Team:UNIPV-Pavia/Material Methods/Measurements/Tecan/test17agosto

From 2010.igem.org

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* <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300022</partinfo> (wiki name: I9)
* <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300022</partinfo> (wiki name: I9)
* <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300023</partinfo> (wiki name: I10)
* <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300023</partinfo> (wiki name: I10)
-
* <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300012</partinfo> (wiki name: I12)
+
* <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300019</partinfo> (wiki name: I12)
* <partinfo>pSB1A2</partinfo> - <partinfo>K173001</partinfo> (positive control)
* <partinfo>pSB1A2</partinfo> - <partinfo>K173001</partinfo> (positive control)
* <partinfo>pSB1A2</partinfo> - <partinfo>BBa_B0032</partinfo> (negative control)
* <partinfo>pSB1A2</partinfo> - <partinfo>BBa_B0032</partinfo> (negative control)
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|| Part || ODstart
|| Part || ODstart
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|-
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|| <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300024</partinfo> ||  
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|| <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300024</partinfo> || 0.1456
|-
|-
-
|| <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300021</partinfo> ||  
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|| <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300021</partinfo> || 0
|-
|-
-
|| <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300022</partinfo> ||  
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|| <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300022</partinfo> || 0.3257
|-
|-
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|| <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300023</partinfo> ||  
+
|| <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300023</partinfo> || -
|-
|-
-
|| <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300012</partinfo> ||  
+
|| <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300019</partinfo> || 0.5068
|-
|-
-
|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300030</partinfo> ||  
+
|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300030</partinfo> || 0.1609
|-
|-
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|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300028</partinfo> ||  
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|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300029</partinfo> || 0.3098
|-
|-
-
|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300029</partinfo> ||  
+
|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300025</partinfo> || 0.1463
|-
|-
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|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300025</partinfo> ||  
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|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300026</partinfo> || -
|-
|-
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|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300026</partinfo> ||
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|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300027</partinfo> || -
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|-
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|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300027</partinfo> ||
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<table border="0" width="100%" class="menu">
<table border="0" width="100%" class="menu">
<tr>
<tr>
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<td align="left" width="50%">[[Team:UNIPV-Pavia/Material Methods/Measurements/Tecan/test10agosto|Previous Test]]</td>
+
<td align="left" width="50%"></td>
<td align="right" width="50%">[[Team:UNIPV-Pavia/Material Methods/Measurements/Tecan/test18agosto| Next Test]]</td>
<td align="right" width="50%">[[Team:UNIPV-Pavia/Material Methods/Measurements/Tecan/test18agosto| Next Test]]</td>
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Latest revision as of 21:08, 25 October 2010
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AUGUST, 17TH


PLATE


UNIPV Pavia piastra17agosto.png


EXPERIMENT DESCRIPTION

Motivation

The experiment was performed to evaluate the initiation transcription point (in term of absorbance) for self-inducible devices.

Methods

The experiment was performed in LB according to Protocol 1 on the following measurement parts:

  • <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300024</partinfo> (wiki name: I7)
  • <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300021</partinfo> (wiki name: I8)
  • <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300022</partinfo> (wiki name: I9)
  • <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300023</partinfo> (wiki name: I10)
  • <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300019</partinfo> (wiki name: I12)
  • <partinfo>pSB1A2</partinfo> - <partinfo>K173001</partinfo> (positive control)
  • <partinfo>pSB1A2</partinfo> - <partinfo>BBa_B0032</partinfo> (negative control)
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300030</partinfo> (wiki name I14-4C5) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300029</partinfo> (wiki name I16-4C5) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300025</partinfo> (wiki name I17-4C5) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300026</partinfo> (wiki name I18-4C5) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300027</partinfo> (wiki name I19-4C5) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
  • <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo> (negative control)

RESULTS

Data were analyzed following the data analysis procedure to evaluate the ODstart. The plots correpsoning to the parts tested are reported:

UNIPV Pavia FluoVSODHC1708.png
UNIPV Pavia FluoVSODCo1708.png

The ODstart are reported in the table below:

Part ODstart
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300024</partinfo> 0.1456
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300021</partinfo> 0
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300022</partinfo> 0.3257
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300023</partinfo> -
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300019</partinfo> 0.5068
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300030</partinfo> 0.1609
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300029</partinfo> 0.3098
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300025</partinfo> 0.1463
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300026</partinfo> -
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300027</partinfo> -



Next Test

Tecan Test

Retrieved from "http://2010.igem.org/Team:UNIPV-Pavia/Material_Methods/Measurements/Tecan/test17agosto"