBa_E2050</partinfo>

mOrange

08/06/2010

E. coli DH5-alpha

<partinfo>pSB2K3</partinfo>

Seq ok (MIT); orange pellet when contained in <partinfo>pSB2K3</partinfo>

744 bpBioBricks 2010

<partinfo>BBa_K165018</partinfo>

NLS-ADH1 terminator (Silver Standard 5' and 3')

08/06/2010

E. coli DH5-alpha

<partinfo>J63009</partinfo> (Amp)

Seq partially confirmed (MIT) because it lacks a T at the 3', but the ADH1 terminator (i.e. <partinfo>BBa_K165010</partinfo>) is ok. The correct ApE file is here.

253 bpBioBricks 2010

<partinfo>BBa_K165037</partinfo>

tef2 promoter

08/06/2010

E. coli DH5-alpha

<partinfo>pSB1AK3</partinfo>

Seq ok (MIT).

403 bpBioBricks 2010

<partinfo>BBa_P1004</partinfo>

Chloramphenicol resistence
cassette

08/06/2010

E. coli DH5-alpha

<partinfo>pSB1A1</partinfo>

Seq ok (MIT 2009); length ok.

769 bpBioBricks 2010

<partinfo>BBa_J61001</partinfo>

R6K origin

08/06/2010

E. coli DH5-alpha

<partinfo>pSB1A2</partinfo>

Seq ok (MIT).

406 bpBioBricks 2010

<partinfo>BBa_K081008</partinfo>

RBS-luxI

08/06/2010

E. coli DH5-alpha

<partinfo>pSB1A2</partinfo>

Seq ok (MIT); length ok.

664 bpBioBricks 2010

<partinfo>BBa_K125500</partinfo>

GFP fusion brick (Silver Standard 5', Knight Standard 3')

08/06/2010

E. coli DH5-alpha

<partinfo>pSB1A2</partinfo>

Seq partially confirmed (MIT) for unknown reasons: the alignment is fully correct. The ApE file with the correct prefix and suffix is here. Length ok.

718 bpBioBricks 2010

<partinfo>BBa_K208001</partinfo>
(stab-1)

phasin phaP
(Silver Standard 5' and 3')

24/06/2010

E. coli ???

<partinfo>pSB3K3</partinfo>

Seq ok (BMR); length ok.

579 bpBioBricks 2010

<partinfo>BBa_K208001</partinfo>
(stab-2)

phasin phaP
(Silver Standard 5' and 3')

24/06/2010

E. coli ???

<partinfo>pSB3K3</partinfo>

Seq ok (BMR); length ok.

579 bpBioBricks 2010

<partinfo>BBa_J72007</partinfo>

BamHI methyltransferase encoding CRIM plasmid

01/07/2010

E. coli ???

HC Cm resistance

No plasmid in the clone.
For more informations click here.

BioBricks 2010

<partinfo>BBa_J72008</partinfo>

phi80 integration helper plasmid pInt80-649

01/07/2010

E. coli ???

Plasmid (Amp 50 ug/ml resistance)

Length ok.

6335 bpBioBricks 2010

<partinfo>BBa_J72013</partinfo>

BglII methyltransferase encoding CRIM plasmid

01/07/2010

E. coli ???

HC Cm resistance

No plasmid in the clone.
For more informations click here.

BioBricks 2010

<partinfo>BBa_K105012</partinfo>

10 aa flexible protein domain linker

06/08/2010

E. coli DH5-alpha

<partinfo>pSB1A2</partinfo>

-

30 bp BioBricks 2010

<partinfo>BBa_J13002</partinfo>

p(tetR)+RBS34

16/08/2010

E. coli TOP10

<partinfo>pSB1A2</partinfo>

-

74 bp BioBricks 2010

<partinfo>BBa_I13522</partinfo>

Untagged GFP behind a constitutive promoter

16/08/2010

E. coli TOP10

<partinfo>pSB1A2</partinfo>

-

937 bp BioBricks 2010
pSB1C3_RFP

<partinfo>BBa_J04450</partinfo>
in <partinfo>pSB1C3</partinfo>.

??

E. coli DH5alpha

<partinfo>pSB1C3</partinfo>

1069 bp

2009 Distribution
pSB1C3_ccdB

<partinfo>BBa_P1010</partinfo>
in <partinfo>pSB1C3</partinfo>.

??

E. coli DH5alpha

<partinfo>pSB1C3</partinfo>

675 bp

2009 Distribution
Name Ligation Creation date Strain Vector Quality control Length Description

I0
(it was I0-2)
<partinfo>BBa_k300005</partinfo>

<partinfo>BBa_K125500</partinfo> (E-S)
+
<partinfo>BBa_B0015</partinfo> (E-X)

11/06/2010

E. coli DH5-alpha

<partinfo>pSB1AK3</partinfo>

Seq ok (BMR); length ok.

