Team:Calgary/10 October 2010

From 2010.igem.org

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[[Image:10.11.2010.Jeremy's Various Constructs pt1.jpg|200px|thumb|right|I13504+I0500+B0034, I13507+I0500+B0034
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[[Image:10.11.2010.Jeremy's pt2 Including PRFP EcoSpe Digest.jpg|200px|thumb|right|I13507+I0500+B0034+MalE31 and sRFP
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<u>Dev</u>
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The second podcast in the University of Calgary's Ethics podcasts series was recorded today. We discuss the consequences of open source synthetic biology as well as point out some of it's advantages.
 +
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<u>Jeremy</u>
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Today I ran a gel of the materials from the colony PCR. The first gel wells 2-6 are I13504+I0500+B0034 colonies, wells 7-11 are I13507+I0500+B0034 and 12-14 are I13507+I0500+B0034 colonies from a different plate. For the second gel, wells 2-8 were different colonies of pRFP and well 9-14 were colonies of I13507+I0500+B0034+MalE31. All the I13504+I0500+B0034 look promising, at least one I13507+I0500+B0034 from well 8 looks promising. For the RFP well 6 looks promising and all the I13507+I0500+B0034+MalE31 looks good.
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Latest revision as of 18:54, 27 October 2010

Notebook

May
MTWTFSS
          [http://2010.igem.org/Team:Calgary/1_May_2010 1] [http://2010.igem.org/Team:Calgary/2_May_2010 2]
[http://2010.igem.org/Team:Calgary/3_May_2010 3] [http://2010.igem.org/Team:Calgary/4_May_2010 4] [http://2010.igem.org/Team:Calgary/5_May_2010 5] [http://2010.igem.org/Team:Calgary/6_May_2010 6] [http://2010.igem.org/Team:Calgary/7_May_2010 7] [http://2010.igem.org/Team:Calgary/8_May_2010 8] [http://2010.igem.org/Team:Calgary/9_May_2010 9]
[http://2010.igem.org/Team:Calgary/10_May_2010 10] [http://2010.igem.org/Team:Calgary/11_May_2010 11] [http://2010.igem.org/Team:Calgary/12_May_2010 12] [http://2010.igem.org/Team:Calgary/13_May_2010 13] [http://2010.igem.org/Team:Calgary/14_May_2010 14] [http://2010.igem.org/Team:Calgary/15_May_2010 15] [http://2010.igem.org/Team:Calgary/16_May_2010 16]
[http://2010.igem.org/Team:Calgary/17_May_2010 17] [http://2010.igem.org/Team:Calgary/18_May_2010 18] [http://2010.igem.org/Team:Calgary/19_May_2010 19] [http://2010.igem.org/Team:Calgary/20_May_2010 20] [http://2010.igem.org/Team:Calgary/21_May_2010 21] [http://2010.igem.org/Team:Calgary/22_May_2010 22] [http://2010.igem.org/Team:Calgary/23_May_2010 23]
[http://2010.igem.org/Team:Calgary/24_May_2010 24] [http://2010.igem.org/Team:Calgary/25_May_2010 25] [http://2010.igem.org/Team:Calgary/26_May_2010 26] [http://2010.igem.org/Team:Calgary/27_May_2010 27] [http://2010.igem.org/Team:Calgary/28_May_2010 28] [http://2010.igem.org/Team:Calgary/29_May_2010 29] [http://2010.igem.org/Team:Calgary/30_May_2010 30]
[http://2010.igem.org/Team:Calgary/31_May_2010 31]

June
MTWTFSS
  [http://2010.igem.org/Team:Calgary/1_June_2010 1] [http://2010.igem.org/Team:Calgary/2_June_2010 2] [http://2010.igem.org/Team:Calgary/3_June_2010 3] [http://2010.igem.org/Team:Calgary/4_June_2010 4] [http://2010.igem.org/Team:Calgary/5_June_2010 5] [http://2010.igem.org/Team:Calgary/6_June_2010 6]
[http://2010.igem.org/Team:Calgary/7_June_2010 7] [http://2010.igem.org/Team:Calgary/8_June_2010 8] [http://2010.igem.org/Team:Calgary/9_June_2010 9] [http://2010.igem.org/Team:Calgary/10_June_2010 10] [http://2010.igem.org/Team:Calgary/11_June_2010 11] [http://2010.igem.org/Team:Calgary/12_June_2010 12] [http://2010.igem.org/Team:Calgary/13_June_2010 13]
[http://2010.igem.org/Team:Calgary/14_June_2010 14] [http://2010.igem.org/Team:Calgary/15_June_2010 15] [http://2010.igem.org/Team:Calgary/16_June_2010 16] [http://2010.igem.org/Team:Calgary/17_June_2010 17] [http://2010.igem.org/Team:Calgary/18_June_2010 18] [http://2010.igem.org/Team:Calgary/19_June_2010 19] [http://2010.igem.org/Team:Calgary/20_June_2010 20]
[http://2010.igem.org/Team:Calgary/21_June_2010 21] [http://2010.igem.org/Team:Calgary/22_June_2010 22] [http://2010.igem.org/Team:Calgary/23_June_2010 23] [http://2010.igem.org/Team:Calgary/24_June_2010 24] [http://2010.igem.org/Team:Calgary/25_June_2010 25] [http://2010.igem.org/Team:Calgary/26_June_2010 26] [http://2010.igem.org/Team:Calgary/27_June_2010 27]
[http://2010.igem.org/Team:Calgary/28_June_2010 28] [http://2010.igem.org/Team:Calgary/29_June_2010 29] [http://2010.igem.org/Team:Calgary/30_June_2010 30]

