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Team:UPO-Sevilla/Notebook/08 06
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<h2>Production Team</h2> | <h2>Production Team</h2> | ||
| - | <p | + | <p>Minipreps were prepared from inocula. We digested with EcoRI and PstI for 2 hours at 37ºC. We ran an electrophoresis of the digestion products and we observed 1'5 kb fragments. We've got fecI-fecR. Colony PCR seems not to be a reliable proof.</p> |
<h2>Assembly Team</h2> | <h2>Assembly Team</h2> | ||
| - | <p>We used 3A method to build: 1+2+7+2, 11+2+13+3, 12+2+13+3, 11+2+16+3 and 12+2+16+3. The necessary steps | + | <p>We used 3A method to build: 1+2+7+2, 11+2+13+3, 12+2+13+3, 11+2+16+3 and 12+2+16+3. The necessary steps were: digestion with accurate digestion enzyme, ligation and transformation. Plates were incubated at 37º over night.</p> |
| - | <p>Minpreps using inocula we | + | <p>Minpreps using inocula we set up the day before. Analytic digestion confirmed the next biobriks: UPO6, UPO7, UPO2+6, UPO12+2 and UPO11+2.</p> |
<a class="return_button" href="/Team:UPO-Sevilla/Notebook/08_05" title="Go to 5th of August"><span>Go to 5th of August</span></a> | <a class="return_button" href="/Team:UPO-Sevilla/Notebook/08_05" title="Go to 5th of August"><span>Go to 5th of August</span></a> | ||
Latest revision as of 14:50, 27 October 2010
August, 6th
Production Team
Minipreps were prepared from inocula. We digested with EcoRI and PstI for 2 hours at 37ºC. We ran an electrophoresis of the digestion products and we observed 1'5 kb fragments. We've got fecI-fecR. Colony PCR seems not to be a reliable proof.
Assembly Team
We used 3A method to build: 1+2+7+2, 11+2+13+3, 12+2+13+3, 11+2+16+3 and 12+2+16+3. The necessary steps were: digestion with accurate digestion enzyme, ligation and transformation. Plates were incubated at 37º over night.
Minpreps using inocula we set up the day before. Analytic digestion confirmed the next biobriks: UPO6, UPO7, UPO2+6, UPO12+2 and UPO11+2.
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