Team:Harvard/color/inhibition

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<h1>engineering metabolic pathways</h1>
<h1>engineering metabolic pathways</h1>
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<p>Our goal is to accumulate the pigments lycopene and beta-carotene in the petals of arabidopsis, and we plan to do so by inhibiting the enzymes lycopene epsilon cyclase (LUT2), carotene beta-ring hydroxylase (BETA-OHASE 1), and lycopene beta cyclase (LYC) via artificial microRNA interference.</p>
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<p>Our goal is to accumulate the pigments lycopene and beta-carotene in the petals of <i>Arabidopsis</i>, and we plan to do so by inhibiting the enzymes lycopene epsilon cyclase (LUT2), carotene beta-ring hydroxylase (BETA-OHASE 1), and lycopene beta cyclase (LYC) via <a href="https://2010.igem.org/Team:Harvard/allergy/methods">artificial microRNA interference</a>. Ordinarily, these pigments are intermediates in the carotenoid biosynthesis pathway, and we hope that by interfering with the pathway, we can cause an abnormal accumulation of these intermediates and thus produce color.</p>
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Latest revision as of 00:33, 27 October 2010



engineering metabolic pathways

Our goal is to accumulate the pigments lycopene and beta-carotene in the petals of Arabidopsis, and we plan to do so by inhibiting the enzymes lycopene epsilon cyclase (LUT2), carotene beta-ring hydroxylase (BETA-OHASE 1), and lycopene beta cyclase (LYC) via artificial microRNA interference. Ordinarily, these pigments are intermediates in the carotenoid biosynthesis pathway, and we hope that by interfering with the pathway, we can cause an abnormal accumulation of these intermediates and thus produce color.

In our first scheme, we will knock out LUT2 on its own. We hope that this will induce accumulation of lycopene.



In our second scheme, we will knock out BETA-OHASE 1 on its own. We hope that this will induce accumulation of beta-carotene.



In our third scheme, we will knock out both LUT2 and BETA-OHASE1. We hope that this will induce accumulation of some lycopene and some beta-carotene.


In our fourth scheme, we will knock out both LUT2 and LYC. We hope that this will induce accumulation of lycopene.



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