Team:Northwestern/Notebook

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[[Image:LOGOBANNER1.jpg|1000px|center|link=http://wikipedia.org|Tech Institute]]
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[[Image:LOGOBANNER1.jpg|936px|center|link=http://wikipedia.org|Tech Institute]]
<!--- The Mission, Experiments --->
<!--- The Mission, Experiments --->
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{|- style="font-size: 87%; background: #EFCDF8; color: #FFFFFF" cellpadding="1" cellspacing="1" bordercolor="#000000" width="75%" align="center"
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!align="center"|[[Team:Northwestern|<span style="color:#000000;">'''Home'''</span>]]
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!align="center"|[[Team:Northwestern|<font color="#2B3856">'''Home'''</font>]]
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!align="center"|[[Team:Northwestern/Brainstorm|<span style="color:#000000;">'''Brainstorm'''</span>]]
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!align="center"|[[Team:Northwestern/Brainstorm|<font color="#000000">'''Brainstorm'''</font>]]
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!align="center"|[[Team:Northwestern/Team|<span style="color:#000000;">'''Team'''</span>]]
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!align="center"|[[Team:Northwestern/Team|<font color="#000000">'''Team'''</font>]]
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!align="center"|[[Team:Northwestern/Acknowledgements|<span style="color:#000000;">'''Acknowledgements'''</span>]]
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!align="center"|[[Team:Northwestern/Acknowledgements|<font color="#000000">'''Acknowledgements'''</font>]]
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!align="center"|[[Team:Northwestern/Project|<span style="color:#000000;">'''Project'''</span>]]
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!align="center"|[[Team:Northwestern/Project|<font color="#000000">'''Project'''</font>]]
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!align="center"|[[Team:Northwestern/SideProject|<span style="color:#000000;">'''Human Practices'''</span>]]
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!align="center"|[[Team:Northwestern/SideProject|<font color="#000000">'''Human Practices'''</font>]]
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!align="center"|[[Team:Northwestern/Parts|<span style="color:#000000;">'''Parts'''</span>]]
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!align="center"|[[Team:Northwestern/Parts|<font color="#000000">'''Parts'''</font>]]
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!align="center"|[[Team:Northwestern/Notebook|<span style="color:#000000;">'''Notebook'''</span>]]
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!align="center"|[[Team:Northwestern/Notebook|<font color="#000000">'''Notebook'''</font>]]
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!align="center"|[[Team:Northwestern/Calendar|<span style="color:#000000;">'''Calendar'''</span>]]
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!align="center"|[[Team:Northwestern/Calendar|<font color="#000000">'''Calendar'''</font>]]
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!align="center"|[[Team:Northwestern/Protocol|<span style="color:#000000;">'''Protocol'''</span>]]
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!align="center"|[[Team:Northwestern/Protocol|<font color="#000000">'''Protocol'''</font>]]
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!align="center"|[[Team:Northwestern/Safety|<span style="color:#000000;">'''Safety'''</span>]]
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!align="center"|[[Team:Northwestern/Safety|<font color="#000000">'''Safety'''</font>]]
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!align="center"|[[Team:Northwestern/Links|<span style="color:#000000;">'''Links'''</span>]]
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!align="center"|[[Team:Northwestern/Links|<font color="#000000">'''Links'''</font>]]
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!align="center"|[[Team:Northwestern/References|<span style="color:#000000;">'''References'''</span>]]
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!align="center"|[[Team:Northwestern/References|<font color="#000000">'''References'''</font>]]
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!align="center"|[[Team:Northwestern/Media|<span style="color:#000000;">'''Media'''</span>]]
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!align="center"|[[Team:Northwestern/Media|<font color="#000000">'''Media'''</font>]]
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!align="center"|[[Team:Northwestern/Contact|<span style="color:#000000;">'''Contact'''</span>]]
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!align="center"|[[Team:Northwestern/Contact|<font color="#000000">'''Contact'''</font>]]
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{| align="center" border="0" width="75%"
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='''Notebook'''=
[[Brainstorming April-June 2010]]
[[Brainstorming April-June 2010]]
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NOTE: All work done by the undergraduate students of NU iGEM.
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=='''June'''==
[[Week1 6/13/10-6/19/10]]
[[Week1 6/13/10-6/19/10]]
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[[Week3 6/27/10-7/3/10]]
[[Week3 6/27/10-7/3/10]]
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=='''July'''==
[[Week4 7/4/10-7/10/10]]
[[Week4 7/4/10-7/10/10]]
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[[Week7 7/25/10-7/31/10]]
[[Week7 7/25/10-7/31/10]]
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=='''August'''==
[[Week8 8/1/10-8/7/10]]
[[Week8 8/1/10-8/7/10]]
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[[Week12 8/29/10-9/4/10]]
[[Week12 8/29/10-9/4/10]]
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=='''September'''==
[[Week13 9/5/10-9/11/10]]
[[Week13 9/5/10-9/11/10]]
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[[Week16 9/26/10-10/2/10]]
[[Week16 9/26/10-10/2/10]]
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=='''October'''==
[[Week17 10/3/10-10/9/10]]
[[Week17 10/3/10-10/9/10]]
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=='''November'''==
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[[Jamboree at MIT 11/05/10-11/07/10 :: Team Perspectives]]
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===10/3/10===
 
