Team:Newcastle/problem

From 2010.igem.org

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=Problem=
=Problem=
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==Concrete Background==
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==Bacilla Filla repairing cracks in concrete==
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[[Media:Concrete.pdf|Deena's concrete lecture]] provides some basic knowledge on concrete as well as answers to questions asked after our presentation at the [[UK_Meetup_2010#UK_iGEM_get-together.2C_Newcastle.2C__20th_and_21st_July.2C_2010|2010 iGEM UK Get Together]].
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===Concrete Background===
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[[Media:Concrete.pdf|Deena's concrete lecture]] provides some basic knowledge on concrete as well as questions from our presentation at iGEM UK Get Together.
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===Reasons cracks are bad===   
===Reasons cracks are bad===   
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{|
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|[[Image:newcastle_corrosion.jpg|400px]]
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|[[Image:newcastle_brokenbridge.jpg|400px]]
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|}
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# Allow water to reach and corrode steel reinforcements  
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# Cracks allow water to reach and corrode steel reinforcements;
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# Weaken concrete structures
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# They weaken concrete structures;
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# Difficult to repair
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# They are difficult to repair.
Work in this area has already been started:
Work in this area has already been started:
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[http://www.youtube.com/watch?v=PyBR3PDPa-c Bio-concrete] and a non-biological method of [http://ace-mrl.engin.umich.edu/| self healing concrete]
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[http://www.youtube.com/watch?v=PyBR3PDPa-c Bio-concrete] and a non-biological method [http://ace-mrl.engin.umich.edu/ self healing concrete].
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Those projects mentioned above are different from ours because they only allow bacteria to repair cracks that form almost instantly after concrete sets, which are cracks formed in new buildings, whereas our Bacilla Filla can repair cracks that were formed in existing structures. This makes a lot of difference to the construction industry because it can halt the process of corrosion in steel reinforcements in existing structures. Also, our cells would die after a certain period of time after repairing the crack thus making Bacilla Filla environmentally friendly.
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The projects mentioned above are different from our project because they only use bacteria to repair cracks that form in specialised concrete in new buildings, whereas our BacillaFilla can repair cracks that were formed in existing structures. This makes a big difference to the construction industry because it can protect against the process of corrosion in steel reinforcements in existing structures.
===How our project is helpful===
===How our project is helpful===
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# Reduces corrosion rate of the steel reinforcements;
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Our project aims to:
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# Reduces the need to demolish and replace existing concrete structures;
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# Reduces cement production;
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# Reduce the rate of corrosion of the steel reinforcements
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# Reduces carbon dioxide emission from cement production;
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# Reduce the need to demolish and replace existing concrete structures
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# Reduces consumption of energy resources.
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# Reduce the need for cement production
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# Reduce carbon dioxide emission from cement production
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# Reduce the consumption of energy resources
===How our project is novel===  
===How our project is novel===  
# Our project focuses not only on newly set concrete structures, but also existing ones.
# Our project focuses not only on newly set concrete structures, but also existing ones.
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# All the cells will produce calcium carbonate which has similar properties to concrete
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# All the cells will produce calcium carbonate which has similar properties as that of concrete.
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# Filamentous ''Bacillus subtilis'' cells have similar properties to the synthetic fibres used in fibre-reinforced concrete, and thereby act as reinforcements within the crack.
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# Filamentous ''Bacillus subtilis'' cells have similar properties to the synthetic fibres used in fibre-reinforced concrete, and thereby act as reinforcements within the cracks.
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# Getting the BacillaFilla to produce glue which will hold everything together and will fill up the crack  
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# Getting BacillaFilla to produce levans glue that is able to hold the calcium carbonate and filamentous cells together. It will then fill up the crack.
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# Environmental kill switch
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# Environmental kill switch which allows BacillaFilla to kill itself in the absence of sucrose, which we will add to the cracks in the concrete.
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# We are working with a well known strain: '''''Bacillus subtilis''''' '''168''' (previous work required specific stains)
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# We are working with a well known strain: ''Bacillus subtilis'' 168 (previous work required specific strains).
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===5th August 2010===
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===Visit to the Structures Lab===
====Concrete Splitting Test====
====Concrete Splitting Test====
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On the 5th of August, the whole team went down to the '''Structures Lab''' of Cassie Building that houses the School of Civil Engineering and Geosciences, to do tensile splitting test on a concrete cylinder. We tried to understand the problems of crack formation on the concrete structures and how our project BacillaFilla will help.
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On the 5th of August, the whole team went down to the Structures Lab of Cassie Building that houses the School of Civil Engineering and Geosciences, to do tensile splitting test on a concrete cylinder. We tried to understand the problems of crack formation on the concrete structures and how our project BacillaFilla can help.
====More photos====
====More photos====
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{|
{|
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|[[Image:Newcastle_Concrete_1.jpg|thumb|Dr. Colin Davie showing properties of concrete]]
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|[[Image:Newcastle_Concrete_1.jpg|thumb|Dr. Colin Davie showing steel reinforcements of concrete]]
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|[[Image:Newcastle_Concrete_5.jpg|thumb|How tension form cracks within the concrete]]
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|[[Image:Newcastle_Concrete_5.jpg|thumb|How cracks form in bridges]]
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|[[Image:Newcastle_Concrete_2.jpg|thumb|Concrete in compression testing machine]]
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|[[Image:Newcastle_Concrete_2.jpg|thumb|Concrete in tensile strength testing machine]]
|[[Image:Newcastle_Concrete_17.jpg|thumb|Concrete cylinder cracked into two]]
|[[Image:Newcastle_Concrete_17.jpg|thumb|Concrete cylinder cracked into two]]
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|[[Image:Newcastle_Concrete_14.jpg|thumb|Teaching properties of concrete]]
|[[Image:Newcastle_Concrete_14.jpg|thumb|Teaching properties of concrete]]
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|[[Image:Newcastle_Concrete_15.jpg|thumb|Compression testing machine]]
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|[[Image:Newcastle_Concrete_15.jpg|thumb|Tensile strength testing machine]]
|[[Image:Newcastle_Concrete_16.jpg|thumb|Dr. Colin Davie carrying cracked concrete]]
|[[Image:Newcastle_Concrete_16.jpg|thumb|Dr. Colin Davie carrying cracked concrete]]
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|[[Image:Newcastle_Concrete_3.jpg|thumb|Concrete in compression testing machine]]
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|[[Image:Newcastle_Concrete_3.jpg|thumb|Concrete in tensile strength testing machine]]
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|[[Image:Newcastle_Concrete_4.jpg|thumb|right|Lab assistant showing how concrete cracked]]
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|[[Image:Newcastle_Concrete_4.jpg|thumb|right|Structures lab assistant, Billie explaining how concrete cracked]]
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===References===
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#Neville A. (2008) ''Properties of Concrete''. 4th Edition, London, Pearson Education Limited.
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{{Team:Newcastle/footer}}
{{Team:Newcastle/footer}}

