Team:UNIPV-Pavia/Material Methods/Measurements/Tecan/test30settembre

From 2010.igem.org

(Difference between revisions)

Latest revision as of 21:28, 25 October 2010

SEPTEMBER, 30TH

PLATE

EXPERIMENT DESCRIPTION

Motivation

The experiment was performed to evaluate the initiation transcription point (in term of absorbance) for self-inducible devices.

Methods

The experiment was performed in M9 according to Protocol 1 on the following measurement parts:

<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300024</partinfo> (wiki name: I7)
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300021</partinfo> (wiki name: I8)
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300022</partinfo> (wiki name: I9)
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300023</partinfo> (wiki name: I10)
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300019</partinfo> (wiki name: I12)
<partinfo>pSB1A2</partinfo> - <partinfo>K173001</partinfo> (positive control)
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_B0032</partinfo> (negative control)
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300030</partinfo> (wiki name I14) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300028</partinfo> (wiki name I15) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300029</partinfo> (wiki name I16) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300025</partinfo> (wiki name I17) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300026</partinfo> (wiki name I18) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300027</partinfo> (wiki name I19) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
<partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo> (negative control)
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300024</partinfo> (wiki name: I7-4C5)
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300021</partinfo> (wiki name: I8-4C5)
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300023</partinfo> (wiki name: I10-4C5)
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300019</partinfo> (wiki name: I12-4C5)

RESULTS

Data were analyzed following the data analysis procedure to evaluate the ODstart. The plots correpsoning to the parts tested are reported:

The ODstart are reported in the table below:

Part	ODstart
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300024</partinfo>	0.0308
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300021</partinfo>	0.1484
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300022</partinfo>	0.4214
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300023</partinfo>	0.3491
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300019</partinfo>	0.3420
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300030</partinfo>	0.0584
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300028</partinfo>	0.1452
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300029</partinfo>	-
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300025</partinfo>	0.1177
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300026</partinfo>	0.5602
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300027</partinfo>	0.3985
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300024</partinfo>	0.2414
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300021</partinfo>	-
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300023</partinfo>	-
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300019</partinfo>	-

Tecan Test

@@ Line 29: / Line 29: @@
 * <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300022</partinfo> (wiki name: I9)
 * <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300023</partinfo> (wiki name: I10)
-* <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300012</partinfo> (wiki name: I12)
+* <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300019</partinfo> (wiki name: I12)
 * <partinfo>pSB1A2</partinfo> - <partinfo>K173001</partinfo> (positive control)
 * <partinfo>pSB1A2</partinfo> - <partinfo>BBa_B0032</partinfo> (negative control)
@@ Line 42: / Line 42: @@
 * <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300021</partinfo> (wiki name: I8-4C5)
 * <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300023</partinfo> (wiki name: I10-4C5)
-* <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300012</partinfo> (wiki name: I12-4C5)
+* <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300019</partinfo> (wiki name: I12-4C5)
 ==RESULTS==
@@ Line 51: / Line 51: @@
 [[Image:UNIPV_Pavia_FluoVSODCo3009.png| 620px | center ]]
+[[Image:UNIPV_Pavia_FluoVSODLC03009.png| 620px | center ]]
 The ODstart are reported in the table below:
@@ Line 65: / Line 66: @@
 || <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300023</partinfo> || 0.3491
 |-
-|| <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300012</partinfo> || 0.3420
+|| <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300019</partinfo> || 0.3420
 |-
 || <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300030</partinfo> || 0.0584
@@ Line 78: / Line 79: @@
 |-
 || <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300027</partinfo> || 0.3985
+|-
+|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300024</partinfo> || 0.2414
+|-
+|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300021</partinfo> || -
+|-
+|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300023</partinfo> || -
+|-
+|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300019</partinfo> || -
 |}