854 bp GFPs + TT

I1
(it was I1-2)
<partinfo>BBa_k300006</partinfo>

<partinfo>BBa_E2050</partinfo> (E-S)
+
<partinfo>BBa_K165018</partinfo> (E-X)

11/06/2010

E. coli DH5-alpha

<partinfo>J63009</partinfo> (Amp)

Seq ok (BMR), with an additional barcode (25bp) after the stop codon of mOrange; length ok.

1029 bp mOrange + NSL-ADH1 terminator

I2
(it was I2-1)

<partinfo>BBa_P1004</partinfo> (E-S)
+
<partinfo>BBa_B0015</partinfo> (E-X)

11/06/2010

E. coli DH5-alpha

<partinfo>pSB1AK3</partinfo>

Seq ok (BMR); Cm resistance ok; length ok.

906 bp Cm resistance + TT

I3
(it was I3-1)
<partinfo>BBa_k300009</partinfo>

<partinfo>BBa_K081008</partinfo> (E-S)
+
<partinfo>BBa_B0015</partinfo> (E-X)

11/06/2010

E. coli DH5-alpha

<partinfo>pSB1AK3</partinfo>

Seq TWO PLASMIDS IN THIS CLONE!(BMR), two overlapped traces; length ok.

801 bp RBS + luxI + TT

I4
(it was I4-2)

<partinfo>BBa_K165037</partinfo> (S-P)
+
I1 (X-P)

18/06/2010

E. coli DH5-alpha

<partinfo>pSB1AK3</partinfo>

Seq ok (BMR); length ok.

1439 bp pTEF + mOrange + NLS + terminator

I5
(it was I5-1)
<partinfo>BBa_k300008</partinfo>

I2 (E-S)
+
<partinfo>BBa_J61001</partinfo> (E-X)

18/06/2010

E. coli TOP10

<partinfo>pSB1A2</partinfo>

Seq ok (BMR); Cm resistance ok; length ok.

1320 bp Cm resistance + TT + R6K ori

I6
(it was I6-2)

I3 (E-S)
+
<partinfo>BBa_T9002</partinfo> (E-X)

18/06/2010

E. coli DH5-alpha

<partinfo>pSB1A3</partinfo>

Seq LONG PART(BMR), but the ligation worked and the sequenced portions are ok; length ok.

2754 bp RBS + luxI + TT + HSL sensor

I7
(it was I7-3)
<partinfo>BBa_k300024</partinfo>

<partinfo>BBa_J23118</partinfo> (S-P)
+
I6 (X-P)

25/06/2010

E. coli TOP10

<partinfo>BBa_J61002</partinfo>
(Amp)

Length ok.

2795 bp constitutive promoter + RBS + luxI + TT + HSL sensor

I8
(it was I8-5)
<partinfo>BBa_K300021</partinfo>

<partinfo>BBa_J23110</partinfo> (S-P)
+
I6 (X-P)

25/06/2010

E. coli TOP10

<partinfo>BBa_J61002</partinfo>
(Amp)

Length ok.

2795 bp constitutive promoter + RBS + luxI + TT + HSL sensor

I9
(it was I9-1)
<partinfo>BBa_K300022</partinfo>

<partinfo>BBa_J23114</partinfo> (S-P)
+
I6 (X-P)

25/06/2010

E. coli TOP10

<partinfo>BBa_J61002</partinfo>
(Amp)

Length ok.

2795 bp constitutive promoter + RBS + luxI + TT + HSL sensor

I10
(it was I10-1)
<partinfo>BBa_K300023</partinfo>

<partinfo>BBa_J23116</partinfo> (S-P)
+
I6 (X-P)

25/06/2010

E. coli TOP10

<partinfo>BBa_J61002</partinfo>
(Amp)

Length ok.

2795 bp constitutive promoter + RBS + luxI + TT + HSL sensor

I12
(it was I12-2)
<partinfo>BBa_k300019</partinfo>

<partinfo>BBa_J23105</partinfo> (S-P)
+
I6 (X-P)

16/07/2010

E. coli TOP10

<partinfo>BBa_J61002</partinfo>
(Amp)

Seq ok (BMR), length ok.

2795 bp constitutive promoter + RBS + luxI + TT + HSL sensor

I14
(it was I14-1)
<partinfo>BBa_k300030</partinfo>

<partinfo>BBa_J23118</partinfo> (S-P)
+
I3 (X-P)

16/07/2010

E. coli DH5-alpha

<partinfo>BBa_J61002</partinfo>
(Amp)

Seq ok (BMR), length ok.

842 bp constitutive promoter + RBS + luxI + TT

RING

I5 (X-S) on itself:
Cm resistance (<partinfo>BBa_P1004</partinfo>) + TT (<partinfo>BBa_B0015</partinfo>) + R6K ori (<partinfo>BBa_J61001</partinfo>)

16/07/2010

E. coli DH5-alpha

Plasmid (Cm resistance, conditional thermosensitive ORI; works in PIR strains)

Tested here.

