Team:TU Delft/Tour3
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(New page: <html> <!-- Tour START /--> <style> #breadcrumbs { display: none; } #tudelftigemtour { overflow: auto; } #tourmenu { float: left; width: 200px; border: 1px solid #ffd200; margin: 0...) |
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- | <a href=" | + | <a href="https://2010.igem.org/Team:TU_Delft#page=Tour2">« Back</a> |
</div> | </div> | ||
<div id="next"> | <div id="next"> | ||
- | <a href=" | + | <a href="https://2010.igem.org/Team:TU_Delft#page=Tour4">Continue »</a> |
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<div class="tour-content-block"> | <div class="tour-content-block"> | ||
<div id="img" class="right"> | <div id="img" class="right"> | ||
- | <img src="" width=" | + | <img src="https://static.igem.org/mediawiki/2010/0/09/TU_Delft_Tour3_degradation.png" width="300" /> |
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<h3>Octane, octanol, octanal...</h3> | <h3>Octane, octanol, octanal...</h3> | ||
<p>Lower peaks on the GC graphs made us very proud. The biobricks reduce alkanes up to C30 to thin air.</p><p>Enzyme assays confirm that it is not just evaporation that we are looking at.</p> | <p>Lower peaks on the GC graphs made us very proud. The biobricks reduce alkanes up to C30 to thin air.</p><p>Enzyme assays confirm that it is not just evaporation that we are looking at.</p> | ||
- | <p><a href="">Read more</a></p> | + | <p><a href="https://2010.igem.org/Team:TU_Delft/Project/alkane-degradation/results">Read more</a></p> |
</div> | </div> | ||
</div> | </div> | ||
<div class="tour-content-block"> | <div class="tour-content-block"> | ||
<div id="img" class="left"> | <div id="img" class="left"> | ||
- | <img src="" width=" | + | <img src="https://static.igem.org/mediawiki/2010/0/07/TU_Delft_Tour3_survival_graph.png" width="300" /> |
</div> | </div> | ||
<div id="text"> | <div id="text"> | ||
<h3>Higher slopes equals happy cells</h3> | <h3>Higher slopes equals happy cells</h3> | ||
- | <p>The tolerance bricks were characterized by measuring growth curves. Once transformed, the cells survived up to | + | <p>The tolerance bricks were characterized by measuring growth curves. Once transformed, the cells survived up to 1 molar of salt and 10% of n-hexane.</p><p>Clearly, our cells started to enjoy these conditions.</p> |
- | <p><a href="">Read more</a></p> | + | <p><a href="https://2010.igem.org/Team:TU_Delft/Project/survival/results">Read more</a></p> |
</div> | </div> | ||
</div> | </div> | ||
<div class="tour-content-block"> | <div class="tour-content-block"> | ||
<div id="img" class="right"> | <div id="img" class="right"> | ||
- | <img src="" width=" | + | <img src="https://static.igem.org/mediawiki/2010/1/18/TU_Delft_Tour3_sensing.png" width="300" /> |
</div> | </div> | ||
<div id="text"> | <div id="text"> | ||
- | <h3>Shiny GFP on | + | <h3>Shiny GFP on glucose depletion</h3> |
- | <p>The sensing parts were connected to a GFP reporter. Our cells could detect the | + | <p>The sensing parts were connected to a GFP reporter. Our cells could detect the absence of glucose.</p> |
- | <p><a href="">Read more</a></p> | + | <p><a href="https://2010.igem.org/Team:TU_Delft/Project/sensing/results">Read more</a></p> |
</div> | </div> | ||
</div> | </div> | ||
<div class="tour-content-block"> | <div class="tour-content-block"> | ||
<div id="img" class="left"> | <div id="img" class="left"> | ||
- | <img src="" width=" | + | <img src="https://static.igem.org/mediawiki/2010/5/56/TU_Delft_Tour3_emulsifier.png" width="300" /> |
</div> | </div> | ||
<div id="text"> | <div id="text"> | ||
<h3>New! Emulsification Assay</h3> | <h3>New! Emulsification Assay</h3> | ||
<p>We created a new assay for the measurement of emulsification assays. Thanks to the hydrophobic dye Sudan II we could determine the strength of the emulsifier.</p> | <p>We created a new assay for the measurement of emulsification assays. Thanks to the hydrophobic dye Sudan II we could determine the strength of the emulsifier.</p> | ||
- | <p><a href="">Read more</a></p> | + | <p><a href="https://2010.igem.org/Team:TU_Delft/Project/solubility/results">Read more</a></p> |
</div> | </div> | ||
</div> | </div> | ||
<div id="tour-navigation"> | <div id="tour-navigation"> | ||
<div id="previous"> | <div id="previous"> | ||
- | <a href=" | + | <a href="https://2010.igem.org/Team:TU_Delft#page=Tour2">« Back</a> |
</div> | </div> | ||
<div id="next"> | <div id="next"> | ||
- | <a href=" | + | <a href="https://2010.igem.org/Team:TU_Delft#page=Tour4">Continue »</a> |
</div> | </div> | ||
</div> | </div> |
Latest revision as of 10:16, 27 October 2010
Characterization
After cloning comes characterization. We just love it when a plan comes together!
Octane, octanol, octanal...
Lower peaks on the GC graphs made us very proud. The biobricks reduce alkanes up to C30 to thin air.
Enzyme assays confirm that it is not just evaporation that we are looking at.
Higher slopes equals happy cells
The tolerance bricks were characterized by measuring growth curves. Once transformed, the cells survived up to 1 molar of salt and 10% of n-hexane.
Clearly, our cells started to enjoy these conditions.
Shiny GFP on glucose depletion
The sensing parts were connected to a GFP reporter. Our cells could detect the absence of glucose.
New! Emulsification Assay
We created a new assay for the measurement of emulsification assays. Thanks to the hydrophobic dye Sudan II we could determine the strength of the emulsifier.