Team:UNIPV-Pavia/Freezer
From 2010.igem.org
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Line 1: | Line 1: | ||
- | <table width="100%" border="0" > | + | <table width="100%" border="0"> |
<tr> | <tr> | ||
<td colspan="2">{{UNIPV-Pavia/header}}</td> | <td colspan="2">{{UNIPV-Pavia/header}}</td> | ||
</tr> | </tr> | ||
- | <tr><td | + | <tr> |
- | + | <td colspan="2">{{UNIPV-Pavia/menuAlto}}</td> | |
+ | </tr> | ||
+ | <tr><td valign=top> | ||
<!-- Contenuti --> | <!-- Contenuti --> | ||
{{UNIPV-Pavia/Style}} | {{UNIPV-Pavia/Style}} | ||
- | + | <table border="0" align="center" width="100%"><tr><td align="justify" valign="top" style="padding:20px"> | |
- | <html><p align="center"><font size="4"><b> | + | <html><p align="center"><font size="4"><b>FREEZER MANAGEMENT</b></font></p></html><hr><br> |
- | + | <div align="center"> | |
+ | For a detailed correspondence between our BioBrick "wiki names" and submitted BioBrick codes take a look [https://2010.igem.org/Team:UNIPV-Pavia/Calendar/October/settimana3#October.2C_18th here]. | ||
+ | </div> | ||
<table class="cont" border=0> | <table class="cont" border=0> | ||
- | <tr><th>Biobrick | + | <tr> |
- | + | <th>Biobrick name</th> | |
- | <!-- | + | <th>Description</th> |
- | + | <th>Creation date</th> | |
- | + | <th>Strain</th> | |
+ | <th>Vector</th> | ||
+ | <th>Quality control</th> | ||
+ | <th>Length</th> | ||
+ | <th>Store</th> | ||
+ | </tr> | ||
+ | <!-- Parti e BB richiesti per l'iGEM - BioBrick 2010--> | ||
<tr> | <tr> | ||
<td> | <td> | ||
Line 28: | Line 38: | ||
<partinfo>pSB2K3</partinfo> | <partinfo>pSB2K3</partinfo> | ||
</td><td> | </td><td> | ||
- | Seq ok(MIT); orange pellet when contained in <partinfo>pSB2K3</partinfo> | + | Seq ok (MIT); orange pellet when contained in <partinfo>pSB2K3</partinfo> |
</td> | </td> | ||
<td>744 bp</td><td>BioBricks 2010</td> | <td>744 bp</td><td>BioBricks 2010</td> | ||
Line 45: | Line 55: | ||
<partinfo>J63009</partinfo> (Amp) | <partinfo>J63009</partinfo> (Amp) | ||
</td><td> | </td><td> | ||
- | Seq partially confirmed(MIT) because it lacks a T at the 3', but the ADH1 terminator (i.e. <partinfo>BBa_K165010</partinfo>) is ok. The correct ApE file is here. | + | Seq partially confirmed (MIT) because it lacks a T at the 3', but the ADH1 terminator (i.e. <partinfo>BBa_K165010</partinfo>) is ok. The correct ApE file is here. |
</td> | </td> | ||
<td>253 bp</td><td>BioBricks 2010</td> | <td>253 bp</td><td>BioBricks 2010</td> | ||
</tr> | </tr> | ||
- | |||
Line 64: | Line 73: | ||
<partinfo>pSB1AK3</partinfo> | <partinfo>pSB1AK3</partinfo> | ||
</td><td> | </td><td> | ||
- | Seq ok(MIT) | + | Seq ok (MIT). |
</td> | </td> | ||
<td>403 bp</td><td>BioBricks 2010</td> | <td>403 bp</td><td>BioBricks 2010</td> | ||
Line 82: | Line 91: | ||
<partinfo>pSB1A1</partinfo> | <partinfo>pSB1A1</partinfo> | ||
</td><td> | </td><td> | ||
- | Seq ok(MIT 2009); length ok | + | Seq ok (MIT 2009); length ok. |
</td> | </td> | ||
<td>769 bp</td><td>BioBricks 2010</td> | <td>769 bp</td><td>BioBricks 2010</td> | ||
Line 99: | Line 108: | ||
<partinfo>pSB1A2</partinfo> | <partinfo>pSB1A2</partinfo> | ||
</td><td> | </td><td> | ||
- | Seq ok(MIT) | + | Seq ok (MIT). |
</td> | </td> | ||
<td>406 bp</td><td>BioBricks 2010</td> | <td>406 bp</td><td>BioBricks 2010</td> | ||
Line 117: | Line 126: | ||
<partinfo>pSB1A2</partinfo> | <partinfo>pSB1A2</partinfo> | ||
</td><td> | </td><td> | ||
- | Seq ok(MIT); length ok | + | Seq ok (MIT); length ok. |
</td> | </td> | ||
<td>664 bp</td><td>BioBricks 2010</td> | <td>664 bp</td><td>BioBricks 2010</td> | ||
Line 134: | Line 143: | ||
<partinfo>pSB1A2</partinfo> | <partinfo>pSB1A2</partinfo> | ||
</td><td> | </td><td> | ||
- | Seq partially confirmed(MIT) for unknown reasons: the alignment is fully correct. The ApE file with the correct prefix and suffix is here. Length ok. | + | Seq partially confirmed (MIT) for unknown reasons: the alignment is fully correct. The ApE file with the correct prefix and suffix is here. Length ok. |
</td> | </td> | ||
<td>718 bp</td><td>BioBricks 2010</td> | <td>718 bp</td><td>BioBricks 2010</td> | ||
</tr> | </tr> | ||
+ | <tr> | ||
+ | <td> | ||
+ | <partinfo>BBa_K208001</partinfo> | ||
+ | <br/> | ||
+ | (stab-1) | ||
+ | </td><td> | ||
+ | phasin phaP<br/>(Silver Standard 5' and 3')</td> | ||
+ | <td> | ||
+ | 24/06/2010 | ||
+ | </td><td> | ||
+ | ''E. coli'' ??? | ||
+ | </td><td> | ||
+ | <partinfo>pSB3K3</partinfo> | ||
+ | </td><td> | ||
+ | Seq ok (BMR); length ok. | ||
+ | </td> | ||
+ | <td>579 bp</td><td>BioBricks 2010</td> | ||
+ | </tr> | ||
<tr> | <tr> | ||
<td> | <td> | ||
- | < | + | <partinfo>BBa_K208001</partinfo> |
+ | <br/> | ||
+ | (stab-2) | ||
</td><td> | </td><td> | ||
- | < | + | phasin phaP<br/>(Silver Standard 5' and 3')</td> |
+ | <td> | ||
+ | 24/06/2010 | ||
</td><td> | </td><td> | ||
- | + | ''E. coli'' ??? | |
</td><td> | </td><td> | ||
- | + | <partinfo>pSB3K3</partinfo> | |
</td><td> | </td><td> | ||
- | <partinfo> | + | Seq ok (BMR); length ok. |
+ | </td> | ||
+ | <td>579 bp</td><td>BioBricks 2010</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <partinfo>BBa_J72007</partinfo> | ||
+ | </td><td> | ||
+ | BamHI methyltransferase encoding CRIM plasmid | ||
+ | </td><td> | ||
+ | 01/07/2010 | ||
+ | </td><td> | ||
+ | ''E. coli'' ??? | ||
+ | </td><td> | ||
+ | HC Cm resistance | ||
</td><td> | </td><td> | ||
- | + | No plasmid in the clone.<br/> | |
+ | For more informations click <html><a href="https://2010.igem.org/Team:UNIPV-Pavia/Calendar/July/settimana3#July.2C_14th" target="_blank">here</a></html>. | ||
</td> | </td> | ||
- | <td></td><td> | + | <td></td><td>BioBricks 2010</td> |
</tr> | </tr> | ||
+ | <tr> | ||
+ | <td> | ||
+ | <partinfo>BBa_J72008</partinfo> | ||
+ | </td><td> | ||
+ | phi80 integration helper plasmid pInt80-649 | ||
+ | </td><td> | ||
+ | 01/07/2010 | ||
+ | </td><td> | ||
+ | ''E. coli'' ??? | ||
+ | </td><td> | ||
+ | Plasmid (Amp 50 ug/ml resistance) | ||
+ | </td><td> | ||
+ | Length ok. | ||
+ | </td> | ||
+ | <td>6335 bp</td><td>BioBricks 2010</td> | ||
+ | </tr> | ||
<tr> | <tr> | ||
<td> | <td> | ||
- | + | <partinfo>BBa_J72013</partinfo> | |
</td><td> | </td><td> | ||
- | + | BglII methyltransferase encoding CRIM plasmid | |
</td><td> | </td><td> | ||
- | + | 01/07/2010 | |
+ | </td><td> | ||
+ | ''E. coli'' ??? | ||
+ | </td><td> | ||
+ | HC Cm resistance | ||
+ | </td><td> | ||
+ | No plasmid in the clone.<br/> | ||
+ | For more informations click <html><a href="https://2010.igem.org/Team:UNIPV-Pavia/Calendar/July/settimana3#July.2C_15th" target="_blank">here</a></html>. | ||
+ | </td> | ||
+ | <td><!--2978 bp--></td> | ||
+ | <td>BioBricks 2010</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <partinfo>BBa_K105012</partinfo> | ||
+ | </td><td> | ||
+ | 10 aa flexible protein domain linker | ||
+ | </td><td> | ||
+ | 06/08/2010 | ||
</td><td> | </td><td> | ||
''E. coli'' DH5-alpha | ''E. coli'' DH5-alpha | ||
</td><td> | </td><td> | ||
- | <partinfo> | + | <partinfo>pSB1A2</partinfo> |
</td><td> | </td><td> | ||
- | + | - | |
</td> | </td> | ||
- | <td></td><td> | + | <td>30 bp</td> |
+ | <td>BioBricks 2010</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td> | <td> | ||
- | + | <partinfo>BBa_J13002</partinfo> | |
</td><td> | </td><td> | ||
- | <partinfo> | + | p(tetR)+RBS34 |
+ | </td> | ||
+ | <td> | ||
+ | 16/08/2010 | ||
+ | </td> | ||
+ | <td> | ||
+ | ''E. coli'' TOP10 | ||
+ | </td> | ||
+ | <td> | ||
+ | <partinfo>pSB1A2</partinfo> | ||
+ | </td> | ||
+ | <td> | ||
+ | - | ||
+ | </td> | ||
+ | <td>74 bp</td> | ||
+ | <td>BioBricks 2010</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <partinfo>BBa_I13522</partinfo> | ||
+ | </td><td> | ||
+ | Untagged GFP behind a constitutive promoter | ||
+ | </td> | ||
+ | <td> | ||
+ | 16/08/2010 | ||
+ | </td> | ||
+ | <td> | ||
+ | ''E. coli'' TOP10 | ||
+ | </td> | ||
+ | <td> | ||
+ | <partinfo>pSB1A2</partinfo> | ||
+ | </td> | ||
+ | <td> | ||
+ | - | ||
+ | </td> | ||
+ | <td>937 bp</td> | ||
+ | <td>BioBricks 2010</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">pSB1C3_RFP</font></td> | ||
+ | <td> | ||
+ | <partinfo>BBa_J04450</partinfo> <br/> in <partinfo>pSB1C3</partinfo>. | ||
+ | </td> | ||
+ | <td> | ||
+ | ?? | ||
+ | </td><td> | ||
+ | ''E. coli'' DH5alpha | ||
+ | </td><td> | ||
+ | <partinfo>pSB1C3</partinfo> | ||
+ | </td> | ||
+ | <td> | ||
+ | |||
+ | </td> | ||
+ | <td> | ||
+ | 1069 bp | ||
+ | </td> | ||
+ | <td>2009 Distribution</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">pSB1C3_ccdB</font></td> | ||
+ | <td> | ||
+ | <partinfo>BBa_P1010</partinfo> <br/> in <partinfo>pSB1C3</partinfo>. | ||
+ | </td> | ||
+ | <td> | ||
+ | ?? | ||
+ | </td><td> | ||
+ | ''E. coli'' DH5alpha | ||
+ | </td><td> | ||
+ | <partinfo>pSB1C3</partinfo> | ||
+ | </td> | ||
+ | <td> | ||
+ | |||
+ | </td> | ||
+ | <td> | ||
+ | 675 bp | ||
+ | </td> | ||
+ | <td>2009 Distribution</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | <tr><td colspan="8"> </td></tr> | ||
