Team:MIT phage construction
From 2010.igem.org
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<ul> | <ul> | ||
<li><a href="https://2010.igem.org/Team:MIT_toggle">Overview</a></li> | <li><a href="https://2010.igem.org/Team:MIT_toggle">Overview</a></li> | ||
+ | <li><a href="https://2010.igem.org/Team:MIT_tmodel">Modelling</a></li> | ||
<li><a href="https://2010.igem.org/Team:MIT_tconst">Toggle Construction</a></li> | <li><a href="https://2010.igem.org/Team:MIT_tconst">Toggle Construction</a></li> | ||
- | <li><a href=" | + | <li><a href="https://2010.igem.org/Team:MIT_tchara">Characterization</a></li> |
</ul> | </ul> | ||
</dd> | </dd> | ||
+ | </dl> | ||
+ | <dl id ="specialnav"> | ||
<dt><b>Phage</b></dt> | <dt><b>Phage</b></dt> | ||
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</ul> | </ul> | ||
</dd> | </dd> | ||
+ | </dl> | ||
+ | <dl id ="nav"> | ||
<dt><b>Mammalian</b></dt> | <dt><b>Mammalian</b></dt> | ||
<dd> | <dd> | ||
<ul> | <ul> | ||
- | <li><a href=" | + | <li><a href="https://2010.igem.org/Team:MIT_mammalian">Overview</a></li> |
- | <li><a href=" | + | <li><a href="https://2010.igem.org/Team:MIT_mammalian_Standard">New Mammalian Standard </a></li> |
- | <li><a href=" | + | <li><a href="https://2010.igem.org/Team:MIT_mammalian_Circuit">Circuit Design</a></li> |
+ | <li><a href="https://2010.igem.org/Team:MIT_mammalian_Mechanosensation"> Mechanosensation</a></li> | ||
+ | <li><a href="https://2010.igem.org/Team:MIT_mammalian_Bone"> Bone Formation</a></li> | ||
+ | <li><a href="https://2010.igem.org/Team:MIT_mammalian_Switch"> Synthetic Switch</a></li> | ||
</ul> | </ul> | ||
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<b>PARTS</b> | <b>PARTS</b> | ||
<br> | <br> | ||
- | <br><b>BBa_K415100 | + | <br><b><a href="http://partsregistry.org/Part:BBa_K415100">BBa_K415100</a></b> |
+ | <ul><li> | ||
+ | Components: RBS : M13 PVIII</li> | ||
+ | <li> | ||
+ | Explanation: This is a basic translational unit: a ribosome-binding site and the p8 gene. You can attach any promoter in front of it for expression.</li></ul> | ||
<br><img src="http://partsregistry.org/wiki/images/7/7c/K415100.png"> | <br><img src="http://partsregistry.org/wiki/images/7/7c/K415100.png"> | ||
- | <br><br><b>BBa_K415108 | + | |
+ | |||
+ | <br><br><b><a href="http://partsregistry.org/Part:BBa_K415108">BBa_K415108</a></b> | ||
+ | <ul><li> | ||
+ | Components: RBS : M13 PIII</li> | ||
+ | <li> | ||
+ | Explanation: This is a basic translational unit: a ribosome-binding site and the p3 gene. You can attach any promoter in front of it for expression.</li></ul> | ||
<br><img src="http://partsregistry.org/wiki/images/7/7c/K415100.png"> | <br><img src="http://partsregistry.org/wiki/images/7/7c/K415100.png"> | ||
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+ | <br><br><b><a href="http://partsregistry.org/Part:BBa_K415138">BBa_K415138</a></b> | ||
+ | <ul><li> | ||
+ | Components: pLac : RBS : M13 PIII</li> | ||
+ | <li> | ||
+ | Explanation: This is a basic expression unit: a promoter in front of a ribosome-binding site and the p3 gene. We used this to generate constitutive expression of p3 in cells infected with hyperphage in an attempt to rescue the wild-type, non-hairy phenotype. See the results page for an AFM image of this working.</li></ul> | ||
+ | <br><img src="https://static.igem.org/mediawiki/2010/4/4a/138.png"> | ||
+ | <br><br><b><a href="http://partsregistry.org/Part:BBa_K415147">BBa_K415147 - K415152</a></b> | ||
+ | <ul><li> | ||
+ | Components: PluxR/cI : RBS : mCherry : Term : PtetR : RBS : LuxR:Term : Plux/cI : RBS : M13 PVIII-Zipper</li> | ||
+ | <li>Explanation: These parts are K415010 + RBS + P8-fusion protein. As is, this part produces mCherry and the p8-fusion gene in the presence of c6-AHL (and there's also non-trivial leaky expression). When combined with the Hyperphage plasmid, the p8-fusion protein should become incorporated into the phage coat along with normal p8; zippers should then be exposed on the polyphage hairs outside of the cell, allowing for potential cross-linking among cells. With the Collins toggle, UV exposure can be used to control the expression of the p8-fusion protein. Note: below is K415147 only; the rest are similar but with a different translational unit on the end for each linker (Fos, Jun, ACID, BASE, GR1, GR2). | ||
+ | </li></ul> | ||
+ | <br><img src="http://partsregistry.org/wiki/images/a/a3/147.png"> | ||
+ | |||
+ | <div style="text-align:center"> | ||
+ | ← <a href="https://2010.igem.org/Team:MIT_phage_design">Design</a> | ||
+ | | ||
+ | | ||
+ | | ||
+ | <a href="https://2010.igem.org/Team:MIT_phage_results">Results</a> → | ||
+ | </div> | ||
+ | |||
+ | </td> | ||
+ | </table> | ||
</div> | </div> | ||
</div> | </div> | ||
</body> | </body> | ||
</html> | </html> |
Latest revision as of 22:50, 27 October 2010
hairy cells and polymerizing phage - construction |
PARTS BBa_K415100
![]() BBa_K415108
![]() BBa_K415138
![]() BBa_K415147 - K415152
![]() |