Team:Calgary/8 June 2010

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Tuesday June 8, 2010|
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<h1>University of Calgary</h1>
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'''Tuesday June 8, 2010'''
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[[Image:06.08.2010.AC_and_AK_Plasmid_backbones.png|350px|thumb|Dev, Jeremy, and Chris's spread plates of Ampicillin - Chloramphenicol and Ampicillin - Kanamycin from the Parts Registry.]]
[[Image:06.08.2010.AC_and_AK_Plasmid_backbones.png|350px|thumb|Dev, Jeremy, and Chris's spread plates of Ampicillin - Chloramphenicol and Ampicillin - Kanamycin from the Parts Registry.]]
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[[Image:06.08.2010.Amp_%2B_Kan_Plasmid_backbones.png|350px|thumb|Dev, Jeremy, and Chris's spread plates of the Ampicillin and Kanamycin plasmid backbones from the Parts Registry.]]
[[Image:06.08.2010.Amp_%2B_Kan_Plasmid_backbones.png|350px|thumb|Dev, Jeremy, and Chris's spread plates of the Ampicillin and Kanamycin plasmid backbones from the Parts Registry.]]
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[[Image:06.08.2010 R0040 E0430 Spread Plates.jpg|350px|thumb|Alex's and Patrick's spread plates. Photograph taken at 8:48am June 8th, 2010.]]
[[Image:06.08.2010 R0040 E0430 Spread Plates.jpg|350px|thumb|Alex's and Patrick's spread plates. Photograph taken at 8:48am June 8th, 2010.]]
Today, we spent most of the day making overnight cultures from the transformed plates that were made yesterday. As a group, we began getting fundraising underway by finding contacts, dividng them among each person and setting a tentative goal of $20 000 to fundraise from corporate sponsors. We also organized how the money given from Alberta Innovates would be used  
Today, we spent most of the day making overnight cultures from the transformed plates that were made yesterday. As a group, we began getting fundraising underway by finding contacts, dividng them among each person and setting a tentative goal of $20 000 to fundraise from corporate sponsors. We also organized how the money given from Alberta Innovates would be used  
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Chris, Jeremy, and Dev: Most of the growth on the plates were red, indicating that the placeholder gene was being transcribed and successfully produced. Our group spent today making overnight cultures so plasmid transfers could be done tomorrow. We made the cultures for plasmids pSB1A3, pSB1K2, pSB1AC3 and pSB1AK3 as well as restreaking the colonies that were used  to innoculate the overnight cultures. Jeremy also transformed I732005 (lacZ gene for B-galactosidase) into competent cells so they could be used.
 
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Alex and Patrick: The ampicillin spread plates of the R0040 and E0430 parts grew successfully. We prepared an overnight culture of two colonies from each plate, totalling 8 Falcon tubes. Each colony innoculated was also restreaked on new ampicillin plates in the event that we require them again. We also helped Himika help Emily miniprep her parts.
 
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Himika: Himika made overnight cultures from B0034 (the RBS that we all need) and B0015 (the double terminator for the end of all our circuits).
 
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Emily: Today I did gel electrophoresis of yesterday’s Colony PCR.  I also did a miniprep of yesterday’s overnight cultures of both I0500 and I13453 and I determined the concentration using the Nanodrop spectrophotometer.  I then performed a restriction digest with EcoRI and PstI.  This is another verification step for the arabinose promoters.  If it works, we would expect to see band sizes at ~ 1.4 kb (1210 bp for the gene + 200 bp for the Biobrick Prefix and Suffix) for I0500 as well as bands at ~ 330 bp (230 bp for the gene + 200 bp for the Biobrick Prefix and Suffix).  I also spent some time looking into what kind of genes of interest we could use to test out our system.  I’ve been looking into some human genes that have been found to misfold in E Coli.  These could work as a positive control, in order to demonstrate that our system is working.
 