+ | |||
+ | <!--Ligazioni fatte durante l'iGEM2010 --> | ||
+ | <tr> | ||
+ | <th>Name</th> | ||
+ | <th>Ligation</th> | ||
+ | <th>Creation date</th> | ||
+ | <th>Strain</th> | ||
+ | <th>Vector</th> | ||
+ | <th>Quality control</th> | ||
+ | <th>Length</th> | ||
+ | <th>Description</th> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">I0</font> | ||
+ | <br/>(it was I0-2) | ||
+ | <br/><partinfo>BBa_k300005</partinfo> | ||
+ | </td><td> | ||
+ | <partinfo>BBa_K125500</partinfo> (E-S)<br>+<br><partinfo>BBa_B0015</partinfo> (E-X) | ||
</td><td> | </td><td> | ||
11/06/2010 | 11/06/2010 | ||
Line 185: | Line 375: | ||
''E. coli'' DH5-alpha | ''E. coli'' DH5-alpha | ||
</td><td> | </td><td> | ||
- | <partinfo> | + | <partinfo>pSB1AK3</partinfo> |
</td><td> | </td><td> | ||
- | + | Seq ok (BMR); length ok. | |
</td> | </td> | ||
- | <td></td><td> | + | <td>854 bp</td> |
+ | <td>GFPs + TT</td> | ||
</tr> | </tr> | ||
Line 195: | Line 386: | ||
<tr> | <tr> | ||
<td> | <td> | ||
- | I1-2 | + | <font class="BBok">I1</font> |
+ | <br/> | ||
+ | (it was I1-2) | ||
+ | <br/><partinfo>BBa_k300006</partinfo> | ||
</td><td> | </td><td> | ||
<partinfo>BBa_E2050</partinfo> (E-S)<br>+<br><partinfo>BBa_K165018</partinfo> (E-X)</td><td> | <partinfo>BBa_E2050</partinfo> (E-S)<br>+<br><partinfo>BBa_K165018</partinfo> (E-X)</td><td> | ||
Line 204: | Line 398: | ||
<partinfo>J63009</partinfo> (Amp) | <partinfo>J63009</partinfo> (Amp) | ||
</td><td> | </td><td> | ||
- | Seq ok(BMR), with an additional barcode (25bp) after the stop codon of mOrange; length ok | + | Seq ok (BMR), with an additional barcode (25bp) after the stop codon of mOrange; length ok. |
</td> | </td> | ||
- | <td></td><td> | + | <td>1029 bp</td> |
+ | <td>mOrange + NSL-ADH1 terminator</td> | ||
</tr> | </tr> | ||
Line 212: | Line 407: | ||
<tr> | <tr> | ||
<td> | <td> | ||
- | I2-1 | + | <font class="BBok">I2</font> |
+ | <br/> | ||
+ | (it was I2-1) | ||
</td><td> | </td><td> | ||
<partinfo>BBa_P1004</partinfo> (E-S)<br>+<br><partinfo>BBa_B0015</partinfo> (E-X) | <partinfo>BBa_P1004</partinfo> (E-S)<br>+<br><partinfo>BBa_B0015</partinfo> (E-X) | ||
Line 223: | Line 420: | ||
<partinfo>pSB1AK3</partinfo> | <partinfo>pSB1AK3</partinfo> | ||
</td><td> | </td><td> | ||
- | Seq ok(BMR); Cm resistance ok; length ok. | + | Seq ok (BMR); Cm resistance ok; length ok. |
</td> | </td> | ||
- | <td></td><td> | + | <td>906 bp</td> |
+ | <td>Cm resistance + TT</td> | ||
</tr> | </tr> | ||
+ | |||
<tr> | <tr> | ||
<td> | <td> | ||
- | + | <font class="BBok">I3</font> | |
+ | <br/> | ||
+ | (it was I3-1) | ||
+ | <br/><partinfo>BBa_k300009</partinfo> | ||
</td><td> | </td><td> | ||
- | <partinfo> | + | <partinfo>BBa_K081008</partinfo> (E-S)<br>+<br><partinfo>BBa_B0015</partinfo> (E-X)</td> |
<td> | <td> | ||
11/06/2010 | 11/06/2010 | ||
Line 241: | Line 443: | ||
<partinfo>pSB1AK3</partinfo> | <partinfo>pSB1AK3</partinfo> | ||
</td><td> | </td><td> | ||
- | + | Seq TWO PLASMIDS IN THIS CLONE!(BMR), two overlapped traces; length ok. | |
</td> | </td> | ||
- | <td></td><td> | + | <td>801 bp</td> |
+ | <td>RBS + luxI + TT</td> | ||
</tr> | </tr> | ||
Line 249: | Line 452: | ||
<tr> | <tr> | ||
<td> | <td> | ||
- | + | <font class="BBok">I4</font> | |
+ | <br/> | ||
+ | (it was I4-2) | ||
</td><td> | </td><td> | ||
- | <partinfo> | + | <partinfo>BBa_K165037</partinfo> (S-P)<br/>+<br/>I1 (X-P)</td> |
<td> | <td> | ||
- | + | 18/06/2010 | |
</td><td> | </td><td> | ||
''E. coli'' DH5-alpha | ''E. coli'' DH5-alpha | ||
Line 259: | Line 464: | ||
<partinfo>pSB1AK3</partinfo> | <partinfo>pSB1AK3</partinfo> | ||
</td><td> | </td><td> | ||
- | Seq | + | Seq ok (BMR); length ok. |
</td> | </td> | ||
- | <td></td><td> | + | <td>1439 bp</td> |
+ | <td>pTEF + mOrange + NLS + terminator</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td> | <td> | ||
- | < | + | <font class="BBok">I5</font> |
+ | <br/> | ||
+ | (it was I5-1) | ||
+ | <br/><partinfo>BBa_k300008</partinfo> | ||
</td><td> | </td><td> | ||
- | + | I2 (E-S)<br>+<br><partinfo>BBa_J61001</partinfo> (E-X)</td> | |
<td> | <td> | ||
- | + | 18/06/2010 | |
</td><td> | </td><td> | ||
- | ''E. coli'' | + | ''E. coli'' TOP10 |
</td><td> | </td><td> | ||
- | <partinfo> | + | <partinfo>pSB1A2</partinfo> |
</td><td> | </td><td> | ||
- | + | Seq ok (BMR); Cm resistance ok; length ok. | |
</td> | </td> | ||
- | <td></td><td> | + | <td>1320 bp</td> |
+ | <td>Cm resistance + TT + R6K ori</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td> | <td> | ||
- | + | <font class="BBok">I6</font> | |
+ | <br/> | ||
+ | (it was I6-2) | ||
</td><td> | </td><td> | ||
- | + | I3 (E-S)<br>+<br><partinfo>BBa_T9002</partinfo> (E-X)</td> | |
<td> | <td> | ||
18/06/2010 | 18/06/2010 | ||
Line 291: | Line 503: | ||
''E. coli'' DH5-alpha | ''E. coli'' DH5-alpha | ||
</td><td> | </td><td> | ||
- | <partinfo> | + | <partinfo>pSB1A3</partinfo> |
</td><td> | </td><td> | ||
- | + | Seq LONG PART(BMR), but the ligation worked and the sequenced portions are ok; length ok. | |
</td> | </td> | ||
- | <td></td><td> | + | <td>2754 bp</td> |
+ | <td>RBS + luxI + TT + HSL sensor</td> | ||
</tr> | </tr> | ||
+ | |||
<tr> | <tr> | ||
<td> | <td> | ||
- | + | <font class="BBok">I7</font> | |
+ | <br/> | ||
+ | (it was I7-3) | ||
+ | <br/><partinfo>BBa_k300024</partinfo> | ||
</td><td> | </td><td> | ||
- | <partinfo> | + | <partinfo>BBa_J23118</partinfo> (S-P)<br/>+<br/>I6 (X-P)</td> |
<td> | <td> | ||
- | + | 25/06/2010 | |
+ | </td><td> | ||
+ | ''E. coli'' TOP10 | ||
+ | </td><td> | ||
+ | <partinfo>BBa_J61002</partinfo><br/> | ||
+ | (Amp) | ||
+ | </td><td> | ||
+ | Length ok. | ||
+ | </td> | ||
+ | <td>2795 bp</td> | ||
+ | <td>constitutive promoter + RBS + luxI + TT + HSL sensor</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">I8</font> | ||
+ | <br/> | ||
+ | (it was I8-5) | ||
+ | <br/><partinfo>BBa_K300021</partinfo> | ||
+ | </td><td> | ||
+ | <partinfo>BBa_J23110</partinfo> (S-P)<br/>+<br/>I6 (X-P)</td> | ||
+ | <td> | ||
+ | 25/06/2010 | ||
+ | </td><td> | ||
+ | ''E. coli'' TOP10 | ||
+ | </td><td> | ||
+ | <partinfo>BBa_J61002</partinfo><br/> | ||
+ | (Amp) | ||
+ | </td><td> | ||
+ | Length ok. | ||
+ | </td> | ||
+ | <td>2795 bp</td> | ||
+ | <td>constitutive promoter + RBS + luxI + TT + HSL sensor</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">I9</font> | ||
+ | <br/> | ||
+ | (it was I9-1) | ||
+ | <br/><partinfo>BBa_K300022</partinfo> | ||
+ | </td><td> | ||
+ | <partinfo>BBa_J23114</partinfo> (S-P)<br/>+<br/>I6 (X-P)</td> | ||
+ | <td> | ||
+ | 25/06/2010 | ||
+ | </td><td> | ||
+ | ''E. coli'' TOP10 | ||
+ | </td><td> | ||
+ | <partinfo>BBa_J61002</partinfo><br/> | ||
+ | (Amp) | ||
+ | </td><td> | ||
+ | Length ok. | ||
+ | </td> | ||
+ | <td>2795 bp</td> | ||
+ | <td>constitutive promoter + RBS + luxI + TT + HSL sensor</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">I10</font> | ||
+ | <br/> | ||
+ | (it was I10-1) | ||
+ | <br/><partinfo>BBa_K300023</partinfo> | ||
+ | </td><td> | ||
+ | <partinfo>BBa_J23116</partinfo> (S-P)<br/>+<br/>I6 (X-P)</td> | ||
+ | <td> | ||
+ | 25/06/2010 | ||
+ | </td><td> | ||
+ | ''E. coli'' TOP10 | ||
+ | </td><td> | ||
+ | <partinfo>BBa_J61002</partinfo><br/> | ||
+ | (Amp) | ||
+ | </td><td> | ||
+ | Length ok. | ||
+ | </td> | ||
+ | <td>2795 bp</td> | ||
+ | <td>constitutive promoter + RBS + luxI + TT + HSL sensor</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">I12</font> | ||
+ | <br/> | ||
+ | (it was I12-2) | ||
+ | <br/><partinfo>BBa_k300019</partinfo> | ||
+ | </td> | ||
+ | <td> | ||
+ | <partinfo>BBa_J23105</partinfo> (S-P)<br/>+<br/>I6 (X-P) | ||
+ | </td> | ||
+ | <td> | ||
+ | 16/07/2010 | ||
+ | </td><td> | ||
+ | ''E. coli'' TOP10 | ||
+ | </td><td> | ||
+ | <partinfo>BBa_J61002</partinfo><br/> | ||
+ | (Amp) | ||
+ | </td><td> | ||
+ | Seq ok (BMR), length ok. | ||
+ | </td> | ||
+ | <td>2795 bp</td> | ||
+ | <td>constitutive promoter + RBS + luxI + TT + HSL sensor</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">I14</font> | ||
+ | <br/> | ||
+ | (it was I14-1) | ||
+ | <br/><partinfo>BBa_k300030</partinfo> | ||
+ | </td> | ||
+ | <td> | ||
+ | <partinfo>BBa_J23118</partinfo> (S-P)<br/>+<br/>I3 (X-P) | ||
+ | </td> | ||
+ | <td> | ||
+ | 16/07/2010 | ||
</td><td> | </td><td> | ||
''E. coli'' DH5-alpha | ''E. coli'' DH5-alpha | ||
</td><td> | </td><td> | ||
+ | <partinfo>BBa_J61002</partinfo><br/> | ||
+ | (Amp) | ||
+ | </td><td> | ||
+ | Seq ok (BMR), length ok. | ||
+ | </td> | ||
+ | <td>842 bp</td> | ||
+ | <td>constitutive promoter + RBS + luxI + TT</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">RING</font> | ||
+ | <br/> | ||
+ | </td> | ||
+ | <td> | ||
+ | I5 (X-S) on itself:<br/> | ||
+ | Cm resistance (<partinfo>BBa_P1004</partinfo>) + TT (<partinfo>BBa_B0015</partinfo>) + R6K ori (<partinfo>BBa_J61001</partinfo>) | ||
+ | </td> | ||
+ | <td> | ||
+ | 16/07/2010 | ||
+ | </td><td> | ||
+ | ''E. coli'' DH5-alpha | ||
+ | </td><td> | ||
