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-=Polymerase Chain action protocol=
+=Polymerase Chain Reaction=
-Polymerase chain reaction (PCR) is a technique used to amplify a single or few a copies of a piece of DNA generating thousands to millions of copies of a particular DNA sequence.
+==Aim==
-# To avoid contamination, wear gloves
-# To achieve optimum results, always do everything on ice
-#
+To amplify a desired DNA fragment by using a set of appropriate primers flanking the fragment.
+==Materials and Protocol==
+Please refer to: [[Team:Newcastle/PCR| PCR]] for materials required and protocol.
+[[Image:8062010.jpg|400px|center]]
+'''Image 1''': Image of the gel showing PCR products.
+* '''Lane 1''': 1kb DNA ladder
+* '''Lane 2''': Sample 1
+* '''Lane 3''': Sample 2
+* '''Lane 4''': Sample 3
+* '''Lane 5''': Sample 4
+* '''Lane 6''': Sample 5
+* '''Lane 7''': Sample 6
+* '''Lane 8''': Sample 7
+* '''Lane 9''': Sample 8
+* '''Lane 10''': Sample 9
+* '''Lane 11''': Sample 10
+* '''Lane 12''': Sample 11
+* '''Lane 13''': Sample 12
+* '''Lane 14''': Sample 13
+* '''Lane 15''': Sample 14
 {{Team:Newcastle/footer}}

Team:Newcastle/18 June 2010

Latest revision as of 14:08, 27 October 2010