Latest revision as of 03:12, 28 October 2010

Promoters

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DREB1C promoter Team:Nevada/registry submissions

The DREB1C iGEM promoter is derived from a transcription factor that is up-regulated by cold stress and down-regulated by circadian controls to prevent plant growth retardation due to the buildup of DREB1C (Dehydration Response Element Binding Protein) during the day (the cause of dwarfism). The promoter region for DREB1C begins just upstream of the translational start sequence (ATG) and contains 463 bp of the upstream sequence with six ADA independent, cis-acting elements for the up-regulation during cold stress and circadian controlled down-regulation.

The DREB1C iGEM promoter was isolated using custom primers directly from the Arabidopsis thaliana genome via PCR, blunt end Topo cloned, and then ligated to pSB1C3 using EcoR1 and Pst1 sites.

References
Kazuo Nakashima and Kazuko Yamaguchi-Shinozakia, Regulons involved in osmotic stress-responsive and cold stress-responsive gene expression in plants, Physiologia Plantarum 126: 62–71. 2006 Satoshi Kidokoro, Kyonoshin Maruyama, Kazuo Nakashima, Yoshiyuki Imura2, Yoshihiro Narusaka3, Zabta K. Shinwari4, Yuriko Osakabe, Yasunari Fujita, Junya Mizoi, Kazuo Shinozaki, and Kazuko Yamaguchi-Shinozaki, The Phytochrome-Interacting Factor PIF7 Negatively Regulates DREB1 Expression under Circadian Control in Arabidopsis, Plant Physiol. Vol. 151, 2009

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 == Promoters ==
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 <li><a href="https://2010.igem.org/Team:Nevada/RD29A" tabindex="2">rd29A</a></li>
 <li><a href="https://2010.igem.org/Team:Nevada/35S" tabindex="3">35S</a></li>
+<li><a href="https://2010.igem.org/Team:Nevada/CD2Inducible" tabindex="4">CD2+ Inducible</a></li>
 </ul>
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+https://static.igem.org/mediawiki/2010/9/98/Finished_final.png '''DREB1C promoter''' [[Team:Nevada/registry submissions]]
+----
-<p><html>
+<p><html><a href="https://static.igem.org/mediawiki/igem.org/d/d1/Chris_Igem.jpg"><img src="https://static.igem.org/mediawiki/igem.org/d/d1/Chris_Igem.jpg" class="shadow" style="float:left;width:200px;margin:10px"></a>
-<a href="https://static.igem.org/mediawiki/igem.org/d/d1/Chris_Igem.jpg"><img src="https://static.igem.org/mediawiki/igem.org/d/d1/Chris_Igem.jpg" class="shadow" style="float:left;width:200px;margin:10px"></a>
+</html> The <html><a href="https://2010.igem.org/Team:Nevada/DREB1CPromoter"><span style="color:#1569C7;font-weight:bold;">DREB1C</span></a></html> iGEM promoter is derived from a transcription factor that is up-regulated by cold stress and down-regulated by circadian controls to prevent plant growth retardation due to the buildup of <html><a href="https://2010.igem.org/Team:Nevada/DREB1CPromoter"><span style="color:#1569C7;font-weight:bold;">DREB1C</span></a></html> (Dehydration Response Element Binding Protein) during the day (the cause of dwarfism). The promoter region for <html><a href="https://2010.igem.org/Team:Nevada/DREB1CPromoter"><span style="color:#1569C7;font-weight:bold;">DREB1C</span></a></html> begins just upstream of the translational start sequence (ATG) and contains 463 bp of the upstream sequence with six ADA independent, <i>cis</i>-acting elements for the up-regulation during cold stress and circadian controlled down-regulation.</p>
-</html> The '''DREB1C iGEM promoter''' is derived from a transcription factor that is up-regulated by cold stress and down-regulated by circadian controls to prevent plant growth retardation due to the buildup of DREB1C (Dehydration Response Element Binding Protein) during the day (the cause of dwarfism). The promoter region for DREB1C begins just upstream of the translational start sequence (ATG) and contains 441bp of the upstream sequence with six cis-acting, ADA independent elements for the up-regulation during cold stress and circadian down-regulation.</p>
-<br><p><b>Kazuo Nakashima and Kazuko Yamaguchi-Shinozakia</b>, Regulons involved in osmotic stress-responsive and cold stress-responsive gene expression in plants, Physiologia Plantarum 126: 62–71. 2006</p>
-<br><p><b>Satoshi Kidokoro, Kyonoshin Maruyama, Kazuo Nakashima, Yoshiyuki Imura2, Yoshihiro Narusaka3, Zabta K. Shinwari4, Yuriko Osakabe, Yasunari Fujita, Junya Mizoi, Kazuo Shinozaki, and Kazuko Yamaguchi-Shinozaki</b>, The Phytochrome-Interacting Factor PIF7 Negatively Regulates DREB1 Expression under Circadian Control in Arabidopsis, Plant Physiol. Vol. 151, 2009</p>
+<p>The <html><a href="https://2010.igem.org/Team:Nevada/DREB1CPromoter"><span style="color:#1569C7;font-weight:bold;">DREB1C</span></a></html> iGEM promoter was isolated using custom primers directly from the <i>Arabidopsis thaliana</i> genome via PCR, blunt end Topo cloned, and then ligated to pSB1C3 using EcoR1 and Pst1 sites.</p>
+<br>'''References'''<br>
-|
+'''Kazuo Nakashima and Kazuko Yamaguchi-Shinozakia''', Regulons involved in osmotic stress-responsive and cold stress-responsive gene expression in plants, Physiologia Plantarum 126: 62–71. 2006
-|}
+'''Satoshi Kidokoro, Kyonoshin Maruyama, Kazuo Nakashima, Yoshiyuki Imura2, Yoshihiro Narusaka3, Zabta K. Shinwari4, Yuriko Osakabe, Yasunari Fujita, Junya Mizoi, Kazuo Shinozaki, and Kazuko Yamaguchi-Shinozaki''', The Phytochrome-Interacting Factor PIF7 Negatively Regulates DREB1 Expression under Circadian Control in Arabidopsis, Plant Physiol. Vol. 151, 2009
-<!--- The Mission, Experiments --->
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-'''We would like to thank the following sponsors for their support in helping us make this project possible.'''
-Much thanks to the [http://www.cabnr.unr.edu/Students/Majors.aspx Departments of Biochemistry and Biotechnology] and the [http://www.cabnr.unr.edu/Students/Glance.aspx College of Agriculture, Biotechnology and Natural Resources] for their encouragement and support. Thank you [http://www.unr.edu/inbre/ Nevada INBRE] for over $6,000 in support for supplies and registration costs.
-Thank you to [https://asun.unr.edu/Default.aspx Associated Students of the Univeristy of Nevada] for supporting our fund raising efforts.
-Thank you to [http://www.promega.com/Catalog/CountrySelect.aspx?returnurl=/Default.asp Promega Co.] for free enzyme donations.
-Thank you to [http://www.invitrogen.com/site/us/en/home.html?cid=covinvggl89100000002336s& Invitrogen Co.] for a discount on our Vector NTI program.
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