1328 bp

Cm resistance + TT + R6K ori

I15
(it was I15-1)
<partinfo>BBa_k300028</partinfo>

<partinfo>BBa_J23110</partinfo> (S-P)
+
I3 (X-P)

23/07/2010

E. coli DH5-alpha

<partinfo>BBa_J61002</partinfo>
(Amp)

Length ok.

842 bp constitutive promoter + RBS + luxI + TT

I16
(it was I16-1)
<partinfo>BBa_k300029</partinfo>

<partinfo>BBa_J23116</partinfo> (S-P)
+
I3 (X-P)

16/07/2010

E. coli DH5-alpha

<partinfo>BBa_J61002</partinfo>
(Amp)

Seq ok (BMR), length ok.

842 bp constitutive promoter + RBS + luxI + TT

I17
(it was I17-1)
<partinfo>BBa_k300025</partinfo>

<partinfo>BBa_J23101</partinfo> (S-P)
+
I3 (X-P)

16/07/2010

E. coli DH5-alpha

<partinfo>BBa_J61002</partinfo>
(Amp)

Seq ok (BMR), length ok.

842 bp constitutive promoter + RBS + luxI + TT

I18
(it was I18-1)
<partinfo>BBa_k300026</partinfo>

<partinfo>BBa_J23105</partinfo> (S-P)
+
I3 (X-P)

16/07/2010

E. coli DH5-alpha

<partinfo>BBa_J61002</partinfo>
(Amp)

Seq ok (BMR), length ok.

842 bp constitutive promoter + RBS + luxI + TT

I19
(it was I19-1)
<partinfo>BBa_k300027</partinfo>

<partinfo>BBa_J23106</partinfo> (S-P)
+
I3 (X-P)

16/07/2010

E. coli DH5-alpha

<partinfo>BBa_J61002</partinfo>
(Amp)

Seq ok (BMR), length ok.

842 bp constitutive promoter + RBS + luxI + TT

I20
(it was I20M-1)
<partinfo>BBa_k300002</partinfo>

Pha10S-1 (X-S) (infos here)
+
<partinfo>pSB1AK3</partinfo> (X-S)

13/08/2010

E. coli DH5-alpha

<partinfo>pSB1AK3</partinfo>

Seq ok (BMR), length ok.

576 bp

Pha10S

I21
(it was I21-4)
<partinfo>BBa_k300003</partinfo>

PhaSS-1 (X-S) (infos here)
+
<partinfo>pSB1A3</partinfo> (X-S)

10/08/2010

E. coli DH5-alpha

<partinfo>pSB1A3</partinfo>

Seq ok (BMR), length ok.

576 bp

PhaSS

I22
(it was I22-1)
<partinfo>BBa_k300031</partinfo>

<partinfo>BBa_K081009</partinfo> (E-S)
+
<partinfo>BBa_B0015</partinfo> (E-X)

10/08/2010

E. coli DH5-alpha

<partinfo>pSB1AK3</partinfo>

Length ok.

825 bp

RBS + lasI + terminator

I23
(it was I23-1)

<partinfo>BBa_R0062</partinfo> (S-P)
+
I3 (X-P)

10/08/2010

E. coli DH5-alpha

<partinfo>pSB1A2</partinfo>

Length ok.

725 bp

plux + RBS + luxI + TT

I24
(it was I24-1)

<partinfo>BBa_R0062</partinfo> (S-P)
+
I22 (X-P)

13/08/2010

E. coli DH5-alpha

<partinfo>pSB1A2</partinfo>

Length ok.

886 bp

plux + RBS + lasI + terminator

I26
(it was I26-2)
<partinfo>BBa_k300081</partinfo>

<partinfo>BBa_K105012</partinfo> (S-P)
+
I21 (X-P)

13/08/2010

E. coli DH5-alpha

<partinfo>pSB1A2</partinfo>

Seq ok (BMR), length ok.

612 bp

linker + PhaSS

I29
(it was I29-1)

<partinfo>BBa_T9002</partinfo> (S-P)
+
I23 (X-P)

31/08/2010

E. coli TOP10

<partinfo>pSB1A3</partinfo>

Length ok.

2678 bp

HLS sensor + plux + RBS + luxI + TT

I30

<partinfo>BBa_T9002</partinfo> (S-P)
+
I24 (X-P)

E. coli TOP10

<partinfo>pSB1A3</partinfo>

2839 bp

HSL sensor + plux + RBS + lasI + terminator

I31
(it was I31-1)
<partinfo>BBa_K300079</partinfo>

I20 (E-S)
+
<partinfo>BBa_K105012</partinfo> (E-X)

23/08/2010

E. coli DH5-alpha

<partinfo>pSB1A2</partinfo>

Seq ok (BMR), length ok.

612 bp

Pha10S + linker

I32
(it was I32-2)

I20 (E-S)
+
I21 (E-X)

23/08/2010

E. coli DH5-alpha

<partinfo>pSB1A3</partinfo>

Seq ok (BMR), length ok.

