Team:Harvard/color/parts

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<h2>RNAi constructs</h2>
<h2>RNAi constructs</h2>
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<tr><td><b>Name</b></td><td><b>Description</b></td><td><b>Size</b></td><td><b>Sequence</b></td></tr>
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<tr><td><b>Biobrick Name</b></td><td><b>Description</b></td><td><b>Size</b></td><td><b>Sequence</b></td></tr>
<tr><td>BBa_K382010</td><td>amiRNA construct for lycopene epsilon cyclase (LUT2)knockdown</td><td>413</td><td><a href="https://2010.igem.org/Team:Harvard/c1">link</a></td></tr>
<tr><td>BBa_K382010</td><td>amiRNA construct for lycopene epsilon cyclase (LUT2)knockdown</td><td>413</td><td><a href="https://2010.igem.org/Team:Harvard/c1">link</a></td></tr>
<tr><td>BBa_K382011</td><td>amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown</td><td>413</td><td><a href="https://2010.igem.org/Team:Harvard/c2">link</a></td></tr>
<tr><td>BBa_K382011</td><td>amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown</td><td>413</td><td><a href="https://2010.igem.org/Team:Harvard/c2">link</a></td></tr>
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<h2>Primers Used</h2>
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<h2>Primers</h2>
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<table cellspacing="0">
<tr><td><b>Name</b></td><td><b>Description</b></td><td><b>Sequence</b></td></tr>
<tr><td><b>Name</b></td><td><b>Description</b></td><td><b>Sequence</b></td></tr>

Latest revision as of 00:25, 27 October 2010



parts and primers

RNAi constructs

Biobrick NameDescriptionSizeSequence
BBa_K382010amiRNA construct for lycopene epsilon cyclase (LUT2)knockdown413link
BBa_K382011amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown413link
BBa_K382012amiRNA construct for lycopene beta cyclase (LYC) knockdown413link


Primers

NameDescriptionSequence
CP1LUT2 IgaTAACTTCCTAGTAACCCGCATtctctcttttgtattcc
CP2LUT2 IIgaATGCGGGTTACTAGGAAGTTAtcaaagagaatcaatga
CP3LUT2 IIIgaATACGGGTTACTACGAAGTTTtcacaggtcgtgatatg
CP4LUT2 IVgaAAACTTCGTAGTAACCCGTATtctacatatatattcct
CP13BETA-OHASE IgaTCGTTTAAATCGAAGAGACGCtctctcttttgtattcc
CP14BETA-OHASE IIgaGCGTCTCTTCGATTTAAACGAtcaaagagaatcaatga
CP15BETA-OHASE IIIgaGCATCTCTTCGATATAAACGTtcacaggtcgtgatatg
CP16BETA-OHASE IVgaACGTTTATATCGAAGAGATGCtctacatatatattcct
CP9LYC IgaTAATTATCCAACGACTTGCAGtctctcttttgtattcc
CP10LYC IIgaCTGCAAGTCGTTGGATAATTAtcaaagagaatcaatga
CP11LYC IIIgaCTACAAGTCGTTGCATAATTTtcacaggtcgtgatatg
CP12LYC IVgaAAATTATGCAACGACTTGTAGtctacatatatattcct

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