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<h3>Check Another Date:</h3>
 
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<u>Chris, Jeremy, and Dev</u>
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Most of the growth on the plates were red, indicating that the placeholder gene was being transcribed and successfully produced. Our group spent today making overnight cultures so plasmid transfers could be done tomorrow. We made the cultures for plasmids pSB1A3, pSB1K2, pSB1AC3 and pSB1AK3 as well as restreaking the colonies that were used  to innoculate the overnight cultures. Jeremy also transformed I732005 (lacZ gene for B-galactosidase) into competent cells so they could be used.
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<u>Alex and Patrick</u>
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The ampicillin spread plates of the R0040 and E0430 parts grew successfully. We prepared an overnight culture of two colonies from each plate, totalling 8 Falcon tubes. Each colony innoculated was also restreaked on new ampicillin plates in the event that we require them again. We also helped Himika help Emily miniprep her parts.
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<u>Himika</u>
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I made overnight cultures from B0034 (the RBS that we all need) and B0015 (the double terminator for the end of all our circuits).
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<u>Emily</u>
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<a href="http://www.ucalgary.ca">&copy;2010 University of Calgary</a>
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Today I did gel electrophoresis of yesterday’s Colony PCR.  I also did a miniprep of yesterday’s overnight cultures of both I0500 and I13453 and I determined the concentration using the Nanodrop spectrophotometer.  I then performed a restriction digest with EcoRI and PstI.  This is another verification step for the arabinose promoters.  If it works, we would expect to see band sizes at ~ 1.4 kb (1210 bp for the gene + 200 bp for the Biobrick Prefix and Suffix) for I0500 as well as bands at ~ 330 bp (230 bp for the gene + 200 bp for the Biobrick Prefix and Suffix).  I also spent some time looking into what kind of genes of interest we could use to test out our system.  I’ve been looking into some human genes that have been found to misfold in E Coli.  These could work as a positive control, in order to demonstrate that our system is working.
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Latest revision as of 02:57, 23 August 2010

Notebook

May
MTWTFSS
          [http://2010.igem.org/Team:Calgary/1_May_2010 1] [http://2010.igem.org/Team:Calgary/2_May_2010 2]
[http://2010.igem.org/Team:Calgary/3_May_2010 3] [http://2010.igem.org/Team:Calgary/4_May_2010 4] [http://2010.igem.org/Team:Calgary/5_May_2010 5] [http://2010.igem.org/Team:Calgary/6_May_2010 6] [http://2010.igem.org/Team:Calgary/7_May_2010 7] [http://2010.igem.org/Team:Calgary/8_May_2010 8] [http://2010.igem.org/Team:Calgary/9_May_2010 9]
[http://2010.igem.org/Team:Calgary/10_May_2010 10] [http://2010.igem.org/Team:Calgary/11_May_2010 11] [http://2010.igem.org/Team:Calgary/12_May_2010 12] [http://2010.igem.org/Team:Calgary/13_May_2010 13] [http://2010.igem.org/Team:Calgary/14_May_2010 14] [http://2010.igem.org/Team:Calgary/15_May_2010 15] [http://2010.igem.org/Team:Calgary/16_May_2010 16]
[http://2010.igem.org/Team:Calgary/17_May_2010 17] [http://2010.igem.org/Team:Calgary/18_May_2010 18] [http://2010.igem.org/Team:Calgary/19_May_2010 19] [http://2010.igem.org/Team:Calgary/20_May_2010 20] [http://2010.igem.org/Team:Calgary/21_May_2010 21] [http://2010.igem.org/Team:Calgary/22_May_2010 22] [http://2010.igem.org/Team:Calgary/23_May_2010 23]
[http://2010.igem.org/Team:Calgary/24_May_2010 24] [http://2010.igem.org/Team:Calgary/25_May_2010 25] [http://2010.igem.org/Team:Calgary/26_May_2010 26] [http://2010.igem.org/Team:Calgary/27_May_2010 27] [http://2010.igem.org/Team:Calgary/28_May_2010 28] [http://2010.igem.org/Team:Calgary/29_May_2010 29] [http://2010.igem.org/Team:Calgary/30_May_2010 30]
[http://2010.igem.org/Team:Calgary/31_May_2010 31]