1158 bp

Pha10S + PhaSS

I33
(it was I33-1)

I20 (E-S)
+
I0 (E-X)

23/08/2010

E. coli DH5-alpha

<partinfo>pSB1AK3</partinfo>

Seq ok (BMR), length ok.

1436 bp

Pha10S + GFPs + TT

I34
(it was I34-1)

I21 (E-S)
+
I0 (E-X)

23/08/2010

E. coli DH5-alpha

<partinfo>pSB1AK3</partinfo>

Seq ok (BMR), length ok.

1436 bp

PhaSS + GFPs + TT

I35
(it was I35-1)
<partinfo>BBa_K300080</partinfo>

I21 (E-S)
+
<partinfo>BBa_K105012</partinfo> (E-X)

23/08/2010

E. coli DH5-alpha

<partinfo>pSB1A2</partinfo>

Seq ok (BMR), length ok.

612 bp

PhaSS + linker

I36
(it was I36-7)

I21 (E-S)
+
I21 (E-X)

24/08/2010

E. coli DH5-alpha

<partinfo>pSB1A3</partinfo>

Length ok.

1158 bp

PhaSS + PhaSS

I37
(it was I37-1)

INTEIN (E-S)
+
I0 (E-X)

23/08/2010

E. coli DH5-alpha

<partinfo>pSB1AK3</partinfo>

Seq ok (BMR), length ok.

1367 bp

INTEIN + GFPs + TT

I38
(it was I38-3)

<partinfo>BBa_F2620</partinfo> (E-S)
+
<partinfo>BBa_B0034</partinfo> (E-X)

23/08/2010

E. coli DH5-alpha

<partinfo>pSB1A2</partinfo>

Seq ok (BMR), length ok.

1081 bp

HSL inducible system

I39
(it was I39-5)

<partinfo>BBa_J13002</partinfo> (S-P)
+
I20 (X-P)

24/08/2010

E. coli DH5-alpha

<partinfo>pSB1A2</partinfo>

Seq ok (BMR), length ok.

656 bp

pTet + Pha10S

I40
(it was I40-1)

I26 (E-S)
+
INTEIN (E-X)

25/08/2010

E. coli DH5-alpha

pMA (HC Amp resistance)

Length ok.

1125 bp

linker + PhaSS + INTEIN

I41
(it was I41-1)

I26 (E-S)
+
I0 (E-X)

23/08/2010

E. coli DH5-alpha

<partinfo>pSB1AK3</partinfo>

Seq ok (BMR), length ok.

1472 bp

linker + PhaSS + GFPs + TT

I42
(it was I42-3)

CREAM (E-P)
+
<partinfo>BBa_K173001</partinfo> (E-P)

23/08/2010

E. coli BW23474

CREAM

Length ok.

3068 bp

CREAM fragment + Cm resistance + TT + R6K ori + constitutive GFP

I43

(it was I43-2)

CREAM (E-P)
+
<partinfo>BBa_J23101</partinfo> (E-P)

23/08/2010

E. coli BW23474

CREAM

Length ok.

3026 bp

CREAM fragment + Cm resistance + TT + R6K ori + constitutive promoter

I44
(it was I44-2)

CREAM (E-P)
+
<partinfo>BBa_I52002</partinfo> (E-P)

15/09/2010

E. coli DB3.1

CREAM

Seq ok (BMR).

3239 bp

CREAM fragment + Cm resistance + TT + R6K ori + marker ccdB

I45
FAILED

CREAM (HindIII) on itself

23/08/2010

E. coli BW23474

CREAM

2149 bp

CREAM fragment + Cm resistance + TT + R6K ori

I47
(it was I47-13)
<partinfo>BBa_K300086</partinfo>

<partinfo>BBa_J13002</partinfo> (S-P) + I33 (X-P)

02/09/2010

E. coli TOP10

<partinfo>pSB1A2</partinfo>

Seq ok (BMR), length ok.

1516 bp

pTet + Pha10S + GFPs + TT

I48
(it was I48-1)
<partinfo>BBa_K300088</partinfo>

I39 (S-P) + I34 (X-P)

31/08/2010

E. coli TOP10

<partinfo>pSB1A2</partinfo>

Seq ok (BMR), length ok.

2098 bp

pTet + Pha10S + PhaSS + GFPs + TT

I49
(it was I49-1)
<partinfo>BBa_K300090</partinfo>

I39 (S-P) + I41 (X-P)

31/08/2010

E. coli TOP10

<partinfo>pSB1A2</partinfo>

Seq ok (BMR), length ok.

2134 bp

pTet + Pha10S + linker + PhaSS + GFPs + TT

I50
FAILED (no trasformants on plate)

I44 (E-H) on itself

28/08/2010

E. coli DB3.1

CREAM

3239 bp

CREAM fragment + Cm resistance + TT + R6K ori + marker ccdB

I51
(it was I51-3)
<partinfo>BBa_I52002</partinfo>

CREAM (E-P)
+
<partinfo>BBa_I52002</partinfo> (E-P)

03/09/2010

E. coli DB3.1

CREAM

3239 bp

CREAM fragment + Cm resistance + TT + R6K ori + marker ccdB

I52
(it was I52-c)

I35 (S-P)
+
I37 (X-P)

17/09/2010

E. coli DH5-alpha

<partinfo>pSB1A2</partinfo>

Length ok.

