Team:UNIPV-Pavia/Material Methods/Measurements/Tecan/test1ottobre

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Latest revision as of 21:31, 25 October 2010

OCTOBER, 1ST

PLATE

EXPERIMENT DESCRIPTION

Motivation

The experiment was performed to evaluate the initiation transcription point (in term of absorbance) for self-inducible devices.

Methods

The experiment was performed in M9 according to Protocol 1 on the following measurement parts:

<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300024</partinfo> (wiki name: I7)
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300021</partinfo> (wiki name: I8)
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300022</partinfo> (wiki name: I9)
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300023</partinfo> (wiki name: I10)
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300019</partinfo> (wiki name: I12)
<partinfo>pSB1A2</partinfo> - <partinfo>K173001</partinfo> (positive control)
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_B0032</partinfo> (negative control)
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300030</partinfo> (wiki name I14) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300028</partinfo> (wiki name I15) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300029</partinfo> (wiki name I16) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300025</partinfo> (wiki name I17) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300026</partinfo> (wiki name I18) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300027</partinfo> (wiki name I19) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
<partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo> (negative control)
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300024</partinfo> (wiki name: I7-4C5)
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300021</partinfo> (wiki name: I8-4C5)
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300023</partinfo> (wiki name: I10-4C5)
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300019</partinfo> (wiki name: I12-4C5)

RESULTS

Data were analyzed following the data analysis procedure to evaluate the ODstart. The plots correpsoning to the parts tested are reported:

The ODstart are reported in the table below:

Part	ODstart
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300024</partinfo>	0.0260
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300021</partinfo>	0.1024
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300022</partinfo>	0.3933
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300023</partinfo>	0.3466
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300019</partinfo>	0.3213
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300030</partinfo>	0.0522
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300028</partinfo>	0.1655
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300029</partinfo>	-
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300025</partinfo>	0.1058
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300026</partinfo>	0.5087
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300027</partinfo>	0.3559
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300024</partinfo>	0.2407
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300021</partinfo>	-
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300023</partinfo>	-
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300019</partinfo>	-

Tecan Test

@@ Line 11: / Line 11: @@
 <td valign="top">
 <table border="0" align="center" width="100%"><tr><td align="justify" valign="top" style="padding:20px">
-<html><p align="center"><font size="5"><b>25° TEST</b></font><br><br><font size="4">[OCTOBER, 1ST]</font></p></html><hr><br>
+<html><p align="center"><font size="5"><b>OCTOBER, 1ST</b></font></p></html><hr><br>
 ==PLATE==
 <br>
@@ Line 28: / Line 28: @@
 * <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300022</partinfo> (wiki name: I9)
 * <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300023</partinfo> (wiki name: I10)
-* <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300012</partinfo> (wiki name: I12)
+* <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300019</partinfo> (wiki name: I12)
 * <partinfo>pSB1A2</partinfo> - <partinfo>K173001</partinfo> (positive control)
 * <partinfo>pSB1A2</partinfo> - <partinfo>BBa_B0032</partinfo> (negative control)
@@ Line 41: / Line 41: @@
 * <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300021</partinfo> (wiki name: I8-4C5)
 * <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300023</partinfo> (wiki name: I10-4C5)
-* <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300012</partinfo> (wiki name: I12-4C5)
+* <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300019</partinfo> (wiki name: I12-4C5)
-==RESULTS (?)==
+==RESULTS==
+Data were analyzed following the [[Team:UNIPV-Pavia/Parts/Characterization#Data analysis for self-inducible promoters (initiation-treshold determination)| data analysis procedure]] to evaluate the ODstart. The plots correpsoning to the parts tested are reported:
+[[Image:UNIPV_Pavia_FluoVSODHC0110.png| 620px | center ]]
+[[Image:UNIPV_Pavia_FluoVSODCo0110.png| 620px | center ]]
+[[Image:UNIPV_Pavia_FluoVSODLC0110.png| 620px | center ]]
+The ODstart are reported in the table below:
+{| border='1' align='center'
+|| Part || ODstart
+|-
+|| <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300024</partinfo> || 0.0260
+|-
+|| <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300021</partinfo> || 0.1024
+|-
+|| <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300022</partinfo> || 0.3933
+|-
+|| <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300023</partinfo> || 0.3466
+|-
+|| <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300019</partinfo> || 0.3213
+|-
+|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300030</partinfo> || 0.0522
+|-
+|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300028</partinfo> || 0.1655
+|-
+|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300029</partinfo> || -
+|-
+|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300025</partinfo> || 0.1058
+|-
+|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300026</partinfo> || 0.5087
+|-
+|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300027</partinfo> || 0.3559
+|-
+|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300024</partinfo> || 0.2407
+|-
+|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300021</partinfo> || -
+|-
+|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300023</partinfo> || -
+|-
+|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300019</partinfo> || -
+|}
 <!-- table previous next test -->
 <br><br>