June
MTWTFSS
  [http://2010.igem.org/Team:Calgary/1_June_2010 1] [http://2010.igem.org/Team:Calgary/2_June_2010 2] [http://2010.igem.org/Team:Calgary/3_June_2010 3] [http://2010.igem.org/Team:Calgary/4_June_2010 4] [http://2010.igem.org/Team:Calgary/5_June_2010 5] [http://2010.igem.org/Team:Calgary/6_June_2010 6]
[http://2010.igem.org/Team:Calgary/7_June_2010 7] [http://2010.igem.org/Team:Calgary/8_June_2010 8] [http://2010.igem.org/Team:Calgary/9_June_2010 9] [http://2010.igem.org/Team:Calgary/10_June_2010 10] [http://2010.igem.org/Team:Calgary/11_June_2010 11] [http://2010.igem.org/Team:Calgary/12_June_2010 12] [http://2010.igem.org/Team:Calgary/13_June_2010 13]
[http://2010.igem.org/Team:Calgary/14_June_2010 14] [http://2010.igem.org/Team:Calgary/15_June_2010 15] [http://2010.igem.org/Team:Calgary/16_June_2010 16] [http://2010.igem.org/Team:Calgary/17_June_2010 17] [http://2010.igem.org/Team:Calgary/18_June_2010 18] [http://2010.igem.org/Team:Calgary/19_June_2010 19] [http://2010.igem.org/Team:Calgary/20_June_2010 20]
[http://2010.igem.org/Team:Calgary/21_June_2010 21] [http://2010.igem.org/Team:Calgary/22_June_2010 22] [http://2010.igem.org/Team:Calgary/23_June_2010 23] [http://2010.igem.org/Team:Calgary/24_June_2010 24] [http://2010.igem.org/Team:Calgary/25_June_2010 25] [http://2010.igem.org/Team:Calgary/26_June_2010 26] [http://2010.igem.org/Team:Calgary/27_June_2010 27]
[http://2010.igem.org/Team:Calgary/28_June_2010 28] [http://2010.igem.org/Team:Calgary/29_June_2010 29] [http://2010.igem.org/Team:Calgary/30_June_2010 30]

July
MTWTFSS
      [http://2010.igem.org/Team:Calgary/1_July_2010 1] [http://2010.igem.org/Team:Calgary/2_July_2010 2] [http://2010.igem.org/Team:Calgary/3_July_2010 3] [http://2010.igem.org/Team:Calgary/4_July_2010 4]
[http://2010.igem.org/Team:Calgary/5_July_2010 5] [http://2010.igem.org/Team:Calgary/6_July_2010 6] [http://2010.igem.org/Team:Calgary/7_July_2010 7] [http://2010.igem.org/Team:Calgary/8_July_2010 8] [http://2010.igem.org/Team:Calgary/9_July_2010 9] [http://2010.igem.org/Team:Calgary/10_July_2010 10] [http://2010.igem.org/Team:Calgary/11_July_2010 11]
[http://2010.igem.org/Team:Calgary/12_July_2010 12] [http://2010.igem.org/Team:Calgary/13_July_2010 13] [http://2010.igem.org/Team:Calgary/14_July_2010 14] [http://2010.igem.org/Team:Calgary/15_July_2010 15] [http://2010.igem.org/Team:Calgary/16_July_2010 16] [http://2010.igem.org/Team:Calgary/17_July_2010 17] [http://2010.igem.org/Team:Calgary/18_July_2010 18]
[http://2010.igem.org/Team:Calgary/19_July_2010 19] [http://2010.igem.org/Team:Calgary/20_July_2010 20] [http://2010.igem.org/Team:Calgary/21_July_2010 21] [http://2010.igem.org/Team:Calgary/22_July_2010 22] [http://2010.igem.org/Team:Calgary/23_July_2010 23] [http://2010.igem.org/Team:Calgary/24_July_2010 24] [http://2010.igem.org/Team:Calgary/25_July_2010 25]
[http://2010.igem.org/Team:Calgary/26_July_2010 26] [http://2010.igem.org/Team:Calgary/27_July_2010 27] [http://2010.igem.org/Team:Calgary/28_July_2010 28] [http://2010.igem.org/Team:Calgary/29_July_2010 29] [http://2010.igem.org/Team:Calgary/30_July_2010 30] [http://2010.igem.org/Team:Calgary/31_July_2010 31]