July
MTWTFSS
      [http://2010.igem.org/Team:Calgary/1_July_2010 1] [http://2010.igem.org/Team:Calgary/2_July_2010 2] [http://2010.igem.org/Team:Calgary/3_July_2010 3] [http://2010.igem.org/Team:Calgary/4_July_2010 4]
[http://2010.igem.org/Team:Calgary/5_July_2010 5] [http://2010.igem.org/Team:Calgary/6_July_2010 6] [http://2010.igem.org/Team:Calgary/7_July_2010 7] [http://2010.igem.org/Team:Calgary/8_July_2010 8] [http://2010.igem.org/Team:Calgary/9_July_2010 9] [http://2010.igem.org/Team:Calgary/10_July_2010 10] [http://2010.igem.org/Team:Calgary/11_July_2010 11]
[http://2010.igem.org/Team:Calgary/12_July_2010 12] [http://2010.igem.org/Team:Calgary/13_July_2010 13] [http://2010.igem.org/Team:Calgary/14_July_2010 14] [http://2010.igem.org/Team:Calgary/15_July_2010 15] [http://2010.igem.org/Team:Calgary/16_July_2010 16] [http://2010.igem.org/Team:Calgary/17_July_2010 17] [http://2010.igem.org/Team:Calgary/18_July_2010 18]
[http://2010.igem.org/Team:Calgary/19_July_2010 19] [http://2010.igem.org/Team:Calgary/20_July_2010 20] [http://2010.igem.org/Team:Calgary/21_July_2010 21] [http://2010.igem.org/Team:Calgary/22_July_2010 22] [http://2010.igem.org/Team:Calgary/23_July_2010 23] [http://2010.igem.org/Team:Calgary/24_July_2010 24] [http://2010.igem.org/Team:Calgary/25_July_2010 25]
[http://2010.igem.org/Team:Calgary/26_July_2010 26] [http://2010.igem.org/Team:Calgary/27_July_2010 27] [http://2010.igem.org/Team:Calgary/28_July_2010 28] [http://2010.igem.org/Team:Calgary/29_July_2010 29] [http://2010.igem.org/Team:Calgary/30_July_2010 30] [http://2010.igem.org/Team:Calgary/31_July_2010 31]

August
MTWTFSS
            [http://2010.igem.org/Team:Calgary/1_August_2010 1]
[http://2010.igem.org/Team:Calgary/2_August_2010 2] [http://2010.igem.org/Team:Calgary/3_August_2010 3] [http://2010.igem.org/Team:Calgary/4_August_2010 4] [http://2010.igem.org/Team:Calgary/5_August_2010 5] [http://2010.igem.org/Team:Calgary/6_August_2010 6] [http://2010.igem.org/Team:Calgary/7_August_2010 7] [http://2010.igem.org/Team:Calgary/8_August_2010 8]
[http://2010.igem.org/Team:Calgary/9_August_2010 9] [http://2010.igem.org/Team:Calgary/10_August_2010 10] [http://2010.igem.org/Team:Calgary/11_August_2010 11] [http://2010.igem.org/Team:Calgary/12_August_2010 12] [http://2010.igem.org/Team:Calgary/13_August_2010 13] [http://2010.igem.org/Team:Calgary/14_August_2010 14] [http://2010.igem.org/Team:Calgary/15_August_2010 15]
[http://2010.igem.org/Team:Calgary/16_August_2010 16] [http://2010.igem.org/Team:Calgary/17_August_2010 17] [http://2010.igem.org/Team:Calgary/18_August_2010 18] [http://2010.igem.org/Team:Calgary/19_August_2010 19] [http://2010.igem.org/Team:Calgary/20_August_2010 20] [http://2010.igem.org/Team:Calgary/21_August_2010 21] [http://2010.igem.org/Team:Calgary/22_August_2010 22]
[http://2010.igem.org/Team:Calgary/23_August_2010 23] [http://2010.igem.org/Team:Calgary/24_August_2010 24] [http://2010.igem.org/Team:Calgary/25_August_2010 25] [http://2010.igem.org/Team:Calgary/26_August_2010 26] [http://2010.igem.org/Team:Calgary/27_August_2010 27] [http://2010.igem.org/Team:Calgary/28_August_2010 28] [http://2010.igem.org/Team:Calgary/29_August_2010 29]
[http://2010.igem.org/Team:Calgary/30_August_2010 30] [http://2010.igem.org/Team:Calgary/31_August_2010 31]

