Week of 8/2

From 2010.igem.org

(Difference between revisions)
(New page: ==August 2nd== *Miniprep - R0061 - R1062 - J23100 *Digests - K091146 (SP) - R1062 (SP) - J23100 (SP) - K091107 (SP) - Lux+RBS+A3 (XP+phosphatase) - RFP+RBS (XP+phophatase) ...)
 
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Line 25: Line 25:
  - K145150
  - K145150
  - Ladder
  - Ladder
 +
<center>[[Image:8-2-10.jpg]]</center>
*Ligations
*Ligations
Line 31: Line 32:
  - K091146 + RFP+RBS
  - K091146 + RFP+RBS
  - K091107 + RFP+RBS
  - K091107 + RFP+RBS
-
 
-
 
==August 3rd==
==August 3rd==
Line 63: Line 62:
  - K091146  
  - K091146  
  - Ladder
  - Ladder
 +
<center>[[Image:8-3-10.jpg]]</center>
The last two should have been about 7000 base pairs but it was much lower so the pieces were not cut out. The first piece (K091146) was cut out.
The last two should have been about 7000 base pairs but it was much lower so the pieces were not cut out. The first piece (K091146) was cut out.
Line 84: Line 84:
   - 3C
   - 3C
   - 3D
   - 3D
 +
<center>[[Image:8-3-10 001 8-3-10 002.jpg]]</center>
*PCRs did not show up. But, the bands for J24679+B1002 (B) and (D) were extracted
*PCRs did not show up. But, the bands for J24679+B1002 (B) and (D) were extracted
Line 111: Line 112:
  - K091146+RFP+RBS (A-D)
  - K091146+RFP+RBS (A-D)
-
 
+
<center>[[Image:8-4-10.jpg]]</center>
==August 5th==
==August 5th==
Line 151: Line 152:
  - RFP (EP)
  - RFP (EP)
  - RFP (XS)
  - RFP (XS)
-
 
+
<center>[[Image:8-5-10.jpg]]</center>
==August 6th==
==August 6th==
Line 184: Line 185:
  - K091146+RFP (A-C)
  - K091146+RFP (A-C)
  - R1062+RFP (A-C)
  - R1062+RFP (A-C)
 +
<center>[[Image:8-6-10.jpg]]</center>
*Today, we compared our enzymes to Mike Speer's (grad student) enzymes because we aren't sure if they are working correctly.
*Today, we compared our enzymes to Mike Speer's (grad student) enzymes because we aren't sure if they are working correctly.
Line 190: Line 192:
  1)K091107+RFP (a)
  1)K091107+RFP (a)
  2)R0061+RFP (a)
  2)R0061+RFP (a)
 +
 +
 +
 +
[https://2010.igem.org/Team:Penn_State/Notebook Return to Notebook]

Latest revision as of 18:08, 27 October 2010

Contents

August 2nd

  • Miniprep 
- R0061
- R1062
- J23100


  • Digests 
- K091146 (SP)
- R1062 (SP)
- J23100 (SP)
- K091107 (SP)
- Lux+RBS+A3 (XP+phosphatase)
- RFP+RBS (XP+phophatase)


  • Gel order: 
- J23100
- R1062
- K091146
- R0063
- K091107
- K145150
- Ladder
8-2-10.jpg
  • Ligations 
- J23100 + Lux+RBS+A3
- R1062 + RFP+RBS
- K091146 + RFP+RBS
- K091107 + RFP+RBS

August 3rd

  • Colony PCR 
1)K091107+RFP+RBS A-C
2)R1062+RFP+RBS A-D
3)J23100+LuxR+RBS+A3 A-D


  • Digest 
- R0063 (SP)
- K145150 (SP)
- K091146 (SP)
- RFP+RBS (XP+phosphatase)
- Violet pigment 1 (EP)
- Violet pigment 2 (EP)
- A3 (EP+phosphatase)
  • Digest for 6 hours: 
- J24679+B1002 (B)
- J24679+B1002 (D)


  • Gel order: 
- Violet 1
- Violet 2
- K145150 
- R0063
- K091146 
- Ladder
8-3-10.jpg

The last two should have been about 7000 base pairs but it was much lower so the pieces were not cut out. The first piece (K091146) was cut out.

  • Gel number # 2: 
-Row 1
  - Ladder
  - J24679+B1002 (B)
  - J24679+B1002 (D)
Row 2
  - Ladder
  - 1A 
  - 1B
  - 1C
  - 2A
  - 2B
  - 2C
  - 2D
  - 3A
  - 3B
  - 3C
  - 3D
8-3-10 001 8-3-10 002.jpg
  • PCRs did not show up. But, the bands for J24679+B1002 (B) and (D) were extracted


August 4th

  • Miniprep: 
- R0061
  • Digests 
- R0061 (SP+phosphatase)
- R0062 (SP+phosphatase)
- R0063 (SP+phosphatase)
- K145150 (SP+phosphatase)
- K091146 (SP+phosphatase)
- RFP+RBS (XP)
- J23100 (SP+phosphatase)
- Lux+RBS (XP)
  • Grow Up for tomorrow: 
- RFP+RBS
- K091107
- K091146
  • Sent to sequencing: 
- K091146+RFP+RBS (A-D)
8-4-10.jpg

August 5th

  • Digests 
- RFP (E)
- RFP (X)
- RFP (S)
- RFP (P)
- RFP (EP)
- RFP (XS)
- Lux promoters with RFP but used violet pigment as the backbone
  • Minipreps: 
- K091146+RFP+RBS
- RFP+RBS
- K091107
- K091146
  • Ligations: 
- R0061 + RFP+RBS
- R0063 + RFP+RBS
- K091146 + RFP+RBS
- K145150 + RFP+RBS
- K091107 + RFP+RBS
- R1062 + RFP+RBS
  • Colony PCR 
- J23100+Lux+RBS (A-F)
  • Gel order: 
- Ladder
- J23100+Lux+RBS A-F
- Ladder 2
- RFP (E)
- RFP (X)
- RFP (S)
- RFP (P)
- RFP (EP)
- RFP (XS)
8-5-10.jpg

August 6th

  • Miniprep 
-RFP+RBS
  • Gel order of PCR products: 
- Ladder        - Ladder 2
- 1A            - 4B
- 1B            - 4C
- 1C            - 4D 
- 1D            - 5A
- 2A            - 5B
- 2B            - 5C
- 2C            - 6A
- 2D            - 6B
- 3A            - 6C
- 3B 
- 3C
- 3D
- 4A


  • Colony PCR 
- R0063+RFP (A-D)
- K091107+RFP (A-D)
- K145150+RFP (A-D)
- R0061+RFP (A-D)
- K091146+RFP (A-C)
- R1062+RFP (A-C)
8-6-10.jpg
  • Today, we compared our enzymes to Mike Speer's (grad student) enzymes because we aren't sure if they are working correctly.
  • Sent to sequencing: 
1)K091107+RFP (a)
2)R0061+RFP (a)


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