+ | Plasmid (Cm resistance, conditional thermosensitive ORI; works in PIR strains) | ||
+ | </td><td> | ||
+ | Tested <html><a href="https://2010.igem.org/Team:UNIPV-Pavia/Calendar/July/settimana4#July.2C_19th" target="_blank">here</a></html>. | ||
+ | </td> | ||
+ | <td> | ||
+ | 1328 bp | ||
+ | </td> | ||
+ | <td>Cm resistance + TT + R6K ori</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">I15</font> | ||
+ | <br/> | ||
+ | (it was I15-1) | ||
+ | <br/><partinfo>BBa_k300028</partinfo> | ||
+ | </td> | ||
+ | <td> | ||
+ | <partinfo>BBa_J23110</partinfo> (S-P)<br/>+<br/>I3 (X-P) | ||
+ | </td> | ||
+ | <td> | ||
+ | 23/07/2010 | ||
+ | </td><td> | ||
+ | ''E. coli'' DH5-alpha | ||
+ | </td><td> | ||
+ | <partinfo>BBa_J61002</partinfo><br/> | ||
+ | (Amp) | ||
+ | </td><td> | ||
+ | Length ok. | ||
+ | </td> | ||
+ | <td>842 bp</td> | ||
+ | <td>constitutive promoter + RBS + luxI + TT</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">I16</font> | ||
+ | <br/> | ||
+ | (it was I16-1) | ||
+ | <br/><partinfo>BBa_k300029</partinfo> | ||
+ | </td> | ||
+ | <td> | ||
+ | <partinfo>BBa_J23116</partinfo> (S-P)<br/>+<br/>I3 (X-P) | ||
+ | </td> | ||
+ | <td> | ||
+ | 16/07/2010 | ||
+ | </td><td> | ||
+ | ''E. coli'' DH5-alpha | ||
+ | </td><td> | ||
+ | <partinfo>BBa_J61002</partinfo><br/> | ||
+ | (Amp) | ||
+ | </td><td> | ||
+ | Seq ok (BMR), length ok. | ||
+ | </td> | ||
+ | <td>842 bp</td> | ||
+ | <td>constitutive promoter + RBS + luxI + TT</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">I17</font> | ||
+ | <br/> | ||
+ | (it was I17-1) | ||
+ | <br/><partinfo>BBa_k300025</partinfo> | ||
+ | </td> | ||
+ | <td> | ||
+ | <partinfo>BBa_J23101</partinfo> (S-P)<br/>+<br/>I3 (X-P) | ||
+ | </td> | ||
+ | <td> | ||
+ | 16/07/2010 | ||
+ | </td><td> | ||
+ | ''E. coli'' DH5-alpha | ||
+ | </td><td> | ||
+ | <partinfo>BBa_J61002</partinfo><br/> (Amp) | ||
+ | </td><td> | ||
+ | Seq ok (BMR), length ok. | ||
+ | </td> | ||
+ | <td>842 bp</td> | ||
+ | <td>constitutive promoter + RBS + luxI + TT</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">I18</font> | ||
+ | <br/> | ||
+ | (it was I18-1) | ||
+ | <br/><partinfo>BBa_k300026</partinfo> | ||
+ | </td> | ||
+ | <td> | ||
+ | <partinfo>BBa_J23105</partinfo> (S-P)<br/>+<br/>I3 (X-P) | ||
+ | </td> | ||
+ | <td> | ||
+ | 16/07/2010 | ||
+ | </td><td> | ||
+ | ''E. coli'' DH5-alpha | ||
+ | </td><td> | ||
+ | <partinfo>BBa_J61002</partinfo><br/> | ||
+ | (Amp) | ||
+ | </td><td> | ||
+ | Seq ok (BMR), length ok. | ||
+ | </td> | ||
+ | <td>842 bp</td> | ||
+ | <td>constitutive promoter + RBS + luxI + TT</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">I19</font> | ||
+ | <br/> | ||
+ | (it was I19-1) | ||
+ | <br/><partinfo>BBa_k300027</partinfo> | ||
+ | </td> | ||
+ | <td> | ||
+ | <partinfo>BBa_J23106</partinfo> (S-P)<br/>+<br/>I3 (X-P) | ||
+ | </td> | ||
+ | <td> | ||
+ | 16/07/2010 | ||
+ | </td><td> | ||
+ | ''E. coli'' DH5-alpha | ||
+ | </td><td> | ||
+ | <partinfo>BBa_J61002</partinfo><br/> | ||
+ | (Amp) | ||
+ | </td><td> | ||
+ | Seq ok (BMR), length ok. | ||
+ | </td> | ||
+ | <td>842 bp</td> | ||
+ | <td>constitutive promoter + RBS + luxI + TT</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">I20</font> | ||
+ | <br/> | ||
+ | (it was I20M-1) | ||
+ | <br/><partinfo>BBa_k300002</partinfo> | ||
+ | </td> | ||
+ | <td> | ||
+ | Pha10S-1 (X-S) (infos <html><a href="https://2010.igem.org/Team:UNIPV-Pavia/Calendar/July/settimana5#July.2C_29th" target="_blank">here</a></html>) <br/>+<br/> <partinfo>pSB1AK3</partinfo> (X-S) | ||
+ | </td> | ||
+ | <td> | ||
+ | 13/08/2010 | ||
+ | </td> | ||
+ | <td> | ||
+ | ''E. coli'' DH5-alpha | ||
+ | </td> | ||
+ | <td> | ||
<partinfo>pSB1AK3</partinfo> | <partinfo>pSB1AK3</partinfo> | ||
+ | </td> | ||
+ | <td> | ||
+ | Seq ok (BMR), length ok. | ||
+ | </td> | ||
+ | <td> | ||
+ | 576 bp | ||
+ | </td> | ||
+ | <td>Pha10S</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">I21</font> | ||
+ | <br/> | ||
+ | (it was I21-4) | ||
+ | <br/><partinfo>BBa_k300003</partinfo> | ||
+ | </td> | ||
+ | <td> | ||
+ | PhaSS-1 (X-S) (infos <html><a href="https://2010.igem.org/Team:UNIPV-Pavia/Calendar/August/settimana2#August.2C_10th" target="_blank">here</a></html>) <br/>+<br/> <partinfo>pSB1A3</partinfo> (X-S) | ||
+ | </td> | ||
+ | <td> | ||
+ | 10/08/2010 | ||
</td><td> | </td><td> | ||
- | Seq ok(BMR) | + | ''E. coli'' DH5-alpha |
+ | </td><td> | ||
+ | <partinfo>pSB1A3</partinfo> | ||
+ | </td><td> | ||
+ | Seq ok (BMR), length ok. | ||
</td> | </td> | ||
- | <td></td><td> | + | <td> |
+ | 576 bp | ||
+ | </td> | ||
+ | <td>PhaSS</td> | ||
</tr> | </tr> | ||
+ | |||
<tr> | <tr> | ||
<td> | <td> | ||
- | + | <font class="BBok">I22</font> | |
+ | <br/> | ||
+ | (it was I22-1) | ||
+ | <br/><partinfo>BBa_k300031</partinfo> | ||
+ | </td> | ||
+ | <td> | ||
+ | <partinfo>BBa_K081009</partinfo> (E-S) <br/>+<br/> <partinfo>BBa_B0015</partinfo> (E-X) | ||
+ | </td> | ||
+ | <td> | ||
+ | 10/08/2010 | ||
</td><td> | </td><td> | ||
- | + | ''E. coli'' DH5-alpha | |
+ | </td><td> | ||
+ | <partinfo>pSB1AK3</partinfo> | ||
+ | </td><td> | ||
+ | Length ok. | ||
+ | </td> | ||
<td> | <td> | ||
- | + | 825 bp | |
+ | </td> | ||
+ | <td>RBS + lasI + terminator</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">I23</font> | ||
+ | <br/> | ||
+ | (it was I23-1) | ||
+ | </td> | ||
+ | <td> | ||
+ | <partinfo>BBa_R0062</partinfo> (S-P) <br/>+<br/> I3 (X-P) | ||
+ | </td> | ||
+ | <td> | ||
+ | 10/08/2010 | ||
</td><td> | </td><td> | ||
''E. coli'' DH5-alpha | ''E. coli'' DH5-alpha | ||
Line 327: | Line 899: | ||
<partinfo>pSB1A2</partinfo> | <partinfo>pSB1A2</partinfo> | ||
</td><td> | </td><td> | ||
- | + | Length ok. | |
</td> | </td> | ||
- | <td></td><td> | + | <td> |
+ | 725 bp | ||
+ | </td> | ||
+ | <td>plux + RBS + luxI + TT</td> | ||
</tr> | </tr> | ||
+ | |||
<tr> | <tr> | ||
<td> | <td> | ||
- | + | <font class="BBok">I24</font> | |
+ | <br/> | ||
+ | (it was I24-1) | ||
+ | </td> | ||
+ | <td> | ||
+ | <partinfo>BBa_R0062</partinfo> (S-P) <br/>+<br/> I22 (X-P) | ||
+ | </td> | ||
+ | <td> | ||
+ | 13/08/2010 | ||
</td><td> | </td><td> | ||
- | + | ''E. coli'' DH5-alpha | |
+ | </td> | ||
<td> | <td> | ||
- | + | <partinfo>pSB1A2</partinfo> | |
+ | </td> | ||
+ | <td> | ||
+ | Length ok. | ||
+ | </td> | ||
+ | <td> | ||
+ | 886 bp | ||
+ | </td> | ||
+ | <td>plux + RBS + lasI + terminator</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">I26</font> | ||
+ | <br/> | ||
+ | (it was I26-2) | ||
+ | <br/><partinfo>BBa_k300081</partinfo> | ||
+ | </td> | ||
+ | <td> | ||
+ | <partinfo>BBa_K105012</partinfo> (S-P) <br/>+<br/> I21 (X-P) | ||
+ | </td> | ||
+ | <td> | ||
+ | 13/08/2010 | ||
</td><td> | </td><td> | ||
''E. coli'' DH5-alpha | ''E. coli'' DH5-alpha | ||
+ | </td> | ||
+ | <td> | ||
+ | <partinfo>pSB1A2</partinfo> | ||
+ | </td> | ||
+ | <td> | ||
+ | Seq ok (BMR), length ok. | ||
+ | </td> | ||
+ | <td> | ||
+ | 612 bp | ||
+ | </td> | ||
+ | <td>linker + PhaSS</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">I29</font> | ||
+ | <br/> | ||
+ | (it was I29-1) | ||
+ | </td> | ||
+ | <td> | ||
+ | <partinfo>BBa_T9002</partinfo> (S-P) <br/>+<br/> I23 (X-P) | ||
+ | </td> | ||
+ | <td> | ||
+ | 31/08/2010 | ||
+ | </td> | ||
+ | <td> | ||
+ | ''E. coli'' TOP10 | ||
+ | </td> | ||
+ | <td> | ||
+ | <partinfo>pSB1A3</partinfo> | ||
+ | </td> | ||
+ | <td> | ||
+ | Length ok. | ||
+ | </td> | ||
+ | <td> | ||
+ | 2678 bp | ||
+ | </td> | ||
+ | <td>HLS sensor + plux + RBS + luxI + TT</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | I30 | ||
+ | </td> | ||
+ | <td> | ||
+ | <partinfo>BBa_T9002</partinfo> (S-P) <br/>+<br/> I24 (X-P) | ||
+ | </td> | ||
+ | <td> | ||
+ | |||
+ | </td> | ||
+ | <td> | ||
+ | ''E. coli'' TOP10 | ||
+ | </td> | ||
+ | <td> | ||
+ | <partinfo>pSB1A3</partinfo> | ||
+ | </td> | ||
+ | <td> | ||
+ | |||
+ | </td> | ||
+ | <td> | ||
+ | 2839 bp | ||
+ | </td> | ||
+ | <td>HSL sensor + plux + RBS + lasI + terminator</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">I31</font> | ||
+ | <br/> | ||
+ | (it was I31-1) | ||
+ | <br/><partinfo>BBa_K300079</partinfo> | ||
+ | </td> | ||
+ | <td> | ||
+ | I20 (E-S) <br/>+<br/> <partinfo>BBa_K105012</partinfo> (E-X) | ||
+ | </td> | ||
+ | <td> | ||
+ | 23/08/2010 | ||
+ | </td> | ||
+ | <td> | ||
+ | ''E. coli'' DH5-alpha | ||
+ | </td> | ||
+ | <td> | ||
+ | <partinfo>pSB1A2</partinfo> | ||
+ | </td> | ||
+ | <td> | ||
+ | Seq ok (BMR), length ok. | ||
+ | </td> | ||
+ | <td> | ||
+ | 612 bp | ||
+ | </td> | ||
+ | <td>Pha10S + linker</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">I32</font> | ||
+ | <br/> | ||
+ | (it was I32-2) | ||
+ | </td> | ||
+ | <td> | ||
+ | I20 (E-S) <br/>+<br/> I21 (E-X) | ||
+ | </td> | ||
+ | <td> | ||
+ | 23/08/2010 | ||
</td><td> | </td><td> | ||
+ | ''E. coli'' DH5-alpha | ||
+ | </td> | ||
+ | <td> | ||
<partinfo>pSB1A3</partinfo> | <partinfo>pSB1A3</partinfo> | ||
+ | </td> | ||
+ | <td> | ||