September
MTWTFSS
    [http://2010.igem.org/Team:Calgary/1_September_2010 1] [http://2010.igem.org/Team:Calgary/2_September_2010 2] [http://2010.igem.org/Team:Calgary/3_September_2010 3] [http://2010.igem.org/Team:Calgary/4_September_2010 4] [http://2010.igem.org/Team:Calgary/5_September_2010 5]
[http://2010.igem.org/Team:Calgary/6_September_2010 6] [http://2010.igem.org/Team:Calgary/7_September_2010 7] [http://2010.igem.org/Team:Calgary/8_September_2010 8] [http://2010.igem.org/Team:Calgary/9_September_2010 9] [http://2010.igem.org/Team:Calgary/10_September_2010 10] [http://2010.igem.org/Team:Calgary/11_September_2010 11] [http://2010.igem.org/Team:Calgary/12_September_2010 12]
[http://2010.igem.org/Team:Calgary/13_September_2010 13] [http://2010.igem.org/Team:Calgary/14_September_2010 14] [http://2010.igem.org/Team:Calgary/15_September_2010 15] [http://2010.igem.org/Team:Calgary/16_September_2010 16] [http://2010.igem.org/Team:Calgary/17_September_2010 17] [http://2010.igem.org/Team:Calgary/18_September_2010 18] [http://2010.igem.org/Team:Calgary/19_September_2010 19]
[http://2010.igem.org/Team:Calgary/20_September_2010 20] [http://2010.igem.org/Team:Calgary/21_September_2010 21] [http://2010.igem.org/Team:Calgary/22_September_2010 22] [http://2010.igem.org/Team:Calgary/23_September_2010 23] [http://2010.igem.org/Team:Calgary/24_September_2010 24] [http://2010.igem.org/Team:Calgary/25_September_2010 25] [http://2010.igem.org/Team:Calgary/26_September_2010 26]
[http://2010.igem.org/Team:Calgary/27_September_2010 27] [http://2010.igem.org/Team:Calgary/28_September_2010 28] [http://2010.igem.org/Team:Calgary/29_September_2010 29] [http://2010.igem.org/Team:Calgary/30_September_2010 30]

October
MTWTFSS
        [http://2010.igem.org/Team:Calgary/1_October_2010 1] [http://2010.igem.org/Team:Calgary/2_October_2010 2] [http://2010.igem.org/Team:Calgary/3_October_2010 3]
[http://2010.igem.org/Team:Calgary/4_October_2010 4] [http://2010.igem.org/Team:Calgary/5_October_2010 5] [http://2010.igem.org/Team:Calgary/6_October_2010 6] [http://2010.igem.org/Team:Calgary/7_October_2010 7] [http://2010.igem.org/Team:Calgary/8_October_2010 8] [http://2010.igem.org/Team:Calgary/9_October_2010 9] [http://2010.igem.org/Team:Calgary/10_October_2010 10]
[http://2010.igem.org/Team:Calgary/11_October_2010 11] [http://2010.igem.org/Team:Calgary/12_October_2010 12] [http://2010.igem.org/Team:Calgary/13_October_2010 13] [http://2010.igem.org/Team:Calgary/14_October_2010 14] [http://2010.igem.org/Team:Calgary/15_October_2010 15] [http://2010.igem.org/Team:Calgary/16_October_2010 16] [http://2010.igem.org/Team:Calgary/17_October_2010 17]
[http://2010.igem.org/Team:Calgary/18_October_2010 18] [http://2010.igem.org/Team:Calgary/19_October_2010 19] [http://2010.igem.org/Team:Calgary/20_October_2010 20] [http://2010.igem.org/Team:Calgary/21_October_2010 21] [http://2010.igem.org/Team:Calgary/22_October_2010 22] [http://2010.igem.org/Team:Calgary/23_October_2010 23] [http://2010.igem.org/Team:Calgary/24_October_2010 24]
[http://2010.igem.org/Team:Calgary/25_October_2010 25] [http://2010.igem.org/Team:Calgary/26_October_2010 26] [http://2010.igem.org/Team:Calgary/27_October_2010 27] [http://2010.igem.org/Team:Calgary/28_October_2010 28] [http://2010.igem.org/Team:Calgary/29_October_2010 29] [http://2010.igem.org/Team:Calgary/30_October_2010 30] [http://2010.igem.org/Team:Calgary/31_October_2010 31]

Sunday October 10, 2010

I13504+I0500+B0034, I13507+I0500+B0034
I13507+I0500+B0034+MalE31 and sRFP


Dev

The second podcast in the University of Calgary's Ethics podcasts series was recorded today. We discuss the consequences of open source synthetic biology as well as point out some of it's advantages.

Jeremy

Today I ran a gel of the materials from the colony PCR. The first gel wells 2-6 are I13504+I0500+B0034 colonies, wells 7-11 are I13507+I0500+B0034 and 12-14 are I13507+I0500+B0034 colonies from a different plate. For the second gel, wells 2-8 were different colonies of pRFP and well 9-14 were colonies of I13507+I0500+B0034+MalE31. All the I13504+I0500+B0034 look promising, at least one I13507+I0500+B0034 from well 8 looks promising. For the RFP well 6 looks promising and all the I13507+I0500+B0034+MalE31 looks good.


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