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Timi miniprepped the CP-LacPI-GFP cultures and digested them with E/P. She then poured a big and a small gel.
 
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Kevin came in and ran the digested CP-LacPI-GFP, digested CP-LacPI with digested Cp and digested LacPI parts.
 
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===10/4/10===
 
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Timi and Kevin decided to take a day off :)
 
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===10/5/10===
 
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Timi and Kevin came in to discuss why their gels were running strangely. We believe there is something wrong with our buffer, so we are swapping everything out with a fresh batch of Prof. Mordacq's 0.5x TBE buffer. Timi poured a large gel that we will be using tomorrow.
 
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===10/6/10===
 
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===10/7/10===
 
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Timi- took part 2-17F out of the DNA kit. Transformed into T10 cells to grow overnight.
 
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===10/8/10===
 
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Kevin- ran gel of the CP-LacPI parts. Gel results are still inconclusive. Also put in an overnight culture of the 2-17F part into the incubator for Timi.
 
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===10/9/10===
 
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Timi- Miniprepped the 2-17F part. Digested the DNA with E/P and ligated the 2-17F GFP part into Chlor and Tet backbone.
 
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===10/10/10===
 
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Timi- Transformed and plated part 2-17F onto Chlor and Tet plates. Will be ready for miniprepping tomorrow.
 
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===10/11/10===
 
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===10/12/10===
 
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Matt - digested LacP-RBS with SpeI in preparation for standard assembly with ES digested CHS3
 
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===10/13/10===
 
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===10/14/10===
 
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Kevin transformed T10 cells with CP-LacPI parts that we did not have much digest of left. He plated them onto TET plates.
 
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===10/15/10===
 
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Kevin took out the CP-LacPI plates and put them into the 4 degree for Timi. Timi inoculated overnight cultures at 7pm.
 
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===10/16/10===
 
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Timi and Kevin miniprepped the CP-LacPI inoculations, digested them with E&P, and ligated them to GFP.
 
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===10/18/10===
 
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Matt miniprepped 31GFP, 21GFP (old), and 32GFP (old). He digested the miniprepped DNA with E/P for Kevin to run on a gel. He also reinoculated all 9 CP-LacPI combinations.
 
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Latest revision as of 05:54, 20 March 2011


Tech Institute
Home Brainstorm Team Acknowledgements Project Human Practices Parts Notebook Calendar Protocol Safety Links References Media Contact

Notebook

Brainstorming April-June 2010

NOTE: All work done by the undergraduate students of NU iGEM.

June

Week1 6/13/10-6/19/10

Week2 6/20/10-6/26/10

Week3 6/27/10-7/3/10


July

Week4 7/4/10-7/10/10

Week5 7/11/10-7/17/10

Week6 7/18/10-7/24/10

Week7 7/25/10-7/31/10


August

Week8 8/1/10-8/7/10

Week9 8/8/10-8/14/10

Week10 8/15/10-8/21/10

Week11 8/22/10-8/28/10

Week12 8/29/10-9/4/10


September

Week13 9/5/10-9/11/10

Week14 9/12/10-9/18/10

Week15 9/19/10-9/25/10

Week16 9/26/10-10/2/10


October

Week17 10/3/10-10/9/10

Week18 10/10/10-10/16/10

Week19 10/17/10-10/23/10

Week20 10/24/10-10/30/10


November

Jamboree at MIT 11/05/10-11/07/10 :: Team Perspectives

Retrieved from "http://2010.igem.org/Team:Northwestern/Notebook"