+ | Seq ok (BMR), length ok. | ||
+ | </td> | ||
+ | <td> | ||
+ | 1158 bp | ||
+ | </td> | ||
+ | <td>Pha10S + PhaSS</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">I33</font> | ||
+ | <br/> | ||
+ | (it was I33-1) | ||
+ | </td> | ||
+ | <td> | ||
+ | I20 (E-S) <br/>+<br/> I0 (E-X) | ||
+ | </td> | ||
+ | <td> | ||
+ | 23/08/2010 | ||
</td><td> | </td><td> | ||
- | Seq | + | ''E. coli'' DH5-alpha |
+ | </td> | ||
+ | <td> | ||
+ | <partinfo>pSB1AK3</partinfo> | ||
+ | </td> | ||
+ | <td> | ||
+ | Seq ok (BMR), length ok. | ||
</td> | </td> | ||
- | <td></td><td> | + | <td> |
+ | 1436 bp | ||
+ | </td> | ||
+ | <td>Pha10S + GFPs + TT</td> | ||
</tr> | </tr> | ||
+ | |||
<tr> | <tr> | ||
<td> | <td> | ||
- | + | <font class="BBok">I34</font> | |
+ | <br/> | ||
+ | (it was I34-1) | ||
+ | </td> | ||
+ | <td> | ||
+ | I21 (E-S) <br/>+<br/> I0 (E-X) | ||
+ | </td> | ||
+ | <td> | ||
+ | 23/08/2010 | ||
</td><td> | </td><td> | ||
- | + | ''E. coli'' DH5-alpha | |
+ | </td> | ||
<td> | <td> | ||
- | + | <partinfo>pSB1AK3</partinfo> | |
+ | </td> | ||
+ | <td> | ||
+ | Seq ok (BMR), length ok. | ||
+ | </td> | ||
+ | <td> | ||
+ | 1436 bp | ||
+ | </td> | ||
+ | <td>PhaSS + GFPs + TT</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">I35</font> | ||
+ | <br/> | ||
+ | (it was I35-1) | ||
+ | <br/><partinfo>BBa_K300080</partinfo> | ||
+ | </td> | ||
+ | <td> | ||
+ | I21 (E-S) <br/>+<br/> <partinfo>BBa_K105012</partinfo> (E-X) | ||
+ | </td> | ||
+ | <td> | ||
+ | 23/08/2010 | ||
</td><td> | </td><td> | ||
''E. coli'' DH5-alpha | ''E. coli'' DH5-alpha | ||
+ | </td> | ||
+ | <td> | ||
+ | <partinfo>pSB1A2</partinfo> | ||
+ | </td> | ||
+ | <td> | ||
+ | Seq ok (BMR), length ok. | ||
+ | </td> | ||
+ | <td> | ||
+ | 612 bp | ||
+ | </td> | ||
+ | <td>PhaSS + linker</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">I36</font> | ||
+ | <br/> | ||
+ | (it was I36-7) | ||
+ | </td> | ||
+ | <td> | ||
+ | I21 (E-S) <br/>+<br/> I21 (E-X) | ||
+ | </td> | ||
+ | <td> | ||
+ | 24/08/2010 | ||
</td><td> | </td><td> | ||
+ | ''E. coli'' DH5-alpha | ||
+ | </td> | ||
+ | <td> | ||
<partinfo>pSB1A3</partinfo> | <partinfo>pSB1A3</partinfo> | ||
+ | </td> | ||
+ | <td> | ||
+ | Length ok. | ||
+ | </td> | ||
+ | <td> | ||
+ | 1158 bp | ||
+ | </td> | ||
+ | <td>PhaSS + PhaSS</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">I37</font> | ||
+ | <br/> | ||
+ | (it was I37-1) | ||
+ | </td> | ||
+ | <td> | ||
+ | INTEIN (E-S) <br/>+<br/> I0 (E-X) | ||
+ | </td> | ||
+ | <td> | ||
+ | 23/08/2010 | ||
</td><td> | </td><td> | ||
- | + | ''E. coli'' DH5-alpha | |
+ | </td> | ||
+ | <td> | ||
+ | <partinfo>pSB1AK3</partinfo> | ||
+ | </td> | ||
+ | <td> | ||
+ | Seq ok (BMR), length ok. | ||
</td> | </td> | ||
- | <td></td><td> | + | <td> |
+ | 1367 bp | ||
+ | </td> | ||
+ | <td>INTEIN + GFPs + TT</td> | ||
</tr> | </tr> | ||
+ | |||
<tr> | <tr> | ||
<td> | <td> | ||
- | <partinfo> | + | <font class="BBok">I38</font> |
+ | <br/> | ||
+ | (it was I38-3) | ||
+ | </td> | ||
+ | <td> | ||
+ | <partinfo>BBa_F2620</partinfo> (E-S) <br/>+<br/> <partinfo>BBa_B0034</partinfo> (E-X) | ||
+ | </td> | ||
+ | <td> | ||
+ | 23/08/2010 | ||
</td><td> | </td><td> | ||
- | + | ''E. coli'' DH5-alpha | |
+ | </td> | ||
<td> | <td> | ||
- | 24/ | + | <partinfo>pSB1A2</partinfo> |
+ | </td> | ||
+ | <td> | ||
+ | Seq ok (BMR), length ok. | ||
+ | </td> | ||
+ | <td> | ||
+ | 1081 bp | ||
+ | </td> | ||
+ | <td>HSL inducible system</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">I39</font> | ||
+ | <br/> | ||
+ | (it was I39-5) | ||
+ | </td> | ||
+ | <td> | ||
+ | <partinfo>BBa_J13002</partinfo> (S-P) <br/>+<br/> I20 (X-P) | ||
+ | </td> | ||
+ | <td> | ||
+ | 24/08/2010 | ||
</td><td> | </td><td> | ||
- | ''E. coli'' | + | ''E. coli'' DH5-alpha |
+ | </td> | ||
+ | <td> | ||
+ | <partinfo>pSB1A2</partinfo> | ||
+ | </td> | ||
+ | <td> | ||
+ | Seq ok (BMR), length ok. | ||
+ | </td> | ||
+ | <td> | ||
+ | 656 bp | ||
+ | </td> | ||
+ | <td>pTet + Pha10S</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">I40</font> | ||
+ | <br/> | ||
+ | (it was I40-1) | ||
+ | </td> | ||
+ | <td> | ||
+ | I26 (E-S) <br/>+<br/> INTEIN (E-X) | ||
+ | </td> | ||
+ | <td> | ||
+ | 25/08/2010 | ||
</td><td> | </td><td> | ||
- | < | + | ''E. coli'' DH5-alpha |
+ | </td> | ||
+ | <td> | ||
+ | pMA (HC Amp resistance) | ||
+ | </td> | ||
+ | <td> | ||
+ | Length ok. | ||
+ | </td> | ||
+ | <td> | ||
+ | 1125 bp | ||
+ | </td> | ||
+ | <td>linker + PhaSS + INTEIN</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">I41</font> | ||
+ | <br/> | ||
+ | (it was I41-1) | ||
+ | </td> | ||
+ | <td> | ||
+ | I26 (E-S) <br/>+<br/> I0 (E-X) | ||
+ | </td> | ||
+ | <td> | ||
+ | 23/08/2010 | ||
</td><td> | </td><td> | ||
- | + | ''E. coli'' DH5-alpha | |
+ | </td> | ||
+ | <td> | ||
+ | <partinfo>pSB1AK3</partinfo> | ||
+ | </td> | ||
+ | <td> | ||
+ | Seq ok (BMR), length ok. | ||
</td> | </td> | ||
- | <td> | + | <td> |
+ | 1472 bp | ||
+ | </td> | ||
+ | <td>linker + PhaSS + GFPs + TT</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td> | <td> | ||
- | <partinfo> | + | <font class="BBok">I42</font><br/> |
+ | (it was I42-3) | ||
+ | <br/> | ||
+ | </td> | ||
+ | <td> | ||
+ | CREAM (E-P) <br/>+<br/> <partinfo>BBa_K173001</partinfo> (E-P) | ||
+ | </td> | ||
+ | <td> | ||
+ | 23/08/2010 | ||
</td><td> | </td><td> | ||
- | + | ''E. coli'' BW23474 | |
+ | </td> | ||
<td> | <td> | ||
- | + | CREAM | |
+ | </td> | ||
+ | <td> | ||
+ | Length ok. | ||
+ | </td> | ||
+ | <td> | ||
+ | 3068 bp | ||
+ | </td> | ||
+ | <td>CREAM fragment + Cm resistance + TT + R6K ori + constitutive GFP</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">I43</font><br/><br/> | ||
+ | (it was I43-2) | ||
+ | <br/> | ||
+ | </td> | ||
+ | <td> | ||
+ | CREAM (E-P) <br/>+<br/><partinfo>BBa_J23101</partinfo> (E-P) | ||
+ | </td> | ||
+ | <td> | ||
+ | 23/08/2010 | ||
</td><td> | </td><td> | ||
- | ''E. coli'' | + | ''E. coli'' BW23474 |
+ | </td> | ||
+ | <td> | ||
+ | CREAM | ||
+ | </td> | ||
+ | <td> | ||
+ | Length ok. | ||
+ | </td> | ||
+ | <td> | ||
+ | 3026 bp | ||
+ | </td> | ||
+ | <td>CREAM fragment + Cm resistance + TT + R6K ori + constitutive promoter</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">I44</font> | ||
+ | <br/> | ||
+ | (it was I44-2) | ||
+ | <br/> | ||
+ | </td> | ||
+ | <td> | ||
+ | CREAM (E-P) <br/>+<br/><partinfo>BBa_I52002</partinfo> (E-P) | ||
+ | </td> | ||
+ | <td> | ||
+ | 15/09/2010 | ||
</td><td> | </td><td> | ||
- | < | + | ''E. coli'' DB3.1 |
+ | </td> | ||
+ | <td> | ||
+ | CREAM | ||
+ | </td> | ||
+ | <td> | ||
+ | Seq ok (BMR). | ||
+ | </td> | ||
+ | <td> | ||
+ | 3239 bp | ||
+ | </td> | ||
+ | <td>CREAM fragment + Cm resistance + TT + R6K ori + marker ccdB</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | I45<br/> | ||
+ | FAILED | ||
+ | <br/> | ||
+ | </td> | ||
+ | <td> | ||
+ | CREAM (HindIII) on itself | ||
+ | </td> | ||
+ | <td> | ||
+ | 23/08/2010 | ||
</td><td> | </td><td> | ||
- | + | ''E. coli'' BW23474 | |
+ | </td> | ||
+ | <td> | ||
+ | CREAM | ||
+ | </td> | ||
+ | <td> | ||
+ | |||
</td> | </td> | ||
- | <td> | + | <td> |
+ | 2149 bp | ||
+ | </td> | ||
+ | <td>CREAM fragment + Cm resistance + TT + R6K ori</td> | ||
</tr> | </tr> | ||
+ | |||
<tr> | <tr> | ||
<td> | <td> | ||
- | + | <font class="BBok">I47</font> | |
+ | <br/> | ||
+ | (it was I47-13) | ||
+ | <br/><partinfo>BBa_K300086</partinfo> | ||
+ | <br/> | ||
+ | </td> | ||
+ | <td> | ||
+ | <partinfo>BBa_J13002</partinfo> (S-P) + I33 (X-P) | ||
+ | </td> | ||
+ | <td> | ||
+ | 02/09/2010 | ||
</td><td> | </td><td> | ||
- | + | ''E. coli'' TOP10 | |
+ | </td> | ||
<td> | <td> | ||
- | + | <partinfo>pSB1A2</partinfo> | |
+ | </td> | ||
+ | <td> | ||
+ | Seq ok (BMR), length ok. | ||
+ | </td> | ||
+ | <td> | ||
+ | 1516 bp | ||
+ | </td> | ||
+ | <td>pTet + Pha10S + GFPs + TT</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">I48</font> | ||
+ | <br/> | ||
+ | (it was I48-1) | ||
+ | <br/><partinfo>BBa_K300088</partinfo> | ||
+ | <br/> | ||
+ | </td> | ||
+ | <td> | ||
+ | I39 (S-P) + I34 (X-P) | ||
+ | </td> | ||
+ | <td> | ||
+ | 31/08/2010 | ||
+ | </td><td> | ||
+ | ''E. coli'' TOP10 | ||
+ | </td> | ||
+ | <td> | ||
+ | <partinfo>pSB1A2</partinfo> | ||
+ | </td> | ||
+ | <td> | ||
+ | Seq ok (BMR), length ok. | ||
+ | </td> | ||
+ | <td> | ||
+ | 2098 bp | ||
+ | </td> | ||
+ | <td>pTet + Pha10S + PhaSS + GFPs + TT</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">I49</font> | ||
+ | <br/> | ||
+ | (it was I49-1) | ||
+ | <br/><partinfo>BBa_K300090</partinfo> | ||
+ | <br/> | ||
+ | </td> | ||
+ | <td> | ||
+ | I39 (S-P) + I41 (X-P) | ||
+ | </td> | ||
+ | <td> | ||
+ | 31/08/2010 | ||
+ | </td><td> | ||
+ | ''E. coli'' TOP10 | ||
+ | </td> | ||
+ | <td> | ||
+ | <partinfo>pSB1A2</partinfo> | ||
+ | </td> | ||
+ | <td> | ||
+ | Seq ok (BMR), length ok. | ||
+ | </td> | ||
+ | <td> | ||
+ | 2134 bp | ||
+ | </td> | ||
