Team:Northwestern/Project/Lac

From 2010.igem.org

(Difference between revisions)
 
(4 intermediate revisions not shown)
Line 110: Line 110:
<!--- The Mission, Experiments --->
<!--- The Mission, Experiments --->
-
{|- style="font-size: 87%; background: #AFC7C7; color: #000000" cellpadding="1" cellspacing="1" bordercolor="#000000" width="75%" align="center"
+
{|- style="font-size: 87%; background: #EFCDF8; color: #FFFFFF" cellpadding="1" cellspacing="1" bordercolor="#000000" width="75%" align="center"
-
!align="center"|[[Team:Northwestern|<font color="#FFFFFF">'''Home'''</font>]]
+
!align="center"|[[Team:Northwestern|<font color="#2B3856">'''Home'''</font>]]
-
!align="center"|[[Team:Northwestern/Brainstorm|<font color="#FFFFFF">'''Brainstorm'''</font>]]
+
!align="center"|[[Team:Northwestern/Brainstorm|<font color="#000000">'''Brainstorm'''</font>]]
-
!align="center"|[[Team:Northwestern/Team|<font color="#FFFFFF">'''Team'''</font>]]
+
!align="center"|[[Team:Northwestern/Team|<font color="#000000">'''Team'''</font>]]
-
!align="center"|[[Team:Northwestern/Acknowledgements|<font color="#FFFFFF">'''Acknowledgements'''</font>]]
+
!align="center"|[[Team:Northwestern/Acknowledgements|<font color="#000000">'''Acknowledgements'''</font>]]
-
!align="center"|[[Team:Northwestern/Project|<font color="#2B3856">'''Project'''</font>]]
+
!align="center"|[[Team:Northwestern/Project|<font color="#000000">'''Project'''</font>]]
-
!align="center"|[[Team:Northwestern/SideProject|<font color="#FFFFFF">'''Human Practices'''</font>]]
+
!align="center"|[[Team:Northwestern/SideProject|<font color="#000000">'''Human Practices'''</font>]]
-
!align="center"|[[Team:Northwestern/Parts|<font color="#FFFFFF">'''Parts'''</font>]]
+
!align="center"|[[Team:Northwestern/Parts|<font color="#000000">'''Parts'''</font>]]
-
!align="center"|[[Team:Northwestern/Notebook|<font color="#FFFFFF">'''Notebook'''</font>]]
+
!align="center"|[[Team:Northwestern/Notebook|<font color="#000000">'''Notebook'''</font>]]
-
!align="center"|[[Team:Northwestern/Calendar|<font color="#FFFFFF">'''Calendar'''</font>]]
+
!align="center"|[[Team:Northwestern/Calendar|<font color="#000000">'''Calendar'''</font>]]
-
!align="center"|[[Team:Northwestern/Protocol|<font color="#FFFFFF">'''Protocol'''</font>]]
+
!align="center"|[[Team:Northwestern/Protocol|<font color="#000000">'''Protocol'''</font>]]
-
!align="center"|[[Team:Northwestern/Safety|<font color="#FFFFFF">'''Safety'''</font>]]
+
!align="center"|[[Team:Northwestern/Safety|<font color="#000000">'''Safety'''</font>]]
-
!align="center"|[[Team:Northwestern/Links|<font color="#FFFFFF">'''Links'''</font>]]
+
!align="center"|[[Team:Northwestern/Links|<font color="#000000">'''Links'''</font>]]
-
!align="center"|[[Team:Northwestern/References|<font color="#FFFFFF">'''References'''</font>]]
+
!align="center"|[[Team:Northwestern/References|<font color="#000000">'''References'''</font>]]
-
!align="center"|[[Team:Northwestern/Media|<font color="#FFFFFF">'''Media'''</font>]]
+
!align="center"|[[Team:Northwestern/Media|<font color="#000000">'''Media'''</font>]]
-
!align="center"|[[Team:Northwestern/Contact|<font color="#FFFFFF">'''Contact'''</font>]]
+
!align="center"|[[Team:Northwestern/Contact|<font color="#000000">'''Contact'''</font>]]
|}
|}
{| align="center" border="0" width="75%"
{| align="center" border="0" width="75%"
-
|align="left" valign="top" width="80%" style="padding: 5"|
+
|colspan="2"|
-
For the induction system, our team decided to use the lac-operon/iptg construct because of its widespread use and the availability of information.
+
<html>
 +
<table align="center">
 +
<tr>
 +
<td><a href="https://2010.igem.org/Team:Northwestern/Project/Modeling"><img width="100" class="icon" src="https://static.igem.org/mediawiki/2010/a/a7/NU_modeling.jpg"></a></td>
 +
<td><a href="https://2010.igem.org/Team:Northwestern/Project/Chassis"><img width="100" class="icon" src="https://static.igem.org/mediawiki/2010/a/a6/Nuchassis.jpg"></a></td>
 +
<td><a href="https://2010.igem.org/Team:Northwestern/Project/Lac"><img width="100" class="icon" src="https://static.igem.org/mediawiki/2010/1/18/Nulac.jpg"></a></td>
 +
<td><a href="https://2010.igem.org/Team:Northwestern/Project/Chitin Synthesis"><img width="100" class="icon" src="https://static.igem.org/mediawiki/2010/4/4e/Nuchitin.jpg"></a></td>
 +
<td><a href="https://2010.igem.org/Team:Northwestern/Project/Apoptosis"><img width="100" class="icon" src="https://static.igem.org/mediawiki/2010/c/cb/Nuapop.jpg"></a></td>
 +
</tr>
 +
<tr>
 +
<td align="center">Modeling</td>
 +
<td align="center">Chassis</td>
 +
<td align="center">Induction</td>
 +
<td align="center">Chitin</td>
 +
<td align="center">Apoptosis</td>
 +
</tr>
 +
</table>
 +
</html>
 +
 
