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Team:Brown/Notebook/September20
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(New page: ===Results of transformations=== *AraC-J13002 ligation --> 4 to 5 colonies *LacI-J13002 ligation --> 0 transformants Will start overnight culture later today for AraC-J13002 ligation tran...) |
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| + | ==Monday, September 20, 2010== | ||
===Results of transformations=== | ===Results of transformations=== | ||
*AraC-J13002 ligation --> 4 to 5 colonies | *AraC-J13002 ligation --> 4 to 5 colonies | ||
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J13002-LacI Ligation: remainder of DNA added for second attempt transformation of XL1B. | J13002-LacI Ligation: remainder of DNA added for second attempt transformation of XL1B. | ||
| + | |||
| + | ===Overnight Culture=== | ||
| + | Started overnight culture for Arac-J13002 ligation. | ||
| + | (3 μl Amp in 3 mL LB) | ||
| + | picked 3 colonies. | ||
| + | |||
| + | 11:05 PM - placed in 37°C. | ||
Latest revision as of 23:11, 23 October 2010
Monday, September 20, 2010
Results of transformations
- AraC-J13002 ligation --> 4 to 5 colonies
- LacI-J13002 ligation --> 0 transformants
Will start overnight culture later today for AraC-J13002 ligation transformants.
J13002-LacI Ligation: remainder of DNA added for second attempt transformation of XL1B.
Overnight Culture
Started overnight culture for Arac-J13002 ligation. (3 μl Amp in 3 mL LB) picked 3 colonies.
11:05 PM - placed in 37°C.
