Team:St Andrews/project/laboratory/protocols

From 2010.igem.org

(Difference between revisions)

Revision as of 15:11, 23 October 2010 (view source) JWTaylor (Talk | contribs) (→Annealing of Complementary Primers) ← Older edit		Latest revision as of 20:12, 23 October 2010 (view source) JWTaylor (Talk | contribs) (→DNA Extraction)
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	===Transformation===		===Transformation===
-	NEB Turbo Competent E. coli	+	[http://www.neb.com/nebecomm/products/protocolProductC2984.asp NEB Turbo Competent E. coli]
-	Clontech Stellar Competent E. coli.	+	[http://www.clontech.com/products/detail.asp?product_id=215795&tabno=3 Clontech Stellar Competent E. coli.]
	===Restriction Digest===		===Restriction Digest===
Line 32:		Line 32:
	===DNA Extraction===		===DNA Extraction===
-	Quiagen Miniprep	+	[http://www.qiagen.com/products/plasmid/qiaprepminiprepsystem/qiaprepspinminiprepkit.aspx#Tabs=t2 Quiagen Miniprep]
		+
	Isopropanol Extraction of small DNA fragments		Isopropanol Extraction of small DNA fragments
Line 38:		Line 39:
-	Gingko Bioworks - [http://ginkgobioworks.com/support/ Three Antibiotic Assembly (NEB Biobrick Assembly Kit)]	+	Gingko Bioworks - [http://ginkgobioworks.com/support/ 3A Biobrick Assembly] (NEB Biobrick Assembly Kit)
-	OpenWetWare [http://openwetware.org/wiki/In-fusion_biobrick_assembly In Fusion Biobrick Assembly (Primer design and assembly strategy)]	+	OpenWetWare [http://openwetware.org/wiki/In-fusion_biobrick_assembly In Fusion Biobrick Assembly] (Primer design and assembly strategy)
-	Clontech [http://www.clontech.com/products/detail.asp?catalog_id=639621 In Fusion Cloning (In Fusion reaction itself)]	+	Clontech [http://www.clontech.com/products/detail.asp?catalog_id=639621 In Fusion Cloning] (In Fusion reaction itself)
	OpenWetWare [http://openwetware.org/wiki/Endy:Annealing_complementary_primers Endy - Annealing Complementary Primers]		OpenWetWare [http://openwetware.org/wiki/Endy:Annealing_complementary_primers Endy - Annealing Complementary Primers]

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Latest revision as of 20:12, 23 October 2010

University of St Andrews iGEM 2010

Welcome!

University of St Andrews iGEM 2010

Our first year at iGEM!

Protocols

We used a number of protocols from OpenWetWare in addition to the manufacturer's recommended protocols for our materials.

Cell Culture

OpenWetWare [http://openwetware.org/wiki/Bacterial_cell_culture Bacterial Cell Culture]

Transformation

[http://www.neb.com/nebecomm/products/protocolProductC2984.asp NEB Turbo Competent E. coli]

[http://www.clontech.com/products/detail.asp?product_id=215795&tabno=3 Clontech Stellar Competent E. coli.]

Restriction Digest

Promega Enzymes

NEB Biobrick Assembly Kit (Gingko Bioworks)

Ligation

NEB Biobrick Assembly Kit (Gingko Bioworks)

PCR

OpenWetWare [http://openwetware.org/wiki/Knight:Colony_PCR Knight - Colony PCR]

NEB Taq

Finnzymes Phusion

DNA Extraction

[http://www.qiagen.com/products/plasmid/qiaprepminiprepsystem/qiaprepspinminiprepkit.aspx#Tabs=t2 Quiagen Miniprep]

Isopropanol Extraction of small DNA fragments

Construction of BioBricks

Gingko Bioworks - [http://ginkgobioworks.com/support/ 3A Biobrick Assembly] (NEB Biobrick Assembly Kit)

OpenWetWare [http://openwetware.org/wiki/In-fusion_biobrick_assembly In Fusion Biobrick Assembly] (Primer design and assembly strategy)

Clontech [http://www.clontech.com/products/detail.asp?catalog_id=639621 In Fusion Cloning] (In Fusion reaction itself)

OpenWetWare [http://openwetware.org/wiki/Endy:Annealing_complementary_primers Endy - Annealing Complementary Primers]