From 2010.igem.org
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| | * <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300022</partinfo> (wiki name: I9) | | * <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300022</partinfo> (wiki name: I9) |
| | * <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300023</partinfo> (wiki name: I10) | | * <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300023</partinfo> (wiki name: I10) |
| - | * <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300012</partinfo> (wiki name: I12) | + | * <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300019</partinfo> (wiki name: I12) |
| | * <partinfo>pSB1A2</partinfo> - <partinfo>K173001</partinfo> (positive control) | | * <partinfo>pSB1A2</partinfo> - <partinfo>K173001</partinfo> (positive control) |
| | * <partinfo>pSB1A2</partinfo> - <partinfo>BBa_B0032</partinfo> (negative control) | | * <partinfo>pSB1A2</partinfo> - <partinfo>BBa_B0032</partinfo> (negative control) |
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| | || <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300023</partinfo> || - | | || <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300023</partinfo> || - |
| | |- | | |- |
| - | || <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300012</partinfo> || 0.5068 | + | || <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300019</partinfo> || 0.5068 |
| | |- | | |- |
| | || <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300030</partinfo> || 0.1609 | | || <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300030</partinfo> || 0.1609 |
Latest revision as of 21:08, 25 October 2010
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AUGUST, 17TH
PLATE
EXPERIMENT DESCRIPTION
Motivation
The experiment was performed to evaluate the initiation transcription point (in term of absorbance) for self-inducible devices.
Methods
The experiment was performed in LB according to Protocol 1 on the following measurement parts:
- <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300024</partinfo> (wiki name: I7)
- <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300021</partinfo> (wiki name: I8)
- <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300022</partinfo> (wiki name: I9)
- <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300023</partinfo> (wiki name: I10)
- <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300019</partinfo> (wiki name: I12)
- <partinfo>pSB1A2</partinfo> - <partinfo>K173001</partinfo> (positive control)
- <partinfo>pSB1A2</partinfo> - <partinfo>BBa_B0032</partinfo> (negative control)
- <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300030</partinfo> (wiki name I14-4C5) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
- <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300029</partinfo> (wiki name I16-4C5) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
- <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300025</partinfo> (wiki name I17-4C5) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
- <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300026</partinfo> (wiki name I18-4C5) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
- <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300027</partinfo> (wiki name I19-4C5) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
- <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo> (negative control)
RESULTS
Data were analyzed following the data analysis procedure to evaluate the ODstart. The plots correpsoning to the parts tested are reported:
The ODstart are reported in the table below:
| Part | ODstart
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| <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300024</partinfo> | 0.1456
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| <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300021</partinfo> | 0
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| <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300022</partinfo> | 0.3257
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| <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300023</partinfo> | -
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| <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300019</partinfo> | 0.5068
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| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300030</partinfo> | 0.1609
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| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300029</partinfo> | 0.3098
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| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300025</partinfo> | 0.1463
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| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300026</partinfo> | -
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| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300027</partinfo> | -
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