 +
|-
 +
|align="left" valign="top" width="80%" style="padding: 5"|
 +
=='''Induction'''==
-
The induction construct consisted of a constitutive promoter (J23100, J23104, or J23105), a combination part of Ribosome Binding Site, Lac Repressor, double Terminators, and Lac Promoter (Q01121, Q04121), and Ribosome Binding Sequence (B0034, B0031, B0032).
+
===Induction System===
 +
For the induction system, our team decided to use the lac-operon/iptg construct because of its widespread use and the availability of information. This system was assembled using standard biobricks parts. To induce our system, we used a IPTG spray of two and five millimolar concentrations, and one millimolar solutions for liquid cultures.
-
Multiple versions of each part were used to generate a wide range of repressor concentrations, so as to allow for variation between the induction time-delay and degree of Apoptosis protein and Chitin Synthase synthesis.
+
===Parts and Assembly===
 +
The induction construct consisted of a constitutive promoter (J23100, J23104, or J23105), a combination part of Ribosome Binding Site, Lac Repressor, double Terminators, and Lac Promoter (Q01121, Q04121), and Ribosome Binding Sequence (B0034, B0031, B0032). Various combinations of these parts were assembled to create varying induction speeds for Chitin Synthase and apoptosis. See the parts section for more information.
-
The effectiveness of these constructs were tested in the modeling section.
+
===Diffusion and Modeling===
 +
To induce our system, IPTG is sprayed onto the biofilm, diffusing through the top layer of the bacteria. This diffusion through the bacterial membrane is detailed in the modeling section.  
|}
|}

Latest revision as of 18:57, 23 October 2010


Tech Institute
Home Brainstorm Team Acknowledgements Project Human Practices Parts Notebook Calendar Protocol Safety Links References Media Contact

Modeling Chassis Induction Chitin Apoptosis

Induction

Induction System

For the induction system, our team decided to use the lac-operon/iptg construct because of its widespread use and the availability of information. This system was assembled using standard biobricks parts. To induce our system, we used a IPTG spray of two and five millimolar concentrations, and one millimolar solutions for liquid cultures.

Parts and Assembly

The induction construct consisted of a constitutive promoter (J23100, J23104, or J23105), a combination part of Ribosome Binding Site, Lac Repressor, double Terminators, and Lac Promoter (Q01121, Q04121), and Ribosome Binding Sequence (B0034, B0031, B0032). Various combinations of these parts were assembled to create varying induction speeds for Chitin Synthase and apoptosis. See the parts section for more information.

Diffusion and Modeling

To induce our system, IPTG is sprayed onto the biofilm, diffusing through the top layer of the bacteria. This diffusion through the bacterial membrane is detailed in the modeling section.

Retrieved from "http://2010.igem.org/Team:Northwestern/Project/Lac"