Team:UNIPV-Pavia/Material Methods/Measurements/Tecan/test17agosto

(Difference between revisions)

Latest revision as of 21:08, 25 October 2010

AUGUST, 17TH

The experiment was performed to evaluate the initiation transcription point (in term of absorbance) for self-inducible devices.

The experiment was performed in LB according to Protocol 1 on the following measurement parts:

<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300024</partinfo> (wiki name: I7)
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300021</partinfo> (wiki name: I8)
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300022</partinfo> (wiki name: I9)
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300023</partinfo> (wiki name: I10)
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300019</partinfo> (wiki name: I12)
<partinfo>pSB1A2</partinfo> - <partinfo>K173001</partinfo> (positive control)
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_B0032</partinfo> (negative control)
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300030</partinfo> (wiki name I14-4C5) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300029</partinfo> (wiki name I16-4C5) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300025</partinfo> (wiki name I17-4C5) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300026</partinfo> (wiki name I18-4C5) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300027</partinfo> (wiki name I19-4C5) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
<partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo> (negative control)

Data were analyzed following the data analysis procedure to evaluate the ODstart. The plots correpsoning to the parts tested are reported:

The ODstart are reported in the table below:

Part	ODstart
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300024</partinfo>	0.1456
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300021</partinfo>	0
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300022</partinfo>	0.3257
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300023</partinfo>	-
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300019</partinfo>	0.5068
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300030</partinfo>	0.1609
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300029</partinfo>	0.3098
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300025</partinfo>	0.1463
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300026</partinfo>	-
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300027</partinfo>	-

@@ Line 28: / Line 28: @@
 * <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300022</partinfo> (wiki name: I9)
 * <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300023</partinfo> (wiki name: I10)
-* <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300012</partinfo> (wiki name: I12)
+* <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300019</partinfo> (wiki name: I12)
 * <partinfo>pSB1A2</partinfo> - <partinfo>K173001</partinfo> (positive control)
 * <partinfo>pSB1A2</partinfo> - <partinfo>BBa_B0032</partinfo> (negative control)
@@ Line 58: / Line 58: @@
 || <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300023</partinfo> || -
 |-
-|| <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300012</partinfo> || 0.5068
+|| <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300019</partinfo> || 0.5068
 |-
 || <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300030</partinfo> || 0.1609
@@ Line 75: / Line 75: @@
 <table border="0" width="100%" class="menu">
 <tr>
-<td align="left" width="50%">[[Team:UNIPV-Pavia/Material Methods/Measurements/Tecan/test10agosto|Previous Test]]</td>
+<td align="left" width="50%"></td>
 <td align="right" width="50%">[[Team:UNIPV-Pavia/Material Methods/Measurements/Tecan/test18agosto| Next Test]]</td>
 </tr>