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UTDallas/20 October 2010
From 2010.igem.org
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(New page: *Transformed the ligated PyeaR parts *Miniprepped, digested, ran a gel electrophoresis (took only the bottom band (~3000bp), gel purified, dephosphorylated, and then PCR purified GFP *Liga...) |
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| + | {{Template:UTDallasTop}} | ||
| + | {{Template:UTDallasMenu}} | ||
| + | <span style="color:#EDE8E8"> | ||
| + | <html> | ||
| + | <style type="text/css"> | ||
| + | table, tr, td {background:transparent; border:0px;} | ||
| + | table table table {background-color:COLOR;} */to change background color from white to transparent | ||
| + | |||
| + | table.calendar { margin: 0; padding: 0px; background-color:#660099} | ||
| + | table.calendar td { margin: 0; padding: 0px; vertical-align: top;} | ||
| + | |||
| + | table.month { margin: 0; padding: 0px; background-color:#E7E5E5} | ||
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| + | table.month .heading td { padding:0px; background-color:#660099; color: white; text-align:center; font-size:100%; font-weight:bold; } | ||
| + | table.month .dow td { color:#660099; text-align:center; font-size:90%; } | ||
| + | table.month td.today { background-color:##007D47; } | ||
| + | table.month td { | ||
| + | border: none; | ||
| + | margin: 0; | ||
| + | padding: 0pt 0pt; | ||
| + | font-weight: bold; | ||
| + | font-size: 8 pt; | ||
| + | text-align: center; | ||
| + | background-color: #E7E5E5; | ||
| + | } | ||
| + | #bodyContent table.month a { background:#none; padding:0 } | ||
| + | .day-active { color:#FF8000} | ||
| + | .day-empty { color:#000000} | ||
| + | </style> | ||
| + | </html> | ||
| + | |||
| + | ==Notebook== | ||
| + | |||
| + | {| align="center" | ||
| + | |-align="top" | ||
| + | |align="center" width="150pt"|{{#calendar: title=UTDallas |year=2010 | month=06}} | ||
| + | |align="center" width="150pt"|{{#calendar: title=UTDallas |year=2010 | month=07}} | ||
| + | |align="center" width="150pt"|{{#calendar: title=UTDallas |year=2010 | month=08}} | ||
| + | |align="center" width="150pt"|{{#calendar: title=UTDallas |year=2010 | month=09}} | ||
| + | |align="center" width="150pt"|{{#calendar: title=UTDallas |year=2010 | month=10}} | ||
| + | |align="center" width="150pt"|{{#calendar: title=UTDallas |year=2010 | month=11}} | ||
| + | |} | ||
| + | |||
| + | |||
| + | |||
| + | ===October 20, 2010 === | ||
| + | ;Nitrate Sensor | ||
*Transformed the ligated PyeaR parts | *Transformed the ligated PyeaR parts | ||
*Miniprepped, digested, ran a gel electrophoresis (took only the bottom band (~3000bp), gel purified, dephosphorylated, and then PCR purified GFP | *Miniprepped, digested, ran a gel electrophoresis (took only the bottom band (~3000bp), gel purified, dephosphorylated, and then PCR purified GFP | ||
*Ligated GFP to PalkB (the alkane promoter) | *Ligated GFP to PalkB (the alkane promoter) | ||
*Incubated PyeaR+RFP overnight | *Incubated PyeaR+RFP overnight | ||
| + | ;Alkane Sensor | ||
| + | * Midi prep part E0240 (vector) was cut with the restriction enzyme XbaI and incubated at 37C for 1 Hour | ||
| + | * Heat kill the enzyme after digestion (65C 10min) | ||
| + | * Dephosphorylation of the digested vector using CIP | ||
| + | * Neucloetide removal after Dephosphorylation. | ||
| + | * Already had phosphorylated insert i.e the Palk Promoter. | ||
| + | * Set up overnight ligation at 12C. | ||
Latest revision as of 20:12, 27 October 2010
Notebook
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October 20, 2010
- Nitrate Sensor
- Transformed the ligated PyeaR parts
- Miniprepped, digested, ran a gel electrophoresis (took only the bottom band (~3000bp), gel purified, dephosphorylated, and then PCR purified GFP
- Ligated GFP to PalkB (the alkane promoter)
- Incubated PyeaR+RFP overnight
- Alkane Sensor
- Midi prep part E0240 (vector) was cut with the restriction enzyme XbaI and incubated at 37C for 1 Hour
- Heat kill the enzyme after digestion (65C 10min)
- Dephosphorylation of the digested vector using CIP
- Neucloetide removal after Dephosphorylation.
- Already had phosphorylated insert i.e the Palk Promoter.
- Set up overnight ligation at 12C.