+ | <td>pTet + Pha10S + linker + PhaSS + GFPs + TT</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | I50<br/> | ||
+ | FAILED (no trasformants on plate) | ||
+ | <br/> | ||
+ | </td> | ||
+ | <td> | ||
+ | I44 (E-H) on itself | ||
+ | </td> | ||
+ | <td> | ||
+ | 28/08/2010 | ||
+ | </td><td> | ||
+ | ''E. coli'' DB3.1 | ||
+ | </td> | ||
+ | <td> | ||
+ | CREAM | ||
+ | </td> | ||
+ | <td> | ||
+ | |||
+ | </td> | ||
+ | <td> | ||
+ | 3239 bp | ||
+ | </td> | ||
+ | <td>CREAM fragment + Cm resistance + TT + R6K ori + marker ccdB</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">I51</font> | ||
+ | <br/> | ||
+ | (it was I51-3) | ||
+ | <br/><partinfo>BBa_I52002</partinfo> | ||
+ | <br/> | ||
+ | </td> | ||
+ | <td> | ||
+ | CREAM (E-P) <br/>+<br/><partinfo>BBa_I52002</partinfo> (E-P) | ||
+ | </td> | ||
+ | <td> | ||
+ | 03/09/2010 | ||
+ | </td><td> | ||
+ | ''E. coli'' DB3.1 | ||
+ | </td> | ||
+ | <td> | ||
+ | CREAM | ||
+ | </td> | ||
+ | <td> | ||
+ | |||
+ | </td> | ||
+ | <td> | ||
+ | 3239 bp | ||
+ | </td> | ||
+ | <td>CREAM fragment + Cm resistance + TT + R6K ori + marker ccdB</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">I52</font> | ||
+ | <br/> | ||
+ | (it was I52-c) | ||
+ | <br/> | ||
+ | </td> | ||
+ | <td> | ||
+ | I35 (S-P)<br/>+<br/>I37 (X-P) | ||
+ | </td> | ||
+ | <td> | ||
+ | 17/09/2010 | ||
</td><td> | </td><td> | ||
''E. coli'' DH5-alpha | ''E. coli'' DH5-alpha | ||
+ | </td> | ||
+ | <td> | ||
+ | <partinfo>pSB1A2</partinfo> | ||
+ | </td> | ||
+ | <td> | ||
+ | Length ok. | ||
+ | </td> | ||
+ | <td> | ||
+ | 1985 bp | ||
+ | </td> | ||
+ | <td>PhaSS + linker + INTEIN + GFPs + TT</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">I53</font> | ||
+ | <br/> | ||
+ | (it was I53-c) | ||
+ | <br/> | ||
+ | </td> | ||
+ | <td> | ||
+ | I32 (E-S)<br/>+<br/>I37 (E-X) | ||
+ | </td> | ||
+ | <td> | ||
+ | 17/09/2010 | ||
</td><td> | </td><td> | ||
- | <partinfo> | + | ''E. coli'' DH5-alpha |
+ | </td> | ||
+ | <td> | ||
+ | <partinfo>pSB1AK3</partinfo> | ||
+ | </td> | ||
+ | <td> | ||
+ | Length ok. | ||
+ | </td> | ||
+ | <td> | ||
+ | 2531 bp | ||
+ | </td> | ||
+ | <td>Pha10S + PhaSS + INTEIN + GFPs + TT</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">I54</font> | ||
+ | <br/> | ||
+ | (it was I54-a) | ||
+ | <br/> | ||
+ | </td> | ||
+ | <td> | ||
+ | I36 (E-S)<br/>+<br/>I37 (E-X) | ||
+ | </td> | ||
+ | <td> | ||
+ | 17/09/2010 | ||
</td><td> | </td><td> | ||
- | + | ''E. coli'' DH5-alpha | |
+ | </td> | ||
+ | <td> | ||
+ | <partinfo>pSB1AK3</partinfo> | ||
+ | </td> | ||
+ | <td> | ||
+ | Length ok. | ||
</td> | </td> | ||
- | <td></td><td> | + | <td> |
+ | 2531 bp | ||
+ | </td> | ||
+ | <td>PhaSS + PhaSS + INTEIN + GFPs + TT</td> | ||
</tr> | </tr> | ||
+ | |||
<tr> | <tr> | ||
<td> | <td> | ||
- | + | <font class="BBok">I55</font> | |
+ | <br/> | ||
+ | (it was I55-1) | ||
+ | <br/><partinfo>BBa_K300082</partinfo> | ||
+ | <br/> | ||
+ | </td> | ||
+ | <td> | ||
+ | I38 (E-S)<br/>+<br/>I20 (E-X) | ||
+ | </td> | ||
+ | <td> | ||
+ | 02/10/2010 | ||
</td><td> | </td><td> | ||
- | + | ''E. coli'' DH5-alpha | |
+ | </td> | ||
<td> | <td> | ||
- | + | <partinfo>pSB1AK3</partinfo> | |
+ | </td> | ||
+ | <td> | ||
+ | Seq ok (BMR), length ok. | ||
+ | </td> | ||
+ | <td> | ||
+ | 1663 bp | ||
+ | </td> | ||
+ | <td>HSL inducible system + Pha10S</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">I56</font> | ||
+ | <br/> | ||
+ | (it was I56-a) | ||
+ | <br/> | ||
+ | </td> | ||
+ | <td> | ||
+ | I26 (E-S)<br/>+<br/>I41 (E-X) | ||
+ | </td> | ||
+ | <td> | ||
+ | 17/09/2010 | ||
</td><td> | </td><td> | ||
''E. coli'' DH5-alpha | ''E. coli'' DH5-alpha | ||
+ | </td> | ||
+ | <td> | ||
+ | <partinfo>pSB1AK3</partinfo> | ||
+ | </td> | ||
+ | <td> | ||
+ | Length ok. | ||
+ | </td> | ||
+ | <td> | ||
+ | 2090 bp | ||
+ | </td> | ||
+ | <td>linker + PhaSS + linker + PhaSS + GFPs + TT</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">I57</font> | ||
+ | <br/> | ||
+ | (it was I57-a) | ||
+ | <br/> | ||
+ | </td> | ||
+ | <td> | ||
+ | I40 (E-S)<br/>+<br/>I0 (E-X) | ||
+ | </td> | ||
+ | <td> | ||
+ | 17/09/2010 | ||
</td><td> | </td><td> | ||
- | <partinfo> | + | ''E. coli'' DH5-alpha |
+ | </td> | ||
+ | <td> | ||
+ | <partinfo>pSB1AK3</partinfo> | ||
+ | </td> | ||
+ | <td> | ||
+ | Length ok. | ||
+ | </td> | ||
+ | <td> | ||
+ | 1985 bp | ||
+ | </td> | ||
+ | <td>linker + PhaSS + INTEIN + GFPs + TT</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">I58</font> | ||
+ | <br/> | ||
+ | (it was I58-c) | ||
+ | <br/> | ||
+ | </td> | ||
+ | <td> | ||
+ | I38 (E-S)<br/>+<br/>I31 (E-X) | ||
+ | </td> | ||
+ | <td> | ||
+ | 17/09/2010 | ||
</td><td> | </td><td> | ||
- | + | ''E. coli'' DH5-alpha | |
+ | </td> | ||
+ | <td> | ||
+ | <partinfo>pSB1A2</partinfo> | ||
+ | </td> | ||
+ | <td> | ||
+ | Length ok. | ||
</td> | </td> | ||
- | <td></td><td> | + | <td> |
+ | 1699 bp | ||
+ | </td> | ||
+ | <td>HSL inducible system + Pha10S + linker</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td> | <td> | ||
- | + | <font class="BBok">I59</font> | |
+ | <br/> | ||
+ | (it was I59-b) | ||
+ | <br/> | ||
+ | </td> | ||
+ | <td> | ||
+ | I21 (E-S)<br/> + <br/>I34 (E-X) | ||
+ | </td> | ||
+ | <td> | ||
+ | 17/09/2010 | ||
</td><td> | </td><td> | ||
- | + | ''E. coli'' DH5-alpha | |
+ | </td> | ||
<td> | <td> | ||
- | + | <partinfo>pSB1AK3</partinfo> | |
+ | </td> | ||
+ | <td> | ||
+ | Length ok. | ||
+ | </td> | ||
+ | <td> | ||
+ | 2018 bp | ||
+ | </td> | ||
+ | <td>PhaSS + PhaSS + GFPs + TT</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">I60</font> | ||
+ | <br/> | ||
+ | (it was I60-a) | ||
+ | <br/> | ||
+ | </td> | ||
+ | <td> | ||
+ | I20 (S-P)<br/>+<br/>I26 (X-P) | ||
+ | </td> | ||
+ | <td> | ||
+ | 17/09/2010 | ||
</td><td> | </td><td> | ||
''E. coli'' DH5-alpha | ''E. coli'' DH5-alpha | ||
+ | </td> | ||
+ | <td> | ||
+ | <partinfo>pSB1AK3</partinfo> | ||
+ | </td> | ||
+ | <td> | ||
+ | Seq ok (BMR), length ok. | ||
+ | </td> | ||
+ | <td> | ||
+ | 1194 bp | ||
+ | </td> | ||
+ | <td>Pha10S + linker + PhaSS</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">I61</font> | ||
+ | <br/> | ||
+ | (it was I61-a) | ||
+ | <br/> | ||
+ | </td> | ||
+ | <td> | ||
+ | I31 (E-S)<br/>+<br/>I37 (E-X) | ||
+ | </td> | ||
+ | <td> | ||
+ | 17/09/2010 | ||
</td><td> | </td><td> | ||
- | <partinfo> | + | ''E. coli'' DH5-alpha |
+ | </td> | ||
+ | <td> | ||
+ | <partinfo>pSB1AK3</partinfo> | ||
+ | </td> | ||
+ | <td> | ||
+ | Length ok. | ||
+ | </td> | ||
+ | <td> | ||
+ | 1985 bp | ||
+ | </td> | ||
+ | <td>Pha10S + linker + INTEIN + GFPs + TT</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">I62</font> | ||
+ | <br/> | ||
+ | (it was I62-E) | ||
+ | <br/> | ||
+ | </td> | ||
+ | <td> | ||
+ | I39 (S-P)<br/>+<br/>I59 (X-P) | ||
+ | </td> | ||
+ | <td> | ||
+ | 27/09/2010 | ||
</td><td> | </td><td> | ||
- | + | ''E. coli'' TOP10 | |
+ | </td> | ||
+ | <td> | ||
+ | <partinfo>pSB1A2</partinfo> | ||
+ | </td> | ||
+ | <td> | ||
+ | Length ok. | ||
</td> | </td> | ||
- | <td></td><td> | + | <td> |
+ | 2678 bp | ||
+ | </td> | ||
+ | <td>pTet + Pha10S + PhaSS + PhaSS + GFPs + TT</td> | ||
</tr> | </tr> | ||
+ | |||
<tr> | <tr> | ||
<td> | <td> | ||
- | + | <font class="BBok">I63</font> | |
+ | <br/> | ||
+ | (it was I63-1) | ||
+ | <br/><partinfo>BBa_K300091</partinfo> | ||
+ | <br/> | ||
+ | </td> | ||
+ | <td> | ||
+ | I38 (E-S)<br/>+<br/>I61 (E-X) | ||
+ | </td> | ||
+ | <td> | ||
+ | 24/09/2010 | ||
</td><td> | </td><td> | ||
- | + | ''E. coli'' TOP10 | |
+ | </td> | ||
<td> | <td> | ||
- | + | <partinfo>pSB1AK3</partinfo> | |
+ | </td> | ||
+ | <td> | ||
+ | Length ok. | ||
+ | </td> | ||
+ | <td> | ||
+ | 3072 bp | ||
+ | </td> | ||
+ | <td>HSL inducible system + Pha10S + linker + INTEIN + GFPs + TT</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">I64</font> | ||
+ | <br/> | ||
+ | (it was I64-2) | ||
+ | <br/><partinfo>BBa_K300092</partinfo> | ||
+ | <br/> | ||
+ | </td> | ||
+ | <td> | ||
+ | I58 (E-S)<br/>+<br/>I52 (E-X) | ||
+ | </td> | ||
+ | <td> | ||
+ | 24/09/2010 | ||
+ | </td><td> | ||
+ | ''E. coli'' TOP10 | ||
+ | </td> | ||
+ | <td> | ||
+ | <partinfo>pSB1A2</partinfo> | ||
+ | </td> | ||
+ | <td> | ||
+ | Length ok. | ||
+ | </td> | ||
+ | <td> | ||
+ | 3690 bp | ||
+ | </td> | ||
+ | <td>HSL inducible system + Pha10S + linker + PhaSS + linker + INTEIN + GFPs + TT</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">I65</font> | ||
+ | <br/> | ||
+ | (it was I65-3) | ||
+ | <br/><partinfo>BBa_K300099</partinfo> | ||
+ | <br/> | ||
+ | </td> | ||
+ | <td> | ||
+ | I39 (S-P)<br/>+<br/>I56 (X-P) | ||
+ | </td> | ||
+ | <td> | ||
+ | 24/09/2010 | ||
+ | </td><td> | ||
+ | ''E. coli'' TOP10 | ||
+ | </td> | ||
+ | <td> | ||
+ | <partinfo>pSB1A2</partinfo> | ||
+ | </td> | ||
+ | <td> | ||
+ | Length ok. | ||
+ | </td> | ||
+ | <td> | ||
+ | 2752 bp | ||
+ | </td> | ||
+ | <td>pTet + Pha10S + linker + PhaSS + linker + PhaSS + GFPs + TT</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">I66</font> | ||