August
MTWTFSS
            [http://2010.igem.org/Team:Calgary/1_August_2010 1]
[http://2010.igem.org/Team:Calgary/2_August_2010 2] [http://2010.igem.org/Team:Calgary/3_August_2010 3] [http://2010.igem.org/Team:Calgary/4_August_2010 4] [http://2010.igem.org/Team:Calgary/5_August_2010 5] [http://2010.igem.org/Team:Calgary/6_August_2010 6] [http://2010.igem.org/Team:Calgary/7_August_2010 7] [http://2010.igem.org/Team:Calgary/8_August_2010 8]
[http://2010.igem.org/Team:Calgary/9_August_2010 9] [http://2010.igem.org/Team:Calgary/10_August_2010 10] [http://2010.igem.org/Team:Calgary/11_August_2010 11] [http://2010.igem.org/Team:Calgary/12_August_2010 12] [http://2010.igem.org/Team:Calgary/13_August_2010 13] [http://2010.igem.org/Team:Calgary/14_August_2010 14] [http://2010.igem.org/Team:Calgary/15_August_2010 15]
[http://2010.igem.org/Team:Calgary/16_August_2010 16] [http://2010.igem.org/Team:Calgary/17_August_2010 17] [http://2010.igem.org/Team:Calgary/18_August_2010 18] [http://2010.igem.org/Team:Calgary/19_August_2010 19] [http://2010.igem.org/Team:Calgary/20_August_2010 20] [http://2010.igem.org/Team:Calgary/21_August_2010 21] [http://2010.igem.org/Team:Calgary/22_August_2010 22]
[http://2010.igem.org/Team:Calgary/23_August_2010 23] [http://2010.igem.org/Team:Calgary/24_August_2010 24] [http://2010.igem.org/Team:Calgary/25_August_2010 25] [http://2010.igem.org/Team:Calgary/26_August_2010 26] [http://2010.igem.org/Team:Calgary/27_August_2010 27] [http://2010.igem.org/Team:Calgary/28_August_2010 28] [http://2010.igem.org/Team:Calgary/29_August_2010 29]
[http://2010.igem.org/Team:Calgary/30_August_2010 30] [http://2010.igem.org/Team:Calgary/31_August_2010 31]

September
MTWTFSS
    [http://2010.igem.org/Team:Calgary/1_September_2010 1] [http://2010.igem.org/Team:Calgary/2_September_2010 2] [http://2010.igem.org/Team:Calgary/3_September_2010 3] [http://2010.igem.org/Team:Calgary/4_September_2010 4] [http://2010.igem.org/Team:Calgary/5_September_2010 5]
[http://2010.igem.org/Team:Calgary/6_September_2010 6] [http://2010.igem.org/Team:Calgary/7_September_2010 7] [http://2010.igem.org/Team:Calgary/8_September_2010 8] [http://2010.igem.org/Team:Calgary/9_September_2010 9] [http://2010.igem.org/Team:Calgary/10_September_2010 10] [http://2010.igem.org/Team:Calgary/11_September_2010 11] [http://2010.igem.org/Team:Calgary/12_September_2010 12]
[http://2010.igem.org/Team:Calgary/13_September_2010 13] [http://2010.igem.org/Team:Calgary/14_September_2010 14] [http://2010.igem.org/Team:Calgary/15_September_2010 15] [http://2010.igem.org/Team:Calgary/16_September_2010 16] [http://2010.igem.org/Team:Calgary/17_September_2010 17] [http://2010.igem.org/Team:Calgary/18_September_2010 18] [http://2010.igem.org/Team:Calgary/19_September_2010 19]
[http://2010.igem.org/Team:Calgary/20_September_2010 20] [http://2010.igem.org/Team:Calgary/21_September_2010 21] [http://2010.igem.org/Team:Calgary/22_September_2010 22] [http://2010.igem.org/Team:Calgary/23_September_2010 23] [http://2010.igem.org/Team:Calgary/24_September_2010 24] [http://2010.igem.org/Team:Calgary/25_September_2010 25] [http://2010.igem.org/Team:Calgary/26_September_2010 26]
[http://2010.igem.org/Team:Calgary/27_September_2010 27] [http://2010.igem.org/Team:Calgary/28_September_2010 28] [http://2010.igem.org/Team:Calgary/29_September_2010 29] [http://2010.igem.org/Team:Calgary/30_September_2010 30]