1985 bp

PhaSS + linker + INTEIN + GFPs + TT

I53
(it was I53-c)

I32 (E-S)
+
I37 (E-X)

17/09/2010

E. coli DH5-alpha

<partinfo>pSB1AK3</partinfo>

Length ok.

2531 bp

Pha10S + PhaSS + INTEIN + GFPs + TT

I54
(it was I54-a)

I36 (E-S)
+
I37 (E-X)

17/09/2010

E. coli DH5-alpha

<partinfo>pSB1AK3</partinfo>

Length ok.

2531 bp

PhaSS + PhaSS + INTEIN + GFPs + TT

I55
(it was I55-1)
<partinfo>BBa_K300082</partinfo>

I38 (E-S)
+
I20 (E-X)

02/10/2010

E. coli DH5-alpha

<partinfo>pSB1AK3</partinfo>

Seq ok (BMR), length ok.

1663 bp

HSL inducible system + Pha10S

I56
(it was I56-a)

I26 (E-S)
+
I41 (E-X)

17/09/2010

E. coli DH5-alpha

<partinfo>pSB1AK3</partinfo>

Length ok.

2090 bp

linker + PhaSS + linker + PhaSS + GFPs + TT

I57
(it was I57-a)

I40 (E-S)
+
I0 (E-X)

17/09/2010

E. coli DH5-alpha

<partinfo>pSB1AK3</partinfo>

Length ok.

1985 bp

linker + PhaSS + INTEIN + GFPs + TT

I58
(it was I58-c)

I38 (E-S)
+
I31 (E-X)

17/09/2010

E. coli DH5-alpha

<partinfo>pSB1A2</partinfo>

Length ok.

1699 bp

HSL inducible system + Pha10S + linker

I59
(it was I59-b)

I21 (E-S)
+
I34 (E-X)

17/09/2010

E. coli DH5-alpha

<partinfo>pSB1AK3</partinfo>

Length ok.

2018 bp

PhaSS + PhaSS + GFPs + TT

I60
(it was I60-a)

I20 (S-P)
+
I26 (X-P)

17/09/2010

E. coli DH5-alpha

<partinfo>pSB1AK3</partinfo>

Seq ok (BMR), length ok.

1194 bp

Pha10S + linker + PhaSS

I61
(it was I61-a)

I31 (E-S)
+
I37 (E-X)

17/09/2010

E. coli DH5-alpha

<partinfo>pSB1AK3</partinfo>

Length ok.

1985 bp

Pha10S + linker + INTEIN + GFPs + TT

I62
(it was I62-E)

I39 (S-P)
+
I59 (X-P)

27/09/2010

E. coli TOP10

<partinfo>pSB1A2</partinfo>

Length ok.

2678 bp

pTet + Pha10S + PhaSS + PhaSS + GFPs + TT

I63
(it was I63-1)
<partinfo>BBa_K300091</partinfo>

I38 (E-S)
+
I61 (E-X)

24/09/2010

E. coli TOP10

<partinfo>pSB1AK3</partinfo>

Length ok.

3072 bp

HSL inducible system + Pha10S + linker + INTEIN + GFPs + TT

I64
(it was I64-2)
<partinfo>BBa_K300092</partinfo>

I58 (E-S)
+
I52 (E-X)

24/09/2010

E. coli TOP10

<partinfo>pSB1A2</partinfo>

Length ok.

3690 bp

HSL inducible system + Pha10S + linker + PhaSS + linker + INTEIN + GFPs + TT

I65
(it was I65-3)
<partinfo>BBa_K300099</partinfo>

I39 (S-P)
+
I56 (X-P)

24/09/2010

E. coli TOP10

<partinfo>pSB1A2</partinfo>

Length ok.

2752 bp

pTet + Pha10S + linker + PhaSS + linker + PhaSS + GFPs + TT

I66
(it was I66-1)

I60 (E-S)
+
I57 (E-X)

24/09/2010

E. coli TOP10

<partinfo>pSB1AK3</partinfo>

Length ok.

3185 bp

Pha10S + linker + PhaSS + linker + PhaSS + INTEIN + GFPs + TT

I70
(it was I70-1)
<partinfo>BBa_K300007</partinfo>

MyYeast3 (E-S)
+
I4 (E-S)

01/10/10

E. coli TOP10

MyYeast (Amp100 in coli)

5187bp

MyYeast vector + I4 in cloning site (Ptef1 + mOrange + Tadh1)

I71
(it was I71-1)

MyYeast3 (E-S)
+
I1 (E-S)

01/10/10

E. coli TOP10

MyYeast (Amp100 in coli)

4778bp

MyYeast vector + I1 in cloning site (mOrange + Tadh1, NO promoter)

I72
(it was I70-x)

<partinfo>BBa_J04450</partinfo>(E-P)+<partinfo>pSB4C5</partinfo>(E-P)

E. coli TOP10

<partinfo>pSB4C5</partinfo>

4290bp

mRFP under pLac promoter in LC plasmid pSB4C5

I73
(it was I73-1)

I38 (E-S)
+
I53 (E-X)

02/10/2010

E. coli TOP10

<partinfo>pSB1AK3</partinfo>

Length ok.

