Team:Yale/Our Project/Protocols/TSI agar

From 2010.igem.org

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<li>12 mg Phenol Red</li>
<li>12 mg Phenol Red</li>
<li>+ Ampicillin</li>
<li>+ Ampicillin</li>
 +
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Latest revision as of 19:35, 24 October 2010

iGEM Yale

protocols

Triple Sugar Iron (TSI) Agar Recipe

  • 500 mL water
  • 1.5 g Meat Extract
  • 1.5 g Yeast Extract
  • 7.5 g Pancreatic Digest of Casein
  • 2.5 g Protease Peptone
  • 0.5 g Dextrose
  • 0.5 g Lactose
  • 0.5 g Sucrose
  • 0.26 g Ferrous Ammonium Sulfate (hexahydrate)
  • 2.5 g NaCl
  • 0.15 g Sodium Thiosulfate
  • 6 g Agar
  • 12 mg Phenol Red
  • + Ampicillin