October
MTWTFSS
        [http://2010.igem.org/Team:Calgary/1_October_2010 1] [http://2010.igem.org/Team:Calgary/2_October_2010 2] [http://2010.igem.org/Team:Calgary/3_October_2010 3]
[http://2010.igem.org/Team:Calgary/4_October_2010 4] [http://2010.igem.org/Team:Calgary/5_October_2010 5] [http://2010.igem.org/Team:Calgary/6_October_2010 6] [http://2010.igem.org/Team:Calgary/7_October_2010 7] [http://2010.igem.org/Team:Calgary/8_October_2010 8] [http://2010.igem.org/Team:Calgary/9_October_2010 9] [http://2010.igem.org/Team:Calgary/10_October_2010 10]
[http://2010.igem.org/Team:Calgary/11_October_2010 11] [http://2010.igem.org/Team:Calgary/12_October_2010 12] [http://2010.igem.org/Team:Calgary/13_October_2010 13] [http://2010.igem.org/Team:Calgary/14_October_2010 14] [http://2010.igem.org/Team:Calgary/15_October_2010 15] [http://2010.igem.org/Team:Calgary/16_October_2010 16] [http://2010.igem.org/Team:Calgary/17_October_2010 17]
[http://2010.igem.org/Team:Calgary/18_October_2010 18] [http://2010.igem.org/Team:Calgary/19_October_2010 19] [http://2010.igem.org/Team:Calgary/20_October_2010 20] [http://2010.igem.org/Team:Calgary/21_October_2010 21] [http://2010.igem.org/Team:Calgary/22_October_2010 22] [http://2010.igem.org/Team:Calgary/23_October_2010 23] [http://2010.igem.org/Team:Calgary/24_October_2010 24]
[http://2010.igem.org/Team:Calgary/25_October_2010 25] [http://2010.igem.org/Team:Calgary/26_October_2010 26] [http://2010.igem.org/Team:Calgary/27_October_2010 27] [http://2010.igem.org/Team:Calgary/28_October_2010 28] [http://2010.igem.org/Team:Calgary/29_October_2010 29] [http://2010.igem.org/Team:Calgary/30_October_2010 30] [http://2010.igem.org/Team:Calgary/31_October_2010 31]

Tuesday June 8, 2010

Dev, Jeremy, and Chris's spread plates of Ampicillin - Chloramphenicol and Ampicillin - Kanamycin from the Parts Registry.
Dev, Jeremy, and Chris's spread plates of the Ampicillin and Kanamycin plasmid backbones from the Parts Registry.
Alex's and Patrick's spread plates. Photograph taken at 8:48am June 8th, 2010.

Today, we spent most of the day making overnight cultures from the transformed plates that were made yesterday. As a group, we began getting fundraising underway by finding contacts, dividng them among each person and setting a tentative goal of $20 000 to fundraise from corporate sponsors. We also organized how the money given from Alberta Innovates would be used


Chris, Jeremy, and Dev

Most of the growth on the plates were red, indicating that the placeholder gene was being transcribed and successfully produced. Our group spent today making overnight cultures so plasmid transfers could be done tomorrow. We made the cultures for plasmids pSB1A3, pSB1K2, pSB1AC3 and pSB1AK3 as well as restreaking the colonies that were used to innoculate the overnight cultures. Jeremy also transformed I732005 (lacZ gene for B-galactosidase) into competent cells so they could be used.


Alex and Patrick

The ampicillin spread plates of the R0040 and E0430 parts grew successfully. We prepared an overnight culture of two colonies from each plate, totalling 8 Falcon tubes. Each colony innoculated was also restreaked on new ampicillin plates in the event that we require them again. We also helped Himika help Emily miniprep her parts.


Himika

I made overnight cultures from B0034 (the RBS that we all need) and B0015 (the double terminator for the end of all our circuits).


Emily

Today I did gel electrophoresis of yesterday’s Colony PCR. I also did a miniprep of yesterday’s overnight cultures of both I0500 and I13453 and I determined the concentration using the Nanodrop spectrophotometer. I then performed a restriction digest with EcoRI and PstI. This is another verification step for the arabinose promoters. If it works, we would expect to see band sizes at ~ 1.4 kb (1210 bp for the gene + 200 bp for the Biobrick Prefix and Suffix) for I0500 as well as bands at ~ 330 bp (230 bp for the gene + 200 bp for the Biobrick Prefix and Suffix). I also spent some time looking into what kind of genes of interest we could use to test out our system. I’ve been looking into some human genes that have been found to misfold in E Coli. These could work as a positive control, in order to demonstrate that our system is working.

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