3618 bp

HSL inducible system + Pha10S + PhaSS + INTEIN + GFPs + TT

I74
(it was I74-8)

I31 (E-S)
+
I35 (E-X)

04/10/2010

E. coli TOP10

<partinfo>pSB1A2</partinfo>

Length ok.

1230 bp

Pha10S + linker + PhaSS + linker

I75
(it was I75-x)

I55 (E-S)
+
I37 (E-X)

Not screened yet.

E. coli TOP10

<partinfo>pSB1AK3</partinfo>

Not screened yet.

3036 bp

HSL inducible system + Pha10S + INTEIN + GFPs + TT

I76
(it was I76-x)

I55 (E-S)
+
I54 (E-X)

Not screened yet.

E. coli DH5-alpha

<partinfo>pSB1AK3</partinfo>

Not screened yet.

4191 bp

HSL inducible system + Pha10S + PhaSS + PhaSS + INTEIN + GFPs + TT

I77
(it was I77-x)

I55 (E-S)
+
I57 (E-X)

Not screened yet.

E. coli TOP10

<partinfo>pSB1AK3</partinfo>

Not screened yet.

3654 bp

HSL inducible system + Pha10S + linker + PhaSS + INTEIN + GFPs + TT

I78
(it was I78-3)
<partinfo>BBa_K300084</partinfo>

I74 (E-S)
+
INTEIN_C3 (E-X)

07/10/10

E. coli TOP10

<partinfo>pSB1C3</partinfo>

Seq ok (BMR), length ok.

1743 bp

Pha10S + linker + PhaSS + linker + INTEIN

I79
(it was I79-2)
<partinfo>BBa_K300083</partinfo>

I60 (E-S)
+
INTEIN_C3 (E-X)

07/10/10

E. coli STBL3

<partinfo>pSB1C3</partinfo>

Seq ok (BMR), length ok.

1707 bp

Pha10S + linker + PhaSS + INTEIN

I80
(it was I80-4)
<partinfo>BBa_K300010</partinfo>

I3_1C3 (S-P)
+
<partinfo>BBa_F2620</partinfo> (X-P)

11/10/10

E. coli TOP10

<partinfo>pSB1C3</partinfo>

Seq ok (BMR), length ok.

1707 bp

RBS + luxI + TT + <partinfo>BBa_F2620</partinfo>

INTEIN
<partinfo>BBa_K300004</partinfo>

Intein

13/08/2010

E. coli TOP10

pMA
(HC Amp resistance)

Mr.Gene DNA synthesis

507 bp

INTEIN

INTEIN_C3

Intein

02/10/2010

E. coli TOP10

<partinfo>pSB1C3</partinfo>

Mr.Gene DNA synthesis

507 bp

INTEIN in <partinfo>pSB1C3</partinfo>

YEAST
MR.G
<partinfo>BBa_K300980</partinfo>

YEAST fragment

15/09/2010

E. coli DH5-alpha

pMA
(HC Amp resistance)

Mr.Gene DNA synthesis

1695 bp

YEAST fragment

CRIM
MR.G
<partinfo>BBa_K300983</partinfo>

CREAM fragment

13/08/2010

E. coli TOP10

pMK-RQ
(HC Kan resistance)

Mr.Gene DNA synthesis

829 bp

CREAM fragment

CREAM
(it was MyCRIM)
<partinfo>BBa_K300982</partinfo>

CRIM
MR.G (A-A)
+
I5 (S-X)

21/08/2010

E. coli BW23474

CREAM

Length ok.

2149 bp

CREAM fragment + Cm resistance + TT + R6K ori

I7-4C5
(it was I7-4C5-2)

I7 (E-P)
+
<partinfo>pSB4C5</partinfo>(E-P)

16/07/2010

E. coli TOP10

<partinfo>pSB4C5</partinfo>

Seq ok (BMR), length ok.

2795 bp constitutive promoter + RBS + luxI + TT + HSL sensor in <partinfo>pSB4C5</partinfo>

I8-4C5
(it was I8-4C5-2)

I8 (E-P)
+
<partinfo>pSB4C5</partinfo> (E-P)

16/07/2010

E. coli TOP10

<partinfo>pSB4C5</partinfo>

Seq ok (BMR), length ok.

2795 bp constitutive promoter + RBS + luxI + TT + HSL sensor in <partinfo>pSB4C5</partinfo>

I10-4C5
(it was I10-4C5-1)

I10 (E-P)
+
<partinfo>pSB4C5</partinfo> (E-P)

16/07/2010

E. coli TOP10

<partinfo>pSB4C5</partinfo>

Length ok.