+ | <br/> | ||
+ | (it was I66-1) | ||
+ | <br/> | ||
+ | </td> | ||
+ | <td> | ||
+ | I60 (E-S)<br/>+<br/>I57 (E-X) | ||
+ | </td> | ||
+ | <td> | ||
+ | 24/09/2010 | ||
+ | </td><td> | ||
+ | ''E. coli'' TOP10 | ||
+ | </td> | ||
+ | <td> | ||
+ | <partinfo>pSB1AK3</partinfo> | ||
+ | </td> | ||
+ | <td> | ||
+ | Length ok. | ||
+ | </td> | ||
+ | <td> | ||
+ | 3185 bp | ||
+ | </td> | ||
+ | <td>Pha10S + linker + PhaSS + linker + PhaSS + INTEIN + GFPs + TT</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">I70</font> | ||
+ | <br/> | ||
+ | (it was I70-1) | ||
+ | <br/><partinfo>BBa_K300007</partinfo> | ||
+ | <br/> | ||
+ | </td> | ||
+ | <td> | ||
+ | MyYeast3 (E-S)<br/>+<br/>I4 (E-S) | ||
+ | </td> | ||
+ | <td> | ||
+ | 01/10/10 | ||
+ | </td><td> | ||
+ | ''E. coli'' TOP10 | ||
+ | </td> | ||
+ | <td> | ||
+ | MyYeast (Amp100 in coli) | ||
+ | </td> | ||
+ | <td> | ||
+ | |||
+ | </td> | ||
+ | <td> | ||
+ | 5187bp | ||
+ | </td> | ||
+ | <td>MyYeast vector + I4 in cloning site (Ptef1 + mOrange + Tadh1)</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">I71</font> | ||
+ | <br/> | ||
+ | (it was I71-1) | ||
+ | </td> | ||
+ | <td> | ||
+ | MyYeast3 (E-S)<br/>+<br/>I1 (E-S) | ||
+ | </td> | ||
+ | <td> | ||
+ | 01/10/10 | ||
+ | </td><td> | ||
+ | ''E. coli'' TOP10 | ||
+ | </td> | ||
+ | <td> | ||
+ | MyYeast (Amp100 in coli) | ||
+ | </td> | ||
+ | <td> | ||
+ | |||
+ | </td> | ||
+ | <td> | ||
+ | 4778bp | ||
+ | </td> | ||
+ | <td>MyYeast vector + I1 in cloning site (mOrange + Tadh1, NO promoter)</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | I72<br/> | ||
+ | (it was I70-x) | ||
+ | </td> | ||
+ | <td> | ||
+ | <partinfo>BBa_J04450</partinfo>(E-P)+<partinfo>pSB4C5</partinfo>(E-P)</td> | ||
+ | <td> | ||
+ | |||
+ | </td><td> | ||
+ | ''E. coli'' TOP10 | ||
+ | </td> | ||
+ | <td> | ||
+ | <partinfo>pSB4C5</partinfo> | ||
+ | </td> | ||
+ | <td> | ||
+ | |||
+ | </td> | ||
+ | <td> | ||
+ | 4290bp | ||
+ | </td> | ||
+ | <td>mRFP under pLac promoter in LC plasmid pSB4C5</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">I73</font> | ||
+ | <br/> | ||
+ | (it was I73-1) | ||
+ | <br/> | ||
+ | </td> | ||
+ | <td> | ||
+ | I38 (E-S)<br/>+<br/>I53 (E-X) | ||
+ | </td> | ||
+ | <td> | ||
+ | 02/10/2010 | ||
+ | </td><td> | ||
+ | ''E. coli'' TOP10 | ||
+ | </td> | ||
+ | <td> | ||
+ | <partinfo>pSB1AK3</partinfo> | ||
+ | </td> | ||
+ | <td> | ||
+ | Length ok. | ||
+ | </td> | ||
+ | <td> | ||
+ | 3618 bp | ||
+ | </td> | ||
+ | <td>HSL inducible system + Pha10S + PhaSS + INTEIN + GFPs + TT</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">I74</font> | ||
+ | <br/> | ||
+ | (it was I74-8) | ||
+ | <br/> | ||
+ | </td> | ||
+ | <td> | ||
+ | I31 (E-S)<br/>+<br/>I35 (E-X) | ||
+ | </td> | ||
+ | <td> | ||
+ | 04/10/2010 | ||
+ | </td><td> | ||
+ | ''E. coli'' TOP10 | ||
+ | </td> | ||
+ | <td> | ||
+ | <partinfo>pSB1A2</partinfo> | ||
+ | </td> | ||
+ | <td> | ||
+ | Length ok. | ||
+ | </td> | ||
+ | <td> | ||
+ | 1230 bp | ||
+ | </td> | ||
+ | <td>Pha10S + linker + PhaSS + linker</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | I75<br/> | ||
+ | (it was I75-x) | ||
+ | </td> | ||
+ | <td> | ||
+ | I55 (E-S)<br/>+<br/>I37 (E-X) | ||
+ | </td> | ||
+ | <td> | ||
+ | Not screened yet. | ||
+ | </td><td> | ||
+ | ''E. coli'' TOP10 | ||
+ | </td> | ||
+ | <td> | ||
+ | <partinfo>pSB1AK3</partinfo> | ||
+ | </td> | ||
+ | <td> | ||
+ | Not screened yet. | ||
+ | </td> | ||
+ | <td> | ||
+ | 3036 bp | ||
+ | </td> | ||
+ | <td>HSL inducible system + Pha10S + INTEIN + GFPs + TT</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | I76<br/> | ||
+ | (it was I76-x) | ||
+ | </td> | ||
+ | <td> | ||
+ | I55 (E-S)<br/>+<br/>I54 (E-X) | ||
+ | </td> | ||
+ | <td> | ||
+ | Not screened yet. | ||
</td><td> | </td><td> | ||
''E. coli'' DH5-alpha | ''E. coli'' DH5-alpha | ||
+ | </td> | ||
+ | <td> | ||
+ | <partinfo>pSB1AK3</partinfo> | ||
+ | </td> | ||
+ | <td> | ||
+ | Not screened yet. | ||
+ | </td> | ||
+ | <td> | ||
+ | 4191 bp | ||
+ | </td> | ||
+ | <td>HSL inducible system + Pha10S + PhaSS + PhaSS + INTEIN + GFPs + TT</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | I77<br/> | ||
+ | (it was I77-x) | ||
+ | </td> | ||
+ | <td> | ||
+ | I55 (E-S)<br/>+<br/>I57 (E-X) | ||
+ | </td> | ||
+ | <td> | ||
+ | Not screened yet. | ||
</td><td> | </td><td> | ||
- | <partinfo> | + | ''E. coli'' TOP10 |
+ | </td> | ||
+ | <td> | ||
+ | <partinfo>pSB1AK3</partinfo> | ||
+ | </td> | ||
+ | <td> | ||
+ | Not screened yet. | ||
+ | </td> | ||
+ | <td> | ||
+ | 3654 bp | ||
+ | </td> | ||
+ | <td>HSL inducible system + Pha10S + linker + PhaSS + INTEIN + GFPs + TT</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">I78</font> | ||
+ | <br/> | ||
+ | (it was I78-3) | ||
+ | <br/><partinfo>BBa_K300084</partinfo> | ||
+ | <br/> | ||
+ | </td> | ||
+ | <td> | ||
+ | I74 (E-S)<br/>+<br/>INTEIN_C3 (E-X) | ||
+ | </td> | ||
+ | <td> | ||
+ | 07/10/10 | ||
</td><td> | </td><td> | ||
- | + | ''E. coli'' TOP10 | |
+ | </td> | ||
+ | <td> | ||
+ | <partinfo>pSB1C3</partinfo> | ||
+ | </td> | ||
+ | <td> | ||
+ | Seq ok (BMR), length ok. | ||
</td> | </td> | ||
- | <td></td><td> | + | <td> |
+ | 1743 bp | ||
+ | </td> | ||
+ | <td>Pha10S + linker + PhaSS + linker + INTEIN</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td> | <td> | ||
- | + | <font class="BBok">I79</font> | |
+ | <br/> | ||
+ | (it was I79-2) | ||
+ | <br/><partinfo>BBa_K300083</partinfo> | ||
+ | <br/> | ||
+ | </td> | ||
+ | <td> | ||
+ | I60 (E-S)<br/>+<br/>INTEIN_C3 (E-X) | ||
+ | </td> | ||
+ | <td> | ||
+ | 07/10/10 | ||
</td><td> | </td><td> | ||
- | + | ''E. coli'' STBL3 | |
+ | </td> | ||
<td> | <td> | ||
- | + | <partinfo>pSB1C3</partinfo> | |
+ | </td> | ||
+ | <td> | ||
+ | Seq ok (BMR), length ok. | ||
+ | </td> | ||
+ | <td> | ||
+ | 1707 bp | ||
+ | </td> | ||
+ | <td>Pha10S + linker + PhaSS + INTEIN</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">I80</font> | ||
+ | <br/> | ||
+ | (it was I80-4) | ||
+ | <br/><partinfo>BBa_K300010</partinfo> | ||
+ | <br/> | ||
+ | </td> | ||
+ | <td> | ||
+ | I3_1C3 (S-P)<br/>+<br/><partinfo>BBa_F2620</partinfo> (X-P) | ||
+ | </td> | ||
+ | <td> | ||
+ | 11/10/10 | ||
+ | </td><td> | ||
+ | ''E. coli'' TOP10 | ||
+ | </td> | ||
+ | <td> | ||
+ | <partinfo>pSB1C3</partinfo> | ||
+ | </td> | ||
+ | <td> | ||
+ | Seq ok (BMR), length ok. | ||
+ | </td> | ||
+ | <td> | ||
+ | 1707 bp | ||
+ | </td> | ||
+ | <td> | ||
+ | RBS + luxI + TT + <partinfo>BBa_F2620</partinfo> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">INTEIN</font> | ||
+ | <br/><partinfo>BBa_K300004</partinfo> | ||
+ | </td> | ||
+ | <td> | ||
+ | Intein | ||
+ | </td> | ||
+ | <td> | ||
+ | 13/08/2010 | ||
+ | </td><td> | ||
+ | ''E. coli'' TOP10 | ||
+ | </td> | ||
+ | <td> | ||
+ | pMA <br/> (HC Amp resistance) | ||
+ | </td> | ||
+ | <td> | ||
+ | Mr.Gene DNA synthesis | ||
+ | </td> | ||
+ | <td> | ||
+ | 507 bp | ||
+ | </td> | ||
+ | <td>INTEIN</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">INTEIN_C3</font> | ||
+ | </td> | ||
+ | <td> | ||
+ | Intein | ||
+ | </td> | ||
+ | <td> | ||
+ | 02/10/2010 | ||
+ | </td> | ||
+ | <td> | ||
+ | ''E. coli'' TOP10 | ||
+ | </td> | ||
+ | <td> | ||
+ | <partinfo>pSB1C3</partinfo> | ||
+ | </td> | ||
+ | <td> | ||
+ | Mr.Gene DNA synthesis | ||
+ | </td> | ||
+ | <td> | ||
+ | 507 bp | ||
+ | </td> | ||
+ | <td> | ||
+ | INTEIN in <partinfo>pSB1C3</partinfo> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">YEAST<br/>MR.G</font> | ||
+ | <br/><partinfo>BBa_K300980</partinfo> | ||
+ | </td> | ||
+ | <td> | ||
+ | YEAST fragment | ||
+ | </td> | ||
+ | <td> | ||
+ | 15/09/2010 | ||
</td><td> | </td><td> | ||
''E. coli'' DH5-alpha | ''E. coli'' DH5-alpha | ||
+ | </td> | ||
+ | <td> | ||
+ | pMA <br/> (HC Amp resistance) | ||
+ | </td> | ||
+ | <td> | ||
+ | Mr.Gene DNA synthesis | ||
+ | </td> | ||
+ | <td> | ||
+ | 1695 bp | ||
+ | </td> | ||
+ | <td>YEAST fragment</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">CRIM<br/>MR.G</font> | ||
+ | <br/><partinfo>BBa_K300983</partinfo> | ||
+ | </td> | ||
+ | <td> | ||
+ | CREAM fragment | ||
+ | </td> | ||
+ | <td> | ||
+ | 13/08/2010 | ||
</td><td> | </td><td> | ||
- | <partinfo> | + | ''E. coli'' TOP10 |
+ | </td> | ||
+ | <td> | ||
+ | pMK-RQ <br/> (HC Kan resistance) | ||
+ | </td> | ||
+ | <td> | ||
+ | Mr.Gene DNA synthesis | ||
+ | </td> | ||
+ | <td> | ||
+ | 829 bp | ||
+ | </td> | ||
+ | <td>CREAM fragment</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">CREAM</font> | ||
+ | <br/> | ||
+ | (it was MyCRIM) | ||
+ | <br/><partinfo>BBa_K300982</partinfo> | ||
+ | <br/> | ||
+ | </td> | ||
+ | <td> | ||
+ | CRIM<br/>MR.G (A-A) <br/>+<br/>I5 (S-X) | ||
+ | </td> | ||
+ | <td> | ||
+ | 21/08/2010 | ||
</td><td> | </td><td> | ||
- | + | ''E. coli'' BW23474 | |
+ | </td> | ||
+ | <td> | ||
+ | CREAM | ||
+ | </td> | ||
+ | <td> | ||
+ | Length ok. | ||
</td> | </td> | ||
- | <td></td><td> | + | <td> |
