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UTDallas/21 October 2010
From 2010.igem.org
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===October 21, 2010 === | ===October 21, 2010 === | ||
*Made carb. plates | *Made carb. plates | ||
| - | *The transformations of the ligated parts worked. | + | *The transformations of the ligated parts worked. Everything but the negative controls had colonies. |
*Transformed the ligated PalkB+GFP | *Transformed the ligated PalkB+GFP | ||
*Performed titration of PyeaR+RFP with 0M, 10uM, 500uM, 10mM, and 100mM potassium nitrate, aluminum nitrate nonahydrate, and potassium nitrite | *Performed titration of PyeaR+RFP with 0M, 10uM, 500uM, 10mM, and 100mM potassium nitrate, aluminum nitrate nonahydrate, and potassium nitrite | ||
| + | ;Alkane sensor | ||
| + | *-ve control had colonies in it which is not a good sign. | ||
| + | *However still screened 26 colonied from the other plates and two from the -ve ctrl to see if in case anything is positive. | ||
Latest revision as of 20:26, 27 October 2010
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October 21, 2010
- Made carb. plates
- The transformations of the ligated parts worked. Everything but the negative controls had colonies.
- Transformed the ligated PalkB+GFP
- Performed titration of PyeaR+RFP with 0M, 10uM, 500uM, 10mM, and 100mM potassium nitrate, aluminum nitrate nonahydrate, and potassium nitrite
- Alkane sensor
- -ve control had colonies in it which is not a good sign.
- However still screened 26 colonied from the other plates and two from the -ve ctrl to see if in case anything is positive.