2795 bp

constitutive promoter + RBS + luxI + TT + HSL sensor in <partinfo>pSB4C5</partinfo>

I12-4C5
(it was I12-4C5-1)

I12 (E-P)
+
<partinfo>pSB4C5</partinfo> (E-P)

25/07/2010

E. coli TOP10

<partinfo>pSB4C5</partinfo>

Length ok.

2795 bp

constitutive promoter + RBS + luxI + TT + HSL sensor in <partinfo>pSB4C5</partinfo>

I14-4C5
(it was I14-4C5-2)

I14 (E-P)
+
<partinfo>pSB4C5</partinfo> (E-P)

23/07/2010

E. coli DH5-alpha

<partinfo>pSB4C5</partinfo>

Length ok.

842 bp

constitutive promoter + RBS + luxI + TT + HSL sensor in <partinfo>pSB4C5</partinfo>

I15-4C5
(it was I15-4C5-2)

I15 (E-P)
+
<partinfo>pSB4C5</partinfo> (E-P)

13/08/2010

E. coli TOP10

<partinfo>pSB4C5</partinfo>

Length ok.

842 bp

constitutive promoter + RBS + luxI + TT in <partinfo>pSB4C5</partinfo>

I16-4C5
(it was I16-4C5-1)

I16 (E-P)
+
<partinfo>pSB4C5</partinfo> (E-P)

23/07/2010

E. coli DH5-alpha

<partinfo>pSB4C5</partinfo>

Length ok.

842 bp

constitutive promoter + RBS + luxI + TT in <partinfo>pSB4C5</partinfo>

I17-4C5
(it was I17-4C5-1)

I17 (E-P)
+
<partinfo>pSB4C5</partinfo> (E-P)

25/07/2010

E. coli DH5-alpha

<partinfo>pSB4C5</partinfo>

Length ok.

842 bp

constitutive promoter + RBS + luxI + TT in <partinfo>pSB4C5</partinfo>

I18-4C5
(it was I18-4C5-1)

I18 (E-P)
+
<partinfo>pSB4C5</partinfo> (E-P)

25/07/2010

E. coli DH5-alpha

<partinfo>pSB4C5</partinfo>

Length ok.

842 bp

constitutive promoter + RBS + luxI + TT in <partinfo>pSB4C5</partinfo>

I19-4C5
(it was I19-4C5-1)

I19 (E-P)
+
<partinfo>pSB4C5</partinfo> (E-P)

25/07/2010

E. coli DH5-alpha

<partinfo>pSB4C5</partinfo>

Length ok.

842 bp

constitutive promoter + RBS + luxI + TT in <partinfo>pSB4C5</partinfo>

I27

I23 (E-P)
+
<partinfo>pSB4C5</partinfo> (E-P)

17/08/2010

E. coli TOP10

<partinfo>pSB4C5</partinfo>

725 bp

plux + RBS + luxI + TT in <partinfo>pSB4C5</partinfo>

I28

I24 (E-P)
+
<partinfo>pSB4C5</partinfo> (E-P)

17/08/2010

E. coli TOP10

<partinfo>pSB4C5</partinfo>

886 bp

plux + RBS + lasI + terminator in <partinfo>pSB4C5</partinfo>

BBa_J23100
_4C5
(it was <partinfo>BBa_J23100</partinfo>
_4C5-1)

<partinfo>BBa_J23100</partinfo>
+
<partinfo>BBa_B0034</partinfo>
+
<partinfo>BBa_E1010</partinfo>
+
<partinfo>BBa_B0015</partinfo> in <partinfo>pSB4C5</partinfo>.

27/08/2010

E. coli TOP10

<partinfo>pSB4C5</partinfo>

Seq ok (BMR), length ok.

904 bp

constitutive promoter + RBS + mRFP1 + TT in <partinfo>pSB4C5</partinfo>

BBa_J23101
_4C5
(it was <partinfo>BBa_J23101</partinfo>
_4C5-1)

<partinfo>BBa_J23101</partinfo>
+
<partinfo>BBa_B0034</partinfo>
+
<partinfo>BBa_E1010</partinfo>
+
<partinfo>BBa_B0015</partinfo> in <partinfo>pSB4C5</partinfo>.

27/08/2010

E. coli TOP10

<partinfo>pSB4C5</partinfo>

Seq ok (BMR), length ok.

904 bp

constitutive promoter + RBS + mRFP1 + TT in <partinfo>pSB4C5</partinfo>

BBa_J23105
_4C5
(it was <partinfo>BBa_J23105</partinfo>
_4C5-1)

<partinfo>BBa_J23105</partinfo>
+
<partinfo>BBa_B0034</partinfo>
+
<partinfo>BBa_E1010</partinfo>
+
<partinfo>BBa_B0015</partinfo> in <partinfo>pSB4C5</partinfo>.