+ | 2149 bp | ||
+ | </td> | ||
+ | <td>CREAM fragment + Cm resistance + TT + R6K ori</td> | ||
</tr> | </tr> | ||
+ | |||
<tr> | <tr> | ||
<td> | <td> | ||
- | + | <font class="BBok">I7-4C5</font> | |
+ | <br/> | ||
+ | (it was I7-4C5-2) | ||
+ | </td> | ||
+ | <td> | ||
+ | I7 (E-P)<br/>+<br/> <partinfo>pSB4C5</partinfo>(E-P) | ||
+ | </td> | ||
+ | <td> | ||
+ | 16/07/2010 | ||
</td><td> | </td><td> | ||
- | <partinfo> | + | ''E. coli'' TOP10 |
+ | </td><td> | ||
+ | <partinfo>pSB4C5</partinfo> | ||
+ | </td><td> | ||
+ | Seq ok (BMR), length ok. | ||
+ | </td> | ||
+ | <td>2795 bp</td> | ||
+ | <td>constitutive promoter + RBS + luxI + TT + HSL sensor in <partinfo>pSB4C5</partinfo></td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | <tr> | ||
<td> | <td> | ||
- | 25/ | + | <font class="BBok">I8-4C5</font> |
+ | <br/> | ||
+ | (it was I8-4C5-2) | ||
+ | </td> | ||
+ | <td> | ||
+ | I8 (E-P)<br/>+<br/><partinfo>pSB4C5</partinfo> (E-P) | ||
+ | </td> | ||
+ | <td> | ||
+ | 16/07/2010 | ||
+ | </td><td> | ||
+ | ''E. coli'' TOP10 | ||
+ | </td><td> | ||
+ | <partinfo>pSB4C5</partinfo> | ||
+ | </td><td> | ||
+ | Seq ok (BMR), length ok. | ||
+ | </td> | ||
+ | <td>2795 bp</td> | ||
+ | <td>constitutive promoter + RBS + luxI + TT + HSL sensor in <partinfo>pSB4C5</partinfo></td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">I10-4C5</font> | ||
+ | <br/> | ||
+ | (it was I10-4C5-1) | ||
+ | </td> | ||
+ | <td> | ||
+ | I10 (E-P)<br/>+<br/><partinfo>pSB4C5</partinfo> (E-P) | ||
+ | </td> | ||
+ | <td> | ||
+ | 16/07/2010 | ||
+ | </td><td> | ||
+ | ''E. coli'' TOP10 | ||
+ | </td><td> | ||
+ | <partinfo>pSB4C5</partinfo> | ||
+ | </td><td> | ||
+ | Length ok. | ||
+ | </td> | ||
+ | <td> | ||
+ | 2795 bp | ||
+ | </td> | ||
+ | <td>constitutive promoter + RBS + luxI + TT + HSL sensor in <partinfo>pSB4C5</partinfo></td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">I12-4C5</font> | ||
+ | <br/> | ||
+ | (it was I12-4C5-1) | ||
+ | </td> | ||
+ | <td> | ||
+ | I12 (E-P)<br/>+<br/><partinfo>pSB4C5</partinfo> (E-P) | ||
+ | </td> | ||
+ | <td> | ||
+ | 25/07/2010 | ||
+ | </td><td> | ||
+ | ''E. coli'' TOP10 | ||
+ | </td><td> | ||
+ | <partinfo>pSB4C5</partinfo> | ||
+ | </td><td> | ||
+ | Length ok. | ||
+ | </td> | ||
+ | <td> | ||
+ | 2795 bp | ||
+ | </td> | ||
+ | <td>constitutive promoter + RBS + luxI + TT + HSL sensor in <partinfo>pSB4C5</partinfo></td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">I14-4C5</font> | ||
+ | <br/> | ||
+ | (it was I14-4C5-2) | ||
+ | </td> | ||
+ | <td> | ||
+ | I14 (E-P)<br/>+<br/><partinfo>pSB4C5</partinfo> (E-P) | ||
+ | </td> | ||
+ | <td> | ||
+ | 23/07/2010 | ||
</td><td> | </td><td> | ||
''E. coli'' DH5-alpha | ''E. coli'' DH5-alpha | ||
</td><td> | </td><td> | ||
- | <partinfo> | + | <partinfo>pSB4C5</partinfo> |
</td><td> | </td><td> | ||
- | + | Length ok. | |
</td> | </td> | ||
- | <td></td><td> | + | <td> |
+ | 842 bp | ||
+ | </td> | ||
+ | <td>constitutive promoter + RBS + luxI + TT + HSL sensor in <partinfo>pSB4C5</partinfo></td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td> | <td> | ||
- | + | <font class="BBok">I15-4C5</font> | |
+ | <br/> | ||
+ | (it was I15-4C5-2) | ||
+ | </td> | ||
+ | <td> | ||
+ | I15 (E-P)<br/>+<br/><partinfo>pSB4C5</partinfo> (E-P) | ||
+ | </td> | ||
+ | <td> | ||
+ | 13/08/2010 | ||
</td><td> | </td><td> | ||
- | <partinfo> | + | ''E. coli'' TOP10 |
+ | </td><td> | ||
+ | <partinfo>pSB4C5</partinfo> | ||
+ | </td><td> | ||
+ | Length ok. | ||
+ | </td> | ||
<td> | <td> | ||
- | + | 842 bp | |
+ | </td> | ||
+ | <td>constitutive promoter + RBS + luxI + TT in <partinfo>pSB4C5</partinfo></td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">I16-4C5</font> | ||
+ | <br/> | ||
+ | (it was I16-4C5-1) | ||
+ | </td> | ||
+ | <td> | ||
+ | I16 (E-P)<br/>+<br/><partinfo>pSB4C5</partinfo> (E-P) | ||
+ | </td> | ||
+ | <td> | ||
+ | 23/07/2010 | ||
</td><td> | </td><td> | ||
''E. coli'' DH5-alpha | ''E. coli'' DH5-alpha | ||
</td><td> | </td><td> | ||
- | <partinfo> | + | <partinfo>pSB4C5</partinfo> |
</td><td> | </td><td> | ||
- | + | Length ok. | |
</td> | </td> | ||
- | <td></td><td> | + | <td> |
+ | 842 bp | ||
+ | </td> | ||
+ | <td>constitutive promoter + RBS + luxI + TT in <partinfo>pSB4C5</partinfo></td> | ||
</tr> | </tr> | ||
+ | |||
+ | |||
+ | |||
<tr> | <tr> | ||
<td> | <td> | ||
- | + | <font class="BBok">I17-4C5</font> | |
+ | <br/> | ||
+ | (it was I17-4C5-1) | ||
+ | </td> | ||
+ | <td> | ||
+ | I17 (E-P)<br/>+<br/><partinfo>pSB4C5</partinfo> (E-P) | ||
+ | </td> | ||
+ | <td> | ||
+ | 25/07/2010 | ||
</td><td> | </td><td> | ||
- | <partinfo> | + | ''E. coli'' DH5-alpha |
+ | </td><td> | ||
+ | <partinfo>pSB4C5</partinfo> | ||
+ | </td><td> | ||
+ | Length ok. | ||
+ | </td> | ||
<td> | <td> | ||
- | 25/ | + | 842 bp |
+ | </td> | ||
+ | <td>constitutive promoter + RBS + luxI + TT in <partinfo>pSB4C5</partinfo></td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">I18-4C5</font> | ||
+ | <br/> | ||
+ | (it was I18-4C5-1) | ||
+ | </td> | ||
+ | <td> | ||
+ | I18 (E-P)<br/>+<br/><partinfo>pSB4C5</partinfo> (E-P) | ||
+ | </td> | ||
+ | <td> | ||
+ | 25/07/2010 | ||
</td><td> | </td><td> | ||
''E. coli'' DH5-alpha | ''E. coli'' DH5-alpha | ||
</td><td> | </td><td> | ||
- | <partinfo> | + | <partinfo>pSB4C5</partinfo> |
</td><td> | </td><td> | ||
- | + | Length ok. | |
</td> | </td> | ||
- | <td></td><td> | + | <td> |
+ | 842 bp | ||
+ | </td> | ||
+ | <td>constitutive promoter + RBS + luxI + TT in <partinfo>pSB4C5</partinfo></td> | ||
</tr> | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">I19-4C5</font> | ||
+ | <br/> | ||
+ | (it was I19-4C5-1) | ||
+ | </td> | ||
+ | <td> | ||
+ | I19 (E-P)<br/>+<br/><partinfo>pSB4C5</partinfo> (E-P) | ||
+ | </td> | ||
+ | <td> | ||
+ | 25/07/2010 | ||
+ | </td><td> | ||
+ | ''E. coli'' DH5-alpha | ||
+ | </td><td> | ||
+ | <partinfo>pSB4C5</partinfo> | ||
+ | </td><td> | ||
+ | Length ok. | ||
+ | </td> | ||
+ | <td> | ||
+ | 842 bp | ||
+ | </td> | ||
+ | <td>constitutive promoter + RBS + luxI + TT in <partinfo>pSB4C5</partinfo></td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | I27 | ||
+ | <br/> | ||
+ | </td> | ||
+ | <td> | ||
+ | I23 (E-P)<br/>+<br/><partinfo>pSB4C5</partinfo> (E-P) | ||
+ | </td> | ||
+ | <td> | ||
+ | 17/08/2010 | ||
+ | </td><td> | ||
+ | ''E. coli'' TOP10 | ||
+ | </td><td> | ||
+ | <partinfo>pSB4C5</partinfo> | ||
+ | </td><td> | ||
+ | |||
+ | </td> | ||
+ | <td> | ||
+ | 725 bp | ||
+ | </td> | ||
+ | <td>plux + RBS + luxI + TT in <partinfo>pSB4C5</partinfo></td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | I28 | ||
+ | </td> | ||
+ | <td> | ||
+ | I24 (E-P)<br/>+<br/><partinfo>pSB4C5</partinfo> (E-P) | ||
+ | </td> | ||
+ | <td> | ||
+ | 17/08/2010 | ||
+ | </td><td> | ||
+ | ''E. coli'' TOP10 | ||
+ | </td><td> | ||
+ | <partinfo>pSB4C5</partinfo> | ||
+ | </td><td> | ||
+ | |||
+ | </td> | ||
+ | <td> | ||
+ | 886 bp | ||
+ | </td> | ||
+ | <td>plux + RBS + lasI + terminator in <partinfo>pSB4C5</partinfo></td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">BBa_J23100<br/>_4C5</font> | ||
+ | <br/> | ||
+ | (it was <partinfo>BBa_J23100</partinfo><br/>_4C5-1) | ||
+ | </td> | ||
+ | <td> | ||
+ | <partinfo>BBa_J23100</partinfo> <br/>+<br/> <partinfo>BBa_B0034</partinfo> <br/>+<br/> <partinfo>BBa_E1010</partinfo> <br/>+<br/> <partinfo>BBa_B0015</partinfo> in <partinfo>pSB4C5</partinfo>. | ||
+ | </td> | ||
+ | <td> | ||
+ | 27/08/2010 | ||
+ | </td><td> | ||
+ | ''E. coli'' TOP10 | ||
+ | </td><td> | ||
+ | <partinfo>pSB4C5</partinfo> | ||
+ | </td><td> | ||
+ | Seq ok (BMR), length ok. | ||
+ | </td> | ||
+ | <td> | ||
+ | 904 bp | ||
+ | </td> | ||
+ | <td>constitutive promoter + RBS + mRFP1 + TT in <partinfo>pSB4C5</partinfo></td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">BBa_J23101<br/>_4C5</font> | ||
+ | <br/> | ||
+ | (it was <partinfo>BBa_J23101</partinfo><br/>_4C5-1) | ||
+ | </td> | ||
+ | <td> | ||
+ | <partinfo>BBa_J23101</partinfo> <br/>+<br/> <partinfo>BBa_B0034</partinfo> <br/>+<br/> <partinfo>BBa_E1010</partinfo> <br/>+<br/> <partinfo>BBa_B0015</partinfo> in <partinfo>pSB4C5</partinfo>. | ||
+ | </td> | ||
+ | <td> | ||
+ | 27/08/2010 | ||
+ | </td><td> | ||
+ | ''E. coli'' TOP10 | ||
+ | </td><td> | ||
+ | <partinfo>pSB4C5</partinfo> | ||
+ | </td><td> | ||
+ | Seq ok (BMR), length ok. | ||
+ | </td> | ||
+ | <td> | ||
+ | 904 bp | ||
+ | </td> | ||
+ | <td>constitutive promoter + RBS + mRFP1 + TT in <partinfo>pSB4C5</partinfo></td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">BBa_J23105<br/>_4C5</font> | ||
+ | <br/> | ||
+ | (it was <partinfo>BBa_J23105</partinfo><br/>_4C5-1) | ||
+ | </td> | ||
+ | <td> | ||
+ | <partinfo>BBa_J23105</partinfo> <br/>+<br/> <partinfo>BBa_B0034</partinfo> <br/>+<br/> <partinfo>BBa_E1010</partinfo> <br/>+<br/> <partinfo>BBa_B0015</partinfo> in <partinfo>pSB4C5</partinfo>. | ||
+ | </td> | ||
+ | <td> | ||
+ | 27/08/2010 | ||
+ | </td><td> | ||