Latest revision as of 17:43, 26 October 2010

iGEM Homepage Newcastle University BacillaFilla Homepage Image Map

Contents

Problem

Concrete Background

Deena's concrete lecture provides some basic knowledge on concrete as well as answers to questions asked after our presentation at the 2010 iGEM UK Get Together.

Reasons cracks are bad

Newcastle corrosion.jpg Newcastle brokenbridge.jpg
  1. Cracks allow water to reach and corrode steel reinforcements;
  2. They weaken concrete structures;
  3. They are difficult to repair.

Work in this area has already been started: [http://www.youtube.com/watch?v=PyBR3PDPa-c Bio-concrete] and a non-biological method [http://ace-mrl.engin.umich.edu/ self healing concrete].

The projects mentioned above are different from our project because they only use bacteria to repair cracks that form in specialised concrete in new buildings, whereas our BacillaFilla can repair cracks that were formed in existing structures. This makes a big difference to the construction industry because it can protect against the process of corrosion in steel reinforcements in existing structures.

How our project is helpful

Our project aims to:

  1. Reduce the rate of corrosion of the steel reinforcements
  2. Reduce the need to demolish and replace existing concrete structures
  3. Reduce the need for cement production
  4. Reduce carbon dioxide emission from cement production
  5. Reduce the consumption of energy resources

How our project is novel

  1. Our project focuses not only on newly set concrete structures, but also existing ones.
  2. All the cells will produce calcium carbonate which has similar properties as that of concrete.
  3. Filamentous Bacillus subtilis cells have similar properties to the synthetic fibres used in fibre-reinforced concrete, and thereby act as reinforcements within the cracks.
  4. Getting BacillaFilla to produce levans glue that is able to hold the calcium carbonate and filamentous cells together. It will then fill up the crack.
  5. Environmental kill switch which allows BacillaFilla to kill itself in the absence of sucrose, which we will add to the cracks in the concrete.
  6. We are working with a well known strain: Bacillus subtilis 168 (previous work required specific strains).

Visit to the Structures Lab

Concrete Splitting Test

On the 5th of August, the whole team went down to the Structures Lab of Cassie Building that houses the School of Civil Engineering and Geosciences, to do tensile splitting test on a concrete cylinder. We tried to understand the problems of crack formation on the concrete structures and how our project BacillaFilla can help.

More photos

Dr. Colin Davie showing steel reinforcements of concrete
How cracks form in bridges
Concrete in tensile strength testing machine
Concrete cylinder cracked into two
Teaching properties of concrete
Tensile strength testing machine
Dr. Colin Davie carrying cracked concrete
Concrete in tensile strength testing machine
Structures lab assistant, Billie explaining how concrete cracked

Materials used to mix concrete

Coarse aggregates
Fine aggregates
Cement

References

  1. Neville A. (2008) Properties of Concrete. 4th Edition, London, Pearson Education Limited.


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