27/08/2010

E. coli TOP10

<partinfo>pSB4C5</partinfo>

Length ok.

904 bp

constitutive promoter + RBS + mRFP1 + TT in <partinfo>pSB4C5</partinfo>

BBa_J23106
_4C5
(it was <partinfo>BBa_J23106</partinfo>
_4C5-1)

<partinfo>BBa_J23106</partinfo>
+
<partinfo>BBa_B0034</partinfo>
+
<partinfo>BBa_E1010</partinfo>
+
<partinfo>BBa_B0015</partinfo> in <partinfo>pSB4C5</partinfo>.

27/08/2010

E. coli TOP10

<partinfo>pSB4C5</partinfo>

Length ok.

904 bp

constitutive promoter + RBS + mRFP1 + TT in <partinfo>pSB4C5</partinfo>

BBa_J23110
_4C5
(it was <partinfo>BBa_J23110</partinfo>
_4C5-1)

<partinfo>BBa_J23110</partinfo>
+
<partinfo>BBa_B0034</partinfo>
+
<partinfo>BBa_E1010</partinfo>
+
<partinfo>BBa_B0015</partinfo> in <partinfo>pSB4C5</partinfo>.

27/08/2010

E. coli TOP10

<partinfo>pSB4C5</partinfo>

Length ok.

904 bp

constitutive promoter + RBS + mRFP1 + TT in <partinfo>pSB4C5</partinfo>

BBa_J23114
_4C5
(it was <partinfo>BBa_J23114</partinfo>
_4C5-1)

<partinfo>BBa_J23114</partinfo>
+
<partinfo>BBa_B0034</partinfo>
+
<partinfo>BBa_E1010</partinfo>
+
<partinfo>BBa_B0015</partinfo> in <partinfo>pSB4C5</partinfo>.

27/08/2010

E. coli TOP10

<partinfo>pSB4C5</partinfo>

Length ok.

904 bp

constitutive promoter + RBS + mRFP1 + TT in <partinfo>pSB4C5</partinfo>

BBa_J23116
_4C5
(it was <partinfo>BBa_J23116</partinfo>
_4C5-1)

<partinfo>BBa_J23116</partinfo>
+
<partinfo>BBa_B0034</partinfo>
+
<partinfo>BBa_E1010</partinfo>
+
<partinfo>BBa_B0015</partinfo> in <partinfo>pSB4C5</partinfo>.

30/08/2010

E. coli TOP10

<partinfo>pSB4C5</partinfo>

Length ok.

904 bp

constitutive promoter + RBS + mRFP1 + TT in <partinfo>pSB4C5</partinfo>

BBa_J23118
_4C5
(it was <partinfo>BBa_J23118</partinfo>
_4C5-1)

<partinfo>BBa_J23118</partinfo>
+
<partinfo>BBa_B0034</partinfo>
+
<partinfo>BBa_E1010</partinfo>
+
<partinfo>BBa_B0015</partinfo> in <partinfo>pSB4C5</partinfo>.

30/08/2010

E. coli TOP10

<partinfo>pSB4C5</partinfo>

Seq ok (BMR), length ok.

904 bp

constitutive promoter + RBS + mRFP1 + TT in <partinfo>pSB4C5</partinfo>
Strain name Description Arrival date Infos Resistance Quality control Notes Store

MG1655(Seq)
(MG)

E. coli wild type strain fully sequenced

28/06/2010

link

- Genome fully sequenced Strains 2010

MC1061
(MC)

E. coli strain

28/06/2010

link

- Strain used by JC Anderson et al. for integration Strains 2010

BW25141
(BW1)

E. coli pir+ strain

28/06/2010

link

- Actually replicates the conditional replication origin R6K LC pir Strains 2010

BW25142
(BW2)

E. coli pir116 strain

28/06/2010

link

- Actually replicates the conditional replication origin R6K HC pir Strains 2010

BW23473

E. coli pir+ strain

19/07/2010

link

- LC pir Strains 2010

BW23474

E. coli pir116 strain

28/06/2010

link

- Actually replicates the conditional replication origin R6K HC pir Strains 2010

BW5328/pAH123

E. coli strain

28/06/2010

link

Amp 50 ug/ml Plasmid length ok (after digestion with SpeI).

Contains pAH123 HELPER plasmid carrying IntPhi80, low copy Amp, ori-ts 30°C

Strains 2010

BT340

E. coli strain

28/06/2010

link

Amp 100 ug/ml and Cm 34 ug/ml Plasmid relative molecular weight ok (after gel run of the undigested plasmid)

Contains pCP20 helper with yeast FRT recombinase, Amp, Cm, ori-ts 30°C

Strains 2010

DSM15372

E. coli DMS15372 strain

08/07/2010

link

Amp 100 ug/ml Length ok (after digestion with EcoRI)

Harbours the pBHR68 plasmid (contains an IPTG-inducible phaCAB operon)

Strains 2010
Retrieved from "http://2010.igem.org/Team:UNIPV-Pavia/Freezer"