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Team:UPO-Sevilla/Notebook/07 29
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<p><strong>Paola Gallardo.</strong> Transformation of the ligation products in <i>E. coli</i> DH5-α and waited one night to see the results.</p> | <p><strong>Paola Gallardo.</strong> Transformation of the ligation products in <i>E. coli</i> DH5-α and waited one night to see the results.</p> | ||
| - | <p><strong>David Caballero.</strong> | + | <p><strong>David Caballero.</strong> In order to verify that our original sample of <i>fecA*</i>, amplified by PCR using <i>E. coli</i> genome like template, had not problem we analyzed it by 0.8% agarose gel electrophoresis: first <i>fecA*</i> PCR product and two digestion products of this one. Also we did colony PCR and electrophoresis analysis to some white colonies we found in the first plate in which we tried to transform <i>E. coli</i> with <i>fecA*</i>. The spot pattern showed that <i>fecA*</i> PCR product and its digestions were perfect. Moreover we had two candidate from colony PCR analysis. We set up inocula from them and isolated in plate to verify the next day.</p> |
<h2>Assembly Team</h2> | <h2>Assembly Team</h2> | ||
Latest revision as of 22:02, 26 October 2010
July, 29th
Production Team
Paola Gallardo. Transformation of the ligation products in E. coli DH5-α and waited one night to see the results.
David Caballero. In order to verify that our original sample of fecA*, amplified by PCR using E. coli genome like template, had not problem we analyzed it by 0.8% agarose gel electrophoresis: first fecA* PCR product and two digestion products of this one. Also we did colony PCR and electrophoresis analysis to some white colonies we found in the first plate in which we tried to transform E. coli with fecA*. The spot pattern showed that fecA* PCR product and its digestions were perfect. Moreover we had two candidate from colony PCR analysis. We set up inocula from them and isolated in plate to verify the next day.
Assembly Team
We make miniprep of some plasmids and vectors. Digestion and ligation of 1+19 and 18+3. Transformation of 1+19, 2+12, 12+19, 13+3.
DryLab Team
Modeling: Attending to Continuous Systems and Simbilogy (MatLab) seminar set by Luis Merino.
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