+ | ''E. coli'' TOP10 | ||
+ | </td><td> | ||
+ | <partinfo>pSB4C5</partinfo> | ||
+ | </td><td> | ||
+ | Length ok. | ||
+ | </td> | ||
+ | <td> | ||
+ | 904 bp | ||
+ | </td> | ||
+ | <td>constitutive promoter + RBS + mRFP1 + TT in <partinfo>pSB4C5</partinfo></td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">BBa_J23106<br/>_4C5</font> | ||
+ | <br/> | ||
+ | (it was <partinfo>BBa_J23106</partinfo><br/>_4C5-1) | ||
+ | </td> | ||
+ | <td> | ||
+ | <partinfo>BBa_J23106</partinfo> <br/>+<br/> <partinfo>BBa_B0034</partinfo> <br/>+<br/> <partinfo>BBa_E1010</partinfo> <br/>+<br/> <partinfo>BBa_B0015</partinfo> in <partinfo>pSB4C5</partinfo>. | ||
+ | </td> | ||
+ | <td> | ||
+ | 27/08/2010 | ||
+ | </td><td> | ||
+ | ''E. coli'' TOP10 | ||
+ | </td><td> | ||
+ | <partinfo>pSB4C5</partinfo> | ||
+ | </td><td> | ||
+ | Length ok. | ||
+ | </td> | ||
+ | <td> | ||
+ | 904 bp | ||
+ | </td> | ||
+ | <td>constitutive promoter + RBS + mRFP1 + TT in <partinfo>pSB4C5</partinfo></td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">BBa_J23110<br/>_4C5</font> | ||
+ | <br/> | ||
+ | (it was <partinfo>BBa_J23110</partinfo><br/>_4C5-1) | ||
+ | </td> | ||
+ | <td> | ||
+ | <partinfo>BBa_J23110</partinfo> <br/>+<br/> <partinfo>BBa_B0034</partinfo> <br/>+<br/> <partinfo>BBa_E1010</partinfo> <br/>+<br/> <partinfo>BBa_B0015</partinfo> in <partinfo>pSB4C5</partinfo>. | ||
+ | </td> | ||
+ | <td> | ||
+ | 27/08/2010 | ||
+ | </td><td> | ||
+ | ''E. coli'' TOP10 | ||
+ | </td><td> | ||
+ | <partinfo>pSB4C5</partinfo> | ||
+ | </td><td> | ||
+ | Length ok. | ||
+ | </td> | ||
+ | <td> | ||
+ | 904 bp | ||
+ | </td> | ||
+ | <td>constitutive promoter + RBS + mRFP1 + TT in <partinfo>pSB4C5</partinfo></td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">BBa_J23114<br/>_4C5</font> | ||
+ | <br/> | ||
+ | (it was <partinfo>BBa_J23114</partinfo><br/>_4C5-1) | ||
+ | </td> | ||
+ | <td> | ||
+ | <partinfo>BBa_J23114</partinfo> <br/>+<br/> <partinfo>BBa_B0034</partinfo> <br/>+<br/> <partinfo>BBa_E1010</partinfo> <br/>+<br/> <partinfo>BBa_B0015</partinfo> in <partinfo>pSB4C5</partinfo>. | ||
+ | </td> | ||
+ | <td> | ||
+ | 27/08/2010 | ||
+ | </td><td> | ||
+ | ''E. coli'' TOP10 | ||
+ | </td><td> | ||
+ | <partinfo>pSB4C5</partinfo> | ||
+ | </td><td> | ||
+ | Length ok. | ||
+ | </td> | ||
+ | <td> | ||
+ | 904 bp | ||
+ | </td> | ||
+ | <td>constitutive promoter + RBS + mRFP1 + TT in <partinfo>pSB4C5</partinfo></td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">BBa_J23116<br/>_4C5</font> | ||
+ | <br/> | ||
+ | (it was <partinfo>BBa_J23116</partinfo><br/>_4C5-1) | ||
+ | </td> | ||
+ | <td> | ||
+ | <partinfo>BBa_J23116</partinfo> <br/>+<br/> <partinfo>BBa_B0034</partinfo> <br/>+<br/> <partinfo>BBa_E1010</partinfo> <br/>+<br/> <partinfo>BBa_B0015</partinfo> in <partinfo>pSB4C5</partinfo>. | ||
+ | </td> | ||
+ | <td> | ||
+ | 30/08/2010 | ||
+ | </td><td> | ||
+ | ''E. coli'' TOP10 | ||
+ | </td><td> | ||
+ | <partinfo>pSB4C5</partinfo> | ||
+ | </td><td> | ||
+ | Length ok. | ||
+ | </td> | ||
+ | <td> | ||
+ | 904 bp | ||
+ | </td> | ||
+ | <td>constitutive promoter + RBS + mRFP1 + TT in <partinfo>pSB4C5</partinfo></td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">BBa_J23118<br/>_4C5</font> | ||
+ | <br/> | ||
+ | (it was <partinfo>BBa_J23118</partinfo><br/>_4C5-1) | ||
+ | </td> | ||
+ | <td> | ||
+ | <partinfo>BBa_J23118</partinfo> <br/>+<br/> <partinfo>BBa_B0034</partinfo> <br/>+<br/> <partinfo>BBa_E1010</partinfo> <br/>+<br/> <partinfo>BBa_B0015</partinfo> in <partinfo>pSB4C5</partinfo>. | ||
+ | </td> | ||
+ | <td> | ||
+ | 30/08/2010 | ||
+ | </td><td> | ||
+ | ''E. coli'' TOP10 | ||
+ | </td><td> | ||
+ | <partinfo>pSB4C5</partinfo> | ||
+ | </td><td> | ||
+ | Seq ok (BMR), length ok. | ||
+ | </td> | ||
+ | <td> | ||
+ | 904 bp | ||
+ | </td> | ||
+ | <td>constitutive promoter + RBS + mRFP1 + TT in <partinfo>pSB4C5</partinfo></td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | <tr><td colspan="8"> </td></tr> | ||
+ | <!-- Ceppi ordinati --> | ||
+ | <tr> | ||
+ | <th>Strain name</th> | ||
+ | <th>Description</th> | ||
+ | <th>Arrival date</th> | ||
+ | <th>Infos</th> | ||
+ | <th>Resistance</th> | ||
+ | <th>Quality control</th> | ||
+ | <th>Notes</th> | ||
+ | <th>Store</th> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | MG1655(Seq)<br/>(MG) | ||
+ | </td> | ||
+ | <td> | ||
+ | ''E. coli'' wild type strain fully sequenced | ||
+ | </td> | ||
+ | <td> | ||
+ | 28/06/2010 | ||
+ | </td> | ||
+ | <td> | ||
+ | <html><a href="http://cgsc.biology.yale.edu/Strain.php?ID=68076" target="_blank">link</a></html> | ||
+ | </td> | ||
+ | <td>-</td> | ||
+ | <td></td> | ||
+ | <td>Genome fully sequenced</td> | ||
+ | <td>Strains 2010</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | MC1061<br/>(MC) | ||
+ | </td> | ||
+ | <td> | ||
+ | ''E. coli'' strain | ||
+ | </td> | ||
+ | <td> | ||
+ | 28/06/2010 | ||
+ | </td> | ||
+ | <td> | ||
+ | <html><a href="http://cgsc.biology.yale.edu/Strain.php?ID=11225" target="_blank">link</a></html> | ||
+ | </td> | ||
+ | <td>-</td> | ||
+ | <td></td> | ||
+ | <td>Strain used by JC Anderson et al. for integration</td> | ||
+ | <td>Strains 2010</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | BW25141 <br/>(BW1) | ||
+ | </td> | ||
+ | <td> | ||
+ | ''E. coli'' pir+ strain | ||
+ | </td> | ||
+ | <td> | ||
+ | 28/06/2010 | ||
+ | </td> | ||
+ | <td> | ||
+ | <html><a href="http://cgsc.biology.yale.edu/Strain.php?ID=64690" target="_blank">link</a></html> | ||
+ | </td> | ||
+ | <td>-</td> | ||
+ | <td>Actually replicates the conditional replication origin R6K</td> | ||
+ | <td>LC pir</td> | ||
+ | <td>Strains 2010</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | BW25142 <br/>(BW2) | ||
+ | </td> | ||
+ | <td> | ||
+ | ''E. coli'' pir116 strain | ||
+ | </td> | ||
+ | <td> | ||
+ | 28/06/2010 | ||
+ | </td> | ||
+ | <td> | ||
+ | <html><a href="http://cgsc.biology.yale.edu/Strain.php?ID=70715" target="_blank">link</a></html> | ||
+ | </td> | ||
+ | <td>-</td> | ||
+ | <td>Actually replicates the conditional replication origin R6K</td> | ||
+ | <td>HC pir</td> | ||
+ | <td>Strains 2010</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | BW23473 | ||
+ | </td> | ||
+ | <td> | ||
+ | ''E. coli'' pir+ strain | ||
+ | </td> | ||
+ | <td> | ||
+ | 19/07/2010 | ||
+ | </td> | ||
+ | <td> | ||
+ | <html><a href="http://cgsc.biology.yale.edu/Strain.php?ID=70679" target="_blank">link</a></html> | ||
+ | </td> | ||
+ | <td>-</td> | ||
+ | <td></td> | ||
+ | <td>LC pir</td> | ||
+ | <td>Strains 2010</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | BW23474 | ||
+ | </td> | ||
+ | <td> | ||
+ | ''E. coli'' pir116 strain | ||
+ | </td> | ||
+ | <td> | ||
+ | 28/06/2010 | ||
+ | </td> | ||
+ | <td> | ||
+ | <html><a href="http://cgsc.biology.yale.edu/Strain.php?ID=70692" target="_blank">link</a></html> | ||
+ | </td> | ||
+ | <td>-</td> | ||
+ | <td>Actually replicates the conditional replication origin R6K</td> | ||
+ | <td>HC pir</td> | ||
+ | <td>Strains 2010</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | BW5328/pAH123 | ||
+ | </td> | ||
+ | <td> | ||
+ | ''E. coli'' strain | ||
+ | </td> | ||
+ | <td> | ||
+ | 28/06/2010 | ||
+ | </td> | ||
+ | <td> | ||
+ | <html><a href="http://cgsc.biology.yale.edu/Strain.php?ID=70678" target="_blank">link</a></html> | ||
+ | </td> | ||
+ | <td>Amp 50 ug/ml</td> | ||
+ | <td>Plasmid length ok (after digestion with SpeI).</td> | ||
+ | <td> | ||
+ | Contains pAH123 HELPER plasmid carrying IntPhi80, low copy Amp, ori-ts 30°C | ||
+ | </td> | ||
+ | <td>Strains 2010</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | BT340 | ||
+ | </td> | ||
+ | <td> | ||
+ | ''E. coli'' strain | ||
+ | </td> | ||
+ | <td> | ||
+ | 28/06/2010 | ||
+ | </td> | ||
+ | <td> | ||
+ | <html><a href="http://cgsc.biology.yale.edu/Strain.php?ID=64645" target="_blank">link</a></html> | ||
+ | </td> | ||
+ | <td>Amp 100 ug/ml and Cm 34 ug/ml</td> | ||
+ | <td>Plasmid relative molecular weight ok (after gel run of the undigested plasmid)</td> | ||
+ | <td> | ||
+ | Contains pCP20 helper with yeast FRT recombinase, Amp, Cm, ori-ts 30°C | ||
+ | </td> | ||
+ | <td>Strains 2010</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | DSM15372 | ||
+ | </td> | ||
+ | <td> | ||
+ | ''E. coli'' DMS15372 strain | ||
+ | </td> | ||
+ | <td> | ||
+ | 08/07/2010 | ||
+ | </td> | ||
+ | <td> | ||
+ | <html><a href="http://www.dsmz.de/microorganisms/html/strains/strain.dsm015372.html" target="_blank">link</a></html> | ||
+ | </td> | ||
+ | <td>Amp 100 ug/ml</td> | ||
+ | <td>Length ok (after digestion with EcoRI)</td> | ||
+ | <td> | ||
+ | Harbours the pBHR68 plasmid (contains an IPTG-inducible phaCAB operon) | ||
+ | </td> | ||
+ | <td>Strains 2010</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | </table> | ||
+ | |||
+ | </td> | ||
+ | </tr> | ||
</table> | </table> |
Latest revision as of 14